The response of growth and primary metabolism to a mild but sustained nitrogen limitation in Arabidopsis thaliana:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2009
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | VIII, 176 S. Ill., graph. Darst. |
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Datensatz im Suchindex
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| adam_text | IMAGE 1
TABLE O F CONTENTS
TABLE OF CONTENTS
DECLARATION . I
ACKNOWLEDGEMENTS II
ABBREVIATIONS I L L
SUMMARY VI
TABLE OF CONTENTS VII
1 INTRODUCTION 1
1.1 IMPORTANCE OF NITROGEN FOR PLANT GROWTH 1
1.1.1 THE NATURAL NITROGEN CYCLE 1
1.1.2 SOIL - THE PRIMARY MEDIUM FOR GROWTH O F TERRESTRIAL PLANTS 2
1.1.3 N-FERTILIZER INPUT AND RECENT ADVANCES IN IMPROVING NITROGEN USE
EFFICIENCY 2 1.2 N-UPTAKE 5
1.2.1 GENERAL 5
1.2.2 NITRATE UPTAKE 5
1.2.3 AMMONIUM UPTAKE 6
1.2.4 OTHER SOURCES O F N IN NATURAL SOILS 6
1.3 N-ASSIMILATION 7
1.3.1 NITRATE ASSIMILATION 7
1.3.2 AMMONIUM ASSIMILATION 9
1.3.3 AMINO ACID BIOSYNTHESIS 12
1.3.4 INFLUENCE O F N-ASSIMILATION ON CYTOSOLIC PH 13
1.3.5 CELLULAR BIOSYNTHESES REQUIRING N 15
1.3.6 N- ALLOCATION 15
1.4 PHYSIOLOGICAL AND METABOLIC RESPONSES TO N-LIMITATION 17
1.4.1 VISIBLE SYMPTOMS O F N-DEFICIENCY 17
1.4.2 METABOLIC CHANGES IN RESPONSE TO N-DEFICIENCY 17
1.5 POTENTIAL N-SENSING AND -SIGNALLING SYSTEMS 18
1.5.1 N-SIGNALLING METABOLITES 18
1.5.2 TRANSCRIPTION FACTORS INVOLVED IN N-SIGNALLING 19
1.5.3 PHYTOHORMONES 20
1.6 EXPERIMENTAL SYSTEMS TO STUDY N-LIMITATION 21
1.6.1 GENERAL OVERVIEW AND DEFINITIONS 21
1.6.2 ARTIFICIAL GROWTH SYSTEMS THAT PROVIDE N-LIMITATION 21
1.6.3 SOIL BASED SYSTEMS TO PROVIDE N-LIMITATION 22
1.7. POTENTIAL APPROACHES TO DISSECT NUE 23
1.7.1 CURRENT STATE O F RESEARCH CONCERNING NUE 23
VII
HTTP://D-NB.INFO/1026688973
IMAGE 2
TABLE O F CONTENTS
1.7.2 EXPERIMENTAL APPROACHES TO ADDRESS NUE 24
1.8 AIM OF THE THESIS 26
2 MATERIAL AND METHODS 27
2.1 PLANT MATERIAL 27
2.2 PLANT GROWTH 27
2.2.1 SOIL TYPES AND ANALYSES 27
2.2.2 GROWTH CONDITIONS 28
2.2.3 EXPERIMENTAL DESIGN 28
2.3 DETERMINATION OF WATER CONTENT AND LEAF AREA 29
2.4 REAGENTS 29
2.5 ELEMENTAL ANALYSES AND DETERMINATION O F TOTAL ION CONTENT 29
2.6 METABOLITE EXTRACTIONS 29
2.7 METABOLITE MEASUREMENTS 30
2.8 ENZYME MEASUREMENTS 31
2.8.1 GENERAL 31
2.8.2 CONTINUOUS ASSAYS 31
2.8.3 DISCONTINUOUS ASSAY 31
2.8.4 SIMPLE DISCONTINUOUS ASSAY 33
2.9 STATISTICAL ANALYSES 33
3 RESULTS 34
3.1 ESTABLISHMENT OF A SOIL-BASED GROWTH SYSTEM THAT PROVIDES MILD
N-LIMITATION 34 3.1.1 A SOIL MIX THAT PROVIDES SUSTAINED N-LIMITATION
DURING VEGETATIVE DEVELOPMENT 35
3.1.2 EXCLUSION O F POTENTIAL AMMONIUM TOXICITY 39
3.1.3 COMPLEMENTATION O F GROWTH BY SUPPLYING AMMONIUM NITRATE 39
3.1.4 ANALYSIS O F TOTAL ELEMENTAL COMPOSITION 41
3.1.5 DETERMINATION O F A PHOTOPERIOD LEADING TO MILD N-LIMITATION AND A
REDUCTION O F SHOOT BIOMASS O F 50 % 42
3.1.6 DOES THE SOIL SYSTEM ALSO PROVIDE N-LIMITATION IN OTHER SPECIES 43
3.1.7 CONCLUSIONS AND FURTHER AIMS 44
3.2 PHYSIOLOGICAL CHARACTERIZATION OF THE LOW NITROGEN GAUNTLET IN
ARABIDOPSIS COL-0 45
3.2.1 VISUAL CHARACTERIZATION 45
3.2.2 CHARACTERIZATION THROUGHOUT VEGETATIVE STAGES O F DEVELOPMENT 46
3.2.3 CHARACTERIZATION THROUGHOUT THE DIURNAL CYCLE 53
3.3 THE USE OF NATURAL VARIATION IN ARABIDOPSIS TO STUDY MILD
N-LIMITATION COMPARED TO N-REPLETE CONDITIONS 63
3.3.1 OBJECTIVES 63
VIII
IMAGE 3
TABLE O F CONTENTS
3.3.2 EFFECTS O F N-SUPPLY ON FW, METABOLITES AND ENZYMES WITHIN A
GENETICALLY DIVERSE SET O F ARABIDOPSIS GENOTYPES 63
3.3.3 CORRELATION ANALYSES 70
3.3.4 COMBINED MULTIVARIATE AND PRINCIPAL COMPONENT ANALYSIS 78
3.3.5 NITROGEN USE EFFICIENCY (NUE) 81
3.3.6 IDENTIFICATION O F EXTREME PERFORMING GENOTYPES 83
3.3.7 COMPARISON O F THE RESULTS WITH SIMILAR DATASETS 90
4 DISCUSSION 94
4.1 MILD AND SUSTAINED N-LIMITATION GENERATED BY A SOIL-BASED GROWTH
SYSTEM 95 4.2 MILD N-LIMITATION CONFIRMS KNOWN AND REVEALS PREVIOUSLY
UNKNOWN RESPONSES OF GROWTH AND C/N- METABOLISM IN ARABIDOPSIS COL-0 96
4.2.1 N-ASSIMILATION AND CENTRAL C-METABOLISM IS MODULATED BY N 96
4.2.2 AMINO ACIDS AND GDH ACTIVITY INCREASE UNDER MILD N-LIMITATION 98
4.2.3 SECONDARY METABOLISM IS UNAFFECTED UNDER MILD N-LIMITATION 101
4.3 MILD N-LIMITATION MODULATES GROWTH AND C/N- METABOLISM IN A
GENETICALLY DIVERSE SET OF ARABIDOPSIS THALIANA ACCESSIONS 102
4.3.1 THE IMPACT O F N ON TRAIT VARIABILITY IS BIGGER THAN THE GENETIC
EFFECT 102 4.3.2 MILD N-LIMITATION LEADS TO COORDINATED CHANGES O F THE
METABOLIC CORRELATION NETWORK 103
4.3.3 NUE AND EXTREME PERFORMING ACCESSIONS 110
4.4 LOW N GROWTH SYSTEMS ARE ONLY COMPARABLE ON A LIMITED SCALE 114
4.5 ACCLIMATION O F GROWTH AND METABOLISM TO A MILD AND SUSTAINED
N-LIMITATION IN ARABIDOPSIS 114
4.6 POTENTIAL SIGNALLING MECHANISMS INVOLVED IN THE ADJUSTMENT TO MILD
NLIMITATION 115
4.7 OUTLOOK AND PERSPECTIVES 117
5 REFERENCES 119
APPENDICES 136
APPENDIX 1 136
APPENDIX II 138
APPENDIX III 144
APPENDIX IV 151
APPENDIX V 156
APPENDIX VI 168
APPENDIX VII 171
APPENDIX VIII 173
APPENDIX IX 176
IX
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| discipline | Biologie |
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| spelling | Tschoep, Hendrik Verfasser aut The response of growth and primary metabolism to a mild but sustained nitrogen limitation in Arabidopsis thaliana von Hendrik Tschoep 2009 VIII, 176 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Potsdam, Univ., Diss., 2009 Stickstoffstoffwechsel (DE-588)4138777-6 gnd rswk-swf Ackerschmalwand (DE-588)4141299-0 gnd rswk-swf Nährstoffmangel (DE-588)4345147-0 gnd rswk-swf Pflanzenwachstum (DE-588)4045595-6 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Ackerschmalwand (DE-588)4141299-0 s Stickstoffstoffwechsel (DE-588)4138777-6 s Nährstoffmangel (DE-588)4345147-0 s Pflanzenwachstum (DE-588)4045595-6 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025128998&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Tschoep, Hendrik The response of growth and primary metabolism to a mild but sustained nitrogen limitation in Arabidopsis thaliana Stickstoffstoffwechsel (DE-588)4138777-6 gnd Ackerschmalwand (DE-588)4141299-0 gnd Nährstoffmangel (DE-588)4345147-0 gnd Pflanzenwachstum (DE-588)4045595-6 gnd |
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| title | The response of growth and primary metabolism to a mild but sustained nitrogen limitation in Arabidopsis thaliana |
| title_auth | The response of growth and primary metabolism to a mild but sustained nitrogen limitation in Arabidopsis thaliana |
| title_exact_search | The response of growth and primary metabolism to a mild but sustained nitrogen limitation in Arabidopsis thaliana |
| title_full | The response of growth and primary metabolism to a mild but sustained nitrogen limitation in Arabidopsis thaliana von Hendrik Tschoep |
| title_fullStr | The response of growth and primary metabolism to a mild but sustained nitrogen limitation in Arabidopsis thaliana von Hendrik Tschoep |
| title_full_unstemmed | The response of growth and primary metabolism to a mild but sustained nitrogen limitation in Arabidopsis thaliana von Hendrik Tschoep |
| title_short | The response of growth and primary metabolism to a mild but sustained nitrogen limitation in Arabidopsis thaliana |
| title_sort | the response of growth and primary metabolism to a mild but sustained nitrogen limitation in arabidopsis thaliana |
| topic | Stickstoffstoffwechsel (DE-588)4138777-6 gnd Ackerschmalwand (DE-588)4141299-0 gnd Nährstoffmangel (DE-588)4345147-0 gnd Pflanzenwachstum (DE-588)4045595-6 gnd |
| topic_facet | Stickstoffstoffwechsel Ackerschmalwand Nährstoffmangel Pflanzenwachstum Hochschulschrift |
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