Cellulases:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2012
|
| Ausgabe: | 1. ed. |
| Schriftenreihe: | Methods in enzymology
510 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | LII, 500 S., [5] Bl. Ill., graph. Darst. |
| ISBN: | 9780124159310 |
Internformat
MARC
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| 020 | |a 9780124159310 |c hbk |9 978-0-12-415931-0 | ||
| 035 | |a (OCoLC)802503249 | ||
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| 245 | 1 | 0 | |a Cellulases |c ed. by Harry J. Gilbert |
| 250 | |a 1. ed. | ||
| 264 | 1 | |a Amsterdam [u.a.] |b Elsevier, Acad. Press |c 2012 | |
| 300 | |a LII, 500 S., [5] Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in enzymology |v 510 | |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-025136665 | ||
Datensatz im Suchindex
| _version_ | 1804149290520543232 |
|---|---|
| adam_text | Contents
Contributors
xiii
Preface
xxi
Volumes in Series
xxiii
1.
Measurement of
em/o-l^-ß-Glucanase 1
Barry V. McCleary, Vincent McKie, and Anna
Draga
1.
Introduction
2
2.
Purity of EG Enzymes Included in the Study
2
3.
Polysaccharide and Oligosaccharide Substrates
3
4.
Viscometric Assay of EG Activity
4
5.
Reducing-Sugar Assays for EG Activity
6
6.
Soluble Chromogenic Substrates
10
7.
Insoluble Chromogenic Substrates
14
8.
Summary
15
References
16
2.
Biomass Conversion Determined via Fluorescent
Cellulose Decay Assay
19
Bente
Wischmann,
Marianne Toft, Marco
Malten,
and
K. C. McFarland
1.
Introduction
20
2.
Correlation Between Cellulose Decay Monitored by HPLC and
by Fluorescence
21
3.
Assay Development and Modeling of Response
Using Statistics
24
4.
Final Assay Procedure
32
Acknowledgment
36
References
36
3.
The Analysis of Saccharification in Biomass Using an
Automated High-Throughput Method
37
Caragh Whitehead, Leonardo D. Gomez, and Simon J. McQueen-Mason
1.
Introduction
38
2.
Automatic Saccharification Platform
39
vi
Contents
3.
Enzyme Characterization
44
4.
Conduding Remarks
49
Acknowledgments
49
References
49
4.
Studies of Enzymatic Cleavage of Cellulose Using
Polysaccharide Analysis by Carbohydrate gel
Electrophoresis (PACE)
51
Ondrej
Kosík,
Jennifer
R.
Bromley,
Marta
Busse-Wicher, Zhinong
Zhang,
and Paul Dupree
1.
Introduction
52
2.
The Principle of PACE
54
3.
Discussion: Other Possible Applications
64
4.
Summary
65
Acknowledgments
65
References
65
5.
Measuring Processivity
69
Svein
].
Horn, Morten S0rlie,
Kjell
M.
Vårum,
Priit
Väljamäe,
and
Vincent
G. H. Eijsink
1.
Introduction
70
2.
Classical Methods for Measuring Processivity
71
3.
Learning from Family
18
Chitinases
76
4.
Methods Based on Labeling—Apparent Versus
Intrinsic Processivity
80
5.
Concluding Remarks
89
Acknowledgments
90
References
90
6.
Distinguishing Xyloglucanase Activity in
entfo-ßil—
>4)glucanases
97
Jens M.
Eklöf,
Marcus
C. Ruda,
and Harry Brumer
1.
Introduction
98
2.
Analytical Tools for Xyloglucan Oligo- and Polysaccharide
Analysis
101
3.
Substrate Preparation
105
4.
Determination of Specific Xyloglucan Activity in a Glucanase
109
5.
Coda: Xyloglucan Hydrolysis by
ß-glucanases—
Obvious
but Often Overlooked
116
Acknowledgments
116
References
117
Contents
vii
7.
Methods for Structural Characterization of the Products
of Cellulose- and Xyloglucan-Hydrolyzing Enzymes
121
Maria J.
Peña,
Sami
T.
Tuomivaara, Breeanna
R.
Urbanowicz,
Malcolm A. O Neill, and William S. York
1.
Introduction
122
2.
Preparation of Substrates
124
3.
Purification of the Oligosaccharide Products
124
4.
Chemical and Structural Analysis of the Reaction Products
127
Acknowledgments
137
References
138
8.
The Crystallization and Structural Analysis of Cellulases
(and Other Glycoside Hydrolases): Strategies and Tactics
141
Shirley M. Roberts and Gideon
).
Davies
1.
General Crystallization Considerations
144
2.
Optimization and Additive Screens
149
3.
Cellulase- and Glycosidase-Specific Complex Tactics
152
4.
Buffer and Cryo-protection Problems
159
5.
Toward a Cellulase-Specific Approach?
162
Acknowledgments
162
References
163
9.
Visualization of Cellobiohydrolase I from Trichoderma reesei
Moving on Crystalline Cellulose Using High-Speed Atomic
Force Microscopy
169
Kiyohiko Igarashi, Takayuki Uchihashi,
Anu Koivula,
Masahisa
Wada,
Satoshi Kimura,
Merja Penttilä,
Toshio
Ando,
and Masahiro Samejima
1.
Introduction
170
2.
Sample Preparation for AFM Observations
171
3.
Observation of Cellulase Molecules on Crystalline Cellulose
174
4.
Image Analysis
177
5.
Conclusion
179
Acknowledgments
180
References
180
10.
Small-Angle X-ray Scattering and Crystallography: A Winning
Combination for Exploring the Multimodular Organization of
Cellulolytic Macromolecular Complexes
183
Mirjam Czjzek,
Henri-Pierre Fierobe, and
Véronique Receveur-Bréchot
1.
Introduction
184
2.
Measuring and Analyzing SAXS Data
186
viii Contents
3.
Combining SAXS and Crystallography to Analyze Multimodular
Organization of Cellulolytic Enzymes and Complexes
191
4.
Conclusions and Outlook
203
Acknowledgments
205
References
205
11.
Quantitative Approaches to The Analysis of
Carbohydrate-Binding Module Function
211
D. Wade Abbott and Alisdair
B. Boraston
1.
Introduction
212
2.
Experimental Approaches to Investigating CBM Function
215
3.
Summary and New Directions for CBM Research
228
References
229
12.
In Situ Detection of Cellulose with Carbohydrate-Binding
Modules
233
J. Paul Knox
1.
Introduction
234
2.
Preparation of Plant Materials
236
3.
CBM-Labeling Procedures
240
4.
Summary
244
Acknowledgment
244
References
244
13.
Interactions Between Family
3
Carbohydrate Binding
Modules (CBMs) and Cellulosomal Linker Peptides
247
Oren
Yaniv, Felix Frolow, Maly Levy-Assraf, Raphael Lamed, and
Edward A. Bayer
1.
Introduction
248
2.
Enzyme-Linked Immunosorbent Assay
(ELISA)
to Check
Possible Interactions Between CBM3 and Cellulosomal Linkers
250
3.
Isothermal
Titration Calorimetry
for Analysis of Interactions
Between CBM3 and Cellulosomal Linkers
253
Acknowledgments
258
References
258
14.
Approaches for Improving Thermostability Characteristics in
Cellulases
261
Michael Anbar and Edward A. Bayer
1.
Construction of Cellulase Libraries
262
2.
Screening Endoglucanases for Enhanced Thermostability
265
Contents
¡χ
3.
Screening
ß-Glucosidase
for Enhanced Thermostability
267
4.
In Vitro Recombination Between Best Thermostable Mutants
269
References
271
15.
Thermophilic Glycosynthases for Oligosaccharides
Synthesis
273
Beatrice Cobucci-Ponzano, Giuseppe
Perugino,
Andrea Strazzulli,
Mose
Rossi, and Marco Moracci
1.
Introduction
274
2.
Thermophilic
ß-Glycosynthases
from GH1
276
3.
Thermophilic a-L-Fucosyntases from
G
H29
285
4.
Thermophilic a-o-Galactosynthase from GH36
292
5.
Summary
295
Acknowledgments
296
References
296
16.
Engineering Cellulase Activity into Clostridium
acetobutyllcum
301
Henri-Pierre Fierobe, Florence Mingardon, and
Angélique Chanal
1.
Introduction
302
2.
Electrotransformation of
С
acetobutylicum ATCC824 and
Storage of
Recombinant
Strains
303
3.
Detection/Quantification of the Secreted Heterologous
Cellulosomal Protein
306
4.
Recombinant
Strain of
С
acetobutylicum Secreting a
Heterologous Minicellulosome
308
5.
Secretion Issue and the Use of Carrier Modules
311
6.
Outlook
314
Acknowledgments
315
References
315
17.
Transformation of Clostridium Thermocellum
by Electroporation
317
Daniel G. Olson and Lee R. Lynd
1.
Introduction
318
2.
Materials
318
3.
Methods
320
4.
Troubleshooting
328
References
329
χ
Contents
18.
Genetic and Functional Genomic Approaches for the
Study of Plant Cell Wall Degradation in Cellvibrio japonicus
331
Jeffrey G. Gardner and David H. Keating
1.
Introduction
332
2.
Growth and Maintenance of C. ¡aponicus
333
3.
Genetic Manipulation of
С
japonicus
336
4.
Construction of Mutations
338
5.
Transcriptomic Analysis of C. ¡aponicus
341
6.
Global Expression Profiling
342
7.
Summary and Future Directions
345
Acknowledgment
345
References
345
19.
Methods for the Isolation of Cellulose-Degrading
Microorganisms
349
James E. McDonald, David J. Rooks, and Alan J. McCarthy
1.
Introduction
350
2.
Cellulosic Substrates
351
3.
Isolation and Cultivation of Cellulolytic Microorganisms
354
4.
Methods for the Detection of Cellulose Degradation in Cultures
366
5.
Future Prospects
368
Acknowledgments
369
References
369
20.
Metagenomic Approaches to the Discovery of Cellulases
375
David J. Rooks, James E. McDonald, and Alan J. McCarthy
1.
Introduction
376
2.
Nucleic Acid Extraction from Environmental Samples
377
3.
Metagenomic Libraries
379
4.
Metatranscriptomics
385
5.
Outlook
389
Acknowledgment
390
References
390
21.
Escherichia
coli
Expression, Purification, Crystallization, and
Structure Determination of Bacterial Cohesin-Dockerin Complexes
395
Joana
L.
A. Brás,
Ana Luisa
Carvalho,
Aldino Viegas, Shabir Najmudin,
Victor
D. Alves,
José A.
M.
Prates,
Luís
Μ. Α.
Ferreira,
Maria J.
Romão,
Harry J. Gilbert,
and Carlos
M. G.A. Fontes
1.
Introduction
396
2.
Cloning of Cohesin and Dockerin Genes in Prokaryotic
Expression Vectors
397
Contents
x¡
3. Expression
and Purification of Cohesin-Dockerin
Complexes in
£
coli
400
4.
The Dual Binding Mode and the Crystallization of
Cohesin-Dockerin Complexes
402
5.
X-ray Crystallography of Cohesin-Dockerin Complexes
402
6.
Summary
413
Acknowledgments
414
References
414
22.
Measurements of Relative Binding of Cohesin and
Dockerin Mutants Using an Advanced
ELISA
Technique
for High-Affinity Interactions
417
Michal
Slutzki, Yoav Barak, Dan Reshef,
Ora Schueler-Furman,
Raphael Lamed, and Edward A. Bayer
1.
Introduction
418
2.
Preparation of Constructs for
ELISA
Procedure
420
3.
Detection of Free Dockerins Using
¡ELISA
422
4.
Data Analysis
425
5.
Notes
426
6.
Summary
427
References
427
23.
Designer Cellulosomes for Enhanced Hydrolysis of
Cellulosic Substrates
429
Yael Vazana, Sarah
Moraïs, Yoav
Barak, Raphael Lamed, and
Edward A. Bayer
1.
Construction of Designer Cellulosomes
430
2.
Scaffoldin Testing and Analysis of Cohesin-Dockerin
Interaction
433
3.
Chimeric Enzyme Testing
441
4.
Sugar Analysis
449
5.
Summary
450
References
451
24.
High-Throughput Screening of Cohesin Mutant Libraries
on Cellulose Microarrays
453
Michal
Slutzki,
Vered
Ruimy, Ely Morag, Yoav Barak, Rachel Haimovitz,
Raphael Lamed, and Edward A. Bayer
1.
Introduction
454
2.
Cohesin Mutants: Library Preparation
456
3.
Screening Small Cohesin Libraries
456
4.
Screening Large Cohesin Libraries
458
5.
Microarray Probing
459
x¡¡
Contents
6. DNA
Extraction
460
7. Notes 461
8.
Summary
461
References
462
Author Index
465
Subject Index
489
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| id | DE-604.BV040281312 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T00:20:41Z |
| institution | BVB |
| isbn | 9780124159310 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-025136665 |
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| series | Methods in enzymology |
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| spelling | Cellulases ed. by Harry J. Gilbert 1. ed. Amsterdam [u.a.] Elsevier, Acad. Press 2012 LII, 500 S., [5] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in enzymology 510 Cellulasen (DE-588)4147450-8 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Cellulasen (DE-588)4147450-8 s b DE-604 Gilbert, Harry J. Sonstige oth Methods in enzymology 510 (DE-604)BV000000938 510 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025136665&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Cellulases Methods in enzymology Cellulasen (DE-588)4147450-8 gnd |
| subject_GND | (DE-588)4147450-8 (DE-588)4143413-4 |
| title | Cellulases |
| title_auth | Cellulases |
| title_exact_search | Cellulases |
| title_full | Cellulases ed. by Harry J. Gilbert |
| title_fullStr | Cellulases ed. by Harry J. Gilbert |
| title_full_unstemmed | Cellulases ed. by Harry J. Gilbert |
| title_short | Cellulases |
| title_sort | cellulases |
| topic | Cellulasen (DE-588)4147450-8 gnd |
| topic_facet | Cellulasen Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025136665&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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