Retinal degeneration: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Humana Press, Springer
2013
|
| Schriftenreihe: | Methods in molecular biology
935 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Klappentext |
| Beschreibung: | XIV, 384 S. Ill., graph. Darst. |
| ISBN: | 1627030794 9781627030793 |
Internformat
MARC
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| 020 | |a 9781627030793 |c hbk. |9 978-1-62703-079-3 | ||
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| 245 | 1 | 0 | |a Retinal degeneration |b methods and protocols |c ed. by Bernhard H. F. Weber ... |
| 264 | 1 | |a New York [u.a.] |b Humana Press, Springer |c 2013 | |
| 300 | |a XIV, 384 S. |b Ill., graph. Darst. | ||
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| 490 | 1 | |a Methods in molecular biology |v 935 | |
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Datensatz im Suchindex
| _version_ | 1804149997698023424 |
|---|---|
| adam_text | Contents
Preface
..........................................................
v
Contributors.
..................................................... xi
Part I Human Genetics
1
Identification and Analysis of Inherited Retinal Disease Genes
............. 3
Korneli«
Neveling,
Anneke I. den Hollander,
Frans P.M. Cremen,
andRohW.J.CoUin
Part II Mouse Models
2
Mouse Models for Studies of Retinal Degeneration and Diseases
........... 27
Bo Chang
3
Retinal
Fundus
Imaging in Mouse Models of Retinal Diseases
............. 41
Anne F. Alex, Peter Heiduscbka, and Nicole
Eter
4
Functional Phenotyping of Mouse Models with ERG
................... 69
Naoyuki Tanimoto, Vitbiyanjali Sothilingam, and
Mathias W. Seeliger
5
Phenotyping of Mouse Models with
ОСТ
............................ 79
M.
Dominik
Fischer, Ahmad Zhour, and
Christoph J. Kernstock
6
Light Damage as a Model of Retinal Degeneration
..................... 87
Christian Grimm and Charlotte
E. Remi
7
N-Mcthyl-n-Aspartate (NMDA)-Mediated Excitotoxic Damage:
A Mouse Model of Acute Retinal Ganglion Cell Damage
................ 99
Roswitha Seite
and
Ema R.
Татт
Part III Other Animal Models
8
Generation of Transgenic X.
larris
Modcb of Retinal Degeneration
........ 113
Beatrice
M
Tam,
Christine C.-L·
lai,
Zmsheng
Zong,
and Orson
L·
Morim
9
Analysis of Photoreceptor Degeneration in die Zcbrafish
Danio rerio
....... 127
HofyerDiU,
Bastian
Under,
Anja Hirmer,
and
Uta
Fischer
10
Analysis of Optokinetic Response in Zcbrafish
by Computer-Based Eye Tracking
.................................. 139
Sabina
P. Hnber-Reggi,
Kaspar
P. Mueller, and
Stephan
CF. Neubauss
11
Analysis of the
Drosophila
Compound Eye with light
and Electron Microscopy
........................................ 161
MonaUsa Mishra and Elisabeth
Knust
viii Contents
Part IV In Situ Analyses
12
Cell-Specific Markers for the Identification of Retinal Cells
by Immunofluorescence Microscopy
................................ 185
Christiana L· Cheng, Hidayat Djajadi, and Robert S. Molday
13
A Method of Horizontally Sliced Preparation of the Retina
............... 201
Ryosuke Enoki and
Amane
Koizumi
14
Detection of
DNA
Fragmentation in Retinal Apoptosis by
TUNEL
........ 207
Francesca Doonan and Thomas G. Cotter
15
High-Throughput
RNA
In Situ Hybridization in Mouse Retina
........... 215
Setít
Blackshaw
16
Assessment of Mitochondrial Damage in Retinal Cells and Tissues
Using Quantitative Polymerase Chain Reaction for Mitochondrial
DNA
Damage and Extracellular Flux Assay for Mitochondrial
Respiration Activity
............................................. 227
Stuart G.Jarrett,
Bärbel Rohrer,
Nathan
R.
Perron,
Craig
Beesőn,
and Michael E. Boulton
17
Analysis of Photoreceptor Rod Outer Segment Phagocytosis
by RPE CeUs In Situ
............................................ 245
Saumil Sethna and Silvia C.
Finnemann
Part V Tissue Culture and Cell Models
18
Ca2+ Microfluorimetry in Retinal
Müller Glial
Cells
..................... 257
Antje Wurm,
Thomas Pannicke, and Andreas
Reichenbach
19
Functional Analysis of Retinal Microglia and Their Effects
on Progenitors
................................................ 271
Debra A. Carter, Balini Balasubramaniam, and Andrew D. Dick
20
Analysis of Photoreceptor Outer Segment Phagocytosis
by RPE Cells in Culture
......................................... 285
Tingyu Mao ana Silvia
С
Finnemann
21
Ca2+-Imaging Techniques to Analyze
Ca2ł
Signaling in CeUs
and to Monitor
Neuronal
Activity in die Retina
....................... 297
Olaf Strauß
Part VI Gene Regulation
22
Double Chromatin Immunoprecipitation: Analysis of Target
Co-occupancy of Retinal Transcription Factors
........................ 311
Guang-Hua Peng and Shiming Chen
23
Quantifying the Activity of cw-Regulatory Elements in the Mouse Retina
by
Explant Electroporation
....................................... 329
Cynthia L· Montana, Connie A. Myers, and Joseph C. Corbo
Contents ix
Part
VII
Therapy
24
Optimized Technique for Subretinal Injections in Mice
.................. 343
Regine
Mtthlfriedel, Stylianos Michalakis, Marina Garcia
Garrido,
Martin
Biel,
and
Mathias W. Seeliger
25
Adeno-Associated Viral Vectors for Gene Therapy of Inherited
Retinal Degenerations
........................................... 351
John G. Flannery and Meike
Visel
26
Barrier Modulation in Drug Delivery to the Retina
..................... 371
Matthew Campbell, Marian M. Humphries, and Peter Humphries
Index
........................................................... 381
Methods in Molecular Biology
935
John M. Walker, Series Editor
Bernhard
H. F.
Weber
■
Thomas
Langmann
Editors
fietinaf Degeneration
Methods and Protocols
Over the past decades, knowledge about the cellular and molecular basis underlying the visual
process has remarkably increased. In Retinal Degeneration: Methods and Protocols, expert re¬
searchers in the field provide a guide of relevant and state-of-the-art methods for studying
retinal homeostasis and disease. These include methods and techniques
for
addressing cell cul¬
ture systems and animal models of disease, their generation, their phenotypic and molecular
characterization as well as their use in therapeutic approaches to the retina. Written in the highly
successful Methods in Molecular Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible
laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Retinal Degeneration: Methods and Protocols aids scientists in con¬
tinuing to study the cutting-edge techniques of retinal cell biology in health and disease.
|
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| isbn | 1627030794 9781627030793 |
| language | English |
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| spelling | Retinal degeneration methods and protocols ed. by Bernhard H. F. Weber ... New York [u.a.] Humana Press, Springer 2013 XIV, 384 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 935 Springer protocols Netzhautdegeneration (DE-588)4171515-9 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Netzhautdegeneration (DE-588)4171515-9 s DE-604 Weber, Bernhard H. F. Sonstige (DE-588)1061058050 oth Erscheint auch als Online-Ausgabe 978-1-62703-080-9 Methods in molecular biology 935 (DE-604)BV035362695 935 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025677641&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025677641&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Klappentext |
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| subject_GND | (DE-588)4171515-9 (DE-588)4143413-4 |
| title | Retinal degeneration methods and protocols |
| title_auth | Retinal degeneration methods and protocols |
| title_exact_search | Retinal degeneration methods and protocols |
| title_full | Retinal degeneration methods and protocols ed. by Bernhard H. F. Weber ... |
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| title_short | Retinal degeneration |
| title_sort | retinal degeneration methods and protocols |
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