Investment Guide China:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | German |
| Veröffentlicht: |
Aachen
Shaker
2012
|
| Schriftenreihe: | Berichte aus der Rechtswissenschaft
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | VIII, 191 S. |
| ISBN: | 9783844011869 |
Internformat
MARC
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Datensatz im Suchindex
| _version_ | 1804149451631099904 |
|---|---|
| adam_text | IMAGE 1
CONTENTS
1 MOTIVATION 1
1 INTRODUCTION 3
2 CELL ADHESION AND MECHANICS 5
2.1 CELL STRUCTURE 5
2.2 CELL ADHESION 7
2.3 MODEL SYSTEMS 11
3 LIPOSOME PREPARATION AND MEMBRANE PROTEIN RECONSTITUTION 17 3.1 SMALL
AND LARGE UNILAMELLAR VESICLES 17
3.2 GIANT UNILAMELLAR VESICLES 18
3.3 LIPOSOMES WITH INCORPORATED MEMBRANE PROTEINS 22
3.4 PREPARATION OF GIANT VESICLES CONTAINING PROTEIN 29
4 METHODS 3 1
4.1 PROTEINS 31
4.2 BIOCHEMICAL ANALYSIS OF PROTEINS 34
4.3 ANALYSIS OF SMALL VESICLES 39
4.4 ANALYSIS OF GIANT VESICLES - MICROSCOPY 43
II EXPERIMENTAL DESIGN AND MEASUREMENT TECHNIQUES 5 1
5 THE MINIMAL SYSTEM 5 3
6 PREPARATION O F SMALL AND GIANT VESICLES 55
6.1 SMALL UNILAMELLAR VESICLES 55
6.2 GIANT UNILAMELLAR VESICLES 57
6.3 PROTEIN INCORPORATION IN GUVS 61
7 CHARACTERIZATION O F VESICLES 65
7.1 SIZE MEASUREMENT WITH DYNAMIC LIGHT SCATTERING 65
7.2 DENSITY GRADIENT CENTRIFUGATION OF LIPOSOMES AND PROTEOLIPOSOMES .
66
VII
HTTP://D-NB.INFO/1029569398
IMAGE 2
CONTENTS
7.3 LIGHT MICROSCOPY TO STUDY GIANT UNILAMELLAR VESICLES 66
8 BIOCHEMISTRY 75
8.1 PROTEIN AND PLATELET MEMBRANE PURIFICATION 75
8.2 MEASURING THE CONCENTRATION OF PURIFIED INTEGRIN 77
8.3 SDS-POLYACRYLAMIDE GEL ELECTROPHORESIS 77
8.4 SPECIFIC DETECTION OF INTEGRIN 78
8.5 CO-IMMUNOPRECIPITATION OF INTEGRIN 80
III RESULTS AND DISCUSSION 83
9 CHARACTERIZATION O F PURIFIED PLATELET INTEGRIN 85
9.1 IDENTIFICATION OF PROTEINS PURIFIED FROM PLATELET MEMBRANES 85 9.2
PURITY AND YIELD OF PURIFIED INTEGRIN 89
9.3 MOLECULAR STATE OF PURIFIED INTEGRIN 91
9.4 FUNCTIONALITY OF PURIFIED INTEGRIN 97
10 RESULTS ON SMALL AND GIANT LIPOSOMES 101
10.1 SIZE DISTRIBUTION OF SUVS 101
10.2 ANALYSIS OF INTEGRIN RECONSTITUTION IN SMALL VESICLES 104
10.3 PREPARATION OF GUVS USING DIFFERENT METHODS 107
10.4 ENCLOSURE OF ACTIN IN GUVS I L L
10.5 PREPARATION OF INTEGRIN-GUVS FROM PROTEOLIPOSOMES 112
10.6 PREPARATION OF GUVS FROM PLATELET MEMBRANES 119
11 ADHESIVE PROPERTIES O F THE MODEL SYSTEM 121
11.1 GUVS FROM PLATELET MEMBRANES ON NANOSTRUCTURED SURFACES 121
12 CONCLUSIVE REMARKS AND FURTHER EXPERIMENTS 127
ABBREVIATIONS 131
LIST O F FIGURES 133
LIST O F TABLES 137
BIBLIOGRAPHY 139
A PHASE CONTRAST, DIFFERENTIAL INTERFERENCE CONTRAST AND FLUORESCENCE
MI CROSCOPY 163
B MOVIES 167
VIII
IMAGE 3
CONTENTS
C MATLAB ROUTINES FOR EVALUATION O F RICM IMAGES 169
D CHEMICALS AND MATERIALS 187
D.L GENERAL EQUIPMENT 187
D.2 GIANT VESICLES 187
D.3 BIOCHEMICAL METHODS 188
D.4 PROTEIN PURIFICATION 189
D.5 AGAROSE-LIPID-HYBRID FILMS 190
E LIPID MIXTURES 191
F BUFFERS 193
F . L INTEGRIN 193
F.2 PLATELET MEMBRANES 194
F.3 ACTIN 195
F.4 INNER AND OUTER BUFFER FOR ADHESION EXPERIMENTS WITH PLATELET-GUVS
195 F.5 SDS-PAGE AND WESTERN BLOT 196
G PROTOCOLS FOR PROTEIN PURIFICATION AND THEIR CHARACTERIZATION 197 G.L
PURIFICATION OF INTEGRIN FROM PLATELET CONCENTRATE 197
G.2 ELISA FOR INTEGRIN BINDING ACTIVITY 199
G.3 LABELING OF INTEGRIN WITH TAMRA 200
G.4 PURIFICATION OF PLATELET MEMBRANES 200
ACKNOWLEDGMENT 203
ERKLARUNG 205
IX
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| dewey-tens | 340 - Law |
| discipline | Rechtswissenschaft Wirtschaftswissenschaften |
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| illustrated | Not Illustrated |
| indexdate | 2024-07-10T00:23:14Z |
| institution | BVB |
| isbn | 9783844011869 |
| language | German |
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| physical | VIII, 191 S. |
| publishDate | 2012 |
| publishDateSearch | 2012 |
| publishDateSort | 2012 |
| publisher | Shaker |
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| series2 | Berichte aus der Rechtswissenschaft |
| spelling | Lorenz, Michael Verfasser aut Investment Guide China Michael Lorenz Aachen Shaker 2012 VIII, 191 S. txt rdacontent n rdamedia nc rdacarrier Berichte aus der Rechtswissenschaft Auslandsinvestition (DE-588)4003772-1 gnd rswk-swf Steuerrecht (DE-588)4116614-0 gnd rswk-swf Deutschland (DE-588)4011882-4 gnd rswk-swf China (DE-588)4009937-4 gnd rswk-swf Deutschland (DE-588)4011882-4 g China (DE-588)4009937-4 g Auslandsinvestition (DE-588)4003772-1 s Steuerrecht (DE-588)4116614-0 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025255085&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Lorenz, Michael Investment Guide China Auslandsinvestition (DE-588)4003772-1 gnd Steuerrecht (DE-588)4116614-0 gnd |
| subject_GND | (DE-588)4003772-1 (DE-588)4116614-0 (DE-588)4011882-4 (DE-588)4009937-4 |
| title | Investment Guide China |
| title_auth | Investment Guide China |
| title_exact_search | Investment Guide China |
| title_full | Investment Guide China Michael Lorenz |
| title_fullStr | Investment Guide China Michael Lorenz |
| title_full_unstemmed | Investment Guide China Michael Lorenz |
| title_short | Investment Guide China |
| title_sort | investment guide china |
| topic | Auslandsinvestition (DE-588)4003772-1 gnd Steuerrecht (DE-588)4116614-0 gnd |
| topic_facet | Auslandsinvestition Steuerrecht Deutschland China |
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