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Ion channels: methods and protocols

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Bibliographische Detailangaben
Weitere Verfasser:	Gamper, Nikita (HerausgeberIn)
Format:	Buch
Sprache:	English
Veröffentlicht:	New York [u.a.] Humana Press 2013
Ausgabe:	2. ed.
Schriftenreihe:	Methods in molecular biology 998
Schlagworte:	Ion Channels Molekularbiologische Methode Ionenkanal
Online-Zugang:	Inhaltsverzeichnis
Beschreibung:	XIV, 440 S. Ill., graph. Darst.
ISBN:	9781627033503 9781627033510

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Datensatz im Suchindex

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adam_text	Titel: Ion channels Autor: Gamper, Nikita Jahr: 2013 Contents Preface.............................................................. v Contributors.......................................................... xi Part I Cloning and Heterologous Expression of Ion Channel Genes 1 Approaches to Cloning of Pain-Related Ion Channel Genes.................. 3 Armen N. Akopian 2 Mammalian Expression Systems and Transaction Techniques................. 21 Daunia Laurenti and Lezanne Ooi 3 Use of Escherichia coli for the Production and Purification of Membrane Proteins.............................................. 33 Vincent G.L. Postis, Andrea E. Rawlings, Amelia Lesiuk, and Stephen A. Baldwin 4 Transient Overexpression of Genes in Neurons Using Nucleofection............ 55 Hannah M. Kirton, Louisa Pettinger, and Nikita Gamper 5 Viral Gene Delivery: Optimized Protocol for Production of High Titer Lentiviral Vectors....................................... 65 James Hewinson, Julian F.R. Paton, and Sergey Kasparov Part II Electrophysiological Methods to Study Ion Channel Functions 6 Two-Electrode Voltage Clamp........................................ 79 Bingcai Guan, Xingjuan Chen, and Hailin Zhang 7 Conventional Micropipette-Based Patch Clamp Techniques.................. 91 Jonathan D. Lippiat and David C. Wrighton 8 Recording of Ion Channel Activity in Planar Lipid Bilayer Experiments.......... 109 Eleonora Zakharian 9 Recording Macroscopic Currents in Large Patches from Xenopus Oocytes.............................................. 119 Tibor Rohacs 10 Combined Single-Channel and Macroscopic Recording Techniques to Analyze Gating Mechanisms of the Large Conductance Ca2+ and Voltage Activated (BK) Potassium Channel........................ 133 Nguyen V. Nguyen, Aleksandra Gruslova, Wojciech A. Kosiba, and Bin Wang 11 Perforated Whole-Cell Patch-Clamp Recording........................... 149 John E. Linley 12 Piezo-Electrically Driven Mechanical Stimulation of Sensory Neurons........... 159 Jizhe Hao, Jerome Ruel, Bertrand Coste, Tann Roudaut, Marcel Crest, and Patrick Delmas vii vili Contents 13 Automated Planar Patch-Clamp....................................... 171 Carol J. Millikan and Clemens Möller 14 Recording Single-Channel Currents Using Smart Patch-Clamp Technique...................................... 189 Anamika Bhargava and Julia Gorelik Part III Imaging and Fluorescence Methods to Study Ion Channels 15 Using Total Internal Reflection Fluorescence Microscopy to Observe Ion Channel Trafficking and Assembly......................... 201 Sarah Schwarzer, Gregory I. Mashanov, Justin E. Molloy, and Andrew Tinker 16 Förster Resonance Energy Transfer-Based Imaging at the Cell Surface of Live Cells....................................... 209 Sonya M. Bierbower and Mark S. Shapiro 17 The Use of Dansyl-Calmodulin to Study Interactions with Channels and Other Proteins..................................... 217 Alessandro Alaimo, Covadonga Malo, Pilar Areso, Kerman Aloria, Oscar Millet, and Alvaro Villarroel 18 Imaging and Quantification of Recycled KATp Channels...................... 233 Christofher J. Cocker oft Part IV Biochemical and Structure-Functional Approaches in Ion Channel Studies 19 Generation of Antibodies That Are Externally Acting Isoform-Specific Inhibitors of Ion Channels.............................. 245 Jacqueline Naylor and David J. Beech 20 Site-Directed Mutagenesis to Study the Structure-Function Relationships of Ion Channels......................................... 257 Wei Tang and Lin-Hua Jiang 21 Cysteine-Based Cross-Linking Approach to Study Inter-domain Interactions in Ion Channels............................... 267 Lin-Hua Jiang 22 Analysis of Ca2+ Binding Sites in the MthK RCK Domain by X Ray Crystallography............................................ 277 Frank J. Smith and Brad S. Rothberg 23 Isotope Labeling Strategies for Analysis of an Ion Channel Cytoplasmic Domain by NMR Spectroscopy....................... 289 Karin Abarca-Heidemann, Elke Duchardt-Ferner, Jens Woehnert, and Brad S. Rothberg Part V Studying Ion Channels in Native Tissues 24 Recording Dendritic Ion Channel Properties and Function from Cortical Neurons.............................................. 303 Mala M. Shah Contents ix 25 M-Current Recording from Acute DRG Slices............................ 311 Kirstin E. Rose, Sylvain Gigout, and Nikita Gamper 26 Studying Ion Channels in Human Erythrocytes by Direct and Indirect Means......................................... 321 Stephan M. Huber 27 Recording Ion Channels in Isolated, Split-Opened Tubules................... 341 Elena Mironova, Vladislav Bug ay, Oleh Pochynyuk, Alexander Staruschenko, and James D. Stockand 28 Single-Channel Analysis of TRPC Channels in the Podocytes of Freshly Isolated Glomeruli............................ 355 Daria V. Ilatovskaya and Alexander Staruschenko 29 Ca2+ Imaging as a Tool to Assess TRP Channel Function in Murine Distal Nephrons........................................... 371 Mykola Mamenko, Ole# Zaika, Roger G. O Neil, and Oleh Pochynyuk 30 Patch-Clamping Drosophila Sensory Neurons............................. 385 Volodymyr Kucher, Benjamin A. Eaton, James D. Stockand, and Nina Boiko Part VI Ion Channels as Research Tools 31 Production and Validation of Recombinant Adeno-Associated Virus for Channelrhodopsin Expression in Neurons............................. 401 John Y. Lin 32 Optical Control of Ligand-Gated Ion Channels........................... 417 Stephanie Szobota, Catherine McKenzie, and Harald Janovjak Index................................................................... 437
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dewey-full	571.6
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dewey-sort	3571.6
dewey-tens	570 - Biology
discipline	Biologie
edition	2. ed.
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spelling	Ion channels methods and protocols ed. by Nikita Gamper 2. ed. New York [u.a.] Humana Press 2013 XIV, 440 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 998 Springer protocols Ion Channels Molekularbiologische Methode (DE-588)7650063-9 gnd rswk-swf Ionenkanal (DE-588)4138699-1 gnd rswk-swf Ionenkanal (DE-588)4138699-1 s Molekularbiologische Methode (DE-588)7650063-9 s b DE-604 Gamper, Nikita edt Methods in molecular biology 998 (DE-604)BV035362695 998 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025670290&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Ion channels methods and protocols Methods in molecular biology Ion Channels Molekularbiologische Methode (DE-588)7650063-9 gnd Ionenkanal (DE-588)4138699-1 gnd
subject_GND	(DE-588)7650063-9 (DE-588)4138699-1
title	Ion channels methods and protocols
title_auth	Ion channels methods and protocols
title_exact_search	Ion channels methods and protocols
title_full	Ion channels methods and protocols ed. by Nikita Gamper
title_fullStr	Ion channels methods and protocols ed. by Nikita Gamper
title_full_unstemmed	Ion channels methods and protocols ed. by Nikita Gamper
title_short	Ion channels
title_sort	ion channels methods and protocols
title_sub	methods and protocols
topic	Ion Channels Molekularbiologische Methode (DE-588)7650063-9 gnd Ionenkanal (DE-588)4138699-1 gnd
topic_facet	Ion Channels Molekularbiologische Methode Ionenkanal
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