Establishment of a multiplex real-time PCR assay for the detection and differentiation of wild-type and glycoprotein E-deleted vaccine strains of bovine herpesvirus type 1:
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| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Gießen
DVG-Service
2012
|
| Edition: | 1. Aufl. |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Parallel als Online-Ausg. erschienen |
| Physical Description: | 81 S. graph. Darst. |
| ISBN: | 9783863451004 |
Staff View
MARC
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| 245 | 1 | 0 | |a Establishment of a multiplex real-time PCR assay for the detection and differentiation of wild-type and glycoprotein E-deleted vaccine strains of bovine herpesvirus type 1 |c von Kerstin Wernike |
| 246 | 1 | 0 | |a polymerase chain reaction |
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| 650 | 7 | |a Bovine herpesvirus 1 |2 cabt | |
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Record in the Search Index
| _version_ | 1804149441798602752 |
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| adam_text | IMAGE 1
TABLE OF CONTENTS
I. INTRODUCTION 1
II. LITERATURE REVIEW 3
1. POLYMERASE CHAIN REACTION 3
1.1. BASIC PRINCIPLE 3
1.2. REAL-TIME POLYMERASE CHAIN REACTION 3
1.3. MULTIPLEX POLYMERASE CHAIN REACTION 8
1.4. OPTIMIZATION AND VALIDATION 11
2. BOVINE HERPESVIRUS TYPE 1 13
2.1. TAXONOMY AND MOLECULAR CHARACTERISTICS 13
2.2. GLYCOPROTEIN E 16
2.3. PATHOGENESIS, CLINICAL DISEASE, IMMUNOLOGY 16
2.4. LATENCY AND REACTIVATION 18
2.5. DIAGNOSIS 19
2.6. VACCINATION AND CONTROL PROGRAMS 19
III. RESULTS 23
WERNIKE K., HOFFMANN B., KALTHOFF D., KONIG P. AND BEER M., 2011
DEVELOPMENT AND VALIDATION O F A TRIPLEX REAL-TIME P C R ASSAY FOR THE
RAPID
DETECTION AND DIFFERENTIATION O F WILD-TYPE AND GLYCOPROTEIN E-DELETED
VACCINE STRAINS O F BOVINE HERPESVIRUS TYPE 1
IV. DISCUSSION 48
1. MULTIPLEX POLYMERASE CHAIN REACTION 48
2. DETECTION O F BOVINE HERPESVIRUS TYPE 1 GENOMES 51
3. CONCLUDING REMARKS 55
V. SUMMARY 56
VI. ZUSAMMENFASSUNG 57
VII. REFERENCES 59
VIII. ABBREVIATIONS 80
IX. ACKNOWLEDGEMENTS 81
HTTP://D-NB.INFO/1025330587
|
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| bvnumber | BV040393843 |
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| dewey-full | 636.208969112 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
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| dewey-tens | 630 - Agriculture and related technologies |
| discipline | Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau |
| edition | 1. Aufl. |
| format | Thesis Book |
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| institution | BVB |
| isbn | 9783863451004 |
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| spelling | Wernike, Kerstin 1981- Verfasser (DE-588)1026054974 aut Establishment of a multiplex real-time PCR assay for the detection and differentiation of wild-type and glycoprotein E-deleted vaccine strains of bovine herpesvirus type 1 von Kerstin Wernike polymerase chain reaction 1. Aufl. Gießen DVG-Service 2012 81 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Parallel als Online-Ausg. erschienen Zugl.: München, Univ., Diss., 2012 polymerase chain reaction cabt real time PCR cabt Bovine herpesvirus 1 cabt vaccines cabt glycoproteins cabt (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025247124&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Wernike, Kerstin 1981- Establishment of a multiplex real-time PCR assay for the detection and differentiation of wild-type and glycoprotein E-deleted vaccine strains of bovine herpesvirus type 1 polymerase chain reaction cabt real time PCR cabt Bovine herpesvirus 1 cabt vaccines cabt glycoproteins cabt |
| subject_GND | (DE-588)4113937-9 |
| title | Establishment of a multiplex real-time PCR assay for the detection and differentiation of wild-type and glycoprotein E-deleted vaccine strains of bovine herpesvirus type 1 |
| title_alt | polymerase chain reaction |
| title_auth | Establishment of a multiplex real-time PCR assay for the detection and differentiation of wild-type and glycoprotein E-deleted vaccine strains of bovine herpesvirus type 1 |
| title_exact_search | Establishment of a multiplex real-time PCR assay for the detection and differentiation of wild-type and glycoprotein E-deleted vaccine strains of bovine herpesvirus type 1 |
| title_full | Establishment of a multiplex real-time PCR assay for the detection and differentiation of wild-type and glycoprotein E-deleted vaccine strains of bovine herpesvirus type 1 von Kerstin Wernike |
| title_fullStr | Establishment of a multiplex real-time PCR assay for the detection and differentiation of wild-type and glycoprotein E-deleted vaccine strains of bovine herpesvirus type 1 von Kerstin Wernike |
| title_full_unstemmed | Establishment of a multiplex real-time PCR assay for the detection and differentiation of wild-type and glycoprotein E-deleted vaccine strains of bovine herpesvirus type 1 von Kerstin Wernike |
| title_short | Establishment of a multiplex real-time PCR assay for the detection and differentiation of wild-type and glycoprotein E-deleted vaccine strains of bovine herpesvirus type 1 |
| title_sort | establishment of a multiplex real time pcr assay for the detection and differentiation of wild type and glycoprotein e deleted vaccine strains of bovine herpesvirus type 1 |
| topic | polymerase chain reaction cabt real time PCR cabt Bovine herpesvirus 1 cabt vaccines cabt glycoproteins cabt |
| topic_facet | polymerase chain reaction real time PCR Bovine herpesvirus 1 vaccines glycoproteins Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025247124&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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