Plant salt tolerance: methods and protocols
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Humana Press
2012
|
| Schriftenreihe: | Methods in molecular biology
913 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Klappentext |
| Beschreibung: | XIV, 432 S. Ill., graph. Darst., Kt. |
Internformat
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| 245 | 1 | 0 | |a Plant salt tolerance |b methods and protocols |c Shabala, Sergey ; Tracey Ann Cuin, eds. |
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Datensatz im Suchindex
| _version_ | 1804149245597450240 |
|---|---|
| adam_text | Contents
Preface
.....................................................................................................................
γ
Contributors.
............................................................................................................ xi
Part I Microelectrode Techniques
1
Patch-Clamp Protocols to Study Cell Ionic Homeostasis
Under Saline Conditions
................................................................................... 3
Ana Maria Velarde-Buendta,
René
Alberto Enriquez-Figueroa,
and Igor Pottosin
2
Studying Plant Salt Tolerance with the Voltage Clamp Technique
...................... 19
Zhong-Hua Chen., Dezhi Wu, Cornelia Eisenach, Adrian Hills,
Guoping Zhang, and Michael R.
Blatt
3
Using the Multifunctional Xylem Probe for in situ Studies
of Plant Water and Ion Relations Under Saline Conditions
................................ 35
Lars H.
Wegner
4
Measuring Intracellular Ion Concentrations with
Multi-
Barrelled
Microelectrodes
................................................................................................ 67
Anthony J. Miller and Susan Smith
5
Single-Cell Sampling and Analysis (SiCSA)
........................................................ 79
Wieland Fricke
6
Non
-invasive Flux Measurements Using
Microsensors:
Theory, Limitations, and Systems
...................................................................... 101
Ian Newman, Shao-Liang Chen, D. Marshall Porterfield, and
Jian Sun
7
Quantifying Kinetics of Net Ion Fluxes from Plant Tissues
by Non-invasive Microelectrode Measuring MIFE Technique
............................ 119
Sergey Shabala, Tracey Ann Cuin,
Lana Shabala,
and Ian Newman
Part II Imaging Techniques
8
Quantitative Cryo-Analytical Scanning Electron Microscopy (CEDX):
An Important Technique Useful for Cell-Specific Localization of Salt
................ 137
Margaret McCully and Martin Canny
9
Fluorescence Lifetime Imaging (FLIM) Measurements in Salinity Research
....... 149
Olga Babourina
and Zed Rengel
10
Cytosolic Ca2* Determinations in Studying Plant Responses to Salinity
and Oxidative Stress
.......................................................................................... 163
Antiphon Laohavisit,
Renato
Colaço,
and Julia Davies
11
Infrared Thermography in Plant Phenotyping for Salinity Tolerance
.................. 173
Richard A. James and
Xavier R.R.
Sirault
viii
Contents
12
In vivo Imaging of Nitric Oxide and Reactive Oxygen Species Using
Laser Scanning Confocal Microscopy
................................................................ 191
Yan-Jie Xie and Wen-Biao Shen
Part III Biochemical Assays
13
Metabolomics for Salinity Research
................................................................... 203
Ute
Roessner and Diane M. Beckles
14
Purification of Plant Plasma Membranes by Two-Phase Partitioning
and Measurement of H+ Pumping
..................................................................... 217
Anette
Lund and
Anja Thoe
Fuglsang
15
Determination of Reactive Oxygen Species in Salt-Stressed Plant Tissues
........... 225
Andres Alberto Rodriguez and Edith L. Taleisnik
16
Quantification of the
Antioxidant
Activity in Salt-Stressed Tissues
..................... 237
Jelena
J.
Dragišić Maksimović
and
Branka
D.
Živanović
17
Quantification of Abscisic Acid, Cytokinin, and Auxin Content
in Salt-Stressed Plant Tissues
............................................................................. 251
Petre
I. Dobrev and
Radomíra Vaňkova
Part IV Molkcular Techniques
18
Fluorescence-Activated Cell Sorting for Analysis of Cell
Type-Specific Responses to Salinity Stress in Arabidopsis and Rice
...................... 265
Aurelie
Evrardy Bastiaan O.R.
Bargmann,
Kenneth D.
Birnbaum,
Mark Tester,
Ute Baumann,
and Alexander A.T. Johnson
19
Transformation Using Controlled cDNA Overexpression System
...................... 277
Gábor Rigó, Csaba Papdi,
and
László Szabados
20
Transcriptome Analysis of Membrane Transporters in Response
to Salinity Stress
................................................................................................ 291
Prasad
Senadheera and
Frans J.M. Maathuis
21
Marker-Assisted Selection in Plant Breeding for Salinity Tolerance
..................... 305
M. Ashraf, N.A. Akram, Mehboob-ur-Rahman, and M.R. Footad
22
Transcriptomics on Small Samples
..................................................................... 335
Stuart J. Roy, Simon J. Conn, Gwenda M. Mayo,
Asmini
Athman,
and Matthew Gilliham
23
Plastid Transformation for Abiotic Stress Tolerance in Plants
............................. 351
K.C. Bansal, A.K. Singh, andS.H. Want
24
Manipulating Expression of
Tonoplast
Transporters
.......................................... 359
Zhigang Li, Man Zhou, Qian
Ни,
Shane
Reinhard,
Shuangrong Yuan,
Ning
Yuan, Bekir San, Dayong Li, Haiyan Jia, and Hong Luo
25
Using Heterologous Expression Systems to Characterize Potassium
and Sodium Transport Activities
........................................................................ 371
Alonso
Rodriguez,
Begoña Benito,
and Olivier Cognac
Contents ix
Part V Other Methods
26
Isotope Techniques to Study Kinetics of Na+ and K+ Transport
Under Salinity Conditions
................................................................................. 389
Dev T. Britto and Herbert J.
Ronzucker
27
Trait Dissection of Salinity Tolerance with Plant Phenomics
.............................. 399
Bettina
Berger, Bas
de Regt,
and Mark Tester
28
Measuring Soil Salinity
...................................................................................... 415
Marcus
Hardie
and Richard Doyle
Index
................................................................................................................................ 427
Methods in
Molecular
Biology
913
John M. Walker, Series Editor
Sergey Shabala
·
Tracey Ann Cuin Editors
Plant Salt Tolerance
Methods and Protocols
Soil salinity is destroying several hectares of arable land every minute. Because remedial land
management cannot completely solve the problem, salt tolerant crops or plant species able to re¬
move excessive salt from the soil could contribute significantly to managing the salinity problem.
The key to engineering crops for salt tolerance lies in a thorough understanding of the physi¬
ological mechanisms underlying the adaptive responses of plants to salinity Plant Salt Tolerance:
Methods and Protocols describes recent advances and techniques employed by researchers to
understand the molecular and ionic basis of salinity tolerance and to investigate the mechanisms
of salt stress perception and signalling in plants. With chapters written by leading international
scientists, this book covers nearly
30
different methods, such as microelectrode and molecular
methods, imaging techniques, as well as various biochemical assays. Written in the highly suc¬
cessful Methods in Molecular Biology series format, chapters contain introductions to their
respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible
laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Plant Salt Tolerance: Methods and Protocols serves as an
essential read for every student or researcher tackling various aspects of the
salinit)
problem.
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| spelling | Plant salt tolerance methods and protocols Shabala, Sergey ; Tracey Ann Cuin, eds. New York [u.a.] Humana Press 2012 XIV, 432 S. Ill., graph. Darst., Kt. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 913 Springer protocols Pflanzen (DE-588)4045539-7 gnd rswk-swf Salzresistenz (DE-588)4178992-1 gnd rswk-swf Molekularbiologische Methode (DE-588)7650063-9 gnd rswk-swf Pflanzenzelle (DE-588)4115551-8 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Pflanzenzelle (DE-588)4115551-8 s Salzresistenz (DE-588)4178992-1 s Molekularbiologische Methode (DE-588)7650063-9 s DE-604 Pflanzen (DE-588)4045539-7 s Shabala, Sergey edt Erscheint auch als Online-Ausgabe 978-1-61779-986-0 Methods in molecular biology 913 (DE-604)BV035362695 913 Digitalisierung UB Bayreuth - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025105744&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung UB Bayreuth - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025105744&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Klappentext |
| spellingShingle | Plant salt tolerance methods and protocols Methods in molecular biology Pflanzen (DE-588)4045539-7 gnd Salzresistenz (DE-588)4178992-1 gnd Molekularbiologische Methode (DE-588)7650063-9 gnd Pflanzenzelle (DE-588)4115551-8 gnd |
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| title | Plant salt tolerance methods and protocols |
| title_auth | Plant salt tolerance methods and protocols |
| title_exact_search | Plant salt tolerance methods and protocols |
| title_full | Plant salt tolerance methods and protocols Shabala, Sergey ; Tracey Ann Cuin, eds. |
| title_fullStr | Plant salt tolerance methods and protocols Shabala, Sergey ; Tracey Ann Cuin, eds. |
| title_full_unstemmed | Plant salt tolerance methods and protocols Shabala, Sergey ; Tracey Ann Cuin, eds. |
| title_short | Plant salt tolerance |
| title_sort | plant salt tolerance methods and protocols |
| title_sub | methods and protocols |
| topic | Pflanzen (DE-588)4045539-7 gnd Salzresistenz (DE-588)4178992-1 gnd Molekularbiologische Methode (DE-588)7650063-9 gnd Pflanzenzelle (DE-588)4115551-8 gnd |
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