Highly fluorescent CdSe/CdS multidimensional hetero-nanostructures suitable for two-photon intestinal uptake tracking and nanoscopy:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Marburg
Görich und Weiershäuser
2012
|
| Schriftenreihe: | Wissenschaft in Dissertationen
895 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVI, 195 S. Ill., graph. Darst. |
| ISBN: | 9783897037830 |
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| adam_text | IMAGE 1
TABLE OF CONTENTS
TITLE ACKNOWLEDGMENTS V
ABBREVIATIONS VIII
ABSTRACT XII
TABLE O F CONTENTS XIV
1. INTRODUCTION 1
1.1 INTRODUCTION TO THE WORLD O F NEGLECTED DIMENSIONS 1
1.2 INTRODUCTION TO THE PROJECT BIO-NANO RESPONSE 3
1.2.1 RESULTS AND PROJECT REQUIREMENTS 3
1.2.2 AN INTRODUCTION TO TWO-PHOTON LASER SCANNING MICROSCOPY 3
1.2.3 ONE-PHOTON PROCESSES VS. TWO-PHOTON PROCESSES 5
1.3 IN-VIVO STUDY - INTRODUCTION TO THE SMALL INTESTINE EPITHELIUM 9
1.3.1 NOVEL MOUSE MODEL 11
1.4 LITERATURE 12
2. THEORETICAL BACKGROUND 19
2.1 INTRODUCTION TO THE THEORY O F BULK SOLIDS-SEMICONDUCTOR 19
2.1.1 BAND STRUCURES-DIRECT AND INDIRECT BAND-GAP MATERIALS 20 2.1.2
EFFECTIVE MASS 21
2.1.3 FORMATION O F AN EXCITON 21
2.2 INTRODUCTION TO THE THEORY O F SEMICONDUCTOR NANOCRYSTALS 23 2.2.1
SIZE QUANTIZATION EFFECT 23
2.2.2 CONFINEMENT REGIMES 25
2.2.3 BAND STRUCTURES 26
2.2.4 DENSITY O F THE STATES (DOS) 28
2.2.5 ELECTRONIC STUCTURES AND OPTICAL PROPERTIES O F QUANTUM DOTS AND
QUANTUM DOTS-IN-QUANTUM RODS 29
2.2.6 WAVE-FUNCTION ENGINEERING O F QUANTUM DOTS AND QUANTUM
DOTS-IN-QUANTUM RODS 32
2.3 SYNTHESIS O F SEMICONDUCTOR NANOCRYSTALS 37
2.3.1 INTRODUCTION TO NANOCRYSTAL SYNTHESIS 37
XIV
HTTP://D-NB.INFO/1029518629
IMAGE 2
2.3.1.1 SEEDED-GROWTH APPROACH 39
2.3.2 HOMOGENEOUS NUCLEATION-KINETICS AND THERMODYNAMICS 39 2.3.3
HETEROGENEOUS NUCLEATION 43
2.3.4 GROWTH AND SHAPE EVOLUTION O F NANOCRYSTALS 44
2.4 DETERMINATION O F PHOTO-LUMINESCENCE QUATUM YIELD 49
2.4.1 UV/VIS SPECTROSCOPY 49
2.4.2 FLUORESCENCE SPECTROSCOPY 53
2.4.3 INSTRUMENTATION FOR FLUORESCENCE SPECTROSCOPY 54
2.4.3.1 DISTORTION IN EMISSION AND EXCITATION SPECTRA 55
2.4.3.2 INSTRUMENTAL ERRORS 55
2.4.3.3 SAMPLE PREPARATION ERRORS 55
2.4.4 INTRODUCTION TO PHOTO-LUMINESCENCE QUANTUM YIELD AND ITS
DETERMINATION 56
2.5 LITERATURE 63
3. RESULTS AND DISCUSSIONS 69
3.1 SYNTHESIS O F SEEDED GROWTH HETERO-NANOSTRUCTURES 71
3.1.1 STRUCTURAL CHARACTERIZATION O F QUANTUM DOTS-IN-QUANTUM RODS 71
3.1.2 LINEAR OPTICAL PROPERTIES O F QUANTUM DOTS-IN-QUANTUM RODS 75
3.2 DETERMINATION O F RELATIVE AND ABSOLUTE PHOTO-LUMINESCENCE QUANTUM
YIELD O F QUANTUM DOTS-IN-QUANTUM RODS 77
3.2.1 FLUORESCENT QUANTUM YIELD STANDARDS AND INSTRUMETAL CHECK 77 3.2.2
THE COMPARATIVE METHOD FOR DETERMINING O F RELATIVE PHOTOLUMINESCENCE
QUANTUM YIELD 80
3.2.3 SPECIFIC SOURCES O F UNCERTAINTY IN DETERMINING PHOTO-LUMI
NESCENCE QUANTUM YIELD 82
3.3 ENCAPSULATION OF QUANTUM DOTS-IN-QUANTUM RODS IN MICELLE-LIKE
STRUCTURES WITH DI-BLOCK POLYMERS (PI-PEG) 85
3.3.1 ENCAPSULATION O F QDQRS IN CONTINUOUS FLOW SYSTEM 86
3.3.1.1 LIGAND ENCAPSULATION REACTION WITH PI 62 -B-PEG 193 -OH 86
3.3.1.2 CONCENTRATION AND STABILITY TEST IN WATER UPON DILUTION 90
3.3.1.3 BUFFER AND STABILITY TEST IN BUFFERS 92
3.3.1.4 CELL MEDIA TEST 94
3.3.1.5 LIGAND ENCAPSULATION METHOD WITH PI^-B-PEG^-NHJ AND PI 62 -B-PEG
214 -COOH 96
3.3.2 ENCAPSULATION OF QDQRS BY FAST HAND-INJECTION METHOD 97
X V
IMAGE 3
3.3.2.1 LIGAND ENCAPSULATION REACTION WITH PI 62 -B-PEG 193 -OH 97
3.3.2.2 LIGAND ENCAPSULATION METHOD WITH PI 62-B-PEGL93-NH2 AND PI 62
-B-PEG 214 -COOH 100
3.4 DETERMINATION O F CONCENTRATION O F CDSE QDS AND CDSE/CDS QDQRS 101
3.4.2 DETERMINATION O F THE MOLAR EXTINCTION COEFFICIENT 104
3.5 OPTICAL IMAGING TECHNIQUES FOR CHARACTERIZATION O F QDS AND QDQRS
107 3.5.1 CONFOCAL LASER SCANNING MICROSCOPY-LINEAR BEHAVIOUR 107 3.5.2
TWO-PHOTON LASER SCANNING MICROSOCPY-NON-LINEAR BEHVAIOUR 109 3.6 THE
APPLICATION OF HIGHLY FLUORESCENT CDSE/CDS QDQRS AND
CDSE/CDS/ZNS QDS 113
3.6.1 QDQRS AND QDS AS A TPLSM PROBE FOR AN IN-VIVO INVESTIGATI ON OF
THE SMALL INTESTINE 113
3.6.2 QDQRS AS MARKER IN NANOSCOPY: GROUND STATE DEPLETION MICROSCOPY
114
3.7 LITERATURE 117
4. EXPERIMENTAL SECTION 122
4.1 SYNTHESIS O F CD-BASED NANOCRYSTALLITES 123
4.1.1. SYNTHESIS O F SPHERICAL CORE/SHELL NANOPARTICLES 123
4.1.2 ONE-POT SYNTHESIS O F CORE/SHELL NANOCRYSTALS 126
4.2 SYNTHESIS O F SPHERICAL CORE/SHELL/SHELL NANOCRYSTALS 127
4.3 SYNTHESIS OF CDSE/CDS QUANTUM DOTS-IN-QUANTUM RODS 128
4.4 LIGAND EXCHANGE AND ADDITION REACTIONS 129
4.4.1 LIGAND ENCAPSULATION REACTION BY THE CONTINUOUS-FLOW SYSTEM 129
4.4.2 LIGAND ENCAPSULATION REACTION BY FAST HAND-INJECTION METHOD 131
4.5 DETERMINATION OF RELATIVE PHOTO-LUMINESCENCE QUANTUM YIELD BASED ON
COMPARATIVE METHOD 132
4.6 DETERMINATION O F RED- AND GREEN-EMITTING QDS AND
RED-AND-ORANGEEMITTING QDQRS CONCENTRATION 133
4.6.1 DETERMINATION O F THE MOLAR EXTINCTION COEFFICIENT 133
4.7 STABILITY O F WATER-DISPERSED QDQRS 134
4.7.1 CONCENTRATION, DILUTION AND STABILITY TEST IN WATER 134
4.7.2 BUFFER TEST 134
4.7.3 STABILITY TEST O F QDQR IN BUFFERS 135
4.7.4 CELL MEDIA AND STABILITY TESTS 135
XVI
IMAGE 4
4.8 TEM AND TEM STAINING INVESTIGATIONS 135
4.9 PREPARATION O F SAMPLES FOR CLSM AND TPLSM 136
4.10 LITERATURE 137
5. CHARACTERIZATION METHODS 139
5.1 CONFOCAL LASER SCANNING MICROSCOPY (CLSM) 141
5.2 TWO-PHOTON LASER SCANNING MICROSCOPY (TPLSM) 141
5.3 STIMULATED EMISSION DEPLETION AND GROUND STATE DEPLETION MICROSCOPY
(STED AND GSDM) 142
5.4 TRANSMISSION ELECTRON MICROSCOPY (TEM) AND HIGH-RESOLUTION
TRANSMISSION MICROSCOPY (HR-TEM) 142
5.5 POWDER X-RAY SPECTROSCOPY (XRD) 143
5.6 SMALL ANGLE X-RAY SPECTROSCOPY (SAXS) 144
5.7 UV/VIS SPECTROSCOPY 144
5.8 FLUORESCENCE SPECTROSCOPY 145
5.9 INDUCTIVELY-COUPLED PLASMA-OPTICAL EMISSION SPECTROSOCPY (ICP-OES)
149
5.10 INDUCTIVELY-COUPLED PLASMA-MASS SPECTROMETRY (ICP-MS) 149
5.11 DYNAMIC LIGHT SCATTERING (DLS) 150
5.12 LITERATURE 151
6. SUMMARY AND OUTLOOK 155
6. ZUSAMMENFASUNG UND AUSBLICK 159
APPENDIX 165
AI USED CHEMICALS, HAZARD SYMBOLS AND H-AND-P PHRASES 167
ALL LIST O F FIGURES 178
AIII LIST O F TABLES 184
AIV LIST O F SCHEMES 185
AV DAY-LIGHT AND UV-LIGHT IMAGES O F SAMPLES USED 186
CURRICULUM VITAE 189
LEBENSLAUF 193
DECLERATION O F OATH 196
XVII
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| author | Dimitrijevic, Jelena |
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| bvnumber | BV040786866 |
| ctrlnum | (OCoLC)864638527 (DE-599)DNB1029518629 |
| dewey-full | 540 541.28 |
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| dewey-ones | 540 - Chemistry and allied sciences 541 - Physical chemistry |
| dewey-raw | 540 541.28 |
| dewey-search | 540 541.28 |
| dewey-sort | 3540 |
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| discipline | Chemie / Pharmazie |
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| spelling | Dimitrijevic, Jelena Verfasser (DE-588)135464056 aut Highly fluorescent CdSe/CdS multidimensional hetero-nanostructures suitable for two-photon intestinal uptake tracking and nanoscopy Jelena Dimitrijevic Marburg Görich und Weiershäuser 2012 XVI, 195 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Wissenschaft in Dissertationen 895 Hamburg, Univ., Diss., 2012 Cadmiumselenid (DE-588)4147107-6 gnd rswk-swf Polymere (DE-588)4046699-1 gnd rswk-swf Hülle (DE-588)4804018-6 gnd rswk-swf Nanokristall (DE-588)7574326-7 gnd rswk-swf Fluoreszenzquantenausbeute (DE-588)4288322-2 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Cadmiumselenid (DE-588)4147107-6 s Nanokristall (DE-588)7574326-7 s Polymere (DE-588)4046699-1 s Hülle (DE-588)4804018-6 s Fluoreszenzquantenausbeute (DE-588)4288322-2 s DE-604 Wissenschaft in Dissertationen 895 (DE-604)BV009979353 895 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025765087&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Dimitrijevic, Jelena Highly fluorescent CdSe/CdS multidimensional hetero-nanostructures suitable for two-photon intestinal uptake tracking and nanoscopy Wissenschaft in Dissertationen Cadmiumselenid (DE-588)4147107-6 gnd Polymere (DE-588)4046699-1 gnd Hülle (DE-588)4804018-6 gnd Nanokristall (DE-588)7574326-7 gnd Fluoreszenzquantenausbeute (DE-588)4288322-2 gnd |
| subject_GND | (DE-588)4147107-6 (DE-588)4046699-1 (DE-588)4804018-6 (DE-588)7574326-7 (DE-588)4288322-2 (DE-588)4113937-9 |
| title | Highly fluorescent CdSe/CdS multidimensional hetero-nanostructures suitable for two-photon intestinal uptake tracking and nanoscopy |
| title_auth | Highly fluorescent CdSe/CdS multidimensional hetero-nanostructures suitable for two-photon intestinal uptake tracking and nanoscopy |
| title_exact_search | Highly fluorescent CdSe/CdS multidimensional hetero-nanostructures suitable for two-photon intestinal uptake tracking and nanoscopy |
| title_full | Highly fluorescent CdSe/CdS multidimensional hetero-nanostructures suitable for two-photon intestinal uptake tracking and nanoscopy Jelena Dimitrijevic |
| title_fullStr | Highly fluorescent CdSe/CdS multidimensional hetero-nanostructures suitable for two-photon intestinal uptake tracking and nanoscopy Jelena Dimitrijevic |
| title_full_unstemmed | Highly fluorescent CdSe/CdS multidimensional hetero-nanostructures suitable for two-photon intestinal uptake tracking and nanoscopy Jelena Dimitrijevic |
| title_short | Highly fluorescent CdSe/CdS multidimensional hetero-nanostructures suitable for two-photon intestinal uptake tracking and nanoscopy |
| title_sort | highly fluorescent cdse cds multidimensional hetero nanostructures suitable for two photon intestinal uptake tracking and nanoscopy |
| topic | Cadmiumselenid (DE-588)4147107-6 gnd Polymere (DE-588)4046699-1 gnd Hülle (DE-588)4804018-6 gnd Nanokristall (DE-588)7574326-7 gnd Fluoreszenzquantenausbeute (DE-588)4288322-2 gnd |
| topic_facet | Cadmiumselenid Polymere Hülle Nanokristall Fluoreszenzquantenausbeute Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025765087&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV009979353 |
| work_keys_str_mv | AT dimitrijevicjelena highlyfluorescentcdsecdsmultidimensionalheteronanostructuressuitablefortwophotonintestinaluptaketrackingandnanoscopy |