Sphingosine-1-phosphate: methods and protocols
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York
Humana Press
2012
|
| Schriftenreihe: | Methods in molecular biology
874 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | xi, 221 p. ill |
Internformat
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Datensatz im Suchindex
| _version_ | 1804149258578821120 |
|---|---|
| adam_text | Titel: Sphingosine-1-phosphate
Autor: Pébay, Alice
Jahr: 2012
Contents
Preface............................................................... v
Contributors........................................................... ix
1 Biochemical Methods for Quantifying Sphingolipids: Ceramide, Sphingosine,
Sphingosine Kinase-1 Activity, and Sphingosine-1-Phosphate.............. 1
Leyre Brizuela and Olivier Cuvillier
2 Isoform-Selective Assays for Sphingosine Kinase Activity.................. 21
Melissa R. Pitman, Duyen H. Pham, and Stuart M. Pitson
3 Quantification of Sphingosine-1-Phosphate and Related Sphingolipids
by Liquid Chromatography Coupled to Tandem Mass Spectrometry......... 33
Constantin Bode and Markus H. Gräler
4 A Cleanup Method for Mass Spectrometry of Sphingosine-1-Phosphate
in Blood and Solid Tissues Using a Phosphate Capture Molecule........... 45
Jun-ichi Morisbige, Tamotsu Tanaka, and Kiyoshi Satouchi
5 Immunohistochemical Detection of Sphingosine-1-Phosphate
and Sphingosine Kinase-1 in Human Tissue Samples..................... 55
Barbara Visentin, Gary Reynolds, and Roger Sabbadini
6 Assessment of Sphingosine-1-Phosphate Activity in Biological Samples
by Receptor Internalization and Adherens Junction Formation............. 69
Hideru Obinata and Timothy Hla
7 High-Throughput Assays to Measure Intracellular Ca2* Mobilization
in Cells that Express Recombinant SIP Receptor Subtypes................ 77
William J. Valentine and Gabor Tigyi
8 Quantifying Sphingosine-1-Phosphate-Dependent Activation
of the RhoGTPases.............................................. 89
Matt D. Medlin, Joan M. Taylor, and Christopher P. Mack
9 Optimized Protocols to Analyze Sphingosine-1-Phosphate Signal
Transduction Pathways During Acrosomal Exocytosis in Human Sperm...... 99
Silvia A. Belmonte and Laila Suhaiman
10 Use of Intravital Microscopy and In Vitro Chemotaxis Assays
to Study the Roles of Sphingosine-1-Phosphate in Bone Homeostasis........ 129
Taeko Ishii, Shunsuke Kawamura, Issei Nishiyama, Junichi Kikuta,
and Masaru Ishii
11 Assessment of Sphingosine-1-Phosphate Receptor Expression
and Associated Intracellular Signaling Cascades in Primary Cells
of the Human Central Nervous System............................... 141
Véronique E. Miron, Bryce A. Durafourt, Jack P. Antel,
and Timothy E. Kennedy
VII
viii Contents
12 Sphingosine-1-Phosphate Signaling in Skeletal Muscle Cells............... 155
Elisabetta Meacci, Francesca Bini, and Chiara Battistini
13 Maintenance of Human Embryonic Stem Cells by Sphingosine-1-Phosphate
and Platelet-Derived Growth Factor................................. 167
Raymond C.B. Wong, Martin F. Pera, and Alice Pébay
14 Ceramide and Sphingosine-1-Phosphate Signaling in Embryonic Stem Cell
Differentiation................................................. 177
Erbard Bieberich
15 Sphingosine-1-Phosphate Signaling in Neural Progenitors................. 193
Phillip Callihan and Shelley B. Hooks
16 Utilizing Sphingosine-1-Phosphate to Stimulate Sprouting Angiogenesis...... 201
Shih-Chi Su and KaylaJ. Bayless
Index................................................................ 275
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| indexdate | 2024-07-10T00:20:10Z |
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| language | English |
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| physical | xi, 221 p. ill |
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| series | Methods in molecular biology |
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| spelling | Pébay, Alice Verfasser aut Sphingosine-1-phosphate methods and protocols ed. by Alice Pébay ... New York Humana Press 2012 xi, 221 p. ill txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 874 Includes bibliographical references and index Sphingolipids Sphingosine Sphingosine / Laboratory manuals Methods in molecular biology 874 (DE-604)BV035362695 874 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025114209&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Pébay, Alice Sphingosine-1-phosphate methods and protocols Methods in molecular biology Sphingolipids Sphingosine Sphingosine / Laboratory manuals |
| title | Sphingosine-1-phosphate methods and protocols |
| title_auth | Sphingosine-1-phosphate methods and protocols |
| title_exact_search | Sphingosine-1-phosphate methods and protocols |
| title_full | Sphingosine-1-phosphate methods and protocols ed. by Alice Pébay ... |
| title_fullStr | Sphingosine-1-phosphate methods and protocols ed. by Alice Pébay ... |
| title_full_unstemmed | Sphingosine-1-phosphate methods and protocols ed. by Alice Pébay ... |
| title_short | Sphingosine-1-phosphate |
| title_sort | sphingosine 1 phosphate methods and protocols |
| title_sub | methods and protocols |
| topic | Sphingolipids Sphingosine Sphingosine / Laboratory manuals |
| topic_facet | Sphingolipids Sphingosine Sphingosine / Laboratory manuals |
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