Hydrophilic interaction chromatography: a guide for practitioners
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Hoboken, N.J.
Wiley
2013
|
| Schriftenreihe: | Chemical analysis
177 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Klappentext |
| Beschreibung: | XVI, 313 S. Ill., graph. Darst. |
| ISBN: | 9781118054178 |
Internformat
MARC
| LEADER | 00000nam a2200000 cb4500 | ||
|---|---|---|---|
| 001 | BV040899654 | ||
| 003 | DE-604 | ||
| 005 | 20130507 | ||
| 007 | t | ||
| 008 | 130320s2013 ad|| |||| 00||| eng d | ||
| 020 | |a 9781118054178 |c (hbk.) £66.95 |9 978-1-118-05417-8 | ||
| 035 | |a (OCoLC)840765310 | ||
| 035 | |a (DE-599)OBVAC10724337 | ||
| 040 | |a DE-604 |b ger | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-11 |a DE-355 |a DE-19 | ||
| 082 | 0 | |a 543.84 | |
| 084 | |a VG 7500 |0 (DE-625)147206:253 |2 rvk | ||
| 084 | |a WC 3480 |0 (DE-625)148087: |2 rvk | ||
| 245 | 1 | 0 | |a Hydrophilic interaction chromatography |b a guide for practitioners |c ed. by Bernard A. Olsen ... |
| 264 | 1 | |a Hoboken, N.J. |b Wiley |c 2013 | |
| 300 | |a XVI, 313 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Chemical analysis |v 177 | |
| 650 | 0 | 7 | |a Hydrophile Interaktions-Flüssigchromatographie |0 (DE-588)7863259-6 |2 gnd |9 rswk-swf |
| 653 | |a Hydrophilic interaction liquid chromatography. | ||
| 689 | 0 | 0 | |a Hydrophile Interaktions-Flüssigchromatographie |0 (DE-588)7863259-6 |D s |
| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a Olsen, Bernard A. |4 edt | |
| 830 | 0 | |a Chemical analysis |v 177 |w (DE-604)BV000008780 |9 177 | |
| 856 | 4 | 2 | |m Digitalisierung UB Regensburg |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025879162&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 856 | 4 | 2 | |m Digitalisierung UB Regensburg |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025879162&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA |3 Klappentext |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-025879162 | ||
Datensatz im Suchindex
| _version_ | 1804150185295609856 |
|---|---|
| adam_text | CONTENTS
PREFACE
xiii
CONTRIBUTORS
xv
CHAPTER
1
SEPARATION MECHANISMS IN
HYDROPHILIC INTERACTION
CHROMATOGRAPHY
1
David V. McCalley
1.1
Introduction
1
1.2
Historical Background: Recognition of the
Contribution of Partition, Ion Exchange, and
RP Interactions to the Retention Process
3
1.3
Recent Studies on the Contributory Mechanisms
to HILIC Retention
7
1.3.1
Overview
7
1.3.2
Contribution of Adsorption and Partition
to HILIC Separations
14
1.3.3
Further Studies on the Contribution of
Ionic Retention in HILIC
20
1.3.3.1
Introduction
20
1.3.3.2
Mobile Phase Considerations for
the Separation of Ionogenic
Compounds
21
1.3.3.3
lonization State of the Column as
a Function of
pH 23
1.3.3.4
Quantitation of Ionic Retention
Effects on Different Columns
26
1.3.4
RP Retention on Bare Silica
33
1.3.5
Electrostatic Repulsion Hydrophilic
Interaction Chromatography (ERLIC):
A New Separation Mode in HILIC
35
1.4
Conclusions
38
References
38
VI
CONTENTS
CHAPTER
2
STATIONARY PHASES FOR HILIC
43
Mohammed E.A. Ibrahim and Charles A. Lucy
2.1
Introduction
43
2.2
HILIC Stationary Phases
44
2.2.1
Underivatized Silica
44
2.2.1.1
Totally Porous Silica Particles
45
2.2.1.2
Superficially Porous (Core Shell)
Silica Particles
50
2.2.1.3
Monolithic Silica
50
2.2.1.4
Ethylene
Bridged Hybrids
(BEH)
51
2.2.2
Derivatized Silica
52
2.2.2.1
Neutral Derivatized Silica
52
2.2.2.2
Zwitterionic Derivatized Silica
56
2.2.2.3
Positively Charged Derivatized Silica
58
2.2.2.4
Negatively Charged Derivatized Silica
59
2.2.3
Nonsilica Phases
61
2.2.3.1
Amino
Phases
61
2.2.3.2
Sulfonated S-DVB Phases
61
2.3
Commercial HILIC Phases
61
2.3.1
Efficiency Comparison
62
2.3.2
Retention and Selectivity Comparisons
71
2.4
Conclusions
77
Acknowledgments
77
References
77
CHAPTER
3
HILIC METHOD DEVELOPMENT
87
Yong Guo and Xiande Wang
3.1
Introduction
87
3.2
General Method Development Considerations
88
3.2.1
Method Objectives
88
3.2.2
Target Compounds Consideration
90
3.2.3
Systematic Method Development
91
3.3
HILIC Method Development
93
3.3.1
Systematic Approach to Column
Screening
93
3.3.2
Optimization of Method Parameters
97
3.3.2.1
Final Column Selection
97
3.3.2.2
Organic Solvents
99
3.3.2.3
Mobile Phase
pH 99
CONTENTS
Vil
3.3.2.4 Buffer
Types and Concentration
101
3.3.2.5
Column Temperature
103
3.3.2.6
Sample Solvents
103
3.4
Detection for HILIC Methods
104
3.4.1
MS Detector
104
3.4.2
CAD
107
3.5
Conclusions
107
References
108
CHAPTER
4
PHARMACEUTICAL APPLICATIONS
OF HYDROPHILIC INTERACTION
CHROMATOGRAPHY 111
Bernard A.
Olsen,
Donald S. Risley, V. Scott Sharp,
Brian W. Pack, and Michelle L. Lytle
4.1
Introduction
112
4.1.1
Definition of the Problem
114
4.1.2
Selection of Conditions
115
4.1.3
Validation of the Method
117
4.1.4
General References
117
4.2
Determination of Counterions
117
4.2.1
Salt Selection and Options for
Counterion
Determination
117
4.2.2
Specific
Counterion
Analysis
120
4.2.3
Counterion
Screening with
Gradient Elution
125
4.2.4
Suitable Reference Standards for
Counterion
Analysis
128
4.3
Main Component Methods
129
4.3.1
Potency/Assay Methods
129
4.3.2
Equipment Cleaning Verification Assays
131
4.3.3
Dissolution Methods
133
4.4
Determination of Impurities
135
4.4.1
Impurity Screening and Orthogonal
Separations
135
4.4.2
Impurity Identification
138
4.4.3
Specific Impurity Determination
138
4.4.3.1
Pyrimidines,
Purines,
and
Nucleosides
138
4.4.3.2
Hydrazines with
Ethanol
as
Weak Solvent
140
Vlil
CONTENTS
4.4.3.3
Neutral
and Charged Polar
Impurities in a Drug Substance
141
4.4.3.4
Polar Basic Compounds and
Impurities
143
4.4.4
Statistical DOE for Optimization
144
4.5
Excipients
146
4.5.1
Parenteral and Solution Formulations
146
4.5.2
Tablets, Capsules, and
Inhalation Products
148
4.5.3
Sugars
150
4.5.4
Stabilizers and Antioxidants
150
4.6
Chiral Applications
152
4.6.1
Chiral Selectors and HILIC
153
4.6.1.1
Cyclodextrins
153
4.6.1.2
Macrocyclic Antibiotics
154
4.6.1.3
Chiral Crown Ethers
156
4.6.1.4
Cyclofructans
158
4.6.2
Conclusions for Chiral Separations
160
4.7
Conclusions
161
References
161
CHAPTER
5
HYDROPHILIC INTERACTION
CHROMATOGRAPHY (HILIC)
FOR DRUG DISCOVERY
169
Alfonso
Espada
and Mark Strege
5.1
Drug Discovery Model
169
5.2
HILIC Applications for In Vitro Biology
170
5.2.1
Biological Screening and Hit Finding
170
5.2.1.1
Target Selection and Assay Validation
171
5.2.1.2
High-Throughput Screening
(HTS)
173
5.2.2
New Drug Discovery Strategies
174
5.3
HILIC Applications for Discovery Chemistry
176
5.3.1
Lead Identification
176
5.3.2
Lead Optimization
180
5.3.2.1
ADME Profile
181
5.3.2.2
Biopharmaceutics
181
5.3.2.3
Chiral Purity
183
5.3.3
Candidate Selection
183
5.4
Practical Considerations
186
5.5
Conclusions
,. 187
References
188
CONTENTS
ЇХ
CHAPTER
6
ADVANCES IN HYDROPHILIC INTERACTION
CHROMATOGRAPHY (HILIC) FOR
BIOCHEMICAL APPLICATIONS
195
Fred
Rabel
and Bernard A.
Olsen
6.1
Introduction
195
6.2
Carbohydrates
196
6.2.1
Mono- and Disaccharides
196
6.2.2
Oligosaccharides and Polysaccharides
197
6.2.3
Glycans
197
6.2.3.1
Glycan and Glycopeptide Analysis
199
6.2.3.2
HILIC for Sample Enrichment
202
6.3
Nucleobases and Nucleosides
203
6.4
Oligonucleotides
205
6.5
Amino
Acids and Peptides
206
6.6
Proteins
209
6.7
Phospholipids
209
6.8
Conclusions
211
References
212
CHAPTER
7
HILIC-MS FOR TARGETED METABOLOMICS
AND SMALL MOLECULE
BIOANALYSIS
219
Hien
P. Nguyen, Heather D.
Tippens,
and Kevin A. Schug
7.1
Introduction
219
7.2
The Role of HILIC-MS in Targeted
Metabolomics versus Other LC Modes
221
7.3
Strategies for Method Development Based on
Retention Behavior of Targeted Metabolites on
HILIC Stationary Phases
223
7.3.1
Retention Behavior of Metabolites on
HILIC Stationary Phases
225
7.3.2
Robustness, Mobile Phase Compositions,
and Matrix Effects
227
7.4
Summary
231
Acknowledgments
232
References
232
CHAPTER
8
HILIC FOR FOOD, ENVIRONMENTAL,
AND OTHER APPLICATIONS
239
Michael A. Koupparis, Nikolaos C. Megoulas,
and Aikaterini M. Gremilogianni
8.1
Introduction
239
8.2
Food Applications for HILIC
240
X
CONTENTS
8.2.1 Review
of HILIC Analytical Methods
for Food Analysis
240
8.2.1.1
Sample Preparation in HILIC
Methods Applied to Food Matrices
240
8.2.1.2
HILIC Methods Applied to Food
Matrices:
Chromatographie
Parameters and Detection
242
8.2.2
Selected Detailed Examples of HILIC
Applications in Food Analysis
243
8.2.2.1
M
elamine
(MEL) and
Су
anurie
Acid (CYA)
243
8.2.2.2
Water-Soluble
Vitamins
250
8.2.2.3
Seafood and Other Toxins
253
8.3
Environmental and Other Applications of HILIC
254
8.3.1
Review of Environmental Applications
Based on the Stages of Method
Development
254
8.3.2
Selected Detailed Examples of
Environmental and Other HILIC
Applications
256
8.3.2.1
Metals and Their Related Organic
Compounds
256
8.3.2.2
Pharmaceutical Compounds in
Aqueous Environmental Samples
256
8.3.2.3
Other Applications
257
8.4
Conclusions
257
References
259
CHAPTER
9
THEORY AND PRACTICE OF
TWO-DIMENSIONAL LIQUID
CHROMATOGRAPHY SEPARATIONS
INVOLVING THE HILIC MODE
OF SEPARATION
265
Stephen R. Groskreutz and Dwight R. Stoll
9.1
Fundamentals of Multidimensional Liquid
Chromatography
265
9.1.1
Scope
265
9.1.2
Potential Advantages of 2D Separations
over Conventional Separations
266
9.1.3
Modes of 2D Separation
269
9.1.3.1
Offline Fraction Transfer
270
9.1.3.2
Online Fraction Transfer
271
CONTENTS Xl
9.1.3.3
Conceptual
Comparison of
Different 2D Separation Modes
271
9.1.4
Undersampling
274
9.1.5
Orthogonality
276
9.2
Complementarity of HILIC Selectivity
to Other Separation Modes
278
9.3
Instrumentation and Experimental Considerations
278
9.3.1
Online versus Offline 2DLC
280
9.3.1.1
Offline 2DLC
280
9.3.1.2
Online 2DLC
282
9.3.2
Solvent Incompatibility
287
9.3.2.1
Partial Mobile Phase Evaporation
287
9.3.2.2
Fraction Transfer Volume Relative
to the Second Dimension Column
Volume
288
9.3.2.3
On-Column Focusing
288
9.3.3
Fast Separations
289
9.3.3.1
General Considerations for Fast
LC Separations
290
9.3.3.2
Fast HILIC Separations
290
9.4
Applications
291
9.4.1
TCM
291
9.4.2
Polymers
296
9.4.3
Oligonucleotides
296
9.5
The Future of HILIC Separations in 2DLC
298
References
298
INDEX
307
mpounds
il aîrîva
n ä
1
y t ícal
rrîiétlioi-CÏ
f ,
KýuřOipH
ilïc£
í n
сийга
ctrïoïr cKïro
írt a tögira
рЌу-^Х
I~Iţ
1-ї
G
)
Jí
;
separate
jpolaBtórnpóunds« thatr^lörtenjdifficültitöl
analyze!
formanééit
liquid
f chrornatographýjtHPLGJtÄlotheríanaly
ticala
IméthodsťOffeŕing
alcömprehensive reviév^thisbpok
enables readers
tód
funderstanding of
howíHILIGíwÓrks^hdthen!applýfthati
knowledge; to develop and implement?!
щущегу^огргасйсагаррисапмвї^щ
fHydrophiliCilnteracUonl
Chrotnatography
tbeginsiwitmdisciissmnsíoj&HILIGfretention
mecna^ţ
fnismś
Џ
statióriá^yl
priasèslfantl
;
general t
íncthodj
developmentřírhisísetsiAeífóuhdationíforthe*!
|bÖokVëxtènsiyè
coverage:
ó^applicationšiiTho
fcĽíi-
-^-i^:.-!,-dL.-.:_--__L-_^i
-ì.-
—
-—..^-ii
b--J:bj^hemj^l|app]i^nonsí^oreoyeí; there;isí
B
les
to
Snethods
ft^thefs^
ni
te
/iŔed ítof
ahalyźei
and
;
beiter|understand
;
theloompösitíoni
oft- pharmaceuticalll
biochèmicaÎlchemicalifo^iandinvir^
Imchemistr^mamifáciňiririgfandcont^
|the
development
and[support óf
more
:
than twenty-five
ćónmierdalized
as numerous |
pdevelopmental
drugsMíFellowřóf
the Amencan Aşşociatioh
óf
Pharmaceutical
Scientistsj
Ör.
01sen||
feírrentlyiíprovides-
consultingíänd í
training
í
services
to j
the« pharmaceutical
;
industry; and seryes;
asj
.„. .-.,-, ...
liGommitţee
^^Ön
Monograph
^^
Development:
Small Molecules
3í*
** **
Slth^idèvélopméhtJófimany^lölidlöraïfan^fparentérall
»èïforms^lHelisfrecognizédlfprlhi^contrib^
¡IhaterialrCÓhtröí^trátegÍesí
genq^
РасЩ
ihašf
published
і
ohi theì
topicšV
óf
s HILIG?
äpplicatioriší
Ramahł
speetrosčopýjfrnass íspectroŕnetry. t
idissolutioní ähddeahíngivérificátióníi
|
| any_adam_object | 1 |
| author2 | Olsen, Bernard A. |
| author2_role | edt |
| author2_variant | b a o ba bao |
| author_facet | Olsen, Bernard A. |
| building | Verbundindex |
| bvnumber | BV040899654 |
| classification_rvk | VG 7500 WC 3480 |
| ctrlnum | (OCoLC)840765310 (DE-599)OBVAC10724337 |
| dewey-full | 543.84 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 543 - Analytical chemistry |
| dewey-raw | 543.84 |
| dewey-search | 543.84 |
| dewey-sort | 3543.84 |
| dewey-tens | 540 - Chemistry and allied sciences |
| discipline | Chemie / Pharmazie Biologie |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01769nam a2200385 cb4500</leader><controlfield tag="001">BV040899654</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20130507 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">130320s2013 ad|| |||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781118054178</subfield><subfield code="c">(hbk.) £66.95</subfield><subfield code="9">978-1-118-05417-8</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)840765310</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)OBVAC10724337</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-11</subfield><subfield code="a">DE-355</subfield><subfield code="a">DE-19</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">543.84</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">VG 7500</subfield><subfield code="0">(DE-625)147206:253</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 3480</subfield><subfield code="0">(DE-625)148087:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Hydrophilic interaction chromatography</subfield><subfield code="b">a guide for practitioners</subfield><subfield code="c">ed. by Bernard A. Olsen ...</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Hoboken, N.J.</subfield><subfield code="b">Wiley</subfield><subfield code="c">2013</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">XVI, 313 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">Chemical analysis</subfield><subfield code="v">177</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Hydrophile Interaktions-Flüssigchromatographie</subfield><subfield code="0">(DE-588)7863259-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="653" ind1=" " ind2=" "><subfield code="a">Hydrophilic interaction liquid chromatography.</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Hydrophile Interaktions-Flüssigchromatographie</subfield><subfield code="0">(DE-588)7863259-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Olsen, Bernard A.</subfield><subfield code="4">edt</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">Chemical analysis</subfield><subfield code="v">177</subfield><subfield code="w">(DE-604)BV000008780</subfield><subfield code="9">177</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">Digitalisierung UB Regensburg</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025879162&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">Digitalisierung UB Regensburg</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025879162&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Klappentext</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-025879162</subfield></datafield></record></collection> |
| id | DE-604.BV040899654 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T00:34:54Z |
| institution | BVB |
| isbn | 9781118054178 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-025879162 |
| oclc_num | 840765310 |
| open_access_boolean | |
| owner | DE-11 DE-355 DE-BY-UBR DE-19 DE-BY-UBM |
| owner_facet | DE-11 DE-355 DE-BY-UBR DE-19 DE-BY-UBM |
| physical | XVI, 313 S. Ill., graph. Darst. |
| publishDate | 2013 |
| publishDateSearch | 2013 |
| publishDateSort | 2013 |
| publisher | Wiley |
| record_format | marc |
| series | Chemical analysis |
| series2 | Chemical analysis |
| spelling | Hydrophilic interaction chromatography a guide for practitioners ed. by Bernard A. Olsen ... Hoboken, N.J. Wiley 2013 XVI, 313 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Chemical analysis 177 Hydrophile Interaktions-Flüssigchromatographie (DE-588)7863259-6 gnd rswk-swf Hydrophilic interaction liquid chromatography. Hydrophile Interaktions-Flüssigchromatographie (DE-588)7863259-6 s DE-604 Olsen, Bernard A. edt Chemical analysis 177 (DE-604)BV000008780 177 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025879162&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025879162&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Klappentext |
| spellingShingle | Hydrophilic interaction chromatography a guide for practitioners Chemical analysis Hydrophile Interaktions-Flüssigchromatographie (DE-588)7863259-6 gnd |
| subject_GND | (DE-588)7863259-6 |
| title | Hydrophilic interaction chromatography a guide for practitioners |
| title_auth | Hydrophilic interaction chromatography a guide for practitioners |
| title_exact_search | Hydrophilic interaction chromatography a guide for practitioners |
| title_full | Hydrophilic interaction chromatography a guide for practitioners ed. by Bernard A. Olsen ... |
| title_fullStr | Hydrophilic interaction chromatography a guide for practitioners ed. by Bernard A. Olsen ... |
| title_full_unstemmed | Hydrophilic interaction chromatography a guide for practitioners ed. by Bernard A. Olsen ... |
| title_short | Hydrophilic interaction chromatography |
| title_sort | hydrophilic interaction chromatography a guide for practitioners |
| title_sub | a guide for practitioners |
| topic | Hydrophile Interaktions-Flüssigchromatographie (DE-588)7863259-6 gnd |
| topic_facet | Hydrophile Interaktions-Flüssigchromatographie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025879162&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025879162&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV000008780 |
| work_keys_str_mv | AT olsenbernarda hydrophilicinteractionchromatographyaguideforpractitioners |