Analysis of the anti-fibrotic effects of hop bitter acids on hepatic stellate cells and their anti-tumorigenic effects on hepatocellular carcinoma cells:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online-Zugang: | Volltext https://nbn-resolving.org/urn:nbn:de:bvb:355-epub-256071 Inhaltsverzeichnis |
| Beschreibung: | III, 85 S. Ill., graph. Darst. |
Internformat
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| 245 | 1 | 0 | |a Analysis of the anti-fibrotic effects of hop bitter acids on hepatic stellate cells and their anti-tumorigenic effects on hepatocellular carcinoma cells |c vorgelegt von Michael Sebastian Saugspier |
| 264 | 1 | |c 2012 | |
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Datensatz im Suchindex
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|---|---|
| adam_text | IMAGE 1
I
TABLE OF CONTENTS
1 SUMMARY 1
2 INTRODUCTION 3
2.1 BOTANY 3
2.2 HOP CHEMISTRY 5
2.2.1 LUPULIN INGREDIENTS 5
2.2.2 HOP BITTER ACIDS 6
2.3 BIOLOGICAL EFFECTS OF HOP BITTER ACIDS 8
2.3.1 ANTI-BACTERIAL EFFECTS 8
2.3.2 ANTI-INFLAMMATORY EFFECTS 9
2.3.3 ANTI-CANCER EFFECTS 12
2.3.4 EFFECTS ON LIPID AND CARBOHYDRATE METABOLISM 14
2.3.5 OTHER EFFECTS 16
2.4 TOXICOLOGY 16
2.5 EFFECTS OF HOP BITTER ACIDS ON THE LIVER 18
2.6 LIVER DISEASES 20
2.6.1 DEFINITION AND DEVELOPMENT 20
2.6.2 LIVER FIBROSIS 20
2.6.3 LIVER CANCER 21
2.7 AIM OF THE THESIS 23
3 MATERIALS AND METHODS 24
3.1 CHEMICALS AND REAGENTS 24
3.2 LABORATORY EXPENDABLES 25
3.3 LABORATORY INSTRUMENTS 25
3.4 CELL CULTURE 26
3.4.1 CELL CULTURE MEDIUM 26
3.4.2 CULTIVATION OF CELLS 27
3.4.3 DETERMINATION OF CELL NUMBER AND VIABILITY 27
3.4.4 CRYOPRESERVATION OF CELLS 28
3.4.5 HUMAN HEPATOCELLULAR CARCINOMA CELL LINES 28
3.4.6 ISOLATION OF PRIMARY HUMAN HEPATIC STELLATE CELLS 28
3.4.7 ISOLATION OF PRIMARY MURINE HEPATIC STELLATE CELLS 29
3.5 HOP BITTER ACID EXTRACTS 31
HTTP://D-NB.INFO/1026354757
IMAGE 2
TABLE OF CONTENTS LI
3.6 ENRICHMENT OF HOP BITTER ACIDS 32
3.7 PREPARATION OF A HOP BITTER ACID-SUPPLEMENTED CHOW FOR ORAL
APPLICATION 32
3.8 HOP BITTER ACID-RECOVERY FROM THE HOP BITTER ACID-SUPPLEMENTED
CHOW.. 33
3.9 THIN LAYER CHROMATOGRAPHY 33
3.10 RNA ANALYSIS 34
3.10.1 RNA ISOLATION 34
3.10.2 REVERSE TRANSCRIPTION PCR 34
3.10.3 QUANTITATIVE REAL TIME PCR 35
3.11 PROTEIN ANALYSIS 36
3.11.1 ISOLATION OF WHOLE CELL PROTEINS 36
3.11.2 DETERMINATION OF PROTEIN CONCENTRATION 37
3.11.3 WESTERN BLOT ANALYSIS 37
3.11.3.1 SDS PAGE GEL ELECTROPHORESIS 37
3.11.3.2 PROTEIN BLOT AND DETECTION 38
3.11.4 IMMUNOCYTOCHEMISTRY 40
3 . 1 1 . 5 QUANTIFICATION OF E R K 1 / 2 AND N F K B 4 0
3.11.6 AP-1 REPORTER GENE ASSAY 41
3.12 ANALYSIS OF CELL CULTURE SUPERNATANTS 41
3.13 FUNCTIONAL ASSAYS 42
3.13.1 PROLIFERATION ASSAY (XTT) 42
3.13.2 MIGRATION ASSAY 42
3.14 STATISTICAL ANALYSIS 43
4 RESULTS 44 -I
4.1 EFFECTS OF HOP BITTER ACIDS ON HEPATIC STELLATE CELLS 44
4.1.1 RATIONALE 44
4.1.2 IN VITRO ACTIVATION OF HSC 45
4.1.3 PROLIFERATION AND CELL VIABILITY 46
4 . 1 . 4 N F K B ACTIVITY AND PRO-INFLAMMATORY GENE EXPRESSION 4 9
4.1.5 SUMMARY 50
4.2 EFFECTS OF HOP BITTER ACIDS ON HEPATOCELLULAR CARCINOMA CELLS 51
4.2.1 RATIONALE 51
4.2.2 CELL VIABILITY 51
4.2.3 PROLIFERATION AND MIGRATORY POTENTIAL 53
4 . 2 . 4 E R K 1 / 2 , A P - 1 AND N F K B ACTIVITY 5 4
IMAGE 3
TABLE OF CONTENTS III
4.2.5 COMPARISON OF BITTER ACIDS AND REMNANT LIPOPHILIC COMPOUNDS 57
4.2.6 SUMMARY 57
4.3 BA-RECOVERY EXPERIMENTS FROM RODENT CHOW SUPPLEMENTED BA-EXTRACT. 58
5 DISCUSSION 61
5.1 HOP BITTER ACIDS AND HEPATIC INFLAMMATION AND FIBROSIS 61
5.2 HOP BITTER ACIDS AND HEPATOCELLULAR CARCINOMA 63
5.3 POOR RECOVERY OF HOP BITTER ACIDS IN ANIMAL CHOW SUPPLEMENTED WITH A
BITTER ACID-EXTRACT 64
5.4 CONCLUSION 66
6 REFERENCES 68
7 ABBREVIATIONS 77
8 APPENDIX 80
8.1 CURRICULUM VITAE 80
8.2 ADVANCED TRAINING COURSES 81
8.3 PUBLICATIONS 81
8.4 POSTER PRESENTATIONS 82
8.5 DANKSAGUNG 83
8.6 EIDESSTATTLICHE ERKIARUNG 85
|
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| discipline | Chemie / Pharmazie |
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| spelling | Saugspier, Michael Sebastian 1981- Verfasser (DE-588)1025160266 aut Analysis of the anti-fibrotic effects of hop bitter acids on hepatic stellate cells and their anti-tumorigenic effects on hepatocellular carcinoma cells vorgelegt von Michael Sebastian Saugspier 2012 III, 85 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Regensburg, Univ., Diss., 2012 Bitterstoff (DE-588)4202498-5 gnd rswk-swf Leber (DE-588)4034933-0 gnd rswk-swf Cytostatikum (DE-588)4068347-3 gnd rswk-swf Astrozyt (DE-588)4143257-5 gnd rswk-swf Hopfen Art (DE-588)4160640-1 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Leber (DE-588)4034933-0 s Astrozyt (DE-588)4143257-5 s Hopfen Art (DE-588)4160640-1 s Bitterstoff (DE-588)4202498-5 s Cytostatikum (DE-588)4068347-3 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:355-epub-256071 http://epub.uni-regensburg.de/25607/ Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:355-epub-256071 Resolving-System DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025220671&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Saugspier, Michael Sebastian 1981- Analysis of the anti-fibrotic effects of hop bitter acids on hepatic stellate cells and their anti-tumorigenic effects on hepatocellular carcinoma cells Bitterstoff (DE-588)4202498-5 gnd Leber (DE-588)4034933-0 gnd Cytostatikum (DE-588)4068347-3 gnd Astrozyt (DE-588)4143257-5 gnd Hopfen Art (DE-588)4160640-1 gnd |
| subject_GND | (DE-588)4202498-5 (DE-588)4034933-0 (DE-588)4068347-3 (DE-588)4143257-5 (DE-588)4160640-1 (DE-588)4113937-9 |
| title | Analysis of the anti-fibrotic effects of hop bitter acids on hepatic stellate cells and their anti-tumorigenic effects on hepatocellular carcinoma cells |
| title_auth | Analysis of the anti-fibrotic effects of hop bitter acids on hepatic stellate cells and their anti-tumorigenic effects on hepatocellular carcinoma cells |
| title_exact_search | Analysis of the anti-fibrotic effects of hop bitter acids on hepatic stellate cells and their anti-tumorigenic effects on hepatocellular carcinoma cells |
| title_full | Analysis of the anti-fibrotic effects of hop bitter acids on hepatic stellate cells and their anti-tumorigenic effects on hepatocellular carcinoma cells vorgelegt von Michael Sebastian Saugspier |
| title_fullStr | Analysis of the anti-fibrotic effects of hop bitter acids on hepatic stellate cells and their anti-tumorigenic effects on hepatocellular carcinoma cells vorgelegt von Michael Sebastian Saugspier |
| title_full_unstemmed | Analysis of the anti-fibrotic effects of hop bitter acids on hepatic stellate cells and their anti-tumorigenic effects on hepatocellular carcinoma cells vorgelegt von Michael Sebastian Saugspier |
| title_short | Analysis of the anti-fibrotic effects of hop bitter acids on hepatic stellate cells and their anti-tumorigenic effects on hepatocellular carcinoma cells |
| title_sort | analysis of the anti fibrotic effects of hop bitter acids on hepatic stellate cells and their anti tumorigenic effects on hepatocellular carcinoma cells |
| topic | Bitterstoff (DE-588)4202498-5 gnd Leber (DE-588)4034933-0 gnd Cytostatikum (DE-588)4068347-3 gnd Astrozyt (DE-588)4143257-5 gnd Hopfen Art (DE-588)4160640-1 gnd |
| topic_facet | Bitterstoff Leber Cytostatikum Astrozyt Hopfen Art Hochschulschrift |
| url | http://epub.uni-regensburg.de/25607/ https://nbn-resolving.org/urn:nbn:de:bvb:355-epub-256071 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025220671&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT saugspiermichaelsebastian analysisoftheantifibroticeffectsofhopbitteracidsonhepaticstellatecellsandtheirantitumorigeniceffectsonhepatocellularcarcinomacells |