Neurogenesis from parthenogenetic human embryonic stem cells:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | Volltext Inhaltsverzeichnis |
| Item Description: | Zsfassung in dt. und engl. Sprache. - Online erschienen: 2013 |
| Physical Description: | 95 Bl. Ill., graph. Darst. |
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Record in the Search Index
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| adam_text | IMAGE 1
3
TABLE OF CONTENT
1 SUMMARY 6
2 ZUSAMMENFASSUNG 8
3 ABBREVIATIONS 10
4 INTRODUCTION 12
4.1 STEM CELLS: FEATURES AND FUNCTIONS 12
4.1.1 HUMAN EMBRYONIC STEM CELLS (HESCS) 13
4.1.2 NUCLEAR REPROGRAMMING 15
4.1.3 GERMLINE STEM CELLS 16
4.2 PLURIPOTENT UNIPARENTAL STEM CELLS: GENERATION AND DEVELOPMENTAL
POTENTIAL 17
4.2.1 ANDROGENETIC ESCS 17
4.2.2 PARTHENOGENETIC ESCS 17
4.2.3 DEVELOPMENTAL CAPABILITIES OF UNIPARENTAL CELLS 19
4.2.4 HPESCS: FEATURES AND PROSPECTIVE 20
4.3 NEURAL STEM CELLS: PROPERTIES AND DERIVATION FROM NUMEROUS SOURCES
22
4.4 IMPRINTING: MECHANISM AND INFLUENCE 24
4.4.1 ROLE OF IMPRINTING IN THE BRAIN 25
4.5 AIM AND STRATEGY 26
5 RESULTS 27
5.1 OPTIMIZATION OF HPESCS CULTURE CONDITIONS 27
5.2 HPESC-DERIVED TERATOMA IN NOD-SCID MICE 28
5.3 NEURAL DIFFERENTIATION OF HPESCS 30
5.4 EXPRESSION OF MITOTIC CHECKPOINT AND EXTRACELLULAR MATRIX GENE IN
HPESCS 34
5.5 TERMINAL DIFFERENTIATION OF HPESCS-DERIVED HPNSCS 36
5.6 REGION-SPECIFIC DIFFERENTIATION OF HPNSCS 38
5.7 ELECTROPHYSIOLOGICAL ANALYSIS OF HPESCS-DERIVED NEURONS 41
5.8 ANALYSES OF DIFFERENTIALLY METHYLATED REGIONS OF IMPRINTED BRAIN
GENES BY
BISULFITE SEQUENCING 43
5.9 ANALYSIS OF IMPRINTED BRAIN GENES BY QUANTITATIVE REAL-TIME PCR 45
6 DISCUSSION 48
6.1 MAINTENANCE OF HPESCS CULTURES 48
HTTP://D-NB.INFO/1033289124
IMAGE 2
4
6.2 NEURAL DIFFERENTIATION OF HPESCS 48
6.3 HPNSC-DERIVED NEURAL SUBTYPES 49
6.4 IMPRINTING IN HPESCS AND HPNSCS 50
6.5 PLURIPOTENT STEM CELLS 52
6.6 CONCLUSION 56
7 MATERIAL AND METHODS 57
7.1 MATERIAL 57
7.1.1 CELL LINES 57
7.1.2 ANTIBODIES 57
7.1.2.1 PRIMARY ANTIBODIES 57
7.1.2.2 SECONDARY ANTIBODIES 58
7.1.3 CELL CULTURE-RELATED MATERIALS 58
7.1.3.1 MEDIA AND SUPPLEMENTS 58
7.1.3.2 GROWTH FACTORS 59
7.1.3.3 MEDIUM FOR HPESCS CULTURE 59
7.1.3.4 MEDIUM FOR HPESC-DERIVED EMBRYOID BODIES CULTURE 59
7.1.4 CHEMICALS 60
7.1.5 CONSUMABLES 61
7.1.6 INSTRUMENTS 61
7.1.7 BUFFER AND SOLUTIONS 62
7.2 METHODS 62
7.2.1 HUMAN FORESKIN FIBROBLAST CELL CULTURE (HFF) 62
7.2.2 HPESC CULTURE 63
7.2.3 FREEZING OF HFFS AND HPESCS 63
7.2.4 THAWING OF HPESCS AND HFFS FROM LIQUID NITROGEN STORAGE 63
7.2.5 NEURAL IN VITRO DIFFERENTIATION 63
7.2.6 IMMUNOSTAINING OF CULTURED CELLS 64
7.2.7 BRAIN SAMPLE PREPARATION AND IMMUNOHISTOCHEMISTRY OF CRYOSECTIONS
65
7.2.8 RNA ISOLATION 66
7.2.9 DNASE TREATMENT 67
7.2.10 FIRST STRAND SYNTHESIS 67
IMAGE 3
5
7.2.11 ANALYSIS OF GENE EXPRESSION USING SEMI-QUANTITATIVE RT-PCR 67
7.2.12 ANALYSIS OF IMPRINTED BRAIN GENE EXPRESSION USING QUANTITATIVE
RT-PCR 68
8 REFRENCES 70
9 ACKNOWLEDGMENTS 90
10 AFFIDAVIT 91
11 CURRICULUM VITAE 93
12 PUBLICATIONS 93
|
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| spelling | Ahmad, Ruhel Verfasser (DE-588)1031431144 aut Neurogenesis from parthenogenetic human embryonic stem cells von Ruhel Ahmad Neurogenese von parthenogenetischen humanen embryonalen Stammzellen 2012 95 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. und engl. Sprache. - Online erschienen: 2013 Würzburg, Univ., Diss., 2012 Zelldifferenzierung (DE-588)4067536-1 gnd rswk-swf Neurogenese (DE-588)4331352-8 gnd rswk-swf Embryonale Stammzelle (DE-588)4699648-5 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Embryonale Stammzelle (DE-588)4699648-5 s Neurogenese (DE-588)4331352-8 s Zelldifferenzierung (DE-588)4067536-1 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:20-opus-75935 https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-75935 Resolving-System kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025739832&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Ahmad, Ruhel Neurogenesis from parthenogenetic human embryonic stem cells Zelldifferenzierung (DE-588)4067536-1 gnd Neurogenese (DE-588)4331352-8 gnd Embryonale Stammzelle (DE-588)4699648-5 gnd |
| subject_GND | (DE-588)4067536-1 (DE-588)4331352-8 (DE-588)4699648-5 (DE-588)4113937-9 |
| title | Neurogenesis from parthenogenetic human embryonic stem cells |
| title_alt | Neurogenese von parthenogenetischen humanen embryonalen Stammzellen |
| title_auth | Neurogenesis from parthenogenetic human embryonic stem cells |
| title_exact_search | Neurogenesis from parthenogenetic human embryonic stem cells |
| title_full | Neurogenesis from parthenogenetic human embryonic stem cells von Ruhel Ahmad |
| title_fullStr | Neurogenesis from parthenogenetic human embryonic stem cells von Ruhel Ahmad |
| title_full_unstemmed | Neurogenesis from parthenogenetic human embryonic stem cells von Ruhel Ahmad |
| title_short | Neurogenesis from parthenogenetic human embryonic stem cells |
| title_sort | neurogenesis from parthenogenetic human embryonic stem cells |
| topic | Zelldifferenzierung (DE-588)4067536-1 gnd Neurogenese (DE-588)4331352-8 gnd Embryonale Stammzelle (DE-588)4699648-5 gnd |
| topic_facet | Zelldifferenzierung Neurogenese Embryonale Stammzelle Hochschulschrift |
| url | https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-75935 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025739832&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT ahmadruhel neurogenesisfromparthenogenetichumanembryonicstemcells AT ahmadruhel neurogenesevonparthenogenetischenhumanenembryonalenstammzellen |