Mdc1 deletion accelerates telomere shortening, chromosomal instability, and premature aging in telomerase deficient mice:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 58 Bl. Ill., graph. Darst. |
Internformat
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| 245 | 1 | 0 | |a Mdc1 deletion accelerates telomere shortening, chromosomal instability, and premature aging in telomerase deficient mice |c by Satyavani Ravipati |
| 264 | 1 | |c 2012 | |
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| 650 | 4 | |a Telomere / Genetics | |
| 650 | 4 | |a Telomerase / Genetics | |
| 650 | 4 | |a Stem cell research | |
| 650 | 0 | 7 | |a Telomerase |0 (DE-588)4513124-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Telomer |g Molekulargenetik |0 (DE-588)4310952-4 |2 gnd |9 rswk-swf |
| 653 | |a Telomere | ||
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| 689 | 0 | 0 | |a Telomerase |0 (DE-588)4513124-7 |D s |
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Datensatz im Suchindex
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| adam_text | IMAGE 1
TABLE OF CONTENTS
ABSTRACT 7
INTRODUCTION 8
1.1 TELOMERES 9
1.2 TELOMERASE 10
1.3 THE SHELTERIN COMPLEX 11
1.4 SHELTERIN ACCESSORY FACTORS 12
1.5 THE TELOMERASE KNOCKOUT MOUSE MODEL 13
1.6 MDC1 (MEDIATOR OF DNA DAMAGE CHECKPOINT PROTEIN 1) 15
1.7 MDC1 KNOCKOUT MICE 18
1.8 TELOMERES AND DNA DAMAGE RESPONSE 19
MATERIALS AND METHODS 22
2.1 LABORATORY EQUIPMENT 23
2.2 PROGRAMMES 23
2.3 GENERATION OF MICE 24
2.4 GENOTYPING 24
2.5 MAINTENANCE AND HANDLING OF MICE 24
2.6 WHOLE MOUNTS 25
2.7 IMMUNOHISTOCHEMISTRY ON PARAFFIN SECTIONS 25
2.8 TUNEL ASSAY 25
2.9 ANAPHASE BRIDGE INDEX 26
2.10 QUANTITATIVE FLUORESCENT IN SITU HYBRIDIZATION (QFISH) 26
2.11 ARRAY-COMPARATIVE GENOMIC HYBRIDIZATION 26
2.12 FACS 27
2.13 COMPETITIVE TRANSPLANTATION ASSAY 28
5
HTTP://D-NB.INFO/1031907297
IMAGE 2
2.14 STATISTICAL ANALYSIS 28
RESULTS 29
3.1 MDC1 DEFICIENCY LIMITS THE LIFE SPAN OF TELOMERASE KNOCKOUT MICE 30
3.2 MDC1 DELETION IMPAIRS ORGAN MAINTENANCE IN AGING
TELOMERASE-DYSFUNCTIONAL MICE 33
3.3 PROLIFERATION AND APOPTOSIS IN MDC1 DEFICIENT AND TELOMERASE
DYSFUNCTIONAL MICE 34
3.4 MDC1 DELETION INCREASES THE INDUCTION OF 53BP1 IN TELOMERE
DYSFUNCTIONAL MICE 35
3.5 MDC1 DELETION INCREASES CHECKPOINT ACTIVATION IN TELOMERE
DYSFUNCTIONAL MICE 36
3.6 MDC1 DELETION LEADS TO INCREASED TELOMERE SHORTENING IN TELOMERE
DYSFUNCTIONAL MICE 37
3.7 MDC1 DELETION INCREASES ANAPHASE BRIDGE INDEX IN TELOMERE
DYSFUNCTIONAL MICE 38
3.8 MDC1 DELETION INCREASES CHROMOSOMAL INSTABILITY IN BASAL CRYPTS OF
TELOMERE DYSFUNCTIONAL MICE 39
3.9 MDC1 DELETION INCREASES CHECKPOINT ACTIVATION IN BASAL CRYPTS OF
TELOMERE DYSFUNCTIONAL MICE 40
3.10 MDC1 DELETION DECREASES THE NUMBER OF STEM CELL IN THE SMALL
INTESTINE OF TELOMERE DYSFUNCTIONAL MICE 41
3.11 MDC1 DELETION DOES NOT AFFECT THE HEMATOPOIETIC STEM CELL NUMBERS
IN TELOMERE DYSFUNCTIONAL MICE 42
3.12 MDC1 DELETION IMPAIRS THE REPOPULATION CAPACITY OF LT-HSCS IN
COMPETITIVE TRANSPLANTATION EXPERIMENTS 43
DISCUSSION 44
REFERENCES 50
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| author | Ravipati, Satyavani 1980- |
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| dewey-full | 572.86 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.86 |
| dewey-search | 572.86 |
| dewey-sort | 3572.86 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
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| spelling | Ravipati, Satyavani 1980- Verfasser (DE-588)1029626928 aut Mdc1 deletion accelerates telomere shortening, chromosomal instability, and premature aging in telomerase deficient mice by Satyavani Ravipati 2012 58 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Ulm, Univ., Diss., 2012 Telomere / Genetics Telomerase / Genetics Stem cell research Telomerase (DE-588)4513124-7 gnd rswk-swf Telomer Molekulargenetik (DE-588)4310952-4 gnd rswk-swf Telomere (DE-588)4113937-9 Hochschulschrift gnd-content Telomerase (DE-588)4513124-7 s Telomer Molekulargenetik (DE-588)4310952-4 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025726639&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Ravipati, Satyavani 1980- Mdc1 deletion accelerates telomere shortening, chromosomal instability, and premature aging in telomerase deficient mice Telomere / Genetics Telomerase / Genetics Stem cell research Telomerase (DE-588)4513124-7 gnd Telomer Molekulargenetik (DE-588)4310952-4 gnd |
| subject_GND | (DE-588)4513124-7 (DE-588)4310952-4 (DE-588)4113937-9 |
| title | Mdc1 deletion accelerates telomere shortening, chromosomal instability, and premature aging in telomerase deficient mice |
| title_auth | Mdc1 deletion accelerates telomere shortening, chromosomal instability, and premature aging in telomerase deficient mice |
| title_exact_search | Mdc1 deletion accelerates telomere shortening, chromosomal instability, and premature aging in telomerase deficient mice |
| title_full | Mdc1 deletion accelerates telomere shortening, chromosomal instability, and premature aging in telomerase deficient mice by Satyavani Ravipati |
| title_fullStr | Mdc1 deletion accelerates telomere shortening, chromosomal instability, and premature aging in telomerase deficient mice by Satyavani Ravipati |
| title_full_unstemmed | Mdc1 deletion accelerates telomere shortening, chromosomal instability, and premature aging in telomerase deficient mice by Satyavani Ravipati |
| title_short | Mdc1 deletion accelerates telomere shortening, chromosomal instability, and premature aging in telomerase deficient mice |
| title_sort | mdc1 deletion accelerates telomere shortening chromosomal instability and premature aging in telomerase deficient mice |
| topic | Telomere / Genetics Telomerase / Genetics Stem cell research Telomerase (DE-588)4513124-7 gnd Telomer Molekulargenetik (DE-588)4310952-4 gnd |
| topic_facet | Telomere / Genetics Telomerase / Genetics Stem cell research Telomerase Telomer Molekulargenetik Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025726639&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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