Evaluation neuer molekularer Ansätze für die Behandlung der Systemischen Sklerose:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online-Zugang: | Volltext https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-35530 http://d-nb.info/1026527023/34 Inhaltsverzeichnis |
| Beschreibung: | VII, 113 S. Ill., graph. Darst. |
Internformat
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| 245 | 1 | 0 | |a Evaluation neuer molekularer Ansätze für die Behandlung der Systemischen Sklerose |c von Clara Dees |
| 264 | 1 | |c 2012 | |
| 300 | |a VII, 113 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
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| 502 | |a Erlangen-Nürnberg, Univ., Diss., 2012 | ||
| 650 | 0 | 7 | |a Serotonin |0 (DE-588)4054614-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Progressive Sklerodermie |0 (DE-588)4175868-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Fibrose |0 (DE-588)4198241-1 |2 gnd |9 rswk-swf |
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Datensatz im Suchindex
| _version_ | 1804149626816692224 |
|---|---|
| adam_text | IMAGE 1
INDEX O F CONTENTS
INDEX OF CONTENTS
1 S U M M A R Y 1
2 Z U S A M M E N F A S S U N G 3
3 I N T R O D U C T I O N 5
3.1 S Y S T E M I C SCLEROSIS 5
3.1.1 E P I D E M I O L O G Y A N D CLASSIFICATION 5
3.1.2 P A T H O G E N E S I S 5
3.1.2 1 V A S C U L O P A T H Y 6
3.1.2.2 CELLULAR I M M U N E ACTIVATION A N D A U T O I M M U N I T Y 8
3.1.2.3 FIBROSIS 9
3.1.3 A N I M A L NIODCLS O F S Y S T E M I C SCLEROSIS 10
3.1.3.1 T H E M O U S E M O D E L O F BLEOMYCIN-INDUCED DERMAL FIBROSIS
11
3.1.3.2 T H E TIGHT-SKIN 1 (TSK-1) M O U S E M O D E L 12
3.2 .MAMMALIAN SKIN 13
3.3 S I G N A L I N G O F 5 - H Y D R O X Y T R Y P T A M I N E ( 5 - H
T ) 15
3.3.1 S Y N T H E S I S A N D FUNCTION O F 5 - H T 15
3.3.2 R E C E P T O R S AND SIGNALING 17
3.4 T H E N O T C H P A T H W A Y 19
3.4.1 P A T H W A Y C O M P O N E N T S 19
3.4.2 S I G N A L TRANSDUCTION A N D REGULATION 23
4 A1MS O F T H E S T U D Y 2 6
5 R E S U L T S 2 7
5.1 EVALUATION O F T H E 5 - H T SIGNALING P A T H W A Y IN THE D E V E
L O P M E N T O F SKIN FIBROSIS 2 7
5.1.1. CONTRIBUTION O F PLATELETS TO SKIN FIBROSIS 2 7
5.1.1.1 INHIBITION O F PLATELET ACTIVATION IN EXPERIMENTAL FIBROSIS 2 7
5.1.1.2 K N O C K D O W N O F TRYPTOPHAN H Y D R O X Y L A S E 1 ( T P H
- 1 ) 3 0
5.1.2 EFFECTS O F 5 - H T ON D E R M A L FIBROBLASTS A N D M E C H A N I
S M O F ACTION 3 2
5.1.2 .1 STIMULATION O F D E R M A L FIBROBLASTS WITH 5 - H T 3 2
5 1.2.2 INHIBITION O F 5 - H T SIGNALING A N D IDENTIFICATION O F T H E
M E D I A T I N G RECEPTOR.. 3 4
5.1.2.3 D O W N S T R E A M SIGNALING O F 5 - H T 2 B - INTERACTIONS W I
T H T G F SS SIGNALING 3 8
5.1.3 INHIBITION O F 5 - H T SIGNALING IN VIVO 3 9
5.1.3.1 PHARMACOLOGICAL INHIBITION 3 9
5.1.3.2 G E N E T I C APPROACH WITH K N O C K D O W N O F 5 - H T 2 B 4
3
V
HTTP://D-NB.INFO/1026864798
IMAGE 2
INDEX O F CONTENTS
5.2 A N A L Y S I S O F N O T C H SIGNALING IN FIBROBLAST ACTIVATION A N
D TISSUE FIBROSIS 4 7
5.2.1 ACTIVATION O F THE N O T C H P A T H W A Y IN FIBROTIC DISEASE 4 7
5.2.2 STIMULATION A N D INHIBITION O F N O T C H SIGNALING IN VITRO 5 0
5.2.2.1 ACTIVATION O F N O T C H SIGNALING B Y R E C O M B I N A N T J A
G L - F C 5 0
5.2.2.2 BLOCKADE O F N O T C H P A T H W A Y ACTIVATION B Y INHIBITION O
F Y-SECRETASE 5 3
5.2.3 T A R G E T I N G N O T C H SIGNALING IN EXPERIMENTAL FIBROSIS 5 6
5.2.3.1 PREVENTION A N D TREATMENT O F BLEOMYCIN-INDUCED DERMAL FIBROSIS
5 6
5.2.3.2 INHIBITION O F N O T C H SIGNALING IN T S K - I M I C E 6 2
6 D I S C U S S I O N 6 5
6.1 5 - H T 2 B PLAYS A CENTRAL ROLE IN FIBROTIC DISEASE 6 5
6.2 NOTCH SIGNALING CONTROLS FIBROBLAST ACTIVATION A N D TISSUE FIBROSIS
6 8
6.3 C O N C L U S I O N 7 2
7 M A T E R I A L A N D M E T H O D S 7 3
7.1 MATERIAL 7 3
7 .1.1 C H E M I C A L S A N D C O N S U M A B L E S 7 3
7.1.2 PATIENTS 7 3
7.1.3 M I C E 7 4
7.1.4 STIMULANTS, INHIBITORS A N D ANTIBODIES 7 4
7.1.5 P C R P R I M E R S A N D S I R N A 7 5
7. 1.6 BUFFERS A N D SOLUTIONS 7 8
7.2 M E T H O D S 8 0
7.2.1 FIBROBLAST CULTURE 8 0
7.2.2 STIMULATION O F CULTURED FIBROBLASTS 8 0
7.2.3 INHIBITION O F DIFFERENT P A T H W A Y S 81
7.2.3.1 INHIBITION O F 5 - H T SIGNALING 81
7.2.3.2 SELECTIVE INHIBITION O F T G F SS SIGNALING 81
7.2.3 3 BLOCKADE O F T H E N O T C H P A T H W A Y 81
7.2.3.4 NUCLEOFECTION W I T H S I R N A 81
7.2.4 M R N A STABILITY A S S A Y 81
7.2.5 LUCIFERASE R E P O R T E R ASSAY 8 2
7.2.6 M I C R O T I T E R TETRAZOLIUM ( M T T ) A S S A Y 8 2
7.2.7 QUANTITATIVE REAL-TIME P C R 8 2
7.2.8 QUANTIFICATION O F COLLAGEN PROTEIN 8 3
7.2.8.1 SIRCOL A S S A Y F R O M CELL CULTURE SUPERNATANTS 8 3
V I
IMAGE 3
INDEX O F CONTENTS
7.2.8.2 HYDROXYPROLINE A S S A Y FROM SKIN BIOPSIES 8 3
7.2.9 KNZVME-IINKCD IMINUNNSORHCNT A S S A Y ( E L I S A ) 8 3
7.2.10 W E S T E R N BLOT 8 4
7.2.11 I M M U N O H I S T O - AND I M M U N O C Y T O C H E M I S T R Y
8 4
7.2.11.1 IMMUNOHISTOCHEMICAL STAININGS 8 4
7.2.11.2 I M M U N O C Y T O C H E M I S T R Y A N D VISUALIZATION O F
STRESS FIBERS 8 5
7.2.12 A N I M A L M O D E L S 8 6
7.2 12.1 PREVENTION O F B L E O M Y C I N - I N D U C E D DERMAL
FIBROSIS 8 6
7.2.12.2 T R E A T M E N T O F ESTABLISHED B L E O M Y C I N - I N D U C
E D DERMAL FIBROSIS 8 6
7.2.12.3 TIGHT-SKIN 1 M O U S E MODEL 8 7
7.2.13 HISTOLOGICAL ANALYSES 8 7
7.2.13.1 HEMATOXYLIN / EOSIN STAINING 8 7
7.2.13.2 T R I C H R O M E STAINING 8 8
7.2.14 STATISTICS 8 8
8 R E F E R E N C E S 8 9
9 A B B R E V I A T I O N S 104
10 C U R R I C U L U M V I T A E 108
11 A C K N O W L E D G M E N T S 113
V I I
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| spelling | Dees, Clara 1983- Verfasser (DE-588)1026864526 aut Evaluation neuer molekularer Ansätze für die Behandlung der Systemischen Sklerose von Clara Dees 2012 VII, 113 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Erlangen-Nürnberg, Univ., Diss., 2012 Serotonin (DE-588)4054614-7 gnd rswk-swf Progressive Sklerodermie (DE-588)4175868-7 gnd rswk-swf Fibrose (DE-588)4198241-1 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Fibrose (DE-588)4198241-1 s Progressive Sklerodermie (DE-588)4175868-7 s Serotonin (DE-588)4054614-7 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:29-opus-35530 https://open.fau.de/handle/openfau/2388 Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-35530 Resolving-System http://d-nb.info/1026527023/34 Langzeitarchivierung Nationalbibliothek DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025383228&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Dees, Clara 1983- Evaluation neuer molekularer Ansätze für die Behandlung der Systemischen Sklerose Serotonin (DE-588)4054614-7 gnd Progressive Sklerodermie (DE-588)4175868-7 gnd Fibrose (DE-588)4198241-1 gnd |
| subject_GND | (DE-588)4054614-7 (DE-588)4175868-7 (DE-588)4198241-1 (DE-588)4113937-9 |
| title | Evaluation neuer molekularer Ansätze für die Behandlung der Systemischen Sklerose |
| title_auth | Evaluation neuer molekularer Ansätze für die Behandlung der Systemischen Sklerose |
| title_exact_search | Evaluation neuer molekularer Ansätze für die Behandlung der Systemischen Sklerose |
| title_full | Evaluation neuer molekularer Ansätze für die Behandlung der Systemischen Sklerose von Clara Dees |
| title_fullStr | Evaluation neuer molekularer Ansätze für die Behandlung der Systemischen Sklerose von Clara Dees |
| title_full_unstemmed | Evaluation neuer molekularer Ansätze für die Behandlung der Systemischen Sklerose von Clara Dees |
| title_short | Evaluation neuer molekularer Ansätze für die Behandlung der Systemischen Sklerose |
| title_sort | evaluation neuer molekularer ansatze fur die behandlung der systemischen sklerose |
| topic | Serotonin (DE-588)4054614-7 gnd Progressive Sklerodermie (DE-588)4175868-7 gnd Fibrose (DE-588)4198241-1 gnd |
| topic_facet | Serotonin Progressive Sklerodermie Fibrose Hochschulschrift |
| url | https://open.fau.de/handle/openfau/2388 https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-35530 http://d-nb.info/1026527023/34 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025383228&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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