Verfügbarkeit: Bacterial regulatory RNA

Bacterial regulatory RNA: methods and protocols

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Bibliographische Detailangaben
Format:	Buch
Sprache:	English
Veröffentlicht:	New York, NY [u.a.] Humana Press 2012
Schriftenreihe:	Methods in molecular biology 905
Schlagworte:	Genregulation Molekularbiologische Methode RNS Bakterien Aufsatzsammlung
Online-Zugang:	Inhaltsverzeichnis
Beschreibung:	XI, 333 S. Ill., graph. Darst.

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MARC


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Datensatz im Suchindex

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adam_text	Titel: Bacterial regulatory RNA Autor: Keiler, Kenneth C Jahr: 2012 Contents Preface.......................................................... v Contributors...................................................... ix Part I Identification of Regulatory RNAs 1 Bioinformatic Discovery of Bacterial Regulatory RNAs Using SIPHT....... 3 Jonathan Livny 2 How to Identify CRISPRs in Sequencing Data........................ 15 Christine Drevet and Christine Pourcel 3 A Strategy for Identifying Noncoding RNAs Using Whole-Genome Tiling Arrays..................................... 29 Stephen G. Landt and Eduardo Abeliuk 4 Genetic Screens to Identify Bacterial sRNA Regulators.................. 41 Pierre Mandin Part II Function and Expression of Regulatory RNAs 5 Competition Assays Using Barcoded Deletion Strains to Gain Insight into Small RNA Function.................................. 63 Errett C. Hobbs and Gisela Storz 6 Use of Semi-quantitative Northern Blot Analysis to Determine Relative Quantities of Bacterial CRISPR Transcripts.................... 73 Ksenia Pougach and Konstantin Severinov 7 RNA Visualization in Bacteria by Fluorescence In Situ Hybridization....... 87 Jay H. Russell and Kenneth C. Keiler Part III RNA Structure and Biophysical Measurements 8 RNA Structure Prediction: An Overview of Methods................... 99 Matthew G. Seetin and David H. Mathews 9 Crystallization of RNA-Protein Complexes: From Synthesis and Purification of Individual Components to Crystals.................. 123 Anna Perederina and Andrey S. Krasilnikov 10 Analysis of RNA Folding and Ligand Binding by Conventional and High-Throughput Calorimetry................................. 145 Joshua E. Sokoloski and Philip C. Bevilacqua VII viii Contents Part IV RNA-Protein Interactions 11 Use of Aptamer Tagging to Identify In Vivo Protein Binding Partners of Small Regulatory RNAs................................. 177 Colin P. Corcoran, Renate Rieder, Dimitri Podkaminski, Benjamin Hofmann, and Jörg Vogel 12 Gel Mobility Shift Assays to Detect Protein-RNA Interactions............ 201 Alexander V. Takhnin, Helen Takhnin, and Paul Babitzke 13 RNase Footprinting of Protein Binding Sites on an mRNA Target of Small RNAs........................................... 213 Yi Peng, Toby J. Soper, and Sarah A. Woodson Part V RNA-RNA Interactions 14 Computational Identification of sRNA Targets........................ 227 Brian Tjaden 15 Detection of sRNA-mRNA Interactions by Electrophoretic Mobility Shift Assay............................................ 235 Teppei Morita, Kimika Maki, and Hiroji Aiba 16 Activity of Small RNAs on the Stability of Targeted mRNAs In Vivo........ 245 Guillaume Desnoyers and Eric Massé Part VI Interactions of Regulatory RNA with RNA Polymerase AND THE RlBOSOME 17 Native Gel Electrophoresis to Study the Binding and Release of RNA Polymerase by 6S RNA................................... 259 Karen M. Wassarman 18 Ribosome Purification Approaches for Studying Interactions of Regulatory Proteins and RNAs with the Ribosome................... 273 Preeti Mehta, Perry Woo, Krithika Venkataraman, and A. Wali Karzai 19 Analysis of Aminoacyl- and Peptidyl-tRNAs by Gel Electrophoresis......... 291 Brian D.Janssen, ElieJ. Diner, and Christopher S. Hayes 20 In Vitro Tram-Translation Assays.................................. 311 Daisuke Kurita, Akira Muto, and Hyouta Himeno Index........................................................... 327
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spelling	Bacterial regulatory RNA methods and protocols ed. by Kenneth C. Keiler New York, NY [u.a.] Humana Press 2012 XI, 333 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 905 Genregulation (DE-588)4122166-7 gnd rswk-swf Molekularbiologische Methode (DE-588)7650063-9 gnd rswk-swf RNS (DE-588)4076759-0 gnd rswk-swf Bakterien (DE-588)4004296-0 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Bakterien (DE-588)4004296-0 s RNS (DE-588)4076759-0 s Genregulation (DE-588)4122166-7 s Molekularbiologische Methode (DE-588)7650063-9 s b DE-604 Keiler, Kenneth C. Sonstige oth Methods in molecular biology 905 (DE-604)BV035362695 905 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025105132&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Bacterial regulatory RNA methods and protocols Methods in molecular biology Genregulation (DE-588)4122166-7 gnd Molekularbiologische Methode (DE-588)7650063-9 gnd RNS (DE-588)4076759-0 gnd Bakterien (DE-588)4004296-0 gnd
subject_GND	(DE-588)4122166-7 (DE-588)7650063-9 (DE-588)4076759-0 (DE-588)4004296-0 (DE-588)4143413-4
title	Bacterial regulatory RNA methods and protocols
title_auth	Bacterial regulatory RNA methods and protocols
title_exact_search	Bacterial regulatory RNA methods and protocols
title_full	Bacterial regulatory RNA methods and protocols ed. by Kenneth C. Keiler
title_fullStr	Bacterial regulatory RNA methods and protocols ed. by Kenneth C. Keiler
title_full_unstemmed	Bacterial regulatory RNA methods and protocols ed. by Kenneth C. Keiler
title_short	Bacterial regulatory RNA
title_sort	bacterial regulatory rna methods and protocols
title_sub	methods and protocols
topic	Genregulation (DE-588)4122166-7 gnd Molekularbiologische Methode (DE-588)7650063-9 gnd RNS (DE-588)4076759-0 gnd Bakterien (DE-588)4004296-0 gnd
topic_facet	Genregulation Molekularbiologische Methode RNS Bakterien Aufsatzsammlung
url	http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025105132&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
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