siRNA design: methods and protocols
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
New York [u.a.]
Humana Press, Springer
2013
|
| Series: | Methods in molecular biology
942 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Includes bibliographical references and index |
| Physical Description: | XIII, 392 S. Ill., graph. Darst. |
| ISBN: | 9781627031189 |
Staff View
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| 245 | 1 | 0 | |a siRNA design |b methods and protocols |c ed. by Debra J. Taxman |
| 264 | 1 | |a New York [u.a.] |b Humana Press, Springer |c 2013 | |
| 300 | |a XIII, 392 S. |b Ill., graph. Darst. | ||
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| 650 | 4 | |a Small interfering RNA | |
| 650 | 4 | |a RNA, Small Interfering |x genetics |v Laboratory Manuals | |
| 650 | 4 | |a Gene Silencing |v Laboratory Manuals | |
| 650 | 4 | |a Gene Targeting |x methods |v Laboratory Manuals | |
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Record in the Search Index
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|---|---|
| adam_text | Titel: siRNA design
Autor: Taxman, Debra J
Jahr: 2013
Contents
Preface.............................................................. v
Contributors.......................................................... xi
1 What Parameters to Consider and Which Software Tools to Use
for Target Selection and Molecular Design of Small Interfering RNAs....... 1
Olga Matveeva
2 Methods for Selecting Effective siRNA Target Sequences Using
a Variety of Statistical and Analytical Techniques....................... 17
Shigeru Takasaki
3 Designing Functional siRNA with Reduced Off-Target Effects............ 57
Tuki Naito and Kumiko Ui-Tei
4 Design and Screening of siRNAs Against Highly
Structured RNA Targets......................................... 69
Neda Nasheri, John Paul Pezacki, and Selena M. Sagan
5 Engineering Small Interfering RNAs by Strategic
Chemical Modification.......................................... 87
Jesper B. Bramsen andj$rgen Kjems
6 The Design, Selection, and Evaluation of Highly Specific
and Functional siRNA Incorporating Unlocked Nucleobase Analogs........ Ill
Narendra Vaish and Pinky Agarwal
7 The Design, Preparation, and Evaluation of Asymmetric Small
Interfering RNA for Specific Gene Silencing in Mammalian Cells.......... 135
Chanil Chang, Sun Woo Hong, Pooja Dua,
Soyoun Kim, and Dong-ki Lee
8 Design of Nuclease-Resistant Fork-Like Small Interfering
RNA (fsiRNA)................................................ 153
Elena L. Chemolovskaya and Marina A. Zenkova
9 Designing Dual-Targeting siRNA Duplexes Having Two Active
Strands that Combine siRNA and MicroRNA-Like Targeting............. 169
Pal S trom
10 Strategies for Designing and Validating Immunostimulatory siRNAs........ 179
Michael P. Gantier
11 Designing Efficient and Specific Endoribonuclease-Prepared siRNAs........ 193
Vineeth Surendranath, Mirko Theis, Bianca H Habermann,
and Frank Buchholz
12 Short Hairpin RNA-Mediated Gene Silencing......................... 205
Luke S. Lambeth and Craig A. Smith
IX
x Contents
13 Design of Lentivirally Expressed siRNAs............................. 233
Ting Poi Liu and Ben Berkhout
14 Bifunctional Short Hairpin RNA (bi-shRNA): Design and Pathway
to Clinical Application........................................... 259
Donald D. Rao, Neil Senzer, Zhaohui Wang, Padmasini Kumar,
Chris M. Jay, and John Nemunaitis
15 Design and Chemical Modification of Synthetic Short shRNAs
as Potent RNAi Triggers......................................... 279
Anne Dallas and Brian H. Johnston
16 Production and Application of Long dsRNAin Mammalian Cells.......... 291
Katerina Chalupnikova, Jana Nejepinska, and Petr Svoboda
17 Design of RNAi Reagents for Invertebrate Model Organisms
and Human Disease Vectors...................................... 315
Thomas Horn and Michael Boutros
18 Construction of shRNA Expression Plasmids for Silkworm Cell
Lines Using Single-Stranded DNA and JSxtDNA Polymerase.............. 347
Hiromitsu Tanaka
19 Designing Effective amiRNA and Multimeric
amiRNA Against Plant Viruses.................................... 357
Muhammad Fahim and Philip J. Larkin
20 Downregulation of Plant Genes with miRNA-Induced Gene Silencing...... 379
Felipe Fenselau de Felippes
Index........................................................... 389
|
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| dewey-full | 572.88 |
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| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.88 |
| dewey-search | 572.88 |
| dewey-sort | 3572.88 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Book |
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| id | DE-604.BV040701341 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T00:32:01Z |
| institution | BVB |
| isbn | 9781627031189 |
| language | English |
| lccn | 2012947406 |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-025681866 |
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| physical | XIII, 392 S. Ill., graph. Darst. |
| publishDate | 2013 |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | siRNA design methods and protocols ed. by Debra J. Taxman New York [u.a.] Humana Press, Springer 2013 XIII, 392 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 942 Springer protocols Includes bibliographical references and index Small interfering RNA RNA, Small Interfering genetics Laboratory Manuals Gene Silencing Laboratory Manuals Gene Targeting methods Laboratory Manuals Silencer (DE-588)4378881-6 gnd rswk-swf Molekulardesign (DE-588)4265444-0 gnd rswk-swf RNS-Interferenz (DE-588)4802706-6 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Laboratory Manuals RNS-Interferenz (DE-588)4802706-6 s Silencer (DE-588)4378881-6 s Molekulardesign (DE-588)4265444-0 s Methode (DE-588)4038971-6 s b DE-604 Taxman, Debra J. Sonstige oth Erscheint auch als Online-Ausgabe 978-1-62703-119-6 Methods in molecular biology 942 (DE-604)BV035362695 942 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025681866&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | siRNA design methods and protocols Methods in molecular biology Small interfering RNA RNA, Small Interfering genetics Laboratory Manuals Gene Silencing Laboratory Manuals Gene Targeting methods Laboratory Manuals Silencer (DE-588)4378881-6 gnd Molekulardesign (DE-588)4265444-0 gnd RNS-Interferenz (DE-588)4802706-6 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4378881-6 (DE-588)4265444-0 (DE-588)4802706-6 (DE-588)4038971-6 |
| title | siRNA design methods and protocols |
| title_auth | siRNA design methods and protocols |
| title_exact_search | siRNA design methods and protocols |
| title_full | siRNA design methods and protocols ed. by Debra J. Taxman |
| title_fullStr | siRNA design methods and protocols ed. by Debra J. Taxman |
| title_full_unstemmed | siRNA design methods and protocols ed. by Debra J. Taxman |
| title_short | siRNA design |
| title_sort | sirna design methods and protocols |
| title_sub | methods and protocols |
| topic | Small interfering RNA RNA, Small Interfering genetics Laboratory Manuals Gene Silencing Laboratory Manuals Gene Targeting methods Laboratory Manuals Silencer (DE-588)4378881-6 gnd Molekulardesign (DE-588)4265444-0 gnd RNS-Interferenz (DE-588)4802706-6 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | Small interfering RNA RNA, Small Interfering genetics Laboratory Manuals Gene Silencing Laboratory Manuals Gene Targeting methods Laboratory Manuals Silencer Molekulardesign RNS-Interferenz Methode |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025681866&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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