Yeast protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2010
|
| Ausgabe: | 2. ed. |
| Schriftenreihe: | Methods in molecular biology
313 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Originally published in 2006 Includes bibliographical references and index |
| Beschreibung: | xi, 392 S. Ill., graph. Darst. |
| ISBN: | 1617375691 9781617375699 |
Internformat
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| 490 | 1 | |a Methods in molecular biology |v 313 | |
| 500 | |a Originally published in 2006 | ||
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Datensatz im Suchindex
| _version_ | 1804149631292014593 |
|---|---|
| adam_text | Contents
Preface
..............................................................................................................
v
Contributors
.....................................................................................................
¡χ
1
Basic Investigations in Saccharomyces cerevisiae
Brendan P. G.
Curran
and Virginia Bugeja
........................................... 1
2
Isolation of Nucleic Acids
Michelle
Hanna
and Wei Xiao
............................................................ 15
3
Purification of Yeast Peroxisomes
Ben Distel
and
Astrid Kragt................................................................21
4
Isolation of Yeast Plasma Membranes
Barry Panaretou and Peter Piper
........................................................27
5
Isolation of Yeast Mitochondria
Chris Meisinger,
Nikolaus Pfanner,
and Kaye
N.
Truscott
.................33
6
Extraction of Yeast Lipids
Roger Schneiter and
Günther Daum..................................................41
7
Two-Dimensional Gel Electrophoresis of Total Yeast Proteins
Hélian
Boucherie
and
Christelle Monribot-Espagne..........................47
8
Pulsed-Field Gel Electrophoresis of Budding Yeast Chromosomes
Laura
Maringele
and David Lydall
......................................................65
9
Analysis of Yeast Lipids
Roger Schneiter and
Günther Daum..................................................75
10
Yeast Fluorescence Microscopy
Jiří Hašek.............................................................................................
85
11
Intracellular Expression of
Recombinant
Antibody Fluorescent
Protein Fusions for Localization of Target Antigens
in Schizosaccharomyces pombe
Michelle A. Alting-Mees, Eddy P. Risseeuw, Enwu Liu,
Michel
Desautels,
William A. Crosby,
and Sean M. Hemmingsen
..............................................................97
12
Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method
R. Daniel Gietz and Robin A. Woods
............................................... 107
13
Mutagenesis
Leslie Barbour, Michelle
Hanna,
and Wei Xiao
............................... 121
VII
v i
Contents
14
Gene
Disruption in the Budding Yeast Saccharomyces cerevisiae
Johannes H.
Hegemann, Ulrich Güldener,
and
Gabriele J. Köhler.................................................................. 129
15
Inducible
Degron
and Its Application to Creating Conditional
Mutants
R.
Jürgen Dohmen............................................................................. 745
16
Synthetic Lethal Screen
Leslie
Barbou
r
and Wei Xiao
............................................................ 161
17
Synthetic Genetic Array Analysis in Saccharomyces cerevisiae
Amy
Hin
Yan
Tong
and Charles Boone
............................................ 171
18
Two-Dimensional
Agarose Gel
Analysis of
DNA
Replication
Intermediates
Alain T. Dandjinou, Michel
Larrivée,
Ralf
E. Wellinger,
and
Raymund
J.
Wellinger
............................................................ 193
19
Chromatin Assembly in a Crude Fraction From Yeast Cells
Karen M. Robinson and Michael
С
Schultz.....................................209
20
Chromatin Immunoprecipitation to Study Protein-DNA
Interactions in Budding Yeast
Elena Ezhkova and William P. Tansey
..............................................225
21
Isolation of Yeast Nuclei and Micrococcal Nuclease Mapping
of Nucleosome Positioning
Zhengjian Zhang and Joseph
С
Reese
..............................................245
22
Study of Transcriptional Regulation Using a Reporter Gene Assay
Yu
Fu
and Wei Xiao
..........................................................................257
23
Assessing Telomeric Phenotypes
Catherine LeBel, Michel
Larrivée,
Amadou Bah, Nancy Laterreur,
Nancy
Lévesque,
and
Raymund
J.
Wellinger
...............................265
24
Controlled Expression of
Recombinant
Genes and Preparation
of Cell-Free Extracts in Yeast
ZhigangWang
...................................................................................317
25
Production of Heterologous Proteins in Yeast With the Aid
of the Hsp1
50Δ
Carrier
Marja
Makarow,
Anna-Liisa Hänninen,
Taina Suntío,
and
Ricardo
Nunes Bastos
............................................................333
26
Yeast Two-Hybrid
System
Screening
R. Daniel Gietz
.................................................................................345
27
Import
of Precursor Proteins Into Isolated Yeast Mitochondria
Nils Wiedemann,
Nikolaus
Manner, and Peter
Rohling...................373
index
................................................. ........385
|
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| bvnumber | BV040540469 |
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| discipline | Biologie |
| edition | 2. ed. |
| format | Book |
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| id | DE-604.BV040540469 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T00:26:06Z |
| institution | BVB |
| isbn | 1617375691 9781617375699 |
| language | English |
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| physical | xi, 392 S. Ill., graph. Darst. |
| publishDate | 2010 |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Yeast protocols ed. by Wei Xiao 2. ed. Totowa, NJ Humana Press 2010 xi, 392 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 313 Originally published in 2006 Includes bibliographical references and index Yeast fungi / Biotechnology / Laboratory manuals Yeast fungi / Laboratory manuals Methode (DE-588)4038971-6 gnd rswk-swf Saccharomycetaceae (DE-588)4131614-9 gnd rswk-swf Biologisches Verfahren (DE-588)4361950-2 gnd rswk-swf Pflanzenphysiologie (DE-588)4045580-4 gnd rswk-swf Hefeartige Pilze (DE-588)4230509-3 gnd rswk-swf Biotechnologie (DE-588)4069491-4 gnd rswk-swf Saccharomyces (DE-588)4178811-4 gnd rswk-swf Cytologie (DE-588)4070177-3 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Hefeartige Pilze (DE-588)4230509-3 s Biotechnologie (DE-588)4069491-4 s Methode (DE-588)4038971-6 s DE-604 Saccharomycetaceae (DE-588)4131614-9 s Cytologie (DE-588)4070177-3 s Biologisches Verfahren (DE-588)4361950-2 s 2\p DE-604 Pflanzenphysiologie (DE-588)4045580-4 s 3\p DE-604 Saccharomyces (DE-588)4178811-4 s 4\p DE-604 Xiao, Wei 1957- Sonstige (DE-588)137120672 oth Erscheint auch als Online-Ausgabe 1-59259-958-3 Erscheint auch als Online-Ausgabe 978-1-59259-958-5 Methods in molecular biology 313 (DE-604)BV035362695 313 Digitalisierung UB Bayreuth application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025386385&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 3\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 4\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Yeast protocols Methods in molecular biology Yeast fungi / Biotechnology / Laboratory manuals Yeast fungi / Laboratory manuals Methode (DE-588)4038971-6 gnd Saccharomycetaceae (DE-588)4131614-9 gnd Biologisches Verfahren (DE-588)4361950-2 gnd Pflanzenphysiologie (DE-588)4045580-4 gnd Hefeartige Pilze (DE-588)4230509-3 gnd Biotechnologie (DE-588)4069491-4 gnd Saccharomyces (DE-588)4178811-4 gnd Cytologie (DE-588)4070177-3 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4131614-9 (DE-588)4361950-2 (DE-588)4045580-4 (DE-588)4230509-3 (DE-588)4069491-4 (DE-588)4178811-4 (DE-588)4070177-3 (DE-588)4143413-4 |
| title | Yeast protocols |
| title_auth | Yeast protocols |
| title_exact_search | Yeast protocols |
| title_full | Yeast protocols ed. by Wei Xiao |
| title_fullStr | Yeast protocols ed. by Wei Xiao |
| title_full_unstemmed | Yeast protocols ed. by Wei Xiao |
| title_short | Yeast protocols |
| title_sort | yeast protocols |
| topic | Yeast fungi / Biotechnology / Laboratory manuals Yeast fungi / Laboratory manuals Methode (DE-588)4038971-6 gnd Saccharomycetaceae (DE-588)4131614-9 gnd Biologisches Verfahren (DE-588)4361950-2 gnd Pflanzenphysiologie (DE-588)4045580-4 gnd Hefeartige Pilze (DE-588)4230509-3 gnd Biotechnologie (DE-588)4069491-4 gnd Saccharomyces (DE-588)4178811-4 gnd Cytologie (DE-588)4070177-3 gnd |
| topic_facet | Yeast fungi / Biotechnology / Laboratory manuals Yeast fungi / Laboratory manuals Methode Saccharomycetaceae Biologisches Verfahren Pflanzenphysiologie Hefeartige Pilze Biotechnologie Saccharomyces Cytologie Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025386385&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT xiaowei yeastprotocols |