Metabolomics in practice: successful strategies to generate and analyze metabolic data
Gespeichert in:
| Weitere Verfasser: | |
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| Format: | Buch |
| Sprache: | English |
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Weinheim
Wiley-VCH
2013
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| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XXV, 415 S. Ill., graph. Darst. |
| ISBN: | 9783527330898 9783527655861 |
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| 245 | 1 | 0 | |a Metabolomics in practice |b successful strategies to generate and analyze metabolic data |c ed. by Michael Lämmerhofer ... |
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| adam_text | IMAGE 1
CONTENTS
LIST O F CONTRIBUTORS X V PREFACE XXIII
1 THE SAMPLING AND SAMPLE PREPARATION PROBLEM IN MICROBIAL METABOLOMICS
1 WALTER M. VAN GULIK, ANDR6 B. CANELAS, REZA M. SEIFAR, AND JOSEPH J.
HEIJNEN 1.1 INTRODUCTION 1
1.2 MICROORGANISMS AND THEIR PROPERTIES 1
1.3 SAMPLING METHODS 2
1.3.1 THE NEED FOR RAPID SAMPLING 2
1.3.2 SAMPLING SYSTEMS 3
1.4 QUENCHING 4
1.4.1 QUENCHING PROCEDURES AND THEIR PROPERTIES 4 1.4.2 VALIDATION O F
THE QUENCHING PROCEDURE AND MINIMIZATION OF METABOLITE LEAKAGE 5 1.4.3
QUENCHING PROCEDURE FOR DETERMINATION O F INTRACELLULAR METABOLITES
IN THE PRESENCE O F EXTRACELLULAR ABUNDANCE 6 1.4.4 QUENCHING O F
BACTERIA 7
1.5 METABOLITE EXTRACTION 9
1.5.1 EXTRACTION METHODS AND THEIR PROPERTIES 9 1.5.2 VALIDATION O F
EXTRACTION METHODS FOR YEAST METABOLOMICS 9 1.6 APPLICATION O F 13
C-LABELED INTERNAL STANDARDS 13 1.7 CONCLUSIONS 17
REFERENCES 18
2 TANDEM MASS SPECTROMETRY HYPHENATED WITH HPLC AND UHPLC FOR TARGETED
METABOLOMICS 21 GERARD HOPFGARTNER AND EMMANUEL VARESIO 2.1 INTRODUCTION
21
2.2 LC-MS-BASED TARGETED METABOLOMICS 22 2.3 LIQUID CHROMATOGRAPHY 22
HTTP://D-NB.INFO/102218136X
IMAGE 2
V I I CONTENTS
2.4 MASS SPECTROMETRY 27
2.4.1 IONIZATION TECHNIQUES 27
2.4.2 MASS ANALYZERS 28
2.5 SAMPLE PREPARATION 30
2.6 RELATIVE AND ABSOLUTE QUANTIFICATION 31 2.7 APPLICATIONS 32
2.8 SYNOPSIS 34
REFERENCES 35
3 UNCERTAINTY O F MEASUREMENT IN QUANTITATIVE METABOLOMICS 39 RAJFAELE
GUERRASIO, CHRISTINA HABERHAUER-TROYER, STEFAN NEUBAUER, KRISTAPS
KLAVINS, MADELEINE WEMETH, GUNDA KOELLENSPERGER, AND STEPHEN H A N N 3.1
INTRODUCTION 39
3.1.1 MS-BASED TECHNIQUES IN METABOLOMICS 39 3.1.2 UNCERTAINTY O F
MEASUREMENT IN QUANTITATIVE ANALYSIS 41 3.1.2.1 DEFINITION 41
3.1.2.2 UNCERTAINTY CALCULATION ACCORDING TO THE BOTTOM-UP APPROACH 42
3.2 UNCERTAINTIES O F QUANTITATIVE MS EXPERIMENTS 48 3.2.1 UNCERTAINTIES
I N SAMPLE PREPARATION 48 3.2.1.1 SAMPLING AND SAMPLE PREPARATION IN
METABOLITE PROFILING IN
FERMENTATIONS 49
3.2.1.2 CALCULATION O F SAMPLE PREPARATION UNCERTAINTY FOR INTRACELLULAR
METABOLITE QUANTITATION IN YEAST: A PRACTICAL EXAMPLE 53 3.2.1.3 LC-MS
59
3.2.2 UNCERTAINTY OF MASS SPECTROMETRIC ASSAYS (LC-MS AND GC-MS
MEASUREMENTS) 61 3.2.2.1 GC-MS 61
3.2.2.2 CALCULATION O F UNCERTAINTY FOR LC-MS MEASUREMENTS O F CELL
EXTRACTS: A PRACTICAL EXAMPLE 63 3.3 CONCLUDING REMARKS 66
ABBREVIATIONS 66 ACKNOWLEDGMENT 67 REFERENCES 67
4 GAS CHROMATOGRAPHY AND COMPREHENSIVE TWO-DIMENSIONAL GAS
CHROMATOGRAPHY HYPHENATED WITH MASS SPECTROMETRY FOR TARGETED AND
NONTARGETED METABOLOMICS 69 SONG YANG, JAMIN C. HOGGARD, MARY E.
LIDSTROM, AND
ROBERT E. SYNOVEC
4.1 INTRODUCTION AND SCOPE 69
4.2 SAMPLE PREPARATION FOR GC-BASED METABOLITE PROFILING 71 4.3 GC-MS
AND GC X GC-TOFMS INSTRUMENTATION FOR METABOLOMICS 74 4.4 DATA ANALYSIS
STRATEGIES AND SOFTWARE 82
IMAGE 3
CONTENTS I V I I
4.5 ILLUSTRATIVE EXAMPLES AND CONCLUDING REMARKS 88
REFERENCES 89
5 LC-MS-BASED NONTARGETED METABOLOMICS 93 GEORGIOS A. THEODORIDIS, HELEN
G. GIKA, AND IAN D. WILSON 5.1 INTRODUCTION 93
5.2 LC-MS-BASED UNTARGETED METABOLOMICS 94 5.2.1 LC ISSUES 94
5.2.2 MASS SPECTROMETRY 97
5.3 STUDY DESIGN 98
5.4 SAMPLE PREPARATION 100
5.5 ANALYTICAL STRATEGIES 103
5.6 DATA ANALYSIS 104
5.7 METABOLITE IDENTIFICATION 107
5.8 APPLICATIONS 109
5.9 SYNOPSIS 112
REFERENCES 113
6 THE POTENTIAL O F ULTRAHIGH RESOLUTION MS (FTICR-MS) IN METABOLOMICS
117 FRANCO MORITZ, SARA FORCISI, MOURAD HARIR, BASEM KANAWATI, MARIANNA
LUCIO, DIMITRIOS TZIOTIS, AND PHILIPPE SCHMITT-KOPPLIN 6.1 INTRODUCTION
117
6.2 METABOLOMICS TECHNOLOGIES 118
6.3 PRINCIPLES OF FTICR-MS 121
6.3.1 NATURAL ION MOVEMENT INSIDE AN ICR CELL SUBJECTED TO MAGNETIC AND
ELECTRIC FIELDS 121 6.3.2 APPLIED PHYSICAL TECHNIQUES IN FTICR-MS 123
6.3.3 PRACTICAL ADVANTAGES OF FTICR-MS 124
6.4 PROCEEDING IN METABOLOMICS 126
6.4.1 NETWORK ANALYSIS AND NETCALC COMPOSITION ASSIGNMENT 126 6.4.2
STATISTICS ON FTICR-MS DATASETS 127 6.5 APPLICATION EXAMPLE I N
METABOLOMICS USING FTICR-MS EXHALED BREATH CONDENSATE 128
6.5.1 THE EXPERIMENT 128
6.5.2 FT-ICR/MS MEASUREMENT 129 6.5.3 DATA PREPROCESSING 129
6.5.4 C - H - N - O - S - P FORMULA ANNOTATION 130
6.5.5 STATISTICAL ANALYSIS 130
6.5.5.1 STATISTICAL PREPROCESSING 130 6.5.6 SYNTHESIS O F BIOCHEMICAL
MASS DIFFERENCE NETWORKING AND STATISTICAL RESULTS 131 6.6 CONCLUSION
AND REMARKS 134
REFERENCES 134
IMAGE 4
V I I I | CONTENTS
7 THE ART AND PRACTICE O F LIPIDOMICS 137
KOEN SANDRA, RUBEN T KINDT, LUCIE JORGE, AND PAT SANDRA ABBREVIATIONS
137 7.1 INTRODUCTION 139
7.2 LIPID DIVERSITY 140
7.3 TACKLING THE LIPIDOME: STATE-OF-THE-ART 141 7.4 LC-MS-BASED
LIPIDOMICS 146 7.4.1 LIPID EXTRACTION 146
7.4.1.1 BIOLOGICAL FLUIDS AND CELLULAR MATERIAL 146 7.4.1.2 SKIN
(STRATUM CORNEUM) 148 7.4.1.3 SOLID-PHASE EXTRACTION (SPE) 148 7.4.2
LC-MS(/MS) 151
7.4.2.1 RETENTION TIME CHARACTERISTICS 151 7.4.2.2 IONIZATION
CHARACTERISTICS 155 7.4.2.3 IDENTIFICATION O F LIPIDS 156 7.4.3 DATA
PROCESSING AND ANALYSIS 161
7.5 GC-MS-BASED LIPIDOMICS 165 7.5.1 SAMPLE PREPARATION 165
7.5.2 GC-MS 166
7.5.3 DATA PROCESSING AND ANALYSIS 170 7.6 CONCLUSION 172
REFERENCES 173
8 THE ROLE O F CE-MS IN METABOLOMICS 277
RAWI RAMAUTAR, GOVERT W. SOMSEN, AND GERHARDUS J. DE JONG ABBREVIATIONS
177 8.1 INTRODUCTION 177
8.2 CE-MS 179
8.2.1 CE SEPARATION CONDITIONS 179 8.2.2 CE-MS COUPLING 180
8.2.2.1 INTERFACING 180
8.2.2.2 MASS ANALYZERS 184 8.3 SAMPLE PRETREATMENT 185
8.4 DATA ANALYSIS 187
8.5 APPLICATIONS 190
8.5.1 TARGETED APPROACHES 190
8.5.2 NONTARGETED APPROACHES 202 8.6 CONCLUSIONS AND PERSPECTIVES 203
REFERENCES 206
9 NMR-BASED METABOLOMICS ANALYSIS. 209 ANDREA LUBBE, KASHIFALI, ROBERT
VERPOORTE, AND YOUNG HAE CHOI 9.1 INTRODUCTION 209
9.2 PLATFORMS FOR METABOLOMICS 210
9.2.1 MASS SPECTROMETRY (MS) 210
IMAGE 5
CONTENTS I I X
9.2.1.1 GAS CHROMATOGRAPHY-MASS SPECTROMETRY (GC-MS) 210
9.2.1.2 LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY (LC-MS) 211 9.2.1.3
CAPILLARY ELECTROPHORESIS-MASS SPECTROMETRY (CE-MS) 211 9.2.1.4 FOURIER
TRANSFORM-ION. CYCLOTRON RESONANCE-MASS SPECTROMETRY
(FT-ICR-MS) 212
9.2.2 FOURIER TRANSFORM-INFRARED SPECTROSCOPY (FT-IR) 212 9.2.3 NUCLEAR
MAGNETIC RESONANCE SPECTROSCOPY: PRINCIPLES AND TECHNIQUES 212 9.2.3.1
ONE-DIMENSIONAL NUCLEAR MAGNETIC RESONANCE ( J H AND 13 C
NMR) 213
9.2.3.2 J-RESOLVED SPECTROSCOPY (JRES) 215 9.2.3.3 CORRELATION
SPECTROSCOPY (COSY) 215 9.2.3.4 TOTAL CORRELATION SPECTROSCOPY (TOCSY)
217 9.2.3.5 HETERONUCLEAR TWO-DIMENSIONAL METHODS 217 9.2.3.6 COMBINED
TWO-DIMENSIONAL METHODS 217 9.3 NMR FOR METABOLOMICS 219
9.3.1 SAMPLE PREPARATION 220
9.3.2 METABOLITE IDENTIFICATION 221 9.3.3 DATA ANALYSIS: TURNING DATA
INTO INFORMATION, POSSIBLY KNOWLEDGE 223 9.3.3.1 DATA PREPROCESSING 223
9.3.3.2 PRINCIPAL COMPONENT ANALYSIS (PCA) 225 9.3.3.3 PARTIAL LEAST
SQUARES (PLS) PROJECTIONS TO LATENT STRUCTURES 226 9.3.3.4 BIDIRECTIONAL
ORTHOGONAL-PLS (02PLS) 227 9.3.3.5 VALIDATION 227
9.4 APPLICATIONS O F NMR-BASED METABOLOMICS 228 9.4.1 UNDERSTANDING
STRESS RESPONSE 228 9.4.2 APPLICATION TO BIOACTIVITY SCREENING 230 9.4.3
QUALITY CONTROL O F HERBAL MEDICINES 231 9.4.4 CHEMOTAXONOMY 232
9.4.5 AGRICULTURAL APPLICATIONS 233 9.5 FUTURE PROSPECTS AND CONCLUSIONS
233
REFERENCES 234
10 POTENTIAL O F MICROFLUIDICS AND SINGLE CELL ANALYSIS IN METABOLOMICS
(MICROMETABOLOMICS) 239 MEGHAN M. MENSACK, RYAN E. HOLCOMB, AND CHARLES
S. HENRY 10.1 INTRODUCTION 239
10.2 SAMPLE PROCESSING FOR METABOLOMICS 240 10.2.1 SOLID PHASE
EXTRACTION 240 10.2.2 LAMINAR DIFFUSION 241 10.2.3 FLUIDIC PUMPING FOR
ON-CHIP MIXING 242
10.3 MICROFLUIDIC SEPARATIONS FOR METABOLIC ANALYSIS 243
IMAGE 6
X I CONTENTS
10.3.1 MICROCHIP CAPILLARY ELECTROPHORESIS 243
10.3.1.1 MCE SYSTEMS 243 10.3.1.2 SAMPLE INJECTION 244 10.3.1.3
ELECTROPHORETIC SEPARATIONS 244 10.3.2 ANALYTE DETECTION 245 10.3.2.1
OPTICAL DETECTION 245 10.3.2.2 ELECTROCHEMICAL DETECTION 246 10.4
MICROFLUIDICS FOR CELLULAR ANALYSIS 247 10.4.1 REQUIREMENTS FOR SINGLE
CELL METABOLOMICS 247 10.4.2 TYPES O F MICROFLUIDIC INSTRUMENTATION 248
10.4.3 BIOLOGICAL QUESTIONS 249 10.4.3.1 MONITORING METABOLIC RESPONSE
TO STIMULATION AND CELL-TO-CELL
SIGNALING 249
10.4.3.2 PHARMACOKINETICS/PHARMACODYNAMICS 252 10.4.3.3 CLINICAL
DIAGNOSTICS 254 10.5 A LOOK FORWARD 254
REFERENCES 256
11 DATA PROCESSING IN METABOIOMICS 261
AGE K. SMILDE, MARGRIET M.W.B. HENDRIKS, JOHAN A. WESTERHUIS, AND HUUB
C.J. HOEFSLOOT 11.1 INTRODUCTION AND SCOPE 261
11.2 CHARACTERISTICS O F METABOLOMICS DATA 261 11.2.1 CORRELATION
STRUCTURE O F METABOLOMICS DATA 261 11.2.2 INFORMATIVE VERSUS
NONINFORMATIVE VARIATION 262 11.2.3 LOW SAMPLES-TO-VARIABLES RATIO 263
11.2.4 MEASUREMENT ERROR 263
11.2.5 DYNAMICS 263
11.2.6 NONLINEAR RELATIONS 264 11.3 TYPES O F BIOLOGICAL QUESTIONS ASKED
264 11.3.1 METHODS SHOULD FOLLOW THE QUESTIONS 264 11.3.2 BIOMARKERS 264
11.3.3 TREATMENT EFFECTS 264 11.3.4 NETWORKS AND MECHANISTIC INSIGHT 265
11.4 VALIDATION 265
11.4.1 SEVERAL LEVELS O F VALIDATION 265 11.4.2 CURSE O F DIMENSIONALITY
266 11.4.3 CROSS-VALIDATION AND PERMUTATIONS 267 11.5 OVERVIEW O F
METHODS 272
11.5.1 EXPLORATORY ANALYSIS 272 11.5.2 ANOVA AND OTHER UNIVARIATE
METHODS 273 11.5.3 ADVANCED EXPLORATORY ANALYSIS 275 11.5.4 REGRESSION
METHODS 277 11.5.5 DISCRIMINANT ANALYSIS 280 11.5.6 MULTILEVEL
APPROACHES 281
IMAGE 7
CONTENTS | X I
11.5.7 NETWORK INFERENCE 282
REFERENCES 283
12 METABOLIC FLUX ANALYSIS 285
CHRISTOPH WITTMANN AND JEAN-CHARLES PORTALS 12.1 INTRODUCTION 285
12.2 PREREQUISITES FOR FLUX STUDIES 287
12.2.1 NETWORK TOPOLOGY AND CELLULAR COMPOSITION 287 12.2.2 NETWORK
FORMULATION AND CONDENSATION 287 12.2.3 METABOLIC AND ISOTOPIC STEADY
STATE 288 12.2.4 DEFINITION O F ISOTOPE LABELING PATTERNS 289 12.3
STOICHIOMETRIC FLUX ANALYSIS 290 12.4 LABELING STUDIES USING ISOTOPES
292 12.4.1 RADIOLABELED ISOTOPES 293 12.4.2 STABLE ISOTOPES 295
12.5 STATE-OF-ART 13 C FLUX ANALYSIS 296 12.5.1 MODELING OF CARBON
TRANSITIONS 298 12.5.2 EXPERIMENTAL DESIGN 299
12.5.3 FLUX CALCULATION AND STATISTICAL EVALUATION O F FLUX DATA 300
12.5.4 LABELING ANALYSIS BY MASS SPECTROMETRY 301 12.5.5 LABELING
ANALYSIS BY NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY 302 12.6 APPLICATION
O F METABOLIC FLUX ANALYSIS 303 12.6.1 IMPROVEMENT O F INDUSTRIAL
PRODUCTION STRAINS 303 12.6.2 INTEGRATION INTO SYSTEMS BIOLOGY
APPROACHES 306
12.7 RECENT ADVANCES IN THE FIELD 307
12.7.1 HIGH-THROUGHPUT FLUX SCREENING 307 12.7.2 FLUX DYNAMICS 307
12.8 CONCLUDING REMARKS 308
ACKNOWLEDGMENTS 308 REFERENCES 308
13 METABOLOMICS: APPLICATION IN PLANT SCIENCES 313 GAITAN GLAUSER,
JULIEN BOCCARD, JEAN-LUC WOLFENDER, AND SERGE RUDAZ 13.1 INTRODUCTION
313
13.2 SAMPLE PREPARATION 314
13.2.1 CULTURE AND HARVESTING 314 13.2.2 STORAGE AND DRYING 315
13.2.3 EXTRACTION 315
13.3 ANALYTICAL METHODS 316
13.3.1 NMR-BASED METHODS 317 13.3.1.1 DIRECT NMR FINGERPRINTING 317
13.3.1.2 APPLICATIONS 318 13.3.1.3 HYPHENATION OF NMR TO SEPARATING
TECHNIQUES 323
13.3.1.4 FUTURE TRENDS 323 13.3.2 MS-BASED METHODS 323
IMAGE 8
X I I I CONTENTS
13.3.2.1 DIRECT MS METHODS 324
13.3.2.2 HYPHENATION O F MS TO SEPARATING TECHNIQUES 324 13.3.2.3
APPLICATIONS 326 13.3.3 COMBINED APPROACHES 329 13.4 METABOLITE
IDENTIFICATION 330
13.4.1 INTERPRETING MASS SPECTRA 330 13.4.2 DATABASES 332
13.5 STRUCTURAL ELUCIDATION O F NOVEL METABOLITES AND VALIDATION OF
MODEL 334 13.6 CONCLUSION AND PERSPECTIVES 336 ACKNOWLEDGMENTS 337
REFERENCES 337
14 METABOLOMICS AND ITS ROLE IN THE STUDY O F MAMMALIAN SYSTEMS 345
WARWICK B. DUNN, MAMAS MAMAS, AND ALEXANDER HEAZELL 14.1 INTRODUCTION -
FROM EARLY BEGINNINGS 345 14.2 HYPOTHESIS GENERATION OR
HYPOTHESIS-TESTING STUDIES 347
14.3 UNTARGETED, SEMI-TARGETED, AND TARGETED ANALYTICAL EXPERIMENTS 348
14.4 STUDY AND EXPERIMENTAL DESIGN 350 14.5 SAMPLE TYPES 355
14.6 QUALITY ASSURANCE AND QUALITY CONTROL 360 14.7 METABOLITE
ANNOTATION AND IDENTIFICATION 365 14.8 APPLICATIONS 369
14.8.1 PREGNANCY COMPLICATIONS 369 14.8.2 CARDIOVASCULAR DISEASES 371
ACKNOWLEDGMENTS 373 REFERENCES 373
15 METABOLOMICS IN BIOTECHNOLOGY (MICROBIAL METABOLOMICS) 379 MARCO
OLDIGES, STEPHAN NOACK, AND NICOLE PACZIA 15.1 INTRODUCTION 379
15.2 ANALYTICAL METHODS APPLIED FOR MICROBIAL METABOLOMICS 382 15.3
CUSTOM-MADE SEPARATION FOR MICROBIAL METABOLOMICS 384 15.4 MICROBIAL
METABOLOMICS WITH HIGHER THROUGHPUT 385 15.5 APPLICATION OF MICROBIAL
METABOLOMICS 386 15.6 CONCLUSION 388
REFERENCES 388
16 NUTRITIONAL METABOLOMICS 393
HANNELORE DANIEL
16.1 INTRODUCTION 393
16.2 THE METABOLOME O F H U M A N PLASMA AND URINE: GENERAL
CONSIDERATIONS 393 16.2.1 THE PLASMA METABOLOME 394
IMAGE 9
CONTENTS I X I I I
16.2.2 THE URINARY METABOLOME 395
16.3 THE FOOD METABOLOME AND ITS SIGNATURE IN H U M A N SAMPLES 396 16.4
THE VARIABILITY O F THE HUMAN METABOLOME IN HEALTH AND DISEASE STATES
398
16.5 THE DYNAMIC NATURE O F THE METABOLOME 400 16.6 THE FUTURE O F
NUTRITIONAL METABOLOMICS AND RESEARCH NEEDS 402 REFERENCES 403
INDEX 407
|
| any_adam_object | 1 |
| author2 | Lämmerhofer, Michael 1966- |
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| author_GND | (DE-588)102455936X |
| author_facet | Lämmerhofer, Michael 1966- |
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| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.36 |
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| genre | (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV040791505 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T00:33:56Z |
| institution | BVB |
| isbn | 9783527330898 9783527655861 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-025769629 |
| oclc_num | 828723379 |
| open_access_boolean | |
| owner | DE-11 DE-578 DE-29 DE-91G DE-BY-TUM DE-703 |
| owner_facet | DE-11 DE-578 DE-29 DE-91G DE-BY-TUM DE-703 |
| physical | XXV, 415 S. Ill., graph. Darst. |
| publishDate | 2013 |
| publishDateSearch | 2013 |
| publishDateSort | 2013 |
| publisher | Wiley-VCH |
| record_format | marc |
| spelling | Metabolomics in practice successful strategies to generate and analyze metabolic data ed. by Michael Lämmerhofer ... Weinheim Wiley-VCH 2013 XXV, 415 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Biochemische Analyse (DE-588)4255721-5 gnd rswk-swf Metabolom (DE-588)4807318-0 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Metabolom (DE-588)4807318-0 s Biochemische Analyse (DE-588)4255721-5 s DE-604 Lämmerhofer, Michael 1966- (DE-588)102455936X edt Erscheint auch als Online-Ausgabe, EPUB 978-3-527-65588-5 Erscheint auch als Online-Ausgabe, MOBI 978-3-527-65587-8 Erscheint auch als Online-Ausgabe, PDF 978-3-527-65589-2 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025769629&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Metabolomics in practice successful strategies to generate and analyze metabolic data Biochemische Analyse (DE-588)4255721-5 gnd Metabolom (DE-588)4807318-0 gnd |
| subject_GND | (DE-588)4255721-5 (DE-588)4807318-0 (DE-588)4143413-4 |
| title | Metabolomics in practice successful strategies to generate and analyze metabolic data |
| title_auth | Metabolomics in practice successful strategies to generate and analyze metabolic data |
| title_exact_search | Metabolomics in practice successful strategies to generate and analyze metabolic data |
| title_full | Metabolomics in practice successful strategies to generate and analyze metabolic data ed. by Michael Lämmerhofer ... |
| title_fullStr | Metabolomics in practice successful strategies to generate and analyze metabolic data ed. by Michael Lämmerhofer ... |
| title_full_unstemmed | Metabolomics in practice successful strategies to generate and analyze metabolic data ed. by Michael Lämmerhofer ... |
| title_short | Metabolomics in practice |
| title_sort | metabolomics in practice successful strategies to generate and analyze metabolic data |
| title_sub | successful strategies to generate and analyze metabolic data |
| topic | Biochemische Analyse (DE-588)4255721-5 gnd Metabolom (DE-588)4807318-0 gnd |
| topic_facet | Biochemische Analyse Metabolom Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025769629&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT lammerhofermichael metabolomicsinpracticesuccessfulstrategiestogenerateandanalyzemetabolicdata |