Regulation of gene expression during embryonic development of Drosophila melanogaster:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Zsfassung in engl. und dt. Sprache |
| Beschreibung: | 187 S. Ill., graph. Darst. |
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| 245 | 1 | 0 | |a Regulation of gene expression during embryonic development of Drosophila melanogaster |c [presented by Jelena Erceg] |
| 246 | 1 | 3 | |a Genregulation während der Embryonalentwicklung der Fruchtfliege Drosophila melanogaster |
| 264 | 1 | |c 2012 | |
| 300 | |a 187 S. |b Ill., graph. Darst. | ||
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Datensatz im Suchindex
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TABLE OF CONTENTS
SUMMARY 1
ZUSAMMENFASSUNG 2
ABBREVIATIONS 4
1 INTRODUCTION 9
1.1 REGULATION OF GENE EXPRESSION 9
1.1.1 PROMOTERS, THE GENERAL TRANSCRIPTION FACTORS AND RNA POLYMERASE II
IN TRANSCRIPTION OF THE GENES 9
1.1.2 ACTIVITY OF TRANSCRIPTION FACTORS ON C/S-REGULATORY MODULES 11
1.1.3 CONTROL OF GENE EXPRESSION AT THE LEVEL OF CHROMATIN 2 1
1.1.4 POLYCOMB GROUP (PCG) PROTEINS MEDIATE REPRESSION 26
1.2 DROSOPHILA MESODERM DEVELOPMENT AS A MODEL TO STUDY GENE
REGULATION34 1.2.1 DORSAL-VENTRAL PATTERNING BY DORSAL GRADIENT IN
DROSOPHILA EMBRYO 34 1.2.2 SUBDIVISION OF THE MESODERM INTO FOUR DOMAINS
37
2 AIM 4 6
3 MATERIALS AND METHODS 4 7
3.1 MATERIALS 4 7
3.1.1 INSTRUMENTS 4 7
3.1.2 CHEMICALS AND REAGENTS 4 8
3.1.3 MISCELLANEOUS MATERIALS 50
3.1.4 OLIGONUCLEOTIDES 5 1
3.1.5 ANTIBODIES 5 1
3.1.6 PLASMID VECTORS 52
3.1.7 SOFTWARE 52
3.1.8 MEDIA, SOLUTIONS AND BUFFERS 52
3.1.9 FLY LINES 56
3.1.10 WEBSITES 59
3.2 METHODS 6 0
3.2.1 MOLECULAR BIOLOGY AND BIOCHEMISTRY 60
3.2.2 HISTOLOGICAL TECHNIQUES 63
3.2.3 ANALYSIS OF THE GENOME-WIDE SEQUENCING DATA 64
4 RESULTS 6 6
4.1 ASSESSING THE BASIC BUILDING BLOCKS NECESSARY TO GENERATE CIS-
REGULATORY MODULES (CRMS) THAT REGULATE SPATIO-TEMPORAL EXPRESSION 66
4.1.1 DISSECTION OF THE LOGIC FOR THE CRM ACTIVITY BY SYNTHETIC BIOLOGY
APPROACH 66 4.1.2 GENERATION OF EXPRESSION IN THE DORSAL MESODERM 70
4.1.3 GENERATION OF EXPRESSION IN THE AMNIOSEROSA 74
4.1.4 GENERATION OF EXPRESSION IN THE VISCERAL MESODERM 79
4.1.5 GENERATION OF EXPRESSION IN THE HEART 84
4.1.6 ROBUSTNESS OF CRM ACTIVITY 86
4.1.7 SUMMARY OF GENERAL RULES FOR CRM ACTIVITY IN THE VISCERAL MESODERM
AND HEART 9 3
4.2 THE ROLE AND DYNAMICS OF CHROMATIN SILENCING DURING MESODERM
DEVELOPMENT 9 6
4.2.1 EPIGENOMIC BORDERS BETWEEN HETEROCHROMATIN AND EUCHROMATIN IN T H
E DEVELOPING MESODERM 9 8
4.2.2 GENOME-WIDE DISTRIBUTION OF PHO OCCUPANCY IN THE MESODERM 102
4.2.3 THE ROLE OF PHO AT THE TRANSCRIPTION START SITE 105
HTTP://D-NB.INFO/1025837673
IMAGE 2
4.2.4 THE ROLE OF PHO A T DEVELOPMENTAL CRMS 109
5 DISCUSSION 1 1 7
5.1 SOME FACTORS HAVE MORE AUTONOMOUS REGULATORY POTENTIAL THAN OTHERS
117 5.2 SENSITIVITY TO MOTIF GRAMMAR IS TISSUE-SPECIFIC 119
5.3 THE DIVERSE INFLUENCES ON THE ROBUSTNESS O F THE CRM ACTIVITY 125
5.4 REFINED TISSUE-SPECIFIC RESOLUTION OF THE DYNAMICS IN THE CHROMATIN
SILENCING 128
5.4.1 INSIGHTS INTO THE ORGANIZATION OF CHROMATIN FROM THE DISTRIBUTIONS
OF H3K9ME2 AND H3K9ME3 128
5.4.2 INVOLVEMENT OF THE POLYCOMB GROUP PROTEINS (PCG) AROUND THE TSS IN
THE REGULATION OF GENE EXPRESSION 130
5.4.3 PHO-MEDIATED REGULATION OF THE CHROMATIN LANDSCAPE A T CRMS 132
5.4.4 AN INTEGRATIVE VIEW OF GENE REGULATION AT THE LEVEL OF THE NUCLEAR
ORGANIZATION 135
6 CONCLUSIONS AND FUTURE DIRECTIONS 1 3 7
7 APPENDIX 1 3 9
7.1 SEQUENCES OF CLONED SYNTHETIC CRMS 139
7.1.1 HOMOTYPIC SYNTHETIC CRMS 139
7.1.2 HETEROTYPIC SYNTHETIC CRMS 140
7.2 EXPRESSION PATTERNS O F THE SYNTHETIC CRMS DURING EMBRYOGENESIS 145
7.3 VALIDATION O F THE QUALITY FOR PHO BITS-CHLP-SEQ DATASETS 155
8 REFERENCES 1 5 8
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| any_adam_object | 1 |
| author | Erceg, Jelena |
| author_GND | (DE-588)1024991504 |
| author_facet | Erceg, Jelena |
| author_role | aut |
| author_sort | Erceg, Jelena |
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| bvnumber | BV040366797 |
| ctrlnum | (OCoLC)819522101 (DE-599)BSZ369813308 |
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| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.8615774 |
| dewey-search | 571.8615774 |
| dewey-sort | 3571.8615774 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Thesis Book |
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| indexdate | 2024-07-10T00:22:36Z |
| institution | BVB |
| language | English |
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| spelling | Erceg, Jelena Verfasser (DE-588)1024991504 aut Regulation of gene expression during embryonic development of Drosophila melanogaster [presented by Jelena Erceg] Genregulation während der Embryonalentwicklung der Fruchtfliege Drosophila melanogaster 2012 187 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in engl. und dt. Sprache Heidelberg, Univ., Diss., 2012 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025220503&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Erceg, Jelena Regulation of gene expression during embryonic development of Drosophila melanogaster |
| subject_GND | (DE-588)4113937-9 |
| title | Regulation of gene expression during embryonic development of Drosophila melanogaster |
| title_alt | Genregulation während der Embryonalentwicklung der Fruchtfliege Drosophila melanogaster |
| title_auth | Regulation of gene expression during embryonic development of Drosophila melanogaster |
| title_exact_search | Regulation of gene expression during embryonic development of Drosophila melanogaster |
| title_full | Regulation of gene expression during embryonic development of Drosophila melanogaster [presented by Jelena Erceg] |
| title_fullStr | Regulation of gene expression during embryonic development of Drosophila melanogaster [presented by Jelena Erceg] |
| title_full_unstemmed | Regulation of gene expression during embryonic development of Drosophila melanogaster [presented by Jelena Erceg] |
| title_short | Regulation of gene expression during embryonic development of Drosophila melanogaster |
| title_sort | regulation of gene expression during embryonic development of drosophila melanogaster |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025220503&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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