Neocortex expansion in the human and ferret: a cell biological and molecular analysis of neural progenitors
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | V, 135 S. Ill., graph. Darst. |
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| 245 | 1 | 0 | |a Neocortex expansion in the human and ferret |b a cell biological and molecular analysis of neural progenitors |c Simone Angelika Fietz |
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Datensatz im Suchindex
| _version_ | 1804150107835203584 |
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| adam_text | IMAGE 1
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C O N T E N T S
SUMMARY 1 I N T R O D U C T I O N
1.1 NEOCORTEX EXPANSION DURING EVOLUTION
1.2 DEVELOPMENT O F THE MAMMALIAN NEOCORTEX
1.2.1 FORMATION OF T H E NEURAL T U B E
1.2.2 DIFFERENTIATION OF T H E NEURAL T U B E
1.2.3 DIFFERENTIATION OF T H E MAMMALIAN TELENCEPHALON
1.2.4 RADIAL UNITS IN LISSENCEPHALIC VS. GYRENCEPHALIC CORTICES
1.3 CELL BIOLOGICAL CHARACTERISTICS OF NEURAL S T E M AND PROGENITOR
CELLS . . .
1.3.1 APICAL PROGENITORS
1.3.2 BASAL PROGENITORS
1.3.3 SVZ EXPANSION DURING NEOCORTEX EVOLUTION
1.3.4 T H E EPITHELIAL PROGENITOR HYPOTHESIS
1.4 AIMS OF THIS STUDY
1.4.1 CELL BIOLOGICAL CHARACTERIZATION OF OSVZ PROGENITORS
1.4.2 GENOMIC CHARACTERIZATION OF OSVZ PROGENITORS
2 R E S U L T S
2.1 CELL BIOLOGICAL CHARACTERIZATION OF OSVZ PROGENITORS
2.1.1 H U M A N OSVZ PROGENITORS EXHIBIT CHARACTERISTICS OF RADIAL GLIA
2.1.2 H U M A N OSVZ PROGENITORS LACK APICAL CELL POLARITY
I
HTTP://D-NB.INFO/103002474X
IMAGE 2
CONTENTS
2.1.3 H U M A N OSVZ PROGENITORS RETAIN A BASAL PROCESS THROUGH MITOSIS
. 32
2.1.4 H U M A N OSVZ PROGENITORS SHOW RANDOM CLEAVAGE PLANE ORIENTATION
34
2.1.5 OSVZ PROGENITORS EXIST IN THE DEVELOPING FERRET NEOCORTEX 36
2.1.6 INTERFERENCE WITH INTEGRIN FUNCTION REDUCES T H E POPULATION SIZE
OF
FERRET OSVZ PROGENITORS 42
2.2 GENOMIC CHARACTERIZATION OF OSVZ PROGENITORS 46
2.2.1 M R N A SEQUENCING OF GERMINAL ZONES AND C P OF T H E DEVELOPING
H U M A N AND MOUSE NEOCORTEX 46
2.2.2 COMPARISON OF GENE EXPRESSION LEVELS OF SELECTED GENES FOR
GERMINAL
ZONES AND C P OF THE DEVELOPING H U M A N AND MOUSE NEOCORTEX . . . 50
2.2.3 DIFFERENTIAL GENE EXPRESSION ANALYSIS OF GERMINAL ZONES IN THE DE
VELOPING H U M A N AND MOUSE NEOCORTEX 51
2.2.4 GENE CLUSTERS WITH SIMILAR EXPRESSION PATTERNS IN THE GERMINAL
ZONES OF T H E DEVELOPING H U M A N NEOCORTEX 55
3 D I S C U S S I O N 6 1
3.1 CELL BIOLOGICAL CHARACTERIZATION OF OSVZ PROGENITORS 62
3.1.1 H U M A N OSVZ PROGENITORS ARE DELANIINATED RGCS RETAINING A
CANON
ICAL EPITHELIAL FEATURE 62
3.1.2 OSVZ PROGENITORS EXIST IN T H E FERRET, A NON-PRIMATE DEVELOPING A
GYRENCEPHALIC CORTEX 64
3.1.3 BASAL PROCESS RETENTION ALLOWS INTEGRIN-MEDIATED SELF-RENEWAL OF
OSVZ PROGENITORS 65
3.1.4 CONSEQUENCES OF TWO CONSECUTIVE REPEATED ASYMMETRIC DIVISIONS
FOR NEURONAL O U T P U T 66
3.2 GENOMIC CHARACTERIZATION OF OSVZ PROGENITORS 68
3.2.1 BIOLOGICAL PATHWAYS CRITICAL FOR SELF-RENEWAL OF H U M A N NEURAL
PRO
GENITORS 69
3.2.2 GENES CHARACTERISTIC FOR H U M A N OSVZ PROGENITORS 70
II
IMAGE 3
CONTENTS
3.3 FUTURE PERSPECTIVES 72
4 M A T E R I A L S A N D M E T H O D S 7 5
4.1 MATERIALS 76
4.1.1 TISSUE 76
4.1.2 EQUIPMENT 77
4.1.3 CHEMICALS AND REAGENTS 78
4.1.4 COMPUTER APPLICATIONS 83
4.2 METHODS 84
4.2.1 ORGANOTYPIC FERRET CORTICAL SLICE CULTURE 84
4.2.2 IMMUNOCYTOCHEMISTRY 84
4.2.3 DIL LABELING 85
4.2.4 I N SITU HYBRIDIZATION 85
4.2.5 ELECTRON MICROSCOPY 86
4.2.6 IMAGE ACQUISITION AND QUANTIFICATIONS 86
4.2.7 LASERCAPTURE MICRODISSECTION 88
4.2.8 R N A EXTRACTION AND CDNA LIBRARY PREPARATION 88
4.2.9 M R N A SEQUENCING 89
4.2.10 STATISTICAL ANALYSIS OF MRNA-SEQ D A T A 90
A B B R E V I A T I O N S 9 3
BIBLIOGRAPHY 9 5
P U B L I C A T I O N S 1 0 7
A C K N O W L E D G E M E N T S 1 0 9
DECLARATION 1 1 1
A S U P P L E M E N T A L T A B L E S 1 1 3
III
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| author | Fietz, Simone Angelika 1978- |
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| spelling | Fietz, Simone Angelika 1978- Verfasser (DE-588)1030024251 aut Neocortex expansion in the human and ferret a cell biological and molecular analysis of neural progenitors Simone Angelika Fietz 2012 V, 135 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Dresden, Techn. Univ., Diss., 2012 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025754580&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Fietz, Simone Angelika 1978- Neocortex expansion in the human and ferret a cell biological and molecular analysis of neural progenitors |
| subject_GND | (DE-588)4113937-9 |
| title | Neocortex expansion in the human and ferret a cell biological and molecular analysis of neural progenitors |
| title_auth | Neocortex expansion in the human and ferret a cell biological and molecular analysis of neural progenitors |
| title_exact_search | Neocortex expansion in the human and ferret a cell biological and molecular analysis of neural progenitors |
| title_full | Neocortex expansion in the human and ferret a cell biological and molecular analysis of neural progenitors Simone Angelika Fietz |
| title_fullStr | Neocortex expansion in the human and ferret a cell biological and molecular analysis of neural progenitors Simone Angelika Fietz |
| title_full_unstemmed | Neocortex expansion in the human and ferret a cell biological and molecular analysis of neural progenitors Simone Angelika Fietz |
| title_short | Neocortex expansion in the human and ferret |
| title_sort | neocortex expansion in the human and ferret a cell biological and molecular analysis of neural progenitors |
| title_sub | a cell biological and molecular analysis of neural progenitors |
| topic_facet | Hochschulschrift |
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