DNA repair protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | Undetermined |
| Veröffentlicht: |
New York [u.a.]
Humana Press
2012
|
| Ausgabe: | 3. ed. |
| Schriftenreihe: | Methods in molecular biology
920 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVII, 631 S. Ill., graph. Darst. |
| ISBN: | 9781617799976 |
Internformat
MARC
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| 245 | 1 | 0 | |a DNA repair protocols |c ed. by Lotte Bjergbæk |
| 250 | |a 3. ed. | ||
| 264 | 1 | |a New York [u.a.] |b Humana Press |c 2012 | |
| 300 | |a XVII, 631 S. |b Ill., graph. Darst. | ||
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Datensatz im Suchindex
| _version_ | 1804149408784187394 |
|---|---|
| adam_text | Contents
Preface
..............................................................
v
Contributors
.......................................................... xiii
1
DNA
Repair:
Front and Center
and Not Going Away!
...................... 1
Vilhelm
A. Bohr
Part I Identification of Repair Genes and Generation
of Repair Mutants
2
Identification of Genes Required for Damage Survival Using a Cell-Based
RNAi Screen Against the
Drosophila
Genome
............................. 9
Dashnamoorthy Ravi and Alexander James Roy Bishop
3
Isolating Genes Involved with Genotoxic Drug Response in the Nematode
Caenorhabditis
elegans
Using Genome-Wide RNAi Screening
................. 27
Lone
Vedel
Scheler,
Tine Horning
M0ller, Steffen Nergaard,
Lotte
Vestergaard, and Anders
Olsen
4
Establishment of the
DNA
Repair-Defective Mutants in DT40 Cells
............ 39
Masam
ichi Ishiai,
Emi
Uchida, and
Minoru Takata
Part II Assessing
DNA
Damage and Repair
5
Electrophoretic Mobility Shift Assays for Protein-DNA Complexes
Involved in
DNA
Repair
............................................. 53
Chun Tsai, Vaughn
Smider,
Byungjoon Hwang, and Gilbert
Chu
6
The Comet Assay: A Sensitive Genotoxicity Test for the Detection
of
DNA
Damage and Repair
.......................................... 79
Günter Speit
and Andreas Rothfuss
7
Detection of
DNA
Damage by Comet Fluorescence In Situ Hybridization
....... 91
Wiebke
Schlörmann
and Michael Glei
8
Quantitative
DNA
Damage and Repair Measurement
with the Yeast Comet Assay
.......................................... 101
Rui Oliveira
and
Björn
Johansson
9
Analysis of
DNA
Damage and Repair in Nuclear and Mitochondrial
DNA
of Animal Cells Using Quantitative PCR
............................
Ill
Amy M.
Furda,
Amanda Smith Bess, Joel
N.
Meyer,
and Bennett Van
Houten
IX
χ
Contents
Part III Repair of Incorrect or Damaged
DNA
Bases
10
In Vitro
DNA
Mismatch Repair in Human Cells
........................... 135
Liya Gu, Charles M. Ensor, and Guo-Min Li
11
Eukaryotic
DNA
Mismatch Repair In Vitro
.............................. 149
Fenghua Yuan, Limin Song, Fengsong Liu, Liya Gu, and Yanbin Zhang
12
Immuno-Slot Blot Assay for Detection of UVR-Mediated
DNA
Damage
........ 163
Mahsa Karbaschi, Nigel J. Brady, Mark D. Evans, and Marcus S. Cooke
13
Quantification of
DNA Photoproducts
in Mammalian Cell
DNA
Using
Radioimmunoassay
................................................. 177
Thomas
R.
Berton and David L. Mitchell
14
Measuring the Formation and Repair of UV Damage at the
DNA
Sequence Level by Ligation-Mediated PCR
.............................. 189
Ahmad Besaratinia and
Gerd
P.
Pfeifer
15
Construction of Plasmids Containing Site-Specific
DNA InterStrand
Cross-Links for Biochemical and Cell Biological Studies
..................... 203
Milica
Enoiu, The Vinh Ho, David T. Long, Johannes C. Walter,
and Orlando D.
Schärer
16
Replication-Coupled
DNA InterStrand
Cross-Link Repair
in Xenopus Egg Extracts
............................................. 221
Puck Knipscheer,
Markus Raschle,
Orlando
D.
Schärer,
and Johannes
С
Walter
17
In Vitro Base Excision Repair Using Mammalian Cell Extracts
................ 245
Jason L. Parsons and Grigory L. Dianov
18
Using Arabidopsis Cell Extracts to Monitor Repair of
DNA
Base
Damage In Vitro
.................................................. 263
Dolores
Córdoba-Canero,
Teresa
Roldán-Arjona,
and Rafael JR.. Ariza
19
In Vitro Monitoring of Base Excision Repair in Saccharomyces cerevisiae
......... 279
Simone Moertl
and
Fred Ahne
20
Mitochondrial Base Excision Repair Assays
............................... 289
Ricardo Gredilla
and Tinna Stevnsner
Part IV
DNA Strand
Breakage and Repair
21
O-Hydroxylamine-Coupled Alkaline Gel Electrophoresis Assay
for the Detection and Measurement of
DNA Single-Strand
Breaks
............. 307
April M. Luke and
Jun Nakamura
22 DNA Double-Strand
Break Damage and Repair Assessed by Pulsed-Field
Gel Electrophoresis
................................................. 315
Helen E. Bryant
23
Quantitative Amplification of Single-Stranded
DNA........................ 323
Eva-Maria
Holstein
and David Lydall
24
Simple,
Non
-radioactive Measurement of Single-Stranded
DNA
at Telomeric, Sub-telomeric, and Genomic Loci in Budding Yeast
.............. 341
James M. Dewar and David Lydall
Contents xi
25 Monitoring DNA
Recombination Initiated by
HO Endonuclease.............. 349
Neal Sugawara and James E. Haber
26 Monitoring I-Scel-Induced Double-Strand
Break Repair
in DT40
Cells.........
371
David du Plessis and Julian
E. Sale
27 I-Scel
-Based Assays to Examine Distinct Repair Outcomes
of Mammalian Chromosomal Double Strand Breaks
........................ 379
Amanda Gunn and Jeremy M. Stark
28
Studying Repair of a Single Protein-Bound Nick In Vivo Using
the Flp-Nick System
................................................ 393
Ida Nielsen,
Anni
H.
Andersen, and
Lotte
Bjergb&ek
29
Measurement of Diversification in the Immunoglobulin Light
Chain Gene of DT40 Cells
........................................... 417
Julian E. Sale
30
Live Cell Microscopy of
DNA
Damage Response in Saccharomyces cerevisiae
...... 433
Sonia
Silva,
Irene
Gallina,
Nadine Eckert-Boulet,
and Michael Lisby
31
Use of Gene Targeting to Study Recombination in Mammalian
Cell
DNA
Repair Mutants
........................................... 445
Jennifer J. Rahn, Gerald M. Adair, and Rodney S. Nairn
32
In Vitro Rejoining of Double Strand Breaks in Genomic
DNA................ 471
George Iliakis,
Emil
Mladenov, and Nge Cheong
33
An In Vitro
DNA Double-Strand
Break Repair Assay Based
on End-Joining of Defined Duplex Oligonucleotides
....................... 485
Kamal
Dotta,
Shubhadeep Purkayastha, Ronald D. Neumann,
and Thomas A. Winters
Part V
DNA
Damage Tolerance and Checkpoints
34
Assays of Bypass Replication of Genotoxic Lesions in Cell-Free Extracts
......... 503
Nana
Nikolaishvili-Feinberg and
Marila
Cordeiro-Stone
35
Quantitative Measurement of
Translesion DNA
Synthesis
in Mammalian Cells
................................................ 529
Omer Ziv, Noam
Diamant,
Sigal Shachar, Ayal
Hendel,
and
Zvi
Livneh
36
Detection of PCNA Modifications in Saccharomyces cerevisiae
................. 543
Adelina
A. Davies and
Helle D. Ulrich
37
In Vitro PCNA Modification Assays
.................................... 569
Joanne L· Parker and
Helle D. Ulrich
38
Analysis of Inhibition of
DNA
Replication in Irradiated Cells Using
the SV40 Based In Vitro Assay of
DNA
Replication
........................ 591
George Iliakis,
Emil
Mladenov, Ya Wang, and
Нощ
Tan Wang
39
CHK1 Kinase Activity Assay
.......................................... 603
Hong Tan Wang and
Ta Wang
40
Histone H2AX Phosphorylation: A Marker for
DNA
Damage
................ 613
Arishya Sharma,
Kamini
Singh, and
Alexandru
Almasan
Index
............................................................... 627
|
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | DNA repair protocols ed. by Lotte Bjergbæk 3. ed. New York [u.a.] Humana Press 2012 XVII, 631 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 920 Springer protocols DNS-Reparatur (DE-588)4150347-8 gnd rswk-swf Molekularbiologische Methode (DE-588)7650063-9 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content DNS-Reparatur (DE-588)4150347-8 s Molekularbiologische Methode (DE-588)7650063-9 s b DE-604 Bjergbæk, Lotte Sonstige oth Erscheint auch als Online-Ausgabe 978-1-61779-998-3 Methods in molecular biology 920 (DE-604)BV035362695 920 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025217270&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | DNA repair protocols Methods in molecular biology DNS-Reparatur (DE-588)4150347-8 gnd Molekularbiologische Methode (DE-588)7650063-9 gnd |
| subject_GND | (DE-588)4150347-8 (DE-588)7650063-9 (DE-588)4143413-4 |
| title | DNA repair protocols |
| title_auth | DNA repair protocols |
| title_exact_search | DNA repair protocols |
| title_full | DNA repair protocols ed. by Lotte Bjergbæk |
| title_fullStr | DNA repair protocols ed. by Lotte Bjergbæk |
| title_full_unstemmed | DNA repair protocols ed. by Lotte Bjergbæk |
| title_short | DNA repair protocols |
| title_sort | dna repair protocols |
| topic | DNS-Reparatur (DE-588)4150347-8 gnd Molekularbiologische Methode (DE-588)7650063-9 gnd |
| topic_facet | DNS-Reparatur Molekularbiologische Methode Aufsatzsammlung |
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