Fungi and NO defense?: Mycoflora in brood cells of the European Beewolf and its radical defense
Gespeichert in:
1. Verfasser: | |
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Format: | Abschlussarbeit Buch |
Sprache: | English |
Veröffentlicht: |
2011
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Schlagworte: | |
Online-Zugang: | Volltext https://nbn-resolving.org/urn:nbn:de:bvb:355-epub-234208 Inhaltsverzeichnis |
Beschreibung: | 101 S. Ill., graph. Darst. |
Internformat
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Datensatz im Suchindex
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adam_text | IMAGE 1
CONTENTS
CHAPTER 1: GENERAL INTRODUCTION 1
1.1 THE MICROBIAL THREAT & INSECT DEFENSE 1
1.2 THE BIOLOGY OF PHILANTHUS TRIANGULUM 2
1.3 OBJECTIVES OF THE THESIS 4
1.4 REFERENCES 4
CHAPTER 2: MYCOFLORA OF BEEWOLF BROOD CELLS 9
2.1 INTRODUCTION 9
2.2 MATERIALS & METHODS 11
ISOLATION OF FUNGI 11
DNA PREPARATION, PCR, SEQUENCING 11
MICROSCOPY 12
2.3 RESULTS 12
2.4 DISCUSSION 15
CHAPTER 3: NO DEFENSE AGAINST FUNGI 21
3.1 INTRODUCTION 21
3.2 MATERIALS & METHODS 23
BEEWOLF SPECIMENS 23
3.2.1 IDENTIFICATION OF THE GAS AND ITS SOURCE 23
IDENTIFICATION OF THE GAS 23
QUANTIFICATION, TIME COURSE AND TEMPERATURE DEPENDENCE OF GAS PRODUCTION
24
DETECTION AND LOCALIZATION OF NITRIC OXIDE INSIDE THE EGG 25
RNA EXTRACTION, REVERSE TRANSCRIPTION AND QUANTITATIVE PCR 26
NOS INHIBITION ASSAY 27
METABOLIC RATE OF EGGS 27
3.2.1 EFFECT ON MICROORGANISMS 28
3.3 RESULTS 29
3.3.1 IDENTIFICATION OF THE GAS AND ITS SOURCE 29
NITRITE TEST 29
QUANTIFICATION, TIME COURSE AND TEMPERATURE DEPENDENCE OF GAS PRODUCTION
30
DETECTION AND LOCALIZATION OF NITRIC OXIDE INSIDE THE EGG 32
NADPH DIAPHORASE STAINING 32
HTTP://D-NB.INFO/1031945741
IMAGE 2
NOS EXPRESSION 32
NOS INHIBITION ASSAY 36
RATE OF METABOLISM 36
3.3.2 EFFECT OF GAS ON MICROORGANISMS 38
3.4 DISCUSSION 40
IDENTIFICATION AND SOURCE OF THE GAS 40
TEMPORAL COURSE & LOCALIZATION 4 1
EFFECT ON MICROORGANISMS 43
SELF-DEFENSE 45
3.5 REFERENCES 46
CHAPTER 4: CAN NO DEFENSE BE COSTLY? 51
4.1 INTRODUCTION 51
4.2 MATERIALS & METHODS 53
BEEWOLF SPECIMEN 53
QUANTIFICATION OF NITRIC OXIDE RELEASE 53
MEASUREMENTS FOR THE PATH ANALYSIS 53
STATISTICS 54
4.3 RESULTS 54
4.4 DISCUSSION 58
FORMER INVESTMENT REDUCES NITRIC OXIDE DEFENSE 58
DEFENSE VERSUS NUMBER OF OFFSPRING - IN BETWEEN TWO STRATEGIES 59
ADAPTIVE DEFENSE 60
4.5 REFERENCES 61
1
CHAPTER 5: DEFENSE IS THE SECOND FACET OF PARENTAL INVESTMENT 63
5.1 INTRODUCTION 63
5.2 METHODS 64
5.3 RESULTS 67
5.4 DISCUSSION 71
CHAPTER 6: GENERAL DISCUSSION 79
6.1 NITRIC OXIDE AND BEEWOLF EGGS 79
6.2 THE COST OF DEFENSE 8 1
6.3 POTENTIAL COSTS FOR THE PROGENY 83
IMAGE 3
6.4 COSTS FOR THE MOTHER 84
6.5 THE PROBLEM OF RESOURCE ALLOCATION AND THE ADVANTAGES OF A
MULTIFACETED DEFENSE 86
6.6 SYNOPSIS OF THE BEEWOLF DEFENSE 88
6.7 REFERENCES 89
CHAPTER 7 SUMMARY 97
CHAPTER 8 ZUSAMMENFASSUNG 99
DANKSAGUNG 101
|
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author | Engl, Tobias 1980- |
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discipline | Biologie |
format | Thesis Book |
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spelling | Engl, Tobias 1980- Verfasser (DE-588)1030483272 aut Fungi and NO defense? Mycoflora in brood cells of the European Beewolf and its radical defense vorgelegt von Tobias Engl 2011 101 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Regensburg, Univ., Diss., 2011 Brutfürsorge (DE-588)4146772-3 gnd rswk-swf Stickstoffmonoxid (DE-588)4183282-6 gnd rswk-swf Schimmelpilze (DE-588)4052495-4 gnd rswk-swf Bienenwolf (DE-588)4145352-9 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Bienenwolf (DE-588)4145352-9 s Brutfürsorge (DE-588)4146772-3 s Schimmelpilze (DE-588)4052495-4 s Stickstoffmonoxid (DE-588)4183282-6 s b DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:355-epub-234208 http://epub.uni-regensburg.de/23420/ Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:355-epub-234208 Resolving-System DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025701416&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
spellingShingle | Engl, Tobias 1980- Fungi and NO defense? Mycoflora in brood cells of the European Beewolf and its radical defense Brutfürsorge (DE-588)4146772-3 gnd Stickstoffmonoxid (DE-588)4183282-6 gnd Schimmelpilze (DE-588)4052495-4 gnd Bienenwolf (DE-588)4145352-9 gnd |
subject_GND | (DE-588)4146772-3 (DE-588)4183282-6 (DE-588)4052495-4 (DE-588)4145352-9 (DE-588)4113937-9 |
title | Fungi and NO defense? Mycoflora in brood cells of the European Beewolf and its radical defense |
title_auth | Fungi and NO defense? Mycoflora in brood cells of the European Beewolf and its radical defense |
title_exact_search | Fungi and NO defense? Mycoflora in brood cells of the European Beewolf and its radical defense |
title_full | Fungi and NO defense? Mycoflora in brood cells of the European Beewolf and its radical defense vorgelegt von Tobias Engl |
title_fullStr | Fungi and NO defense? Mycoflora in brood cells of the European Beewolf and its radical defense vorgelegt von Tobias Engl |
title_full_unstemmed | Fungi and NO defense? Mycoflora in brood cells of the European Beewolf and its radical defense vorgelegt von Tobias Engl |
title_short | Fungi and NO defense? |
title_sort | fungi and no defense mycoflora in brood cells of the european beewolf and its radical defense |
title_sub | Mycoflora in brood cells of the European Beewolf and its radical defense |
topic | Brutfürsorge (DE-588)4146772-3 gnd Stickstoffmonoxid (DE-588)4183282-6 gnd Schimmelpilze (DE-588)4052495-4 gnd Bienenwolf (DE-588)4145352-9 gnd |
topic_facet | Brutfürsorge Stickstoffmonoxid Schimmelpilze Bienenwolf Hochschulschrift |
url | http://epub.uni-regensburg.de/23420/ https://nbn-resolving.org/urn:nbn:de:bvb:355-epub-234208 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025701416&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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