Cellular response to exposure with different radiation qualities with a focus on nuclear factor kappa-B:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 353 S. Ill., graph. Darst. |
Internformat
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| 100 | 1 | |a Hellweg, Christine E. |d 1971- |e Verfasser |0 (DE-588)123502578 |4 aut | |
| 245 | 1 | 0 | |a Cellular response to exposure with different radiation qualities with a focus on nuclear factor kappa-B |c eingereicht von Christine E. Hellweg |
| 264 | 1 | |c 2012 | |
| 300 | |a 353 S. |b Ill., graph. Darst. | ||
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| 650 | 7 | |a radiation |2 cabt | |
| 650 | 7 | |a cells |2 cabt | |
| 650 | 7 | |a cytotoxicity |2 cabt | |
| 650 | 7 | |a radiotherapy |2 cabt | |
| 650 | 7 | |a ultraviolet radiation |2 cabt | |
| 650 | 7 | |a ions |2 cabt | |
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Datensatz im Suchindex
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| adam_text | IMAGE 1
CONTENT
1 INTRODUCTION 7
1.1 RADIATION EXPOSURE DURING SPACE MISSIONS 8
1.1.1 RADIATION QUALITIES AND LINEAR ENERGY TRANSFER 8
1.1.2 RADIATION EXPOSURE ON THE INTERNATIONAL SPACE STATION 11
1.1.3 RADIATION EXPOSURE ON MISSIONS TO MOON OR MARS 13
1.1.4 EFFECTS OF SHIELDING 14
1.2 RADIATION EFFECTS ON HUMANS AND ANIMALS 15
1.2.1 ACUTE RADIATION SYNDROME 15
1.2.2 ACUTE EFFECTS OF PARTIAL BODY IRRADIATION 18
1.2.3 LATE AND CHRONIC RADIATION EFFECTS 18
1.3 CELLULAR RADIATION RESPONSE 21
1.3.1 RADIATION-INDUCED DNA DAMAGE 23
1.3.2 REPAIR OF RADIATION-INDUCED DNA DAMAGE 24
1.3.3 DNA DAMAGE PROCESSING 26
1.3.4 RADIATION-INDUCED CELL CYCLE ARRESTS 30
1.3.5 RADIATION-INDUCED CELL DEATH AND DEATH BYPASS MECHANISMS 33
1.3.6 ACTIVATION OF TRANSCRIPTION FACTORS AND GENE EXPRESSION AFTER
RADIATION EXPOSURE. 35
1.3.7 CELLULAR RESPONSE TO HIGH-LET RADIATION 49
1.4 CANCER RADIOTHERAPY 53
1.4.1 CONVENTIONAL RADIOTHERAPY IN HUMAN AND VETERINARY MEDICINE 54
1.4.2 ION BEAM RADIOTHERAPY 54
1.5 RISK ASSESSMENT FOR ASTRONAUTS 58
1.5.1 CELLULAR EFFECTS OF MICROGRAVITY 58
1.5.2 COMBINED EFFECTS OF RADIATION AND MICROGRAVITY 59
1.5.3 SIMULATED MICROGRAVITY 61
1.6 AIMS AND TASKS 61
2 RESULTS AND DISCUSSION 67
2.1 THE SPACE EXPERIMENT CELLRAD 6 7
2.2 RECOMBINANT CELL LINE FOR MONITORING OF CELL PROLIFERATION 74
2.2.1 IMPACT ON TOXICITY TESTING 76
2.3 RECOMBINANT CELL LINE FOR INVESTIGATION OF NF-KB-DEPENDENT GENE
EXPRESSION INDUCTION 77
2.3.1 HUMAN EMBRYONIC KIDNEY CELLS 77
2.3.2 STABLE TRANSFECTION OF HUMAN EMBRYONIC KIDNEY CELLS WITH N F - K B
REPORTER PLASMIDS 79
2.3.3 HALF-LIFE OF THE REPORTER PROTEINS 79
2.3.4 POSSIBLE TOXIC EFFECTS OF FLUORESCENT REPORTER PROTEINS 80
2.3.5 N F - K B ACTIVATION BY DIFFERENT AGENTS 81
2.3.6 IMPACT ON DRUG DISCOVERY 83
2 . 3 . 7 REPORTING N F - K B ACTIVATION IN THREE-DIMENSIONAL CULTURE
AND IN VIVO 8 3 2.4 CELLULAR RESPONSE TO UV RADIATION 84
2.5 CELLULAR RESPONSE TO SPARSELY IONIZING RADIATION 85
2.6 EFFECTS OF SPACE- AND THERAPY-RELEVANT RADIATION QUALITIES 87
5
HTTP://D-NB.INFO/1033853895
IMAGE 2
2.6.1 CELL SURVIVAL AND DEATH AFTER ENERGETIC HEAVY ION EXPOSURE 87
2.6.2 ACTIVATION OF NF-KB-DEPENDENT REPORTER GENE EXPRESSION BY
ENERGETIC HEAVY IONS 89
2.6.3 GENE EXPRESSION MODULATION AFTER HIGH LET RADIATION EXPOSURE 92
2.6.4 CELL CYCLE PROGRESSION AFTER RADIATION EXPOSURE 94
2.6.5 RELATIVE BIOLOGICAL EFFECTIVENESS OF DIFFERENT ENERGETIC HEAVY
IONS 96
2 . 7 ENHANCEMENT AND MITIGATION OF N F - K B ACTIVATION 9 8
2.7.1 POTENTIAL OF N F - K B ACTIVATION FOR ACUTE RADIATION SYNDROME
PREVENTION AND
TREATMENT 98
2.7.2 POTENTIAL OF N F - K B INHIBITION FOR CANCER PREVENTION 99
2 . 7 . 3 INHIBITION OF N F - K B IN RADIATION THERAPY OF CANCER 1 0 0
2.8 CONCLUSION AND OUTLOOK 107
3 SUMMARY 109
4 ZUSAMMENFASSUNG 112
5 REFERENCES 115
6 ABBREVIATIONS AND GLOSSARY 149
7 ACKNOWLEDGEMENTS 159
8 CURRICULUM VITAE 160
9 PUBLICATIONS WITH DEFINITION OF OWN CONTRIBUTIONS 165
9.1 RADIATION EXPOSURE DURING SPACE MISSIONS 165
9.2 THE SPACE EXPERIMENT CELLRAD (FORMERLY CERASP) 165
9.3 DEVELOPMENT AND EVALUATION OF TEST SYSTEMS 165
9 . 4 N F - K B ACTIVATION BY DIFFERENT RADIATION QUALITIES 1 6 6
10 PUBLICATIONS 169
10.1 PUBLICATION 1 171
10.2 PUBLICATION II 183
10.3 PUBLICATION III 195
10.4 PUBLICATION IV 205
10.5 PUBLICATION V 247
10.6 PUBLICATION VI 259
10.7 PUBLICATION VII 269
10.8 PUBLICATION VIII 285
10.9 PUBLICATION IX 299
10.10 PUBLICATION X 311
10.11 PUBLICATION XI 323
10.12 PUBLICATION XII 329
10.13 PUBLICATION XIII 339
11 APPENDIX 351
11.1 ABSTRACTS OF THE ARTICLES DESCRIBING THE DEVELOPMENT OF THE N F - K
B REPORTER CELL LINE 351
11.1.1 SUITABILITY OF ENHANCED GREEN FLUORESCENT PROTEIN AS A REPORTER
COMPONENT FOR BIOASSAYS 351
11.1.2 ENHANCED GREEN FLUORESCENT PROTEIN AS REPORTER PROTEIN FOR
BIOMONITORING OF CYTOTOXIC EFFECTS IN MAMMALIAN CELLS 352
11.1.3 GENERATION OF STABLY TRANSFECTED MAMMALIAN CELL LINES AS
FLUORESCENT SCREENING
ASSAY FOR N F - K B ACTIVATION-DEPENDENT GENE EXPRESSION 3 5 3
6
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| spelling | Hellweg, Christine E. 1971- Verfasser (DE-588)123502578 aut Cellular response to exposure with different radiation qualities with a focus on nuclear factor kappa-B eingereicht von Christine E. Hellweg 2012 353 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Berlin, Freie Univ., Habil.-Schr. radiation cabt cells cabt cytotoxicity cabt radiotherapy cabt ultraviolet radiation cabt ions cabt (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025500707&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Hellweg, Christine E. 1971- Cellular response to exposure with different radiation qualities with a focus on nuclear factor kappa-B radiation cabt cells cabt cytotoxicity cabt radiotherapy cabt ultraviolet radiation cabt ions cabt |
| subject_GND | (DE-588)4113937-9 |
| title | Cellular response to exposure with different radiation qualities with a focus on nuclear factor kappa-B |
| title_auth | Cellular response to exposure with different radiation qualities with a focus on nuclear factor kappa-B |
| title_exact_search | Cellular response to exposure with different radiation qualities with a focus on nuclear factor kappa-B |
| title_full | Cellular response to exposure with different radiation qualities with a focus on nuclear factor kappa-B eingereicht von Christine E. Hellweg |
| title_fullStr | Cellular response to exposure with different radiation qualities with a focus on nuclear factor kappa-B eingereicht von Christine E. Hellweg |
| title_full_unstemmed | Cellular response to exposure with different radiation qualities with a focus on nuclear factor kappa-B eingereicht von Christine E. Hellweg |
| title_short | Cellular response to exposure with different radiation qualities with a focus on nuclear factor kappa-B |
| title_sort | cellular response to exposure with different radiation qualities with a focus on nuclear factor kappa b |
| topic | radiation cabt cells cabt cytotoxicity cabt radiotherapy cabt ultraviolet radiation cabt ions cabt |
| topic_facet | radiation cells cytotoxicity radiotherapy ultraviolet radiation ions Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025500707&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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