Function of the ARGONAUTE protein ZWILLE in Arabidopsis shoot meristem stem cell maintenance:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Enth. außerdem 1 Sonderabdr. aus: Frontiers in plant science ; 2011 |
| Beschreibung: | 93 Bl. Ill., graph. Darst. 30 cm |
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Datensatz im Suchindex
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| adam_text | IMAGE 1
TABLE OF CONTENTS
TABLE OF CONTENTS
ABSTRACT 1
INTRODUCTION 3
STEM CELL REGULATION IN ARABIDOPSIS THALIANA 3
ROLE O F THE HD-ZIP III GENES IN SAM REGULATION 4
ZLL FUNCTIONS IN STEM CELL MAINTENANCE 5
A I M S OF THE STUDY 7
RESULTS 8
PART I. IDENTIFICATION OF ZWILLE DOWNSTREAM, GENES THROUGH GENE ARRAY
STUDIES 8
I.1 EXPERIMENTAL STRATEGY 8
1.1.1 EXPRESSION PROFILING O F ZLL-1 AND L E R EMBRYOS 8
1.1.2 ASSESSMENT O F ARRAY QUALITY 8
1.1.3 SELECTION O F CANDIDATE GENES 9
1.1.4 STRATEGY F O R CHARACTERIZATION O F THE CANDIDATE GENES 11
PART II. HD-ZIP ///TRANSCRIPTION FACTORS AS ZLL CANDIDATE GENES 13
II.1 THE MIR166B GENE IS EXPRESSED IN THE MERISTEM IN ZLL-1 AND L E R
EMBRYOS 14
11.2 ANALYSIS O F MIR165/166 ACTIVITY IN ZLL-1 EMBRYOS 16
11.3 EXPRESSION O F MIR165A IN UFO O R L I CELLS LEADS T O LOSS O F STEM
CELLS 18
11.4 EXPRESSION O F M I M 1 6 5 / 1 6 6 IN UFO AND L I CELLS DOMAINS IS
SUFFICIENT T O RE-ESTABLISH
T H E STEM CELLS 20
11.5 REVOLUTA IS REQUIRED IN THE STEM CELLS F O R MERISTEM MAINTENANCE
24
11.6 ZLL EXPRESSION IN L I CELLS IS SUFFICIENT T O MAINTAIN T H E S H O
O T MERISTEM STEM
CELLS 26
PART III. ADDITIONAL ZLL CANDIDATE GENES 28
111.1 GENES REGULATED BY MIRNAS 28
111.2 GENES INVOLVED IN DNA METHYLATION 28
HTTP://D-NB.INFO/1025316487
IMAGE 2
TABLE OF CONTENTS
111.3 GENES INVOLVED IN DNA DAMAGE AND PROTEIN DEGRADATION (DDBLB) 33
111.4 GENES INVOLVED IN VASCULAR DEVELOPMENT (ACL5) 34
PART IV. SEARCH FOR ZWILLE MODIFIERS THROUGH AN EMS SUPPRESSOR SCREEN 37
PART V. EMBRYONIC DEVELOPMENT IN ARABIDOPSIS THALIANA: FROM THE ZYGOTE
DIVISION TO THE SHOOT MERISTEM 4 1
DISCUSSION 47
1. GENERAL ASPECTS O F T H E MICROARRAY PERFORMED IN ZLL-1 EMBRYOS 47
2. ZLL REGULATES T H E SAM F R O M SPECIFIC DOMAINS THROUGH MIR165/166
AND REV 48
3. ALTERATION O F RNA-DIRECTED DNAMETHYLATION (RDDM) PATHWAY IN ZLL-1
MUTANTS 55
4. IDENTIFICATION O F D D B L B AS MAJOR ZLL RESPONSIVE GENE 58
5. SUPPRESSOR SCREEN T O IDENTIFY ZLL MODIFIERS 59
(
M A T E R I A L A N D M E T H O D S 6 1
MATERIALS 6 1
PLANT MATERIAL 6 1
. TRANSGENES 6 1
CHEMICAL, ENZYMES AND KITS 65
BUFFERS AND MEDIA 65
BACTERIA STRAINS 65
METHODS 66
PLANT W O R K 66
SEED STERILIZATION 66
PLANT TRANSFORMATION 66
SELECTION O F TRANSGENIC PLANT 66
MICROSCOPY AND IMAGE ANALYSIS 67
MICROARRAY EXPERIMENT 67
QUANTITATIVE REVERSE TRANSCRIPTION AND REAL T I M E PCR 69
EMS MUTAGENESIS 69
IMAGE 3
TABLE OF CONTENTS
REFERENCES 70
ABBREVIATIONS 76
APPENDICES 77
APPENDIX 1. GENES UPREGULATED MORE THAN 2 FOLD IN TRANSITION STAGE IN
ZLL-1 COMPARED T O LER
W T AND T H E I R EXPRESSION IN HEART STAGE 77
APPENDIX 2. GENES DOWNREGULATED MORE THAN 2 FOLD IN TRANSITION STAGE IN
ZLL-1 COMPARED T O
LER W T AND T H E I R EXPRESSION IN HEART STAGE 78
APPENDIX 3. GENES UPREGULATED MORE THAN 2 FOLD IN HEART STAGE IN ZLL-1
COMPARED T O LER W T
AND T H E I R EXPRESSION IN TRANSITION STAGE 79
APPENDIX 4. GENES DOWNREGULATED MORE THAN 2 FOLD IN HEART STAGE IN ZLL-1
COMPARED T O LER
W T AND T H E I R EXPRESSION IN TRANSITION STAGE 80
APPENDIX 5. COMMON GENES CHANGED IN TRANSITION AND HEART EMBRYO STAGES,
IN ZLL-1
EXPRESSION T O LER W T 8 1
APPENDIX 6. SUMMARY O F THE TISSUE SPECIFIC ANALYSIS O F M I M 1 6 5 / 1
6 6 , MIR165A, R-REV AND
ZLL 82
APPENDIX 7. EFFECT O F T H E OPERATOR LINES IN MERISTEM FORMATION IN
ZLL-1 MUTANTS 83
APPENDIX 8. FREQUENCY O F SEEDLINGS W I T H NO MERISTEM IN T H E GENETIC
INTERACTION BETWEEN
ZLL AND T H E CANDIDATE GENES 84
APPENDIX 9. CONFIRMATION O F T H E POSITION O F THE INSERTION LINES IN
SEVERAL CANDIDATE GENES BY
SEQUENCING 85
APPENDIX 10. LIST O F CLONES USED IN THE THESIS 86
APPENDIX 11. LIST O F PRIMERS USED IN T H E THESIS 88
APPENDIX 12. LIST O F TRANSGENIC LINES ANALYSED IN THE THESIS 90
APPENDIX 13. LIST O F LINES SELECTED F O R MIM165/166, R-REV AND MIR165A
MISEXPRESSION
STUDIES 92
A C K N O W L E D G E M E N T S 9 3
|
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| author | Boscá San Jose, Sonia |
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| spelling | Boscá San Jose, Sonia Verfasser (DE-588)1025316010 aut Function of the ARGONAUTE protein ZWILLE in Arabidopsis shoot meristem stem cell maintenance vorgelegt von Sonia Boscá San Jose 2012 93 Bl. Ill., graph. Darst. 30 cm txt rdacontent n rdamedia nc rdacarrier Enth. außerdem 1 Sonderabdr. aus: Frontiers in plant science ; 2011 Freiburg (Breisgau), Univ., Diss., 2012 Embryonalentwicklung (DE-588)4070792-1 gnd rswk-swf Apikalmeristem (DE-588)4271341-9 gnd rswk-swf Proteine (DE-588)4076388-2 gnd rswk-swf Stammzelle (DE-588)4665329-6 gnd rswk-swf Ackerschmalwand (DE-588)4141299-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Ackerschmalwand (DE-588)4141299-0 s Apikalmeristem (DE-588)4271341-9 s Stammzelle (DE-588)4665329-6 s Embryonalentwicklung (DE-588)4070792-1 s Proteine (DE-588)4076388-2 s 1\p DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025368360&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Boscá San Jose, Sonia Function of the ARGONAUTE protein ZWILLE in Arabidopsis shoot meristem stem cell maintenance Embryonalentwicklung (DE-588)4070792-1 gnd Apikalmeristem (DE-588)4271341-9 gnd Proteine (DE-588)4076388-2 gnd Stammzelle (DE-588)4665329-6 gnd Ackerschmalwand (DE-588)4141299-0 gnd |
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| title | Function of the ARGONAUTE protein ZWILLE in Arabidopsis shoot meristem stem cell maintenance |
| title_auth | Function of the ARGONAUTE protein ZWILLE in Arabidopsis shoot meristem stem cell maintenance |
| title_exact_search | Function of the ARGONAUTE protein ZWILLE in Arabidopsis shoot meristem stem cell maintenance |
| title_full | Function of the ARGONAUTE protein ZWILLE in Arabidopsis shoot meristem stem cell maintenance vorgelegt von Sonia Boscá San Jose |
| title_fullStr | Function of the ARGONAUTE protein ZWILLE in Arabidopsis shoot meristem stem cell maintenance vorgelegt von Sonia Boscá San Jose |
| title_full_unstemmed | Function of the ARGONAUTE protein ZWILLE in Arabidopsis shoot meristem stem cell maintenance vorgelegt von Sonia Boscá San Jose |
| title_short | Function of the ARGONAUTE protein ZWILLE in Arabidopsis shoot meristem stem cell maintenance |
| title_sort | function of the argonaute protein zwille in arabidopsis shoot meristem stem cell maintenance |
| topic | Embryonalentwicklung (DE-588)4070792-1 gnd Apikalmeristem (DE-588)4271341-9 gnd Proteine (DE-588)4076388-2 gnd Stammzelle (DE-588)4665329-6 gnd Ackerschmalwand (DE-588)4141299-0 gnd |
| topic_facet | Embryonalentwicklung Apikalmeristem Proteine Stammzelle Ackerschmalwand Hochschulschrift |
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