Functional polymer brushes on diamond electrodes for amperometric biosensor applications:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online-Zugang: | Volltext https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20120723-1096938-1-1 Inhaltsverzeichnis |
| Beschreibung: | 170 S. Ill., graph. Darst. |
Internformat
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| 245 | 1 | 0 | |a Functional polymer brushes on diamond electrodes for amperometric biosensor applications |c Aurelia Naima Hutter |
| 264 | 1 | |c 2012 | |
| 300 | |a 170 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
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Datensatz im Suchindex
| _version_ | 1804149447056162816 |
|---|---|
| adam_text | IMAGE 1
TABLE OF CONTENTS
1. INTRODUCTION 1
2. THEORETICAL BACKGROUND 3
2 . 1 PRINCIPLES OF A BIOSENSOR 3
2.1.1 GENERAL FUNCTION O F A BIOSENSOR 4
2.1.2 TYPES O F BIOSENSORS 5
2.1.2.1 ELECTROCHEMICAL BIOSENSORS 5
2.1.2.2 FIELD-EFFECT TRANSISTORS 9
2.1.2.3 SURFACE PLASMON RESONANCE 10
2.1.2.4 QUARTZ CRYSTAL MICROBALANCE 12
2.1.2.5 OTHER TECHNIQUES 14
2.1.3 DETAILED INSIGHT IN GLUCOSE BIOSENSORS 15
2.1.3.1 GENERAL DETECTION STRATEGIES FOR GLUCOSE 16
2.1.3.2 IMPLANTABLE SENSORS 19
2 . 2 (BIO)CHEMICAL FUNCTIONALIZATION OF ELECTRODE MATERIALS 2 1
2.2.1 BIOFUNCTIONALIZED SELF-ASSEMBLED MONOLAYERS 21
2.2.1.1 THIOLS ON GOLD 21
2.2.1.2 SILANE FUNCTIONALIZATION 22
2.2.1.3 DIAZONIUM SALTS 23
2.2.1.4 OTHER MONOLAYER APPROACHES 24
2.2.2 COATINGS 25
2.2.3 POLYMER BRUSH - BIOMOLECULE CONJUGATES 26
2.2.3.1 GENERAL REMARKS ON POLYMER BRUSHES 27
2.2.3.2 POLYELECTROLYTES AS MATRIX FOR BIOMOLECULES 29
2.2.3.3 COVALENTLY BONDED BIOMOLECULES 3 1
2.2.3.4 BIOLOGICAL ASPECTS OF POLY(2-OXAZOLINE)-BASED MOLECULAR BRUSHES
33
2.2.3.5 PREPARATION OF MICRO-AND NANOSTRUCTURED POLYMER BRUSHES 34
2 . 3 PROPERTIES AND APPLICATION OF DIAMOND ELECTRODES 4 0
HTTP://D-NB.INFO/103209284X
IMAGE 2
2.3.1 PROCESSING O F DIAMOND 40
2.3.2 PHYSICOCHEMICAL PROPERTIES O F DIAMOND 41
2.3.3 SURFACE MODIFICATION O F DIAMOND 42
2.3.3.1 SURFACE TERMINATION OF SYNTHETIC DIAMOND FILMS., 42
2.3.3.2 ORGANIC MONOLAYERS ON DIAMOND - STATE OF THE ART 43
2.3.3.3 POLYMER BRUSHES ON DIAMOND - STATE OF THE ART 44
2.3.4 BIOLOGICAL ASPECTS O F DIAMOND 45
3. PURPOSE AND OBJECTIVES 47
4. RESULTS AND DISCUSSION 49
4 . 1 MICRO- AND NANOSTRUCTURED POLYMER BRUSHES ON DIAMOND AND SIO X BY
S I P G P 4 9
4.1.1 REACTIVITY CONTRAST BETWEEN H- AND OH-TERMINATED DIAMOND 49
4.1.1.1 MICROSTRUCTURED POLYMER BRUSHES ON DIAMOND BY COMMON
PHOTOLITHOGRAPHY
50
4.1.1.2 POLY(2-OXAZOLINE) BOTTLE-BRUSH BRUSHES ON DIAMOND 52
4.1.2 ELECTRON-BEAM LITHOGRAPHY (EBL) ON NANOCRYSTALLINE DIAMOND 5 5
4.1.2.1 INVESTIGATION O F DIFFERENT TEMPLATE DESIGNS FOR THE EBL ON
DIAMOND 55
4.1.2.2 EBL WITH AN ELECTRON MICROSCOPE WITHOUT A LITHOGRAPHY TOOL 6 1
4.1.3 ELECTRIC FIELD-INDUCED LITHOGRAPHY ON SIO X 62
4.1.4 THERMAL STRUCTURING O F POLY(L-ETHOXYETHYL METHACRYLATE) BRUSHES
65
4 . 2 BIOFUNCTIONALIZED POLYMER BRUSHES 6 7
4.2.1 BACKGROUND ON PROTEINS AND ENZYMES 67
4.2.2 COUPLING STRATEGIES F O R BIOMOLECULES 70
4.2.2.1 PREPARATION O F BIOFUNCTIONALIZED POLY(2-OXAZOLINE)E
BOTTLE-BRUSH BRUSHES.... 70
4.2.2.2 BIOMOLECULE COUPLING TO POLY(METHACRYLIC ACID) BRUSHES 75
4.2.3 ACTIVITY AND STABILITY O F POLYMER BRUSH PROTEIN CONJUGATES 79
4.2.3.1 DETECTION OF THE GREEN FLUORESCENT PROTEIN IN FLUORESCENCE
MICROSCOPY 79
4.2.3.2 COLORIMETRIC ESSAYS FOR THE DETERMINATION OF ENZYMATIC REACTION
KINETICS 84
4 . 3 POLYMER BRUSHES AS POLYMERIC MEDIATORS 9 2
4.3.1 THEORETICAL INSIGHT INTO VOLTAMMETRIC AND AMPEROMETRIC TECHNIQUES
92
4.3.2 CARBAZOLE AS HOLE CONDUCTING MOIETY IN POLY(2-OXAZOLINE)
BOTTLE-BRUSH BRUSHES... 97
4.3.3 FERROCENE AS REDOX MEDIATOR 101
4.3.3.1 INCORPORATION OF FERROCENE USING VINYLFERROCENE AS CO-MONOMER IN
SIPGP... 102
IMAGE 3
4.3.3.2 POLYMER ANALOGUE COUPLING OF AMINOFERROCENE TO P(MA) BRUSHES 105
4.3.3.3 POLYMER ANALOGUE COUPLING OF AMINOMETHYLFERROCENE IN P(MA)
BRUSHES 107
4 . 4 ELECTROCHEMICAL ANALYSIS OF BIOFUNCTIONALIZED POLYMER BRUSHES ON
DIAMOND ELECTRODES .. 1 1 2
4.4.1 ELECTROCHEMICAL ENZYME DETECTION WITHOUT MEDIATOR 112
4.4.2 APPLICATION O F FREE MEDIATOR IN SOLUTION 113
4.4.3 ENZYMATIC REACTIONS IN POLYMERIC MEDIATORS 114
4.4.4 COMPARISON BETWEEN MONOLAYER- AND POLYMER BRUSH BOUND ENZYMES 123
4.4.4.1 COLORIMETRIC ASSAYS 123
4.4.4.2. FLUORESCENCE BLEACHING 126
4.4.4.3 AMPEROMETRIC DETECTION O F GLUCOSE 127
5. SUMMARY 129
6. ZUSAMMENFASSUNG 136
7. EXPERIMENTAL PART 144
7 . 1 INSTRUMENTS AND METHODS 1 4 4
7 . 2 SOLVENTS, CHEMICALS, SUBSTRATES 1 4 7
7 . 3 SURFACE FUNCTIONALIZATION PROCEDURES 1 4 8
7.3.1 TREATMENT O F SURFACES 148
7.3.2 FORMATION OFSAMS AND POLYMER BRUSHES 149
7.3.3 POLYMER ANALOGUE REACTIONS 150
7 . 4 SYNTHESIS OF ORGANIC MOLECULES 1 5 3
8. REFERENCES 158
|
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| discipline | Chemie / Pharmazie Physik Biologie Chemie |
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| spelling | Hutter, Aurelia Naima Verfasser aut Functional polymer brushes on diamond electrodes for amperometric biosensor applications Aurelia Naima Hutter 2012 170 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Techn. Univ., Diss., 2012 Funktionalisierung Chemie (DE-588)4279323-3 gnd rswk-swf Biosensor (DE-588)4193016-2 gnd rswk-swf Elektrode (DE-588)4014247-4 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Biosensor (DE-588)4193016-2 s Elektrode (DE-588)4014247-4 s Funktionalisierung Chemie (DE-588)4279323-3 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:91-diss-20120723-1096938-1-1 http://mediatum.ub.tum.de/node?id=1096938 Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20120723-1096938-1-1 Resolving-System DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025251876&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Hutter, Aurelia Naima Functional polymer brushes on diamond electrodes for amperometric biosensor applications Funktionalisierung Chemie (DE-588)4279323-3 gnd Biosensor (DE-588)4193016-2 gnd Elektrode (DE-588)4014247-4 gnd |
| subject_GND | (DE-588)4279323-3 (DE-588)4193016-2 (DE-588)4014247-4 (DE-588)4113937-9 |
| title | Functional polymer brushes on diamond electrodes for amperometric biosensor applications |
| title_auth | Functional polymer brushes on diamond electrodes for amperometric biosensor applications |
| title_exact_search | Functional polymer brushes on diamond electrodes for amperometric biosensor applications |
| title_full | Functional polymer brushes on diamond electrodes for amperometric biosensor applications Aurelia Naima Hutter |
| title_fullStr | Functional polymer brushes on diamond electrodes for amperometric biosensor applications Aurelia Naima Hutter |
| title_full_unstemmed | Functional polymer brushes on diamond electrodes for amperometric biosensor applications Aurelia Naima Hutter |
| title_short | Functional polymer brushes on diamond electrodes for amperometric biosensor applications |
| title_sort | functional polymer brushes on diamond electrodes for amperometric biosensor applications |
| topic | Funktionalisierung Chemie (DE-588)4279323-3 gnd Biosensor (DE-588)4193016-2 gnd Elektrode (DE-588)4014247-4 gnd |
| topic_facet | Funktionalisierung Chemie Biosensor Elektrode Hochschulschrift |
| url | http://mediatum.ub.tum.de/node?id=1096938 https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20120723-1096938-1-1 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025251876&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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