Alterations of brain dendrite and synapse structure by the Streptococcus pneumoniae neurotoxin pneumolysin - Insights and pharmacological modulation:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online-Zugang: | kostenfrei Inhaltsverzeichnis |
| Beschreibung: | VIII, 100 Bl. Ill., graph. Darst. |
Internformat
MARC
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| 100 | 1 | |a Wippel, Carolin |d 1981- |e Verfasser |0 (DE-588)1024502791 |4 aut | |
| 245 | 1 | 0 | |a Alterations of brain dendrite and synapse structure by the Streptococcus pneumoniae neurotoxin pneumolysin - Insights and pharmacological modulation |c von Carolin Wippel |
| 246 | 1 | 3 | |a Morphologische Veränderungen von Dendriten und Synapsen durch das Neurotoxin Pneumolysin - Einblicke und pharmakologischer Ansatz |
| 264 | 1 | |c 2012 | |
| 300 | |a VIII, 100 Bl. |b Ill., graph. Darst. | ||
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| 502 | |a Würzburg, Univ., Diss., 2012 | ||
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| 650 | 0 | 7 | |a Dendrit |0 (DE-588)4123047-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Bakterielle Hirnhautentzündung |0 (DE-588)4246316-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Streptococcus pneumoniae |0 (DE-588)4244508-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Synapse |0 (DE-588)4184224-8 |2 gnd |9 rswk-swf |
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| 689 | 0 | 0 | |a Streptococcus pneumoniae |0 (DE-588)4244508-5 |D s |
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| 912 | |a ebook | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-025183333 | ||
Datensatz im Suchindex
| _version_ | 1804149359878602752 |
|---|---|
| adam_text | IMAGE 1
INDEX
TABLE OF FIGURES I
INDEX OF TABLES ILL
LIST OF ABBREVIATIONS IV
PUBLICATIONS VIII
1 ABSTRACT 9
ENGLISH 9
DEUTSCH 11
2 INTRODUCTION 14
2.1 STREPTOCOCCUS PNEUMONIAE 14
2.1.1 VIRULENCE FACTORS 15
2.2 BACTERIAL MENINGITIS CAUSED BY STREPTOCOCCUS PNEUMONIAE 16
2.2.1 EPIDEMIOLOGY 16
2.2.2 PATHOGENESIS 18
2.2.3 DIAGNOSIS AND TREATMENT 20
2.3 PNEUMOLYSIN 2 1
2.3.1 CONTRIBUTION OF PLY T O THE VIRULENCE OF S. PNEUMONIAE 22
2.3.2 PORE FORMATION PROCESS 23
2.1 CELLULAR COMPONENTS O F NERVOUS TISSUE 24
2.1.1 NEURONS 24
2.1.2 NEUROGLIA 26
2.1.3 THE GLUTAMATE-GLUTAMINE CYCLE 27
2.2 EXCITOTOXICITY 29
2.2.1 GLUTAMATE RECEPTORS 30
2.2.1.1 NMDA RECEPTORS 30
2.2.2 INHIBITORS 3 1
2.2.2.1 MK-801 3 1
2.2.2.2 AP5 32
3 METHODS 33
3.1 PNEUMOLYSIN EXPRESSION AND PURIFICATION 33
HTTP://D-NB.INFO/1025914031
IMAGE 2
INDEX
3.2 CELL CULTURE 33
3.2.1 PRIMARY NEURONAL CULTURE 33
3.2.2 PRIMARY ASTROCYTIC CULTURE 34
3.3 BRAIN SLICE CULTURE 35
3.3.1 THEORY 35
3.3.2 PREPARATION 35
3.4 TRANSFECTION O F PRIMARY NEURONS 36
3.4.1 THEORY 36
3.4.2 PROCEDURE 37
3.4.2.1 MULTIPLICATION O F THE PLASMID 38
3.5 CYTOTOXICITY ASSAY 38
3.5.1 THEORY 38
3.5.2 PROCEDURE 39
3.6 GLUTAMATE ASSAY 40
3.6.1 THEORY 40
3.6.2 PROCEDURE 40
3.7 CONFOCAL IMAGING O F BRAIN SLICES 4 1
3.7.1 DIL STAINING 42
3.7.1.1 PROCEDURE 42
3.7.2 IMMUNOHISTOCHEMISTRY 42
3.8 CALCIUM IMAGING 43
3.8.1 THEORY 43
3.8.2 PROCEDURE 43
3.9 STATISTICS AND ANALYSIS 44
4 MATERIALS 45
4.1 LABORATORY EQUIPMENT 45
4.2 BUFFERS AND SOLUTIONS 49
5 RESULTS 52
5.1 LIVE CELL IMAGING O F PRIMARY NEURONS 52
5.1.1 SUB-LYTIC AMOUNTS OF PNEUMOLYSIN INDUCE MORPHOLOGICAL CHANGES IN
PRIMARY NEURONS 52
5.2 ESTABLISHING AN ACUTE BRAIN SLICE CULTURE 55
5.2.1 C57BL/6 MICE (P7) BRAIN SLICES 57
5.2.2 C57BL/6 MICE (PL4) BRAIN SLICES 59
IMAGE 3
INDEX
5.2.2.1 CTRL VS. PLY 59
5.2.3 CELL CULTURE CONDITIONS MODULATING THE TOXICITY OF PNEUMOLYSIN 62
5.2.4 BRAIN SLICES OF MICE WITH PNEUMOCOCCAL MENINGITIS 63
5.2.4.1 DELTA6 MUTANT 65
5.2.4.2 MK-801 - ROLE IN PRIMARY NEURONAL CULTURE 65
5.2.4.3 MK-801 - ROLE IN BRAIN SLICE CULTURE 66
5.2.4.4 AP5 BRAIN SLICE EXPERIMENTS 67
5.3 GLUTAMATE MEASUREMENT 69
5.4 CALCIUM IMAGING 7 1
6 DISCUSSION 74
7 CONCLUDING REMARKS AND PERSPECTIVES 83
8 REFERENCES 84
ACKNOWLEDGMENT 95
CURRICULUM VITAE 96
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| spelling | Wippel, Carolin 1981- Verfasser (DE-588)1024502791 aut Alterations of brain dendrite and synapse structure by the Streptococcus pneumoniae neurotoxin pneumolysin - Insights and pharmacological modulation von Carolin Wippel Morphologische Veränderungen von Dendriten und Synapsen durch das Neurotoxin Pneumolysin - Einblicke und pharmakologischer Ansatz 2012 VIII, 100 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Würzburg, Univ., Diss., 2012 Bakteriengift (DE-588)4130547-4 gnd rswk-swf Dendrit (DE-588)4123047-4 gnd rswk-swf Bakterielle Hirnhautentzündung (DE-588)4246316-6 gnd rswk-swf Streptococcus pneumoniae (DE-588)4244508-5 gnd rswk-swf Synapse (DE-588)4184224-8 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Streptococcus pneumoniae (DE-588)4244508-5 s Bakteriengift (DE-588)4130547-4 s Dendrit (DE-588)4123047-4 s Synapse (DE-588)4184224-8 s Bakterielle Hirnhautentzündung (DE-588)4246316-6 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:20-opus-72016 https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-72016 Resolving-System kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025183333&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Wippel, Carolin 1981- Alterations of brain dendrite and synapse structure by the Streptococcus pneumoniae neurotoxin pneumolysin - Insights and pharmacological modulation Bakteriengift (DE-588)4130547-4 gnd Dendrit (DE-588)4123047-4 gnd Bakterielle Hirnhautentzündung (DE-588)4246316-6 gnd Streptococcus pneumoniae (DE-588)4244508-5 gnd Synapse (DE-588)4184224-8 gnd |
| subject_GND | (DE-588)4130547-4 (DE-588)4123047-4 (DE-588)4246316-6 (DE-588)4244508-5 (DE-588)4184224-8 (DE-588)4113937-9 |
| title | Alterations of brain dendrite and synapse structure by the Streptococcus pneumoniae neurotoxin pneumolysin - Insights and pharmacological modulation |
| title_alt | Morphologische Veränderungen von Dendriten und Synapsen durch das Neurotoxin Pneumolysin - Einblicke und pharmakologischer Ansatz |
| title_auth | Alterations of brain dendrite and synapse structure by the Streptococcus pneumoniae neurotoxin pneumolysin - Insights and pharmacological modulation |
| title_exact_search | Alterations of brain dendrite and synapse structure by the Streptococcus pneumoniae neurotoxin pneumolysin - Insights and pharmacological modulation |
| title_full | Alterations of brain dendrite and synapse structure by the Streptococcus pneumoniae neurotoxin pneumolysin - Insights and pharmacological modulation von Carolin Wippel |
| title_fullStr | Alterations of brain dendrite and synapse structure by the Streptococcus pneumoniae neurotoxin pneumolysin - Insights and pharmacological modulation von Carolin Wippel |
| title_full_unstemmed | Alterations of brain dendrite and synapse structure by the Streptococcus pneumoniae neurotoxin pneumolysin - Insights and pharmacological modulation von Carolin Wippel |
| title_short | Alterations of brain dendrite and synapse structure by the Streptococcus pneumoniae neurotoxin pneumolysin - Insights and pharmacological modulation |
| title_sort | alterations of brain dendrite and synapse structure by the streptococcus pneumoniae neurotoxin pneumolysin insights and pharmacological modulation |
| topic | Bakteriengift (DE-588)4130547-4 gnd Dendrit (DE-588)4123047-4 gnd Bakterielle Hirnhautentzündung (DE-588)4246316-6 gnd Streptococcus pneumoniae (DE-588)4244508-5 gnd Synapse (DE-588)4184224-8 gnd |
| topic_facet | Bakteriengift Dendrit Bakterielle Hirnhautentzündung Streptococcus pneumoniae Synapse Hochschulschrift |
| url | https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-72016 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025183333&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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