Generation of a neonatal sepsis model in humanized mice:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online-Zugang: | Volltext https://nbn-resolving.org/urn:nbn:de:bvb:355-epub-271745 Inhaltsverzeichnis |
| Beschreibung: | V, 139 S. Ill., graph. Darst. |
Internformat
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| 245 | 1 | 0 | |a Generation of a neonatal sepsis model in humanized mice |c vorgelegt von Wolfgang Ernst |
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| 300 | |a V, 139 S. |b Ill., graph. Darst. | ||
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| 337 | |b n |2 rdamedia | ||
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| 650 | 0 | 7 | |a Sepsis |0 (DE-588)4181014-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Tiermodell |0 (DE-588)4140660-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Streptococcus agalactiae |0 (DE-588)4340809-6 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Streptococcus agalactiae |0 (DE-588)4340809-6 |D s |
| 689 | 0 | 1 | |a Sepsis |0 (DE-588)4181014-4 |D s |
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Datensatz im Suchindex
| _version_ | 1804149761942487040 |
|---|---|
| adam_text | IMAGE 1
TABLE OF CONTENTS I
TABLE OF CONTENTS
TABLE OF CONTENTS I
ZUSAMMENFASSUNG IV
ABSTRACT V
1 INTRODUCTION 1
1.1 STREPTOCOCCUS AGALACTIAE 1
1.1.1 BACKGROUND AND DISEASE 1
1.1.2 VIRULENCE FACTORS OF GBS THAT FACILITATE UPTAKE OF PATHOGEN 3
1.1.3 VIRULENCE FACTORS OF GBS FOR IMMUNE EVASION 6
1.2 NEONATAL IMMUNE SYSTEM 8
1.2.1 T CELLS 9
1.2.2 B CELLS 10
1.2.3 MONOCYTES/MACROPHAGES 12
1.2.4 DENDRITIC CELLS 13
1.2.5 NEUTROPHILS 14
1.3 HUMANIZED MOUSE MODEL 16
1.4 BACKGROUND ON TREATMENT OPTIONS 25
1.4.1 LUNG MATURATION - BETAMETHASONE 25
1.4.2 TOCOLYSIS - INDOMETHACIN 26
1.5 AIM 28
2 MATERIAL & METHODS 29
2.1 METHODS 29
2.1.1 ANIMALS 29
2.1.2 HUMANIZATION OF MICE 29
2.1.3 GROWING AND QUANTIFYING BACTERIA FOR INFECTION 30
2.1.4 INFECTION AND TREATMENT OF ANIMALS 31
HTTP://D-NB.INFO/1030580782
IMAGE 2
TABLE OF CONTENTS II
2.1.5 PREPARATION OF BLOOD, SINGE CELL SUSPENSIONS AND TISSUE FROM MICE
32
2.1.6 PERCOLL CELL SEPARATION 32
2.1.7 DETECTION OF COLONY FORMING UNITS IN VARIOUS ORGANS 33
2.1.8 STAINING FOR FLOW CYTOMETRY 33
2.1.9 FREEZING OF MNC 34
2.1.10 IN VITRO DIFFERENTIATION OF MONOCYTES AND SUBSEQUENT STIMULATION
35
2.1.11 RESTIMULATION OF PEC FROM INFECTED HUMANIZED MICE 35
2.1.12 CLEARANCE AND SURVIVAL OF CORD-BLOOD DERIVED MNC 36
2.1.13 PREPARATION AND SECTIONING OF PARAFFIN-EMBEDDED TISSUE SAMPLES 36
2.1.14 IMMUNOHISTOCHEMISTRY OF PARAFFIN SECTIONS 37
2.1.15 HEMATOXYLIN & EOSIN STAINING 38
2.1.16 CYTOSPIN AND STAINING OF PEC 38
2.1.17 ASSAY FOR MEASURING NITRITE ACCUMULATION (NO PRODUCTION) 39
2.1.18 CYTOKINE DETECTION 39
2.2 MATERIALS 40
3 RESULTS 48
3.1 GENERATION AND CHARACTERIZATION OF HUMANIZED MICE 48
3.2 INFECTION MODEL 54
3.2.1 CHARACTERIZATION OF GBS INFECTION IN HUMANIZED MICE 54
3.2.2 DISEASE PROGRESSION IN GBS INFECTED HUMANIZED MICE 56
3.2.3 CHARACTERIZATION OF ORGAN DAMAGE AFTER GBS INFECTION 57
3.2.4 EFFECT OF INFECTION ON CELL COUNT 58
3.2.5 RESPONSE OF THE HUMAN IMMUNE SYSTEM - MIGRATION AND
PROLIFERATION.... 60
3.2.6 RESPONSE OF THE HUMAN IMMUNE SYSTEM - CYTOKINE PRODUCTION 64
3.3 EFFECT OF TREATMENT ON DISEASE PROGRESSION 67
3.3.1 TREATMENT REGIMEN 67
3.3.2 IMPACT OF TREATMENT ON CLEARANCE OF BACTERIA 68
3.3.3 EFFECT OF TREATMENT ON TOTAL CELL COUNT 70
IMAGE 3
TABLE OF CONTENTS III
3.3.4 EFFECT OF TREATMENT ON MIGRATION AND PROLIFERATION OF LEUKOCYTES
72
3.3.5 EFFECT OF TREATMENT ON HUMAN IMMUNE RESPONSE - CYTOKINE
PRODUCTION... 80
3.3.6 EFFECT OF TREATMENT ON HUMAN IMMUNE RESPONSE - NO PRODUCTION 81
3.3.7 EFFECT OF TREATMENT ON DISEASE PROGRESSION - ORGAN DAMAGE 81
3.4 EFFECTS OF TREATMENT IN VITRO 83
4 DISCUSSION 86
4.1 HUMANIZED MICE AS A GBS INFECTION MODEL 86
4.2 EFFECTS OF GBS INFECTION IN HUMANIZED MICE 88
4.2.2 ORGAN DAMAGE 88
4.2.3 CHANGES IN LEUKOCYTE POPULATIONS AND CYTOKINE PROFILE 89
4.3 EFFECTS OF TREATMENT ON AN ONGOING GBS INFECTION IN HUMANIZED MICE
94
4.3.1 EFFECTS OF BETAMETHASONE TREATMENT 94
4.3.2 EFFECTS OF INDOMETHACIN TREATMENT 96
4.4 EFFECTS OF TREATMENT ON HUMAN CORD BLOOD DERIVED MNC IN VITRO 98
5 SUMMARY & OUTLOOK 100
REFERENCES 101
SUPPLEMENT 122
ABBREVIATIONS 128
LIST OF TABLES 134
LIST OF FIGURES 135
APPENDIX 137
PRESENTATIONS 137
PUBLICATIONS 138
AWARDS AND SCHOLARSHIPS 138
ACKNOWLEDGMENTS 139
|
| any_adam_object | 1 |
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| discipline | Medizin |
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| spelling | Ernst, Wolfgang Verfasser aut Generation of a neonatal sepsis model in humanized mice vorgelegt von Wolfgang Ernst 2012 V, 139 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Regensburg, Univ., Diss., 2012 Sepsis (DE-588)4181014-4 gnd rswk-swf Tiermodell (DE-588)4140660-6 gnd rswk-swf Streptococcus agalactiae (DE-588)4340809-6 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Streptococcus agalactiae (DE-588)4340809-6 s Sepsis (DE-588)4181014-4 s Tiermodell (DE-588)4140660-6 s b DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:355-epub-271745 http://epub.uni-regensburg.de/27174/ Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:355-epub-271745 Resolving-System DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025471891&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Ernst, Wolfgang Generation of a neonatal sepsis model in humanized mice Sepsis (DE-588)4181014-4 gnd Tiermodell (DE-588)4140660-6 gnd Streptococcus agalactiae (DE-588)4340809-6 gnd |
| subject_GND | (DE-588)4181014-4 (DE-588)4140660-6 (DE-588)4340809-6 (DE-588)4113937-9 |
| title | Generation of a neonatal sepsis model in humanized mice |
| title_auth | Generation of a neonatal sepsis model in humanized mice |
| title_exact_search | Generation of a neonatal sepsis model in humanized mice |
| title_full | Generation of a neonatal sepsis model in humanized mice vorgelegt von Wolfgang Ernst |
| title_fullStr | Generation of a neonatal sepsis model in humanized mice vorgelegt von Wolfgang Ernst |
| title_full_unstemmed | Generation of a neonatal sepsis model in humanized mice vorgelegt von Wolfgang Ernst |
| title_short | Generation of a neonatal sepsis model in humanized mice |
| title_sort | generation of a neonatal sepsis model in humanized mice |
| topic | Sepsis (DE-588)4181014-4 gnd Tiermodell (DE-588)4140660-6 gnd Streptococcus agalactiae (DE-588)4340809-6 gnd |
| topic_facet | Sepsis Tiermodell Streptococcus agalactiae Hochschulschrift |
| url | http://epub.uni-regensburg.de/27174/ https://nbn-resolving.org/urn:nbn:de:bvb:355-epub-271745 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025471891&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT ernstwolfgang generationofaneonatalsepsismodelinhumanizedmice |