Molecular and ecological investigations of Caribbean sponge diseases:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | German |
| Veröffentlicht: |
2011
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| Schlagworte: | |
| Online-Zugang: | Volltext Inhaltsverzeichnis |
| Beschreibung: | CD u.d.T.: Underwater movies |
| Beschreibung: | 171 Bl. Ill. CD-ROM (12 cm) |
Internformat
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| 245 | 1 | 0 | |a Molecular and ecological investigations of Caribbean sponge diseases |c von Hilde Gabriele Angermeier |
| 246 | 1 | 3 | |a Molekulare und ökologische Untersuchungen zu Krankheiten Karibischer Meeresschwämme |
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Datensatz im Suchindex
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| adam_text | IMAGE 1
TABLE OF CONTENTS
TABLE OF CONTENTS
I. SUMMARY 1
ZUSAMMENFASSUNG 3
II. INTRODUCTION 5
1. MARINE SPONGES - THE PHYLUM PORIFERA 5
1.1 TAXONOMY 5
1.2 MORPHOLOGY 6
2. SPONGES AND MICROORGANISMS 11
3. SYMBIOTIC INTERACTIONS 15
4. AIMS 17
III. MATERIAL AND METHODS 18
1. EPIDEMIOLOGY O F DISEASE 18
1.1 THE MARINE SPONGE XESTOSPONGIA MUTA AS A MODEL SYSTEM 18
1.2 SPONGE ORANGE BAND DISEASE AFFECTING X. MUTA 19
1.3 THE MARINE SPONGE AMPHIMEDON COMPRESSA AS A MODEL SYSTEM 21
1.4 SPONGE WHITE PATCH DISEASE AFFECTING A. COMPRESSA 21
2. SPONGE COLLECTION 23
3. BINOCULAR MICROSCOPY 23
4. LIGHT MICROSCOPY 23
5. SCANNING ELECTRON MICROSCOPY 24
6. TRANSMISSION ELECTRON MICROSCOPY 24
7. EPIFLUORESCENCE MICROSCOPY 25
8. MICROBIAL CULTIVATION 25
8.1 SPONGE ORANGE BAND DISEASE AND ASSOCIATED BACTERIA 27
8.2 SPONGE WHITE PATCH DISEASE AND ASSOCIATED BACTERIA , 28
HTTP://D-NB.INFO/1026700388
IMAGE 2
TABLE OF CONTENTS
9. RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP) 29
10. DNA EXTRACTION AND DENATURING GRADIENT GEL ELECTROPHORESIS 30
11. CLONING AND SEQUENCING 32
12. PHYLOGENETIC ANALYSIS 34
13. GENBANK ACCESSION NUMBERS O F NUCLEOTIDE SEQUENCES 34
14. CHLOROPHYLL A ANALYSIS VIA SPECTROPHOTOMETRY 35
15. HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY 35
16. PATHOGENICITY TRIALS 36
16.1 INFECTION EXPERIMENTS ON SPONGE ORANGE BAND DISEASE 36
16.2 INFECTION EXPERIMENTS ON SPONGE WHITE PATCH DISEASE 37
IV. RESULTS 39
1. SPONGE ORANGE BAND DISEASE OF XESTOSPONGIA MUTA 39
1.1 UNDERWATER OBSERVATIONS 39
1.2 MARINE INVERTEBRATES ASSOCIATED WITH X. MUTA 41
1.3 HISTOLOGICAL INVESTIGATIONS O F SPONGE ORANGE BAND DISEASE 42
1.4 ELECTRON MICROSCOPICAL OBSERVATIONS 43
SCANNING ELECTRON MICROSCOPY 43
TRANSMISSION ELECTRON MICROSCOPY , 48
THE ROLE OFX. MUTA S CYANOBACTERIAL SYMBIONTS 51
1.5 CELL COUNTS ON X MUTA TISSUES 53
1.6 MICROBIAL CULTIVATION 56
BACTERIAL DIVERSITY O F BAHAMIAN SPONGES 56
BACTERIAL DIVERSITY O F SPONGES FROM THE FLORIDA KEYS 56
1.7 PROFILING MICROBIAL COMMUNITY CHANGES BY DGGE 59
THE BACTERIAL COMMUNITY DURING SPONGE ORANGE BAND DISEASE 59
THE CYANOBACTERIAL COMMUNITY DURING SPONGE ORANGE BAND DISEASE 61
1.8 PHYLOGENETIC ANALYSIS 65
IMAGE 3
TABLE O F CONTENTS
1.9 CHEMICAL ECOLOGY 67
PROFILING O F SECONDARY METABOLITES VIA HPLC 67
PROFILING O F CHLOROPHYLL A VIA SPECTROPHOTOMETRY 69
1.10 INFECTION EXPERIMENTS 71
2. SPONGE WHITE PATCH DISEASE OF AMPHIMEDON COMPRESSA 73
2.1 VISUAL DOCUMENTATION O F SPONGE WHITE PATCH DISEASE 73
2.2 DISEASE ECOLOGY 74
2.3 HISTOLOGICAL INVESTIGATIONS 76
BINOCULAR MICROSCOPY 76
LIGHT MICROSCOPY 77
2.4 ELECTRON MICROSCOPICAL OBSERVATIONS 78
SCANNING ELECTRON MICROSCOPY 78
TRANSMISSION ELECTRON MICROSCOPY 80
2.5 MICROBIAL CULTIVATION 83
BACTERIAL DIVERSITY O F SPONGE WHITE PATCH DISEASE IN WINTER 2007 83
ISOLATION O F COLLAGEN DEGRADING BACTERIA 85
BACTERIAL DIVERSITY O F SPONGE WHITE PATCH DISEASE IN SPRING 2009 85
2.6 PROFILING MICROBIAL COMMUNITY CHANGES BY DGGE 87
THE BACTERIAL COMMUNITY O F HEALTHY A. COMPRESSA 87
THE BACTERIAL COMMUNITY O F SPONGE WHITE PATCH DISEASE 88
2.7 INFECTION EXPERIMENTS ON SPONGE WHITE PATCH DISEASE 94
2.8 THE PRESENCE O F ACCESSORY SPONGE DISEASES 95
V. DISCUSSION 96
1. MICROBIAL PATHOGENS O F CORALS AND SPONGES 96
2. THE PATHOLOGY O F SPONGE ORANGE BAND DISEASE AFFECTING XESTOSPONGIA
MUTA 100
2.1 VISUAL OBSERVATIONS ON SPONGE ORANGE BAND DISEASE 100
IMAGE 4
TABLE O F CONTENTS
2.2 THE MICROBIOLOGY O F SPONGE ORANGE BAND DISEASE 102
2.3 CONCLUSIONS 104
3. THE PATHOLOGY O F SPONGE WHITE PATCH DISEASE AFFECTING AMPHIMEDON
COMPRESSA ... 105
3.1 ECOLOGICAL AND VISUAL OBSERVATIONS ON SPONGE WHITE PATCH DISEASE 105
3.2 THE MICROBIOLOGY O F SPONGE WHITE PATCH DISEASE 105
3.3 CONCLUSIONS 109
4. CONSENSUS 110
VI. OUTLOOK 112
VII. REFERENCES 113
VIII. ANNEX 131
1. TABLES 1 TO 7 131
2. ABBREVIATIONS, ACRONYMS & SYMBOLS 140
3. EQUIPMENT & GENERAL LABORATORY SUPPLY 143
4. CHEMICAL REAGENTS 149
5. SOLUTIONS & BUFFERS 152
6. MEDIA 158
7. ENZYMES & KITS 160
8. OLIGONUCLEOTIDE PRIMERS 161
9. MARKERS 162
10. VECTORS & MICROORGANISMS 163
11. APPLIED SOFTWARE 165
IX. PUBLICATIONS 166
X. SYMPOSIA 167
XI. WORKSHOPS 169
XII. SUMMER ACADEMIES 170
XIII. FIELD WORK 170
XIV. CURRICULUM VITAE 171
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| spelling | Angermeier, Hilde Gabriele 1979- Verfasser (DE-588)1024416216 aut Molecular and ecological investigations of Caribbean sponge diseases von Hilde Gabriele Angermeier Molekulare und ökologische Untersuchungen zu Krankheiten Karibischer Meeresschwämme Underwater movies 2011 171 Bl. Ill. CD-ROM (12 cm) txt rdacontent n rdamedia nc rdacarrier CD u.d.T.: Underwater movies Würzburg, Univ., Diss., 2011 Meeresschwämme (DE-588)4307227-6 gnd rswk-swf Pathogene Bakterien (DE-588)4173543-2 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Meeresschwämme (DE-588)4307227-6 s Pathogene Bakterien (DE-588)4173543-2 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:20-opus-56855 https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-56855 Resolving-System kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025177795&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Angermeier, Hilde Gabriele 1979- Molecular and ecological investigations of Caribbean sponge diseases Meeresschwämme (DE-588)4307227-6 gnd Pathogene Bakterien (DE-588)4173543-2 gnd |
| subject_GND | (DE-588)4307227-6 (DE-588)4173543-2 (DE-588)4113937-9 |
| title | Molecular and ecological investigations of Caribbean sponge diseases |
| title_alt | Molekulare und ökologische Untersuchungen zu Krankheiten Karibischer Meeresschwämme Underwater movies |
| title_auth | Molecular and ecological investigations of Caribbean sponge diseases |
| title_exact_search | Molecular and ecological investigations of Caribbean sponge diseases |
| title_full | Molecular and ecological investigations of Caribbean sponge diseases von Hilde Gabriele Angermeier |
| title_fullStr | Molecular and ecological investigations of Caribbean sponge diseases von Hilde Gabriele Angermeier |
| title_full_unstemmed | Molecular and ecological investigations of Caribbean sponge diseases von Hilde Gabriele Angermeier |
| title_short | Molecular and ecological investigations of Caribbean sponge diseases |
| title_sort | molecular and ecological investigations of caribbean sponge diseases |
| topic | Meeresschwämme (DE-588)4307227-6 gnd Pathogene Bakterien (DE-588)4173543-2 gnd |
| topic_facet | Meeresschwämme Pathogene Bakterien Hochschulschrift |
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