Plant tissue culture: techniques and experiments
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Academic Press
2013
|
| Ausgabe: | 3. ed. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVI, 188 S. Ill. |
| ISBN: | 9780124159204 0124159206 |
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Datensatz im Suchindex
| _version_ | 1804149325036519424 |
|---|---|
| adam_text | IMAGE 1
PLANT TISSUE CULTURE
TECHNIQUES AND EXPERIMENTS 311 -
THIRD EDITION
ROBERTA H. SMITH
EMERITUS PROFESSOR
DEPARTMENT OF HORTICULTURE VEGETABLE CROPS IMPROVEMENT CENTER TEXAS A&M
UNIVERSITY COLLEGE STATION, TEXAS
AMSTERDAM * BOSTON * HEIDELBERG * LONDON NEW YORK * OXFORD * PARIS * SAN
DIEGO SAN FRANCISCO * SINGAPORE * SYDNEY * TOKYO
ACADEMIC PRESS IS AN IMPRINT OF ELSEVIER
IMAGE 2
PREFACE XI
ACKNOWLEDGMENTS XIII
SCHEDULING AND INTERRELATIONSHIPS OF EXERCISES XV
1. HISTORY OF PLANT CELL CULTURE 1
TREVOR A. THORPE
INTRODUCTION 1
THE EARLY YEARS 2
THE ERA OF TECHNIQUES DEVELOPMENT 3
THE RECENT PAST 5
CELL BEHAVIOR 6
PLANT MODIFICATION AND IMPROVEMENT 7
PATHOGEN-FREE PLANTS AND GERMPLASM STORAGE 9
CLONAL PROPAGATION 9
PRODUCT FORMATION 9
THE PRESENT ERA 10
2. SETUP OF A TISSUE CULTURE LABORATORY 23
WORK AREAS 24
MEDIA PREPARATION/CULTURE EVALUATION/RECORD-KEEPING AREA 24
ASEPTIC TRANSFER AREA 24
ENVIRONMENTALLY CONTROLLED CULTURE AREA 25
R
3. MEDIA COMPONENTS AND PREPARATION 31
INORGANIC SALTS 32
PLANT GROWTH REGULATORS 32
VITAMINS 36
CARBOHYDRATES 36
HEXITOLS 37
GELLING AGENT 37
AMINO ACIDS 38
ANTIBIOTICS 39
NATURAL COMPLEXES 39
MEDIA PH 40
MEDIUM PREPARATION 40
IMAGE 3
CONTENTS
4. EXPLANT PREPARATION 45
EXPLANT AGE 46
SEASON 46
EXPLANT SIZE 46
PLANT QUALITY 46
COAL 46
GENOTYPE 48
ASEPTIC TECHNIQUE 48
ASEPTIC GERMINATION OF SEEDS 49
CARROT, SUNFLOWER, BROCCOLI, COTTON, OR ANY SEED OF CHOICE 49
DOUGLAS FIR 50
5. CONTAMINATION 53
EXPLANT 53
DISINFECTING AGENTS 54
EXPLANT SOURCE 56
INTERNAL MICROBIAL CONTAMINATION 56
EXPLANT PRETREATMENT TO CONTROL MICROBIAL CONTAMINANTS 57
PERSONNEL 57
LAMINAR AIR FLOW HOOD 58
INSECTS 58
CONTROL OF INSECT CONTAMINATION 58
MEDIA 59
INSTRUMENTS 60
ROOM AIR HANDLING SYSTEM 61
6. CALLUS INDUCTION 63
CALLUS INITIATION 63
EXPLANT PREPARATION 65
CULTURE 66
OBSERVATIONS 66
DATA AND QUESTIONS 66
EXPLANT ORIENTATION R 67
EXPLANT 67.
OBSERVATIONS 68
ESTABLISHMENT OF COMPETENT CEREAL CELL CULTURES 69
EXPLANTS 70
DATA AND QUESTIONS 70
SALT SELECTION IN VITRO 70
EXPLANTS 71
DATA AND QUESTIONS 72
GROWTH CURVES 72
COTTON 73
TOBACCO 74
CE U AR V AV VAWON FAOM CAWUS CVAUITES 7 6
IMAGE 4
CONTENTS
VII
EXPLANT 76
DATA AND QUESTIONS 76
7. REGENERATION AND MORPHOGENESIS 81
CONTROLLED MORPHOGENESIS: POTATO TUBERIZATION 82
EXPLANT 83
DATA AND QUESTIONS 84
SOMATIC EMBRYOGENESIS 84
EXPLANT PREPARATION 86
PROCEDURE 86
DATA AND QUESTIONS 86
REGENERATION OF RICE 87
REGENERATION 87
DATA AND QUESTIONS 87
DORMANCY REQUIREMENTS OF EXPLANTS 87
EXPLANT PREPARATION 88
QUESTIONS 89
PRIMARY MORPHOGENESIS: DOUGLAS FIR 89
EXPLANT PREPARATION 89
DATA AND QUESTIONS 90
8. WOODY SHRUBS AND TREES 93
BRENT H. MCCOWN
RHODODENDRONS AND AZALEAS 95
EXPLANTS 97
ADVANCED STUDIES 97
ROSES 97
EXPLANT 98
ADVANCED STUDIES 98
BIRCH TREES 98
EXPLANT 98
ADVANCED STUDIES 99
WHITE CEDAR (ARBORVITAE) 99
EXPLANT 99
9. HAPLOID PLANTS FROM ANTHER CULTURE 103
DATURA ANTHER CULTURE 105
EXPLANT PREPARATION 105
QUESTIONS 106
AFRICAN VIOLET 106
EXPLANT PREPARATION 107
OBSERVATIONS 107
ROOT TIP CHROMOSOME SQUASH TECHNIQUE 107
QUESTIONS 108
TOBACCO 108
IMAGE 5
VIII CONTENTS
EXPLANT PREPARATION 108
OBSERVATIONS 109
QUESTIONS 109
ANTHER SQUASH TECHNIQUE 109
10. EMBRYO RESCUE 113
SWEET CORN EMBRYO CULTURE 114
PREPARATION AND CULTURE OF CORN EMBRYOS 11 5
QUESTIONS 116
CRABAPPLE AND PEAR 116
EXPLANT PREPARATION 116
OBSERVATIONS 11 6
QUESTIONS 117
11. MERISTEM CULTURE FOR VIRUS-FREE PLANTS 119
ISOLATION OF THE SHOOT APICAL MERISTEM 121
EXPLANT PREPARATION 121
QUESTIONS 122
ESTABLISHING VIRUS- AND BACTERIA-FREE PLANTS 122
EXPLANT PREPARATION 122
GARLIC PROPAGATION FROM BULB SCALES 123
EXPLANT PREPARATION 123
DATA 123
12. IN VITRO PROPAGATION FOR COMMERCIAL PRODUCTION OF
ORNAMENTALS 127
BOSTON FERN 129
STAGE I 130
STAGE II 131
STAGE III 132
STAGHORN FERN 133
STAGE I 134
STAGE II 134
STAGE III 135
FICUS 136
STAGE I 136
STAGE II 137
STAGE III 137
KALANCHOE 137
EXPLANT PREPARATION 138
DATA AND QUESTIONS 138
AFRICAN VIOLET 139
EXPLANT PREPARATION 139
DATA AND QUESTIONS 140
ENDANGERED SPECIES 140
IMAGE 6
CONTENTS
IX
PITCHER PLANTS 141
CACTUS 141
EXPLANT PREPARATION 142
13. PROTOPLAST ISOLATION AND FUSION 147
JUNGEUN KIM PARK, SUNGHUN PARK AND JAMES E. CRAVEN
PLANT PROTOPLAST ISOLATION AND FUSION 148
SUPPLIES 148
PROTOPLAST LIBERATION 149
PROTOPLAST PURIFICATION 150
VITAL STAINING 151
PROTOPLAST FUSION 151
YIELD DETERMINATION 151
DEVELOPING A PROTOPLAST ISOLATION PROTOCOL 152
14. AGROBACTERIUM-SAEDIATED TRANSFORMATION OF PLANTS 155
JUNGEUN KIM PARK, SUNGHUN PARK, QINGYU WU AND STUART SPRAGUE
PETUNIA OR TOBACCO LEAF DISK 157
SEQUENCE 157
PRECULTURE 157
EXPLANT PREPARATION 157
COCULTIVATION WITH AGROBACTERIUM 1 58
SHOOT TRANSFORMATION AND ELIMINATION OF AGROBACTERIUM 158
ROOTING AND PRETESTING FOR TRANSFORMED SHOOTS 159
PETUNIA SHOOT APEX 160
SEQUENCE 160
SEED GERMINATION 160
SHOOT INDUCTION 161
SHOOT APEX TRANSFORMATION 161
ROOTING 161
TOBACCO LEAF INFILTRATION 162
SEQUENCE 162
GROW AGROBACTERIUM 162
CULTURE AGROBACTERIUM 163
HARVEST AGROBACTERIUM 163
INFILTRATION TOBACCO LEAVES 163
APPENDICES 167
INDEX 183
|
| any_adam_object | 1 |
| author | Smith, Roberta H. |
| author_facet | Smith, Roberta H. |
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| ctrlnum | (OCoLC)802626462 (DE-599)BVBBV040305705 |
| discipline | Biologie Agrarwissenschaft Pflanzenbau |
| edition | 3. ed. |
| format | Book |
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| isbn | 9780124159204 0124159206 |
| language | English |
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| spelling | Smith, Roberta H. Verfasser aut Plant tissue culture techniques and experiments Roberta H. Smith 3. ed. Amsterdam [u.a.] Academic Press 2013 XVI, 188 S. Ill. txt rdacontent n rdamedia nc rdacarrier Plant tissue culture Gewebekultur (DE-588)4157245-2 gnd rswk-swf Pflanzen (DE-588)4045539-7 gnd rswk-swf Zellkultur (DE-588)4067547-6 gnd rswk-swf Pflanzenzelle (DE-588)4115551-8 gnd rswk-swf Pflanzenzelle (DE-588)4115551-8 s Zellkultur (DE-588)4067547-6 s DE-604 Pflanzen (DE-588)4045539-7 s Gewebekultur (DE-588)4157245-2 s HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025160633&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Smith, Roberta H. Plant tissue culture techniques and experiments Plant tissue culture Gewebekultur (DE-588)4157245-2 gnd Pflanzen (DE-588)4045539-7 gnd Zellkultur (DE-588)4067547-6 gnd Pflanzenzelle (DE-588)4115551-8 gnd |
| subject_GND | (DE-588)4157245-2 (DE-588)4045539-7 (DE-588)4067547-6 (DE-588)4115551-8 |
| title | Plant tissue culture techniques and experiments |
| title_auth | Plant tissue culture techniques and experiments |
| title_exact_search | Plant tissue culture techniques and experiments |
| title_full | Plant tissue culture techniques and experiments Roberta H. Smith |
| title_fullStr | Plant tissue culture techniques and experiments Roberta H. Smith |
| title_full_unstemmed | Plant tissue culture techniques and experiments Roberta H. Smith |
| title_short | Plant tissue culture |
| title_sort | plant tissue culture techniques and experiments |
| title_sub | techniques and experiments |
| topic | Plant tissue culture Gewebekultur (DE-588)4157245-2 gnd Pflanzen (DE-588)4045539-7 gnd Zellkultur (DE-588)4067547-6 gnd Pflanzenzelle (DE-588)4115551-8 gnd |
| topic_facet | Plant tissue culture Gewebekultur Pflanzen Zellkultur Pflanzenzelle |
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