Single-molecule force spectroscopy studies of integrin-mediated cell signaling:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online-Zugang: | kostenfrei kostenfrei Inhaltsverzeichnis |
| Beschreibung: | 133 S. graph. Darst. |
Internformat
MARC
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| 245 | 1 | 0 | |a Single-molecule force spectroscopy studies of integrin-mediated cell signaling |c by Jan Opfer |
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| 912 | |a ebook | ||
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Datensatz im Suchindex
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|---|---|
| adam_text | IMAGE 1
CONTENTS
ZUSAMMENFASSUNG 9
ABSTRACT 1 1
1. INTRODUCTION 13
1.1. ATOMIC FORCE SPECTROSCOPY 13
1.2. PUSHING THE FORCE RESOLUTION LIMIT 15
1.3. INTEGRINS 16
2. NOISE REDUCTION 2 1
2.1. MATERIALS AND METHODS 24
2.1.1. STATIONARY WAVELET TRANSFORM 24
2.1.2. THE RENOIR ALGORITHM 24
2.1.3. AUTOMATIC PARAMETER OPTIMIZATION 29
2.1.4. EVALUATION OF THE SIGNAL RECOVERY PERFORMANCE 30
2.1.5. ESTIMATE OF THE NOISE REDUCTION PERFORMANCE FOR FUNDAMENTAL
SIGNAL FORMS 3 1 2.1.6. ANALYSIS OF THE TETHER ONSET FORCE 34
2.1.7. ANALYSIS OF THE SERS SPECTRA 34
2.1.8. ANALYSIS OF THE OPTICAL TWEEZERS MEASUREMENTS 34
2.2. RESULTS 35
2.2.1. SYNTHETIC DATA 35
2.2.2. EXPERIMENTAL DATA 43
2.2.3. IDEAL FILTER PARAMETERS 46
2.3. DISCUSSION 49
3. STEP DETECTION 5 1
3.1. MATERIALS AND METHODS 52
3.1.1. DETECTION METHOD OF KERSSEMAKERS ET AL 52
3.1.2. MSF ALGORITHM 52
3.1.3. GENERATION O F THE TEST SIGNALS 53
3.1.4. EVALUATION OF THE STEP DETECTION ALGORITHMS 56
3.1.5. CALCULATION OF THE STEP HEIGHTS 57
3.1.6. RECORDING OF THE AFM SPECTRA 57
3.2. RESULTS AND DISCUSSION 57
3.2.1. STEP DETECTION PERFORMANCE 57
3.2.2. DISTRIBUTION OF FALSE-POSITIVES AND -NEGATIVES 63
3.2.3. HEIGHT RESOLUTION 65
3.2.4. REPRODUCTION OF CONTINUOUS HEIGHT DISTRIBUTIONS 69
3.2.5. COMPUTATIONAL COST 71
3.2.6. ANALYSIS OF AFM FORCE SPECTRA 7 1
3.2.7. ANALYSIS OF KINESIN MOTOR EXPERIMENTS 73
3.2.8. PARAMETER OPTIMIZATION 74
4. EFFECT OF SDF-LA ON THE MECHANICS OF THE INTEGRIN A 4 |3I ENVIRONMENT
77
4.1. MATERIALS AND METHODS 77
4.1.1. REAGENTS 77
6
HTTP://D-NB.INFO/1027212263
IMAGE 2
4.1.2. LYMPHOCYTES 77
4.1.3. SUBSTRATE PREPARATION 78
4.1.4. AFM MEASUREMENTS 78
4.1.5. DATA ANALYSIS 79
4.1.6. FACS ANALYSIS 80
4.2. RESULTS 80
4.2.1. VLA-4 EXPRESSION 80
4.2.2. ADHESION RATES 8 1
4.2.3. FORCES 84
4.2.4. STEP POSITIONS 85
4.2.5. LYMPHOCYTE STIFFNESS 86
4.3. DISCUSSION 88
5. ROLE OF THE TRANS-MEMBRANE DOMAINS IN INTEGRIN-MEDIATED ADHESION AND
SIGNALING 93
5.1. MATERIALS AND METHODS 94
5.1.1. IN VITRO SITE-DIRECTED MUTAGENESIS 94
5.1.2. CELL CULTURE 94
5.1.3. ATOMIC FORCE SPECTROSCOPY 94
5.2. RESULTS 94
5.3. DISCUSSION 95
6. CONCLUSIONS 103
7. APPENDIX 105
7.1. CURVALYSER REFERENCE MANUAL 105
7.1.1. BASIC CONFIGURATION 105
7.1.2. PROGRAM EXECUTION 106
7.1.3. BASELINE CORRECTION 107
7.1.4. NOISE REDUCTION 108
7.1.5. CONTACT POINT CALIBRATION 108
7.1.6. STEP DETECTION 109
7.1.7. FITTING PROCEDURES 110
7.1.8. OUTPUT FILES 110
7.1.9. OVERVIEW OF IMPORTANT CONFIGURATION PARAMETERS I L L
7.2. PARAMALYSER REFERENCE MANUAL 115
7.2.1. CONFIGURATION 115
7.2.2. PROGRAM EXECUTION 117
7.2.3. TASKS 118
7.2.4. PARAMETERS 119
REFERENCES 121
PUBLICATIONS 131
ACKNOWLEDGMENT 133
7
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| ctrlnum | (OCoLC)820387782 (DE-599)DNB1026211352 |
| discipline | Physik |
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| indexdate | 2024-07-10T00:25:55Z |
| institution | BVB |
| language | English |
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| physical | 133 S. graph. Darst. |
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| spelling | Opfer, Jan 1980- Verfasser (DE-588)1027360882 aut Single-molecule force spectroscopy studies of integrin-mediated cell signaling by Jan Opfer 2012 133 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Ludwig-Maximilians-Universität, Diss., 2012 (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:19-148393 http://edoc.ub.uni-muenchen.de/14839/ Verlag kostenfrei https://nbn-resolving.org/urn:nbn:de:bvb:19-148393 Resolving-System kostenfrei DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025378182&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Opfer, Jan 1980- Single-molecule force spectroscopy studies of integrin-mediated cell signaling |
| subject_GND | (DE-588)4113937-9 |
| title | Single-molecule force spectroscopy studies of integrin-mediated cell signaling |
| title_auth | Single-molecule force spectroscopy studies of integrin-mediated cell signaling |
| title_exact_search | Single-molecule force spectroscopy studies of integrin-mediated cell signaling |
| title_full | Single-molecule force spectroscopy studies of integrin-mediated cell signaling by Jan Opfer |
| title_fullStr | Single-molecule force spectroscopy studies of integrin-mediated cell signaling by Jan Opfer |
| title_full_unstemmed | Single-molecule force spectroscopy studies of integrin-mediated cell signaling by Jan Opfer |
| title_short | Single-molecule force spectroscopy studies of integrin-mediated cell signaling |
| title_sort | single molecule force spectroscopy studies of integrin mediated cell signaling |
| topic_facet | Hochschulschrift |
| url | http://edoc.ub.uni-muenchen.de/14839/ https://nbn-resolving.org/urn:nbn:de:bvb:19-148393 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025378182&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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