Basic cell culture protocols:
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | German |
| Veröffentlicht: |
New York [u.a.]
Humana Press, Springer
2013
|
| Ausgabe: | 4. ed. |
| Schriftenreihe: | Methods in molecular biology
946 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Klappentext |
| Beschreibung: | XIV, 550 S. Ill., graph. Darst. |
| ISBN: | 9781627031271 |
Internformat
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| 245 | 1 | 0 | |a Basic cell culture protocols |c ed. by Cheryl D. Helgason ... |
| 250 | |a 4. ed. | ||
| 264 | 1 | |a New York [u.a.] |b Humana Press, Springer |c 2013 | |
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| 490 | 1 | |a Methods in molecular biology |v 946 | |
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| 650 | 4 | |a Cell Culture Techniques |x methods |v Laboratory Manuals | |
| 650 | 4 | |a Cell culture |v Laboratory manuals | |
| 650 | 4 | |a Culture Techniques |x methods |v Laboratory Manuals | |
| 650 | 4 | |a Tissue culture |v Laboratory manuals | |
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Datensatz im Suchindex
| _version_ | 1804149547259133952 |
|---|---|
| adam_text | Contents
Preface
.......................................................... v
Contributors
...................................................... xi
1
Detection of Mycoplasma Contaminations
........................... 1
Cord C. Uphoffand Hans G. Drexler
2
Eradication of Mycoplasma Contaminations
.......................... 15
Cord C. Uphoffand Hans G. Drexler
3
STR
DNA
Typing of Human Cell lines: Detection of Intra-
and
Interspecies
Cross-Contamination
.............................. 27
Wilhelm G.
Dirb and Hans
G. Drexler
4
Classical and Molecular Cytogenetic Analysis
.......................... 39
Roderick A.F. MacLeod and Hans G. Drexler
5
Fluorescent In Situ Hybridization of
DNA
Probes in the
Interphase
and Metaphase Stages of the Cell Cycle
.............................. 61
Linda A. Cannizzaro
6
The Development of
Τ
Lymphocytes in Fetal
Thymus
Organ Culture
....... 85
Takeshi NitUt,
Izumi
Ohigashi, and Tousuke Takahama
7
Generation, Isolation, and Engraftment of In Vitro-Derived
Human
Τ
Cell Progenitors
....................................... 103
Geneve Awong and Juan Carlos
Zúñiga-Pflücker
8
In Vitro Generation of Human
Τ
Regulatory Cells: Generation, Culture,
and Analysis of FOXP3 -Transduced
Τ
Cells
........................... 115
Alicia
N.
McMurchy and Megan K. Levings
9
Simultaneous Cloning and Selection of
Hybridomas
and Transfected Cell
Lines in
Semisolid
Media
......................................... 133
Bert Wognum and Tracy Lee
10
Isolation and Characterization of Mouse Side Population Cells
............ 151
Aysegul V. Ergen, Mirajeong, Kuanyin K. Lin, Grant
A. Challen,
and Margaret A. Goodell
11
Stem Cell Identification by DyeCyde Violet Side Population Analysis
....... 163
William G. Telford
12
Isolation and Characterization of Cancer Stem Cells In Vitro
............. 181
Craig Gedye and Laurie
Ailles
13
Ex Vivo Differentiation of Cord Blood Stem Cells into Megakaryocytes
and Platelets
.................................................. 205
Nicolas Pineault,
Amélie
Robert,
Valérie Cortin,
and
Lucie
Boyer
Vil
viii Contents
14 Generation
and Characterization of
Murine
Alternatively
Activated
Macrophages
.......................................... 225
Shelley
В.
Weisser, Keith W. McLarren, Etsushi Kuroda, and Laura M. Sly
15
Human
Long-Term
Culture Initiating Cell Assay
...................... 241
Min
Liu, Cindy L. Miller, and Connie J. Eaves
16
Long-Term
Culture-Initiating Cell Assay for Mouse Cells
................ 257
Stefan Woehrer, Cindy L. Miller, and Connie J. Eaves
17
Colony Forming Cell Assays for Human Hematopoietic Progenitor Cells
.... 267
Bert Wognum,
Ning
Tuan, Becky Lai, and Cindy L. Miller
18
Studying Leukocyte Recruitment Under Flow Conditions
................ 285
Sean A. Parsons,
Christophe
Jurzinsky, Susan L. Cuvelier,
and
Ramala D.
Patel
19
Generation and Establishment of
Murine
Adherent Cell Lines
............. 301
Rouzanna lstvanffy and Robert A.J.
Oostendorp
20
Isolation, Enumeration, and Expansion of Human Mesenchymal
Stem Cells in Culture
........................................... 315
Ravenska Wagey and Brenton Short
21
Isolation and Culture of Mesenchymal Stem Cells from Mouse
Compact Bone
................................................ 335
Brenton Short and Ravenska Wagey
22
Generation of a Pool of Human Platelet
Lysáte
and Efficient
Use in Cell Culture
............................................. 349
Katharina Schallmoser
and
Dirk Strunk
23
In Vitro Methods to Culture Primary Human Breast Epithelial Cells
........ 363
Afihin Raoufand Yujia Sun
24
Human Prostate Epithelial Cell Cultures
............................. 383
Johng S. Rhim
25
Enzymatic Dissociation, Flow Cytometric Analysis, and Culture
of Normal Mouse Mammary Tissue
................................ 395
Michael Prater,
Mona
Shehata, Christine J. Watson, and John
Stingi
26
Isolation and Characterization of Human Hair Follicle Epithelial Cells
...... 411
Keita Inoue and
Kotaro Yoshimura
27
Cocultivation of Human Oral Keratinocytes and Human
Osteoblast-Iike Cells
........................................... 423
Ricarda
Glaum and
Margit Wiedmann-
Al-
Ahmad
28
Isolation and Culture of Skeletal Muscle Myofibers as a Means to
Analyze Satellite Cells
........................................... 431
Paul
Keire,
Andrew Shearer, GabiShefer, andZipora Yablonka-Reuveni
29
Hepatic Differentiation of Embryonic Stem Cells by
Murine
Fetal Liver
Mesenchymal Cells
............................................. 469
Takamichi Ishii, Kentaro Yasuchika, and Iwao Ikai
30
Methods to Culture, Differentiate, and Characterize Neural Stem Cells
from the Adult and Embryonic Mouse Central Nervous System
........... 479
Sharon A. Louis, Carmen K.H.
Mak,
and Brent A. Reynolds
Contents ix
31
Feeder-Independent Culture Systems for Human Pluripotent Stem Cells
..... 507
Jennifer Moody
32
Formation of Embryoid Bodies from Human Pluripotent Stem Cells
Using AggreWell™ Plates
........................................ 523
Jennifer Antonchuk
33
Techniques in Embryoid Body Formation from Human Pluripotent
Stem Cells
.................................................... 535
Nirupama K. Shevde and Amber A. Mael
Index
............................................................... 547
Methods in Molecular Biology
946
John M. Walker, Series Editor
Cheryl D.
Helgason
■
Cindy L. Miller Editors
Basic Celt Culture Protocols
Fourth Edition
At some point in their careers, virtually every scientist and technician, as well as many medical
professionals, regardless of their area of specialization have a need to utilize cell culture systems.
Updating and significantly expanding upon the previous editions, Basic Cell Culture Protocols,
ľoitrtlí
Edition provides the novice cell culturist with sufficient information to perform the
basic techniques, to ensure the health and
identit}·
of their cell lines, and to be able to isolate
and culture specialized
primar}·
cell types. The intent of this extensive volume is to generate a
valuable resource containing clear methodologies pertinent to current areas of investigation,
rather than attempting to educate cell culturists on specific cell types or organ systems. Written
in the highly successful Methods in Molecular Biology
,
chapters include introductions to their
respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible
laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and up-to-date, Basic Cell Culture Protocols, Fourth Edition compiles the es¬
sential techniques needed to approach this vital laboratory activity with full success.
|
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| language | German |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Basic cell culture protocols ed. by Cheryl D. Helgason ... 4. ed. New York [u.a.] Humana Press, Springer 2013 XIV, 550 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 946 Springer protocols Biologia molecular (métodos) larpcal Cultura de células larpcal Cultura de tecidos larpcal Técnicas e procedimentos de laboratório larpcal Cell Culture Techniques methods Laboratory Manuals Cell culture Laboratory manuals Culture Techniques methods Laboratory Manuals Tissue culture Laboratory manuals Gewebekultur (DE-588)4157245-2 gnd rswk-swf Zellkultur (DE-588)4067547-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Zellkultur (DE-588)4067547-6 s DE-604 Gewebekultur (DE-588)4157245-2 s b DE-604 Helgason, Cheryl D. edt Erscheint auch als Online-Ausgabe 978-1-62703-128-8 Methods in molecular biology 946 (DE-604)BV035362695 946 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025332912&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025332912&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Klappentext |
| spellingShingle | Basic cell culture protocols Methods in molecular biology Biologia molecular (métodos) larpcal Cultura de células larpcal Cultura de tecidos larpcal Técnicas e procedimentos de laboratório larpcal Cell Culture Techniques methods Laboratory Manuals Cell culture Laboratory manuals Culture Techniques methods Laboratory Manuals Tissue culture Laboratory manuals Gewebekultur (DE-588)4157245-2 gnd Zellkultur (DE-588)4067547-6 gnd |
| subject_GND | (DE-588)4157245-2 (DE-588)4067547-6 (DE-588)4143413-4 |
| title | Basic cell culture protocols |
| title_auth | Basic cell culture protocols |
| title_exact_search | Basic cell culture protocols |
| title_full | Basic cell culture protocols ed. by Cheryl D. Helgason ... |
| title_fullStr | Basic cell culture protocols ed. by Cheryl D. Helgason ... |
| title_full_unstemmed | Basic cell culture protocols ed. by Cheryl D. Helgason ... |
| title_short | Basic cell culture protocols |
| title_sort | basic cell culture protocols |
| topic | Biologia molecular (métodos) larpcal Cultura de células larpcal Cultura de tecidos larpcal Técnicas e procedimentos de laboratório larpcal Cell Culture Techniques methods Laboratory Manuals Cell culture Laboratory manuals Culture Techniques methods Laboratory Manuals Tissue culture Laboratory manuals Gewebekultur (DE-588)4157245-2 gnd Zellkultur (DE-588)4067547-6 gnd |
| topic_facet | Biologia molecular (métodos) Cultura de células Cultura de tecidos Técnicas e procedimentos de laboratório Cell Culture Techniques methods Laboratory Manuals Cell culture Laboratory manuals Culture Techniques methods Laboratory Manuals Tissue culture Laboratory manuals Gewebekultur Zellkultur Aufsatzsammlung |
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