Mesenchymal stem cell grafts promote oligodendroglial differentiation after spinal cord injury:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online-Zugang: | kostenfrei https://nbn-resolving.org/urn:nbn:de:bvb:355-epub-259949 Inhaltsverzeichnis |
| Beschreibung: | 72 Bl. Ill., graph. Darst. |
Internformat
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Datensatz im Suchindex
| _version_ | 1804149518011203584 |
|---|---|
| adam_text | IMAGE 1
CONTENTS
CONTENTS
SUMMARY 3
1. INTRODUCTION 5
1.1. SPINAL CORD INJURY....- - - - S
1.1.1. EPIDEMIOLOGY 5
1.1.2. CLASSIFICATION OF SPINAL CORD INJURY 6
1.1.3. SIGNS AND SYMPTOMS O F SPINAL CORD INJURY 6
1.1.4. CURRENT CONCEPTS O F SPINAL CORD INJURY TREATMENT 7
1.1.5. PATHOMECHANISMS O F SPINAL CORD INJURY 8
1.1.5.1. GROWTH INHIBITORY COMPONENTS AND FACTORS AFFECTING INTRINSIC
REGENERATIVE CAPACITY 8
1.1.5.2. DEMYELINATION 10
1.1.5.3. REMYELINATION 11
1.2. REGENERATIVE STRATEGIES AFTER SPINAL CORD INJURY 13
1.2.1. AXONAL REPAIR 13
1.2.2. STRATEGIES FOR REMYELINATION 13
1.2.2.1. TRANSPLANTATION OF MYELIN-FORMING CELLS 14
1.2.2.2. STIMULATION OF ENDOGENOUS PROGENITOR CELLS FOR REMYELINATION 15
2. AIM O F THE THESIS 16
3. MATERIAL & METHODS 17
3.1.CHEMICAIS & MATERIALS 17
3.1.1. CELL CULTURE 17
3.1.2. IMMUNODETECTION 17
3.1.3. OTHER CHEMICALS + KITS 18
3.1.4. ANTIBODIES 18
3.1.5. BUFFER AND SOLUTIONS 19
3.1.6. CONSUMABLES 20
3.1.7. SOFTWARE 20
3.1.8. EQUIPMENT AND INSTRUMENTS 20
3.2. METHODS - 21
3.2.1. ANIMAL SUBJECTS 21
3.2.2. PREPARATION AND CELL CULTURE 21
3.2.2.1. PREPARATION OF NEURAL PROGENITOR CELLS (NPC) 21
3.2.2.2. PREPARATION OF FIBROBLASTS (FF) 23
3.2.2.3. PREPARATION O F MESENCHYMAL STEM CELLS (MSC) 23
3.2.2.4. PREPARATION AND USE O F CONDITIONED MEDIA 24
3.2.2.5. IN VITRO DIFFERENTIATION ASSAYS 24
3.2.3. IMMUNOCYTOCHEMISTRY 25
3.2.4. PREPARATION OF RESPECTIVE CELL TYPES FOR TRANSPLANTATION 26
3.2.5. SURGICAL PROCEDURES 26
3.2.5.1. CERVICAL DORSAL COLUMN TRANSECTION 26
3.2.5.2. CELL TRANSPLANTATION INTO THE INJURED SPINAL CORD 27
3.2.6. BRDU-INJECTION AND EXPERIMENTAL DESIGN OF THE IN VIVO STUDIES 28
3.2.7. HISTOLOGY 29
3.2.8. IMMUNHISTOCHEMISTRY 30
3.2.8.1. BRIGHTFIELD ANALYSIS 30
3.2.8.2. FLUORESCENCE ANALYSIS 31
3.2.9. MICROSCOPICAL ANALYSIS 32
3.2.10. STATISTICAL ANALYSIS 33
1
HTTP://D-NB.INFO/1028536313
IMAGE 2
CONTENTS
4. RESULTS 34
4.1. IN VITRO 34
4.1.1. MSC AND FF DERIVED SOLUBLE FACTORS INDUCE OLIGODENDROGLIAL
DIFFERENTIATION IN NPC IN VITRO 34
4.2. IN VIVO 37
4.2.1. MSC AND FIBROBLAST GRAFTS REPLACE THE CYSTIC LESION DEFECT IN THE
INJURED SPINAL CORD 37
4.2.2. MSC TRANSPLANTATION DOES NOT ALTER THE PROLIFERATION AND SURVIVAL
O F NEWBORN CELLS AFTER SPINAL CORD INJURY 38
4.2.3. MSC ENHANCE OLIGODENDROGLIAL DIFFERENTIATION IN ENDOGENOUS NPC
ALREADY WITHIN 3 DAYS AFTER SCI 40
4.2.4. MSC GRAFTS SHIFT THE DIFFERENTIATION O F ENDOGENOUS PROGENITOR
CELLS TOWARDS OLIGODENDROGLIA FOUR WEEKS AFTER SCI 42
5. DISCUSSION 4 3
6. CONCLUSION 49
7. ABBREVIATIONS 50
8. REFERENCES 54
9. ATTACHMENTS 69
9.1. CURRICULUM VITAE 69
9.2. ACKNOWLEDGMENTS 71
2
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| spelling | Bründl, Johannes 1984- Verfasser (DE-588)1026531845 aut Mesenchymal stem cell grafts promote oligodendroglial differentiation after spinal cord injury vorgelegt von Johannes Bründl 2012 72 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Regensburg, Univ., Diss., 2012 Mesenchym (DE-588)4137719-9 gnd rswk-swf Zelldifferenzierung (DE-588)4067536-1 gnd rswk-swf Stammzelle (DE-588)4665329-6 gnd rswk-swf Rückenmarksverletzung (DE-588)4178632-4 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Rückenmarksverletzung (DE-588)4178632-4 s Stammzelle (DE-588)4665329-6 s Mesenchym (DE-588)4137719-9 s Zelldifferenzierung (DE-588)4067536-1 s b DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:355-epub-259949 http://epub.uni-regensburg.de/25994/ Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:355-epub-259949 Resolving-System DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025302923&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Bründl, Johannes 1984- Mesenchymal stem cell grafts promote oligodendroglial differentiation after spinal cord injury Mesenchym (DE-588)4137719-9 gnd Zelldifferenzierung (DE-588)4067536-1 gnd Stammzelle (DE-588)4665329-6 gnd Rückenmarksverletzung (DE-588)4178632-4 gnd |
| subject_GND | (DE-588)4137719-9 (DE-588)4067536-1 (DE-588)4665329-6 (DE-588)4178632-4 (DE-588)4113937-9 |
| title | Mesenchymal stem cell grafts promote oligodendroglial differentiation after spinal cord injury |
| title_auth | Mesenchymal stem cell grafts promote oligodendroglial differentiation after spinal cord injury |
| title_exact_search | Mesenchymal stem cell grafts promote oligodendroglial differentiation after spinal cord injury |
| title_full | Mesenchymal stem cell grafts promote oligodendroglial differentiation after spinal cord injury vorgelegt von Johannes Bründl |
| title_fullStr | Mesenchymal stem cell grafts promote oligodendroglial differentiation after spinal cord injury vorgelegt von Johannes Bründl |
| title_full_unstemmed | Mesenchymal stem cell grafts promote oligodendroglial differentiation after spinal cord injury vorgelegt von Johannes Bründl |
| title_short | Mesenchymal stem cell grafts promote oligodendroglial differentiation after spinal cord injury |
| title_sort | mesenchymal stem cell grafts promote oligodendroglial differentiation after spinal cord injury |
| topic | Mesenchym (DE-588)4137719-9 gnd Zelldifferenzierung (DE-588)4067536-1 gnd Stammzelle (DE-588)4665329-6 gnd Rückenmarksverletzung (DE-588)4178632-4 gnd |
| topic_facet | Mesenchym Zelldifferenzierung Stammzelle Rückenmarksverletzung Hochschulschrift |
| url | http://epub.uni-regensburg.de/25994/ https://nbn-resolving.org/urn:nbn:de:bvb:355-epub-259949 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025302923&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT brundljohannes mesenchymalstemcellgraftspromoteoligodendroglialdifferentiationafterspinalcordinjury |