Cell senescence: methods and protocols
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Humana Press
2013
|
| Schriftenreihe: | Methods in molecular biology
965 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XII, 538 S. Ill., graph. Darst. |
| ISBN: | hardback 9781627032384 |
Internformat
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| 245 | 1 | 0 | |a Cell senescence |b methods and protocols |c ed. by Lorenzo Galluzzi ... |
| 264 | 1 | |a New York [u.a.] |b Humana Press |c 2013 | |
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Datensatz im Suchindex
| _version_ | 1804149499713552384 |
|---|---|
| adam_text | Contents
Preface
.................................................................
v
Contributors
............................................................. ix
1
Cell Senescence as Both a Dynamic and a Static Phenotype
.................. 1
Andrew R.J. Young,
Musako
Narita, and Masashi Narita
2
Senescence Regulation by mTOR
..................................... 15
Vjekoslav
Dulie
3
Senescence Regulation by the p53 Protein Family
......................... 37
Yingjuan Qian andXinbin Chen
4
Markers of Cellular Senescence
....................................... 63
Amando Carnero
5
Biomarkers
of Cell Senescence Assessed by Imaging Cytometry
.............. 83
Hong Zhao and
Zbigniew Darzynkiewicz
6
Cytofluorometric Assessment of Cell Cycle Progression
.................... 93
Ilio Vitale,
Mohamed
Jemaà,
Lorenzo
Galluzzi,
Didier
Metívier, Maria
Castedo, and Guido Kroemer
7
Quantification
of Cell Cycle-Arresting Proteins
........................... 121
Oliver Kepp,
Isabelle
Martins, Laurie
Menger, Michael Michaud,
Sandy Adjemian, Abdul Qader Sukkurwala, Lorenzo Galluzzi,
and
Guido
Kroemer
8
Colorimetrie
Detection of Senescence-Associated
β
Galactosidase
............. 143
Kop Itahana,
Toko Itahana, and Goberdhan P. Dimri
9
Chemiluminescent Detection of Senescence-Associated
β
Galactosidase
........ 157
Vinícius
Bassaneze, Ayumi
Aurea
Miyakawa, and
José Eduardo
Krieger
10
Detection of the Senescence-Associated Secretory Phenotype (SASP)
.......... 165
Francis Kodier
11
Unbiased Characterization of the Senescence-Associated
Secretome
Using SILAC-Based Quantitative Proteomics
............................ 175
Juan Carlos
Acosta,
Ambrosius
P.
Snijders,
and
Jesús Gil
12
Detection of Senescence-Associated Heterochromatin Foci (SAHF)
........... 185
Katherine M. Aird
and Rugang Zhang
13
Monitoring
DNA
Damage During Cell Senescence
....................... 197
Glyn Nelson and Thomas
von Zglinicki
14
Assessment and Quantification of Telomerase Enzyme Activity
............... 215
Michelle F.
Marita,
Laura A. Richards, and Karen L· MacKenzie
15
Methods for the Assessment of Telomere Status
.......................... 233
AsakoJ. Nakamura
VII
viii Contents
16
Detection of Nuclear Envelope Alterations in Senescence
................... 243
Clea
Barcena,
Fernando G.
Osório,
and
José
Maria
Pérez Freije
17
Measuring Reactive Oxygen Species in Senescent Cells
..................... 253
João
F.
Passos,
Satomi Miwa,
and Thomas
von Zglinicki
18
Quantification of Protein Carbonylation
................................ 265
Nancy B.
Wehr
and Rodney L.
Levine
19
Assays for the Measurement of
Lipid
Peroxidation........................
283
Ana Cipak Gasparovic,
Morana
Jaganjac, Branka Mihaljevic,
Suzana
Borovic
Sunjic, and Neven Zarkovic
20
Raman Spectroscopy for the Detection of AGEs/ALEs
.................... 297
J. Renwick Beattie, John J. McGarvey, and Alan W. Stitt
21
Monitoring Oncogenic B-RAF-Induced Senescence in Melanocytes
........... 313
Sieu L.
Tran
and Helen
Rizos
22
Methods to Investigate the Role of SIRT1 in Endothelial Senescence
.......... 327
Bo
Bai
and
Tu Wang
23
Monitoring Nutrient Signaling Through the Longevity Protein p66SHC1
........ 341
Sofia Chiatamone Ranieri and Giovambattista
Pani
24
Profiling the Metabolic Signature of Senescence
.......................... 355
Florian
M.
Geier, Silke Fuchs, Gabriel Valbuena,
Armand M. Leroi, and Jacob
G. Bundy
25
Genome-Wide RNAi Screening to Identify Regulators of
Oncogene-
Induced Cellular Senescence
................................ 373
Narendra
Wajapeyee, Sara K. Deibler, and Michael R. Green
26
An Integrated Approach for Monitoring Cell Senescence
................... 383
Tatiana
V. Postelová, Zhanna
V. Chitikova, and
Valéry
A. Pospelov
27
Robust Multiparametric Assessment of Cellular Senescence
.................. 409
Clara Correia-Melo, Diana
Jurk,
and
João
F.
Passos
28
Assessing Chronological Aging in Bacteria
.............................. 421
Stavros Gonidakis and
Valter D.
Longo
29
Assessing
Organismal
Aging in the Filamentous Fungus Podospora anserina
..... 439
Heinz D. Osiewacz, Andrea
Hamann,
and Sandra Zintel
30
Assessing Chronological Aging in Saccharomyces cerevinae
.................. 463
Jia
Ни,
Min
Wei, Mario
G.
Mirisala, and
Valter D.
Longo
31
Assessing Aging and Senescent Decline in Caenorhabditis
elegans:
Cohort Survival Analysis
............................................ 473
Eirini
Lionati
and Nektarios Tavernarakis
32
High-Throughput and Longitudinal Analysis of Aging
and Senescent Decline in Caenorhabditis
elegans
.......................... 485
Eirini Lionaki and Nektarios Tavernarakis
33
Assessing Senescence in
Drosophila
Using Video Tracking
.................. 501
Reza
Ardekani, Simon
Tavaré,
and John Tower
34
Assessing Vascular Senescence in Zebrafish
.............................. 517
Sandra Donnini, Antonio
Giochetti,
and Marina
Ziehe
Index
.............................................................. 533
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| title | Cell senescence methods and protocols |
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