Immune homeostasis: methods and protocols
Gespeichert in:
Weitere Verfasser: | |
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Format: | Buch |
Sprache: | English |
Veröffentlicht: |
New York [u.a.]
Humana Press
2013
|
Schriftenreihe: | Methods in molecular biology
979 |
Online-Zugang: | Inhaltsverzeichnis |
Beschreibung: | XI, 215 S. Ill., graph. Darst. |
ISBN: | 9781627032896 |
Internformat
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Datensatz im Suchindex
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adam_text | Titel: Immune homeostasis
Autor: Snow, Andrew L
Jahr: 2013
Contents
Preface.......................................................... v
Contributors...................................................... ix
1 Quantitating Lymphocyte Programmed Cell Death In Vitro
Using Simple Kill Assays......................................... 1
Lixin Zheng
2 Fluorescence-Activated Cell Sorting-Based Quantitation
of T Cell Receptor Restimulation-Induced Cell Death in Activated,
Primary Human T Cells......................................... 15
Gil Katz and Andrew L. Snow
3 Evaluation of IL-2-Withdrawal-Induced Apoptosis
in Human T Lymphocytes....................................... 25
Joao Bosco Oliveira
4 Determination of Apoptosis Sensitivity in Specific T Cell Subsets
from Human Peripheral Blood by Utilizing a Multiparameter
Fluorescence-Activated Cell Sorting-Based Technique................... 33
Bernice Lo and Madbu Ramaswamy
5 Visualization of Fas-Mediated Death-Inducing Signaling
Complex Formation by Immunoprecipitation......................... 43
Nelia Cordeiro and Nicolas Bidère
6 Analyses of Programmed Cell Death in Dendritic Cells.................. 51
Min Chen, lily Huang, and Jin Wang
7 Detection of Necrosis by Release of Lactate Dehydrogenase Activity........ 65
Francis Ka-Ming Chan, Kenta Moriwaki, and MaHa José De Rosa
8 Assessment of CD4+ and CD8+ T Cell Responses
Using MHC Class I and II Tetramers............................... 71
Sema Kurtulus and David Hildeman
9 Homeostatic Proliferation of Mature T Cells.......................... 81
Christopher E. Martin, Kwesi Frimpong-Boateng, Darina S. Spasova,
John C. Stone, and Charles D. Surh
10 Quantitating Lymphocyte Homeostasis In Vivo in Humans
Using Stable Isotope Tracers...................................... 107
Liset Westera, Yan Zhang, Kiki Tesselaar, José A.M. Borghans,
and Derek C. Macallan
11 Real-Time Quantitative (RQ-)PCR Approach to Quantify
the Contribution of Proliferation to B Lymphocyte Homeostasis.......... 133
Menno C. van Zelm, Magdalena A. Berkowska, Mirjam van der Burg,
and Jacques J.M. van Dongen
vii
viii
Contents
12 Molecular Measurement of T Cell Receptor Excision Circles.............. 147
Heather E. Lynch and Gregory D. Sempowski
13 Isolation of RNA and the Synthesis and Amplification of cDNA
from Antigen-Specific T Cells for Genome-Wide Expression Analysis....... 161
R. Anthony Barnitz, Sabrina Imam, Kathleen Yates,
and W. Nicholas Haininjj
14 Designs for Massively Parallel Sequencing Approaches to Identify
Causal Mutations in Human Immune Disorders....................... 175
Tu Zhang and Helen C. Su
15 Flow Cytometric Measurement of SLAM-Associated Protein
and X-Linked Inhibitor of Apoptosis................................ 189
Rebecca A. Marsh, Jack J. Bleesing, and Alexandra H. Filipovich
16 In Vitro Suppression Assay for Functional Assessment
of Human Regulatory T Cells..................................... 199
DatQ. Tran
Index............................................................... 213
|
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illustrated | Illustrated |
indexdate | 2024-07-10T00:33:52Z |
institution | BVB |
isbn | 9781627032896 |
language | English |
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physical | XI, 215 S. Ill., graph. Darst. |
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series | Methods in molecular biology |
series2 | Methods in molecular biology Springer protocols |
spelling | Immune homeostasis methods and protocols ed. by Andrew L. ... New York [u.a.] Humana Press 2013 XI, 215 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 979 Springer protocols Snow, Andrew L. edt Methods in molecular biology 979 (DE-604)BV035362695 979 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025766935&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
spellingShingle | Immune homeostasis methods and protocols Methods in molecular biology |
title | Immune homeostasis methods and protocols |
title_auth | Immune homeostasis methods and protocols |
title_exact_search | Immune homeostasis methods and protocols |
title_full | Immune homeostasis methods and protocols ed. by Andrew L. ... |
title_fullStr | Immune homeostasis methods and protocols ed. by Andrew L. ... |
title_full_unstemmed | Immune homeostasis methods and protocols ed. by Andrew L. ... |
title_short | Immune homeostasis |
title_sort | immune homeostasis methods and protocols |
title_sub | methods and protocols |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025766935&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
volume_link | (DE-604)BV035362695 |
work_keys_str_mv | AT snowandrewl immunehomeostasismethodsandprotocols |