Tyrosine residues Y114 and Y127 of rhadinoviral oncoprotein Tip are central to STAT activation: = Die Tyrosinreste Y114 und Y127 des rhadinoviralen Onkoproteins Tip sind essentiell für die STAT-Aktivierung
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2012
|
| Schlagworte: | |
| Online-Zugang: | Volltext https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-34994 http://d-nb.info/1027220479/34 Inhaltsverzeichnis |
| Beschreibung: | 55 S. Ill., graph. Darst. |
Internformat
MARC
| LEADER | 00000nam a2200000zc 4500 | ||
|---|---|---|---|
| 001 | BV040601286 | ||
| 003 | DE-604 | ||
| 005 | 20210311 | ||
| 007 | t | ||
| 008 | 121130s2012 gw ad|| m||| 00||| eng d | ||
| 015 | |a 12,O11 |2 dnb | ||
| 016 | 7 | |a 1027220479 |2 DE-101 | |
| 035 | |a (OCoLC)820435212 | ||
| 035 | |a (DE-599)DNB1027220479 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c XA-DE-BY | ||
| 049 | |a DE-384 |a DE-473 |a DE-703 |a DE-1051 |a DE-824 |a DE-29 |a DE-12 |a DE-91 |a DE-19 |a DE-1049 |a DE-92 |a DE-739 |a DE-898 |a DE-355 |a DE-706 |a DE-20 |a DE-1102 |a DE-29T | ||
| 082 | 0 | |a 570 | |
| 082 | 0 | |a 610 | |
| 084 | |a 610 |2 sdnb | ||
| 100 | 1 | |a Mazumder, Eman Dey |d 1977- |e Verfasser |0 (DE-588)1026157471 |4 aut | |
| 245 | 1 | 0 | |a Tyrosine residues Y114 and Y127 of rhadinoviral oncoprotein Tip are central to STAT activation |b = Die Tyrosinreste Y114 und Y127 des rhadinoviralen Onkoproteins Tip sind essentiell für die STAT-Aktivierung |c von Eman Dey Mazumder |
| 246 | 1 | 1 | |a Die Tyrosinreste Y114 und Y127 des rhadinoviralen Onkoproteins Tip sind essentiell für die STAT-Aktivierung |
| 264 | 1 | |c 2012 | |
| 300 | |a 55 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Erlangen-Nürnberg, Univ., Diss., 2012 | ||
| 650 | 0 | 7 | |a STAT |0 (DE-588)4790744-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a T-Lymphozyt |0 (DE-588)4127387-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Herpesvirus saimiri |0 (DE-588)4159628-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Signaltransduktion |0 (DE-588)4318717-1 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Herpesvirus saimiri |0 (DE-588)4159628-6 |D s |
| 689 | 0 | 1 | |a STAT |0 (DE-588)4790744-7 |D s |
| 689 | 0 | 2 | |a T-Lymphozyt |0 (DE-588)4127387-4 |D s |
| 689 | 0 | 3 | |a Signaltransduktion |0 (DE-588)4318717-1 |D s |
| 689 | 0 | |5 DE-604 | |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |o urn:nbn:de:bvb:29-opus-34994 |
| 856 | 4 | |u https://open.fau.de/handle/openfau/2441 |x Verlag |z kostenfrei |3 Volltext | |
| 856 | 4 | |u https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-34994 |x Resolving-System | |
| 856 | 4 | |u http://d-nb.info/1027220479/34 |x Langzeitarchivierung Nationalbibliothek | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025429033&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 912 | |a ebook | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-025429033 | ||
Datensatz im Suchindex
| _version_ | 1804149691424702465 |
|---|---|
| adam_text | IMAGE 1
C O N T E N T S
I. ABSTRACT 1
1. ABSTRACT 1
2. ZUSAMMENFASSUNG IN DEUTSCHER SPRACHE 2
N . INTRODUCTION 3
III. RATIONALE 11
IV. MATERIALS AND METHODS 12
1. MATERIALS 12
1.1 BACTERIA STRAINS FOR PLASMID ISOLATION 12
1.2 CELL LINES 12
1.3 MEDIA 12
1.4 OLIGONUCLEOTIDES 13
1.5 ANTIBODIES 13
1.6 BUFFERS AND SOLUTIONS 14
2. METHODS 15
2.1 CULTIVATION O F BACTERIA FOR PLASMID PREPARATION 15
2.2 NUCLEIC ACID METHODS 15
2.3 CELL CULTURE 16
2.4 TRANSFECTION 16
2.5 ELECTROPORATION 16
2.6 CELL LYSIS FOR IMMUNOBLOTTING 17
2.7 WESTERN BLOT/SDS POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE) 17
2.8 IMMUNOFLUORESCENCE 17
2.9 LUCIFERASE REPORTER ASSAY 18
2.10 STREP-TAG N / STREP-TACTIN CO-IMMUNOPRECIPITATION SYSTEM 18
2.11 ACCEPTOR PHOTOBLEACHING WITH AUTOFLOURESCENT PROTEINS 18
IV. RESULTS 2 0
1. STAT5 PHOSPHORYLATION DEPENDS ON Y127 20
2. TIP CO-PRECIPITATES BOTH STAT5 AND LCK 21
3. TYROSINE RESIDUE Y127 O F TIP SERVES AS A DOCKING SITE FOR STAT5
RECRUITMENT 22
4. STAT5 PHOSPHORYLATION IN THE PRESENCE O F TIP AND LCK IS INDEPENDENT
O F THE JANUS KINASES 23
5. WILD TYPE TIP AND STAT5 CAN INTERACT INDEPENDENTLY 24
6. PROPOSED SEQUENTIAL BINDING O F TIP AND STAT5 TO LCK DURING THE
COMPLEX FORMATION. ..26
7. LOCALIZATION O F TIP AND STAT5 28
8. WILDTYPE YPET-TIP AND STAT5-CYPET MEET AT THE CELL MEMBRANE TO
UNDERGO FRET ....31
9. CONTROL CONSTRUCTS MONITORING TRUE FRET 33
10. NEGATIVE CONTROLS TO RULE OUT PSEUDO-FRET 35
11. DIFFERENTIAL ROLE O F THE TYROSINES 114 AND 127 IN STAT3 RECOGNITION
36
12. TIP TYROSINE RESIDUES Y114 AND Y127 IN THE RECOGNITION AND BINDING O
F STAT6 37
13. TIP-Y127 DRIVEN STAT6 ACTIVATION 38
14. LCK IS REQUIRED FOR STAT6 ACTIVATION 39
V. DISCUSSION 4 2
VI. REFERENCES 48
HTTP://D-NB.INFO/1026905524
|
| any_adam_object | 1 |
| author | Mazumder, Eman Dey 1977- |
| author_GND | (DE-588)1026157471 |
| author_facet | Mazumder, Eman Dey 1977- |
| author_role | aut |
| author_sort | Mazumder, Eman Dey 1977- |
| author_variant | e d m ed edm |
| building | Verbundindex |
| bvnumber | BV040601286 |
| collection | ebook |
| ctrlnum | (OCoLC)820435212 (DE-599)DNB1027220479 |
| dewey-full | 570 610 |
| dewey-hundreds | 500 - Natural sciences and mathematics 600 - Technology (Applied sciences) |
| dewey-ones | 570 - Biology 610 - Medicine and health |
| dewey-raw | 570 610 |
| dewey-search | 570 610 |
| dewey-sort | 3570 |
| dewey-tens | 570 - Biology 610 - Medicine and health |
| discipline | Biologie Medizin |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>02489nam a2200529zc 4500</leader><controlfield tag="001">BV040601286</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20210311 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">121130s2012 gw ad|| m||| 00||| eng d</controlfield><datafield tag="015" ind1=" " ind2=" "><subfield code="a">12,O11</subfield><subfield code="2">dnb</subfield></datafield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">1027220479</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)820435212</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DNB1027220479</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakddb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">XA-DE-BY</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-384</subfield><subfield code="a">DE-473</subfield><subfield code="a">DE-703</subfield><subfield code="a">DE-1051</subfield><subfield code="a">DE-824</subfield><subfield code="a">DE-29</subfield><subfield code="a">DE-12</subfield><subfield code="a">DE-91</subfield><subfield code="a">DE-19</subfield><subfield code="a">DE-1049</subfield><subfield code="a">DE-92</subfield><subfield code="a">DE-739</subfield><subfield code="a">DE-898</subfield><subfield code="a">DE-355</subfield><subfield code="a">DE-706</subfield><subfield code="a">DE-20</subfield><subfield code="a">DE-1102</subfield><subfield code="a">DE-29T</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">570</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">610</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">610</subfield><subfield code="2">sdnb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Mazumder, Eman Dey</subfield><subfield code="d">1977-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)1026157471</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Tyrosine residues Y114 and Y127 of rhadinoviral oncoprotein Tip are central to STAT activation</subfield><subfield code="b">= Die Tyrosinreste Y114 und Y127 des rhadinoviralen Onkoproteins Tip sind essentiell für die STAT-Aktivierung</subfield><subfield code="c">von Eman Dey Mazumder</subfield></datafield><datafield tag="246" ind1="1" ind2="1"><subfield code="a">Die Tyrosinreste Y114 und Y127 des rhadinoviralen Onkoproteins Tip sind essentiell für die STAT-Aktivierung</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2012</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">55 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">Erlangen-Nürnberg, Univ., Diss., 2012</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">STAT</subfield><subfield code="0">(DE-588)4790744-7</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">T-Lymphozyt</subfield><subfield code="0">(DE-588)4127387-4</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Herpesvirus saimiri</subfield><subfield code="0">(DE-588)4159628-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Signaltransduktion</subfield><subfield code="0">(DE-588)4318717-1</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Herpesvirus saimiri</subfield><subfield code="0">(DE-588)4159628-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">STAT</subfield><subfield code="0">(DE-588)4790744-7</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">T-Lymphozyt</subfield><subfield code="0">(DE-588)4127387-4</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="3"><subfield code="a">Signaltransduktion</subfield><subfield code="0">(DE-588)4318717-1</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Online-Ausgabe</subfield><subfield code="o">urn:nbn:de:bvb:29-opus-34994</subfield></datafield><datafield tag="856" ind1="4" ind2=" "><subfield code="u">https://open.fau.de/handle/openfau/2441</subfield><subfield code="x">Verlag</subfield><subfield code="z">kostenfrei</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="856" ind1="4" ind2=" "><subfield code="u">https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-34994</subfield><subfield code="x">Resolving-System</subfield></datafield><datafield tag="856" ind1="4" ind2=" "><subfield code="u">http://d-nb.info/1027220479/34</subfield><subfield code="x">Langzeitarchivierung Nationalbibliothek</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025429033&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ebook</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-025429033</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV040601286 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T00:27:03Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-025429033 |
| oclc_num | 820435212 |
| open_access_boolean | 1 |
| owner | DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 DE-29T |
| owner_facet | DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 DE-29T |
| physical | 55 S. Ill., graph. Darst. |
| psigel | ebook |
| publishDate | 2012 |
| publishDateSearch | 2012 |
| publishDateSort | 2012 |
| record_format | marc |
| spelling | Mazumder, Eman Dey 1977- Verfasser (DE-588)1026157471 aut Tyrosine residues Y114 and Y127 of rhadinoviral oncoprotein Tip are central to STAT activation = Die Tyrosinreste Y114 und Y127 des rhadinoviralen Onkoproteins Tip sind essentiell für die STAT-Aktivierung von Eman Dey Mazumder Die Tyrosinreste Y114 und Y127 des rhadinoviralen Onkoproteins Tip sind essentiell für die STAT-Aktivierung 2012 55 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Erlangen-Nürnberg, Univ., Diss., 2012 STAT (DE-588)4790744-7 gnd rswk-swf T-Lymphozyt (DE-588)4127387-4 gnd rswk-swf Herpesvirus saimiri (DE-588)4159628-6 gnd rswk-swf Signaltransduktion (DE-588)4318717-1 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Herpesvirus saimiri (DE-588)4159628-6 s STAT (DE-588)4790744-7 s T-Lymphozyt (DE-588)4127387-4 s Signaltransduktion (DE-588)4318717-1 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:29-opus-34994 https://open.fau.de/handle/openfau/2441 Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-34994 Resolving-System http://d-nb.info/1027220479/34 Langzeitarchivierung Nationalbibliothek DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025429033&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Mazumder, Eman Dey 1977- Tyrosine residues Y114 and Y127 of rhadinoviral oncoprotein Tip are central to STAT activation = Die Tyrosinreste Y114 und Y127 des rhadinoviralen Onkoproteins Tip sind essentiell für die STAT-Aktivierung STAT (DE-588)4790744-7 gnd T-Lymphozyt (DE-588)4127387-4 gnd Herpesvirus saimiri (DE-588)4159628-6 gnd Signaltransduktion (DE-588)4318717-1 gnd |
| subject_GND | (DE-588)4790744-7 (DE-588)4127387-4 (DE-588)4159628-6 (DE-588)4318717-1 (DE-588)4113937-9 |
| title | Tyrosine residues Y114 and Y127 of rhadinoviral oncoprotein Tip are central to STAT activation = Die Tyrosinreste Y114 und Y127 des rhadinoviralen Onkoproteins Tip sind essentiell für die STAT-Aktivierung |
| title_alt | Die Tyrosinreste Y114 und Y127 des rhadinoviralen Onkoproteins Tip sind essentiell für die STAT-Aktivierung |
| title_auth | Tyrosine residues Y114 and Y127 of rhadinoviral oncoprotein Tip are central to STAT activation = Die Tyrosinreste Y114 und Y127 des rhadinoviralen Onkoproteins Tip sind essentiell für die STAT-Aktivierung |
| title_exact_search | Tyrosine residues Y114 and Y127 of rhadinoviral oncoprotein Tip are central to STAT activation = Die Tyrosinreste Y114 und Y127 des rhadinoviralen Onkoproteins Tip sind essentiell für die STAT-Aktivierung |
| title_full | Tyrosine residues Y114 and Y127 of rhadinoviral oncoprotein Tip are central to STAT activation = Die Tyrosinreste Y114 und Y127 des rhadinoviralen Onkoproteins Tip sind essentiell für die STAT-Aktivierung von Eman Dey Mazumder |
| title_fullStr | Tyrosine residues Y114 and Y127 of rhadinoviral oncoprotein Tip are central to STAT activation = Die Tyrosinreste Y114 und Y127 des rhadinoviralen Onkoproteins Tip sind essentiell für die STAT-Aktivierung von Eman Dey Mazumder |
| title_full_unstemmed | Tyrosine residues Y114 and Y127 of rhadinoviral oncoprotein Tip are central to STAT activation = Die Tyrosinreste Y114 und Y127 des rhadinoviralen Onkoproteins Tip sind essentiell für die STAT-Aktivierung von Eman Dey Mazumder |
| title_short | Tyrosine residues Y114 and Y127 of rhadinoviral oncoprotein Tip are central to STAT activation |
| title_sort | tyrosine residues y114 and y127 of rhadinoviral oncoprotein tip are central to stat activation die tyrosinreste y114 und y127 des rhadinoviralen onkoproteins tip sind essentiell fur die stat aktivierung |
| title_sub | = Die Tyrosinreste Y114 und Y127 des rhadinoviralen Onkoproteins Tip sind essentiell für die STAT-Aktivierung |
| topic | STAT (DE-588)4790744-7 gnd T-Lymphozyt (DE-588)4127387-4 gnd Herpesvirus saimiri (DE-588)4159628-6 gnd Signaltransduktion (DE-588)4318717-1 gnd |
| topic_facet | STAT T-Lymphozyt Herpesvirus saimiri Signaltransduktion Hochschulschrift |
| url | https://open.fau.de/handle/openfau/2441 https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-34994 http://d-nb.info/1027220479/34 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025429033&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT mazumderemandey tyrosineresiduesy114andy127ofrhadinoviraloncoproteintiparecentraltostatactivationdietyrosinrestey114undy127desrhadinoviralenonkoproteinstipsindessentiellfurdiestataktivierung AT mazumderemandey dietyrosinrestey114undy127desrhadinoviralenonkoproteinstipsindessentiellfurdiestataktivierung |