Expressions- und Funktionsanalyse des Kationenkanals TRPM1:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | German |
| Veröffentlicht: |
2012
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | VI, 79 S. Ill., graph. Darst. 21 cm |
Internformat
MARC
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| 100 | 1 | |a Dietz, Anna Stephanie |e Verfasser |0 (DE-588)1020796871 |4 aut | |
| 245 | 1 | 0 | |a Expressions- und Funktionsanalyse des Kationenkanals TRPM1 |c vorgelegt von Anna Stephanie Dietz |
| 264 | 1 | |c 2012 | |
| 300 | |a VI, 79 S. |b Ill., graph. Darst. |c 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Marburg, Univ., Diss., 2012 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025205838&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-025205838 | ||
Datensatz im Suchindex
| _version_ | 1804149388922060800 |
|---|---|
| adam_text | INHALTSVERZEICHNIS
ABBILDUNGSVERZEICHNIS
IV
TABELLENVERZEICHNIS
V
ABKUERZUNGSVERZEICHNIS
VI
1
EINLEITUNG
1
1.1
TRP-KANAELE............................................................................................................................................
1
1.2
TRPM-KANAELE
........................................................................................................................................
3
1.3
TRPM1
.................................
4
1.4
ZIELE
DIESER
ARBEIT
...............................................................................................................................
6
2
MATERIAL
UND
METHODEN
7
2.1
MATERIAL
................................................................................................................................................
7
2.1.1
ANTIKOERPER
.................................................................................................................................
7
2.1.2
CHEMIKALIEN
...............................................................................................................................
7
2.1.3
DATENBANKEN
UND
SOFTWARE
.....................................................................................................
8
2.1.4
ENZYME
UND
STANDARDS
.............................................................................................................
9
2.1.5
GERAETE
.......................................................................................................................................
9
2.1.6
MOLEKULARBIOLOGISCHE
REAGENZIENSYSTEME
............................................................................
9
2.1.7
OLIGONUKLEOTIDE
.......................................................................................................................
10
2.1.8
TIERE
UND
ZELLLINIEN
.................................................................................................................
11
2.1.9
VERBRAUCHSMATERIAL
................................................................................................................
11
2.1.10
ZELLKULTUR
..................................................................................................................................
11
2.2
METHODEN
...........................................................................................................................................
12
2.2.1
DNA-PRAEPARATION
UND
-ANALYSE
.............................................................................................
12
2.2.1.1
ALLGEMEINE
HANDHABUNG
VON
DNA
..........................................................................
12
2.2.1.2
POLYMERASEKETTENREAKTION
(PCR)
............................................................................
12
2.2.1.3
SUBKLONIERUNG
VON
PCR-AMPLIFIKATEN
.....................................................................
13
2.2.1.4
AMPLIFIKATION
VON
CDNA-ENDEN
(RACE)
..................................................................
13
2.2.1.5
ORTSGERICHTETE
MUTAGENESE
....................................................................................
15
2.2.2
RNA-PRAEPARATION
UND
-ANALYSE
.............................................................................................
16
2.2.2.1
ALLGEMEINE
HANDHABUNG
VON
RNA
..........................................................................
16
2.2.2.2
RNA-ISOLATION
AUS
ZELLEN
UND
GEWEBEN
.................................................................
16
2.2.2.3
AUFREINIGUNG
VON
MRNA
..........................................................................................
17
2.2.2.4
GELELEKTROPHORESE
VON
RNA....................................................................................
17
2.2.2.5
NORTHERN
BLOT
............................................................................................................
18
2.2.2.6
NORTHERN
HYBRIDISIERUNG
.........................................................................................
19
2.2.3
PROTEIN-PRAEPARATION
UND
-ANALYSE
..........................................................................................
19
2.2.3.1
SDS-POLYACRYLAMID-GELELEKTROPHORESE
(PAGE)
.....................................................
19
2.2.3.2
WESTERN
BLOT
............................................................................................................
21
2.2.3.3
HERSTELLUNG
VON
TRPMI-ANTIKOERPERN
.................
21
2.2.4
ZELLKULTUR
.................................................................................................................................
21
2.2.4.1
ALLGEMEINE
HANDHABUNG
VON
ZELLEN
......................................................................
21
2.2.4.2
TRANSFEKTION
VON
CDNA
...........................................................................................
22
2.2.5
HISTOLOGIE
................................................................................................................................
22
2.2.5.1
HERSTELLUNG
VON
GEFRIERSCHNITTEN
...........................................................................
22
2.2.5.2
IMMUNFAERBUNG
.........................................................................................................
22
2.2.5.3
X-GAL-FAERBUNG
...........................................................................................................
23
2.2.5.4
LICHT
UND
FLUORESZENZMIKROSKOPIE
........................................................................
24
2.2.6
TRPMI-KNOCKOUT-MAUS
..........................................................................................................
24
2.2.6.1
DNA-ISOLATION
AUS
GEWEBE
.....................................................................................
25
2.2.6.2
GENOTYPISIERUNG
DES
TRPMI-LOCUS
.......................................................................
25
2.2.6.3
GENOTYPISIERUNG
DES
AGOUTI-LOCUS
........................................................................
26
2.2.6.4
GENOTYPISIERUNG
DES
TYROSINASE-LOCUS
..................................................................
26
2.2.7
MESSUNG
INTRAZELLULAEREN
KALZIUMS
MIT
AEQUORIN
...................................................................
27
2.2.8
ELEKTROPHYSIOLOGIE
....................................................................................................................
28
3
ERGEBNISSE
30
3.1
EXPRESSIONSANALYSE
VON
TRPMI
IN
MAUSGEWEBEN
...........................................................................
30
3.1.1
MULTIPLE
TISSUE
NORTHERN
BLOT
...............................................................................................
30
3.1.2
IMMUNFAERBUNG
MIT
TRPMI-ANTIKOERPERN
..............................................................................
31
3.1.3
FAERBUNGEN
MIT
DEM
SUBSTRAT
X-GAL
........................................................................................
32
3.1.3.1
X-GAL-FAERBUNG
DES
AUGES
.........................................................................................
33
3.1.3.2
X-GAL-FAERBUNG
DER
HAUT
...........................................................................................
35
3.1.3.3
X-GAL-FAERBUNG
DES
GEHIRNS
......................................................................................
35
3.1.3.4
X-GAL-FAERBUNG
VON
EMBRYONEN
................................................................................
36
3.2
KLONIERUNG
DER
TRPML-CDNA
..............................................................................................................
38
3.2.1
KLONIERUNG
EINER
TRPML-CDNA
MITTELS
RT-PCR
UND
RACE
...................................................
38
3.2.2
MODIFIKATION
DER
TRPML-CDNA
...............................................................................................41
3.3
IN
VITRO
EXPRESSIONSANALYSE
.................................................................................................................
42
3.3.1
IN
VITRO
EXPRESSIONSANALYSE
DURCH
WESTERN
BLOT
.................................................................
42
3.3.2
SUBZELLULAERE
LOKALISATION
VON
TRPMI
IN
HEK-293-ZELLEN
....................................................
43
3.3.3
SUBZELLULAERE
LOKALISATION
VON
TRPMI
IN
B16-FL-ZELLEN
.......................................................
43
3.4
FUNKTIONSANALYSE
VON
TRPMI
............................................................................................................44
3.4.1
MESSUNG
DER
INTRAZELLULAEREN
KALZIUMKONZENTRATION
MIT
AEQUORIN
....................................
44
3.4.2
PATCH-CLAMP-EXPERIMENTE
.....................................................................................................47
3.5
DIE
TRPMI-KNOCKOUT-MAUS
..............................................................................................................49
3.5.1
HERSTELLUNGSSTRATEGIE
.............................................................................................................49
3.5.2
GENOTYPISIERUNG
.....................................................................................................................
50
3.5.3
PHAENOTYP
DER
TRPMI-KNOCKOUT-MAUS
..................................................................................
51
4
DISKUSSION
53
4.1
GEWEBSSPEZIFISCHES
EXPRESSIONSPROFIL
VON
TRPMI
.........................................................................
53
4.2
PHAENOTYP
DER
TRPMI-KNOCKOUT-MAUS
.............................................................................................
56
4.3
KLONIERUNG
VON
TRPML
.........................................................................................................................
58
4.4
IN
VITRO
EXPRESSIONSANALYSE
................................................................................................................
60
4.5
FUNKTIONSANALYSE
VON
TRPMI
............................................................................................................
61
5
ZUSAMMENFASSUNG
65
LITERATURVERZEICHNIS
69
VERZEICHNIS
AKADEMISCHER
LEHRER
78
DANKSAGUNG
79
|
| any_adam_object | 1 |
| author | Dietz, Anna Stephanie |
| author_GND | (DE-588)1020796871 |
| author_facet | Dietz, Anna Stephanie |
| author_role | aut |
| author_sort | Dietz, Anna Stephanie |
| author_variant | a s d as asd |
| building | Verbundindex |
| bvnumber | BV040351796 |
| ctrlnum | (OCoLC)812212684 (DE-599)DNB1021356085 |
| discipline | Medizin |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV040351796 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T00:22:15Z |
| institution | BVB |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-025205838 |
| oclc_num | 812212684 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | VI, 79 S. Ill., graph. Darst. 21 cm |
| publishDate | 2012 |
| publishDateSearch | 2012 |
| publishDateSort | 2012 |
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| spelling | Dietz, Anna Stephanie Verfasser (DE-588)1020796871 aut Expressions- und Funktionsanalyse des Kationenkanals TRPM1 vorgelegt von Anna Stephanie Dietz 2012 VI, 79 S. Ill., graph. Darst. 21 cm txt rdacontent n rdamedia nc rdacarrier Marburg, Univ., Diss., 2012 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025205838&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Dietz, Anna Stephanie Expressions- und Funktionsanalyse des Kationenkanals TRPM1 |
| subject_GND | (DE-588)4113937-9 |
| title | Expressions- und Funktionsanalyse des Kationenkanals TRPM1 |
| title_auth | Expressions- und Funktionsanalyse des Kationenkanals TRPM1 |
| title_exact_search | Expressions- und Funktionsanalyse des Kationenkanals TRPM1 |
| title_full | Expressions- und Funktionsanalyse des Kationenkanals TRPM1 vorgelegt von Anna Stephanie Dietz |
| title_fullStr | Expressions- und Funktionsanalyse des Kationenkanals TRPM1 vorgelegt von Anna Stephanie Dietz |
| title_full_unstemmed | Expressions- und Funktionsanalyse des Kationenkanals TRPM1 vorgelegt von Anna Stephanie Dietz |
| title_short | Expressions- und Funktionsanalyse des Kationenkanals TRPM1 |
| title_sort | expressions und funktionsanalyse des kationenkanals trpm1 |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025205838&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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