De novo patterning in Arabidopsis thaliana: studies on patterning complex composition and transcriptional regulation of GLABRA3
Zsfassung in dt. und engl. Sprache
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Zusammenfassung: | Zsfassung in dt. und engl. Sprache |
| Beschreibung: | XIII, 98, IX S. Ill., graph. Darst. |
Internformat
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Datensatz im Suchindex
| _version_ | 1804149454519926784 |
|---|---|
| adam_text | Titel: De novo patterning in Arabidopsis thaliana
Autor: Friede, Alexandra
Jahr: 2012
Contents
Abstract III
Zusammenfassung V
List of Figures VII
List of Tables IX
List of Abbreviations XIII
1 Introduction 1
1.1 The Model Organism Arabidopsis thaliana.................... 1
1.2 Patterning in Arabidopsis thaliana......................... 2
1.3 De novo Patterning in Arabidopsis thaliana.................... 2
1.4 Mathematical Models Describe de novo Patterning................ 3
1.5 Mathematical Models Became Biological Models................. 4
1.5.1 Activators ................................... 6
1.5.2 Inhibitors.................................... 6
1.5.3 Biological Model................................ 7
1.6 The bHLH Domain Protein Family......................... 8
1.7 The bHLH Transcription Factor GL3........................ 9
1.8 The Regulatory Regions for GL3 Transcription.................. 9
1.9 Aim of Research.................................... 10
2 Results 13
2.1 Studies on Patterning Protein Complexes..................... 13
2.1.1 Patterning Protein Expression in Tobacco................. 13
2.1.2 Patterning Protein Expression in Arabidopsis thaliana......... 19
2.1.3 Complex Composition of Patterning Proteins Expressed in HEK Cells 23
2.2 Studies on the Transcriptional Regulation of GL3................ 28
2.2.1 Detailed Analysis of the GL3 Promoter Region.............. 29
2.2.2 Studies on the bHLH Domain of GL3 ................... 45
3 Discussion 57
3.1 Studies on Patterning Protein Complexes..................... 57
3.2 Studies on the Transcriptional Regulation of GL3................ 58
3.2.1 Detailed Analysis of the GL3 Promoter Region.............. 59
3.2.2 Studies on the bHLH Domain of GL3 ................... 61
3.3 Perspectives...................................... 64
4 Materials and Methods 67
4.1 General Materials................................... 67
4.2 Buffers and Solutions................................. 68
4.3 Oligonucleotides.................................... 70
4.4 Vectors and Plasmids................................. 75
4.5 Organisms....................................... 83
4.5.1 Bacterial Strains............................... 83
4.5.2 Yeast Strains.................................. 83
4.5.3 Cell Culture.................................. 83
4.5.4 Plant Mutants................................. 84
4.5.5 Transgenic Lines............................... 85
4.6 Yeast Methods..................................... 86
4.6.1 Lithium Acetate Transformation...................... 86
4.6.2 Yeast One-Hybrid Experiments....................... 87
4.6.3 Screening.................................... 87
4.7 Plant Methods..................................... 88
4.7.1 Genotyping of Plants............................. 88
4.7.2 Trichome Counting.............................. 88
4.7.3 Tobacco Infiltration.............................. 89
4.7.4 Arabidopsis thaliana Cell Suspension Culture Transformation .... 89
4.7.5 Arabidopsis thaliana Stable Plant Transformation ........... 89
4.8 Biochemical Methods................................. 89
4.8.1 Protein Extraction from Tobacco...................... 89
4.8.2 Protein Extraction from Arabidopsis thaliana .............. 90
4.8.3 Protein Extraction from HEK Cells..................... 90
4.8.4 TCA Protein Precipitation.......................... 90
4.8.5 Western Blot.................................. 91
4.8.6 Blue Native .................................. 91
4.8.7 Gel Filtration ................................. 91
4.8.8 Protein A/ Renilla Luciferase Co-Precipitation Assay.......... 92
4.8.9 GUS Quantification Assay of Arabidopsis thaliana Cell Suspension
Culture..................................... 92
Appendices 95
A Gap Repair Results 97
Bibliography i
Danksagung vii
Erklarung ix
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| spelling | Friede, Alexandra Verfasser (DE-588)1024259218 aut De novo patterning in Arabidopsis thaliana studies on patterning complex composition and transcriptional regulation of GLABRA3 vorgelegt von Alexandra Friede 2012 XIII, 98, IX S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Köln, Univ., Diss., 2012 Zsfassung in dt. und engl. Sprache (DE-588)4113937-9 Hochschulschrift gnd-content HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025256966&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Friede, Alexandra De novo patterning in Arabidopsis thaliana studies on patterning complex composition and transcriptional regulation of GLABRA3 |
| subject_GND | (DE-588)4113937-9 |
| title | De novo patterning in Arabidopsis thaliana studies on patterning complex composition and transcriptional regulation of GLABRA3 |
| title_auth | De novo patterning in Arabidopsis thaliana studies on patterning complex composition and transcriptional regulation of GLABRA3 |
| title_exact_search | De novo patterning in Arabidopsis thaliana studies on patterning complex composition and transcriptional regulation of GLABRA3 |
| title_full | De novo patterning in Arabidopsis thaliana studies on patterning complex composition and transcriptional regulation of GLABRA3 vorgelegt von Alexandra Friede |
| title_fullStr | De novo patterning in Arabidopsis thaliana studies on patterning complex composition and transcriptional regulation of GLABRA3 vorgelegt von Alexandra Friede |
| title_full_unstemmed | De novo patterning in Arabidopsis thaliana studies on patterning complex composition and transcriptional regulation of GLABRA3 vorgelegt von Alexandra Friede |
| title_short | De novo patterning in Arabidopsis thaliana |
| title_sort | de novo patterning in arabidopsis thaliana studies on patterning complex composition and transcriptional regulation of glabra3 |
| title_sub | studies on patterning complex composition and transcriptional regulation of GLABRA3 |
| topic_facet | Hochschulschrift |
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