Recombinant and in vitro RNA synthesis: methods and protocols
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Humana Press
2012
|
| Schriftenreihe: | Methods in molecular biology
941 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIV, 274 S. Ill., graph. Darst. |
| ISBN: | 9781627031127 |
Internformat
MARC
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| 245 | 1 | 0 | |a Recombinant and in vitro RNA synthesis |b methods and protocols |c ed. by Graeme L. Conn |
| 264 | 1 | |a New York [u.a.] |b Humana Press |c 2012 | |
| 300 | |a XIV, 274 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 941 | |
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Datensatz im Suchindex
| _version_ | 1804149240899829760 |
|---|---|
| adam_text | Contents
Preface
..........................................................
v
Contributors.
..................................................... xiii
1
Purification of
RNA
Expressed In Vivo Inserted in a tRNA Scaffold
........ 1
Luc Ponchon and
Frédéric
Oardei
2
Selective RNase
H
Cleavage of Target RNAs from a tRNA Scaffold
......... 9
Luc Ponchon,
Geneviève Beauvais, Sylvie Nonin-Lecomte,
and
Frédéric
Oardei
3
Preparation of Long Templates for
RNA
In Vitro Transcription
by Recursive PCR
.............................................. 19
Jessica
С
Bowman, Bahareh Azizi, Timothy K.
Lenz, Poorna
Roy,
and
Loren
Dean Williams
4
General Protocols for Preparation of Plasmid
DNA
Template,
RNA
In Vitro Transcription, and
RNA
Purification by Denaturing PAGE
.... 43
Jo L. Linpinsel and Graeme L. Conn
5
Preparation of Short
RNA
by In Vitro Transcription
.................... 59
Cheng
Lu
and Pingwei Li
6
Native
RNA
Purification by Gel Filtration Chromatography
.............. 69
Evan P. Booy,
Hui Meng,
and Sean A. McKenna
7
Cis-
Acting Ribozymes for the Production of
RNA
In Vitro
Transcripts with Denned
5
and
3
Ends
............................. 83
Johanna M. Avis, Graeme L. Conn, and Scott C. Walker
8
Tnwj-Acting Antigenomic HDV Ribozyme for Production
of In Vitro Transcripts with Homogenous
3
Ends
..................... 99
Milena
Szafraniec,
Leszek
Blaszczyk, Jan Wrzesinski,
and
Jerzy Ciesiołka
9
Rapid Preparation of
RNA
Samples Using
DNA-
Affinity Chromatography
and DNAzyme Methods
......................................... 113
Ног-Кар
Cheong, Eunha Hwang, and Chaejoon Cheong
10
Preparation of
λΝ
-GST
Fusion Protein for Affinity Immobilization of
RNA
. . 123
Geneviève
Di Tomasse, Philipe Lampron, James G. Omicbinski,
and
Pascale
Legault
11
Affinity Purification
of RNA
Using an ARiBo Tag
...................... 137
Geneviève
Di
Tomasso, Pierre Dagenais,
Alexandre Desjardins,
Alexis
Rompre-Brodeur,
Vanessa Delfosse, and
Pascale
Legault
12
Plasmid Template Design and In Vitro Transcription of Short RNAs
Within a Structure Cassette for Structure Probing Experiments
.......... 157
Virginia K. Vacbon and Graeme L· Conn
XI
xii Contents
13 In Vitro Transcription
of Modified RNAs
............................ 171
Stephanie L·
Moon
and Jeffrey Wilusz
14
End-Labeling Oligonucleotides with
Chemical Tags After
Synthesis
........ 181
N. Ruth
Zearfoss and Sean P.
Ryder
15
High-Purity Enzymatic Synthesis of Site-Specifically Modified tRNA
........ 195
Ya
-Міцд
Hou
16
Se-Derivatized RNAs for X-ray Crystallography
........................ 213
Lina Lin
and Zhen Huang
17
Biosynthetk Preparation of 13C/I5N-Labeled rNTPs for High-Resolution
NMRStudies of RNAs
.......................................... 227
Luißi
Martino
and Maria R.
Conte
18
Preparative Separation of Ribonucleoside
Monophosphates
by Ion-Pair Reverse-Phase HPLC
.................................. 247
Pierre Dagenais and
Pascale Legault
19
Splint
Ugarion
of
RNA
with T4
DNA
ligase.........................
257
Christopher
J.
Kershaw and Raymond T. O Keefe
Index
........................................................... 271
|
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| id | DE-604.BV040246727 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T00:19:53Z |
| institution | BVB |
| isbn | 9781627031127 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-025102804 |
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| physical | XIV, 274 S. Ill., graph. Darst. |
| publishDate | 2012 |
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| publisher | Humana Press |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Recombinant and in vitro RNA synthesis methods and protocols ed. by Graeme L. Conn New York [u.a.] Humana Press 2012 XIV, 274 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 941 Springer protocols RNS-Synthese (DE-588)4390988-7 gnd rswk-swf Molekularbiologische Methode (DE-588)7650063-9 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content RNS-Synthese (DE-588)4390988-7 s Molekularbiologische Methode (DE-588)7650063-9 s b DE-604 Conn, Graeme L. edt Methods in molecular biology 941 (DE-604)BV035362695 941 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025102804&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Recombinant and in vitro RNA synthesis methods and protocols Methods in molecular biology RNS-Synthese (DE-588)4390988-7 gnd Molekularbiologische Methode (DE-588)7650063-9 gnd |
| subject_GND | (DE-588)4390988-7 (DE-588)7650063-9 (DE-588)4143413-4 |
| title | Recombinant and in vitro RNA synthesis methods and protocols |
| title_auth | Recombinant and in vitro RNA synthesis methods and protocols |
| title_exact_search | Recombinant and in vitro RNA synthesis methods and protocols |
| title_full | Recombinant and in vitro RNA synthesis methods and protocols ed. by Graeme L. Conn |
| title_fullStr | Recombinant and in vitro RNA synthesis methods and protocols ed. by Graeme L. Conn |
| title_full_unstemmed | Recombinant and in vitro RNA synthesis methods and protocols ed. by Graeme L. Conn |
| title_short | Recombinant and in vitro RNA synthesis |
| title_sort | recombinant and in vitro rna synthesis methods and protocols |
| title_sub | methods and protocols |
| topic | RNS-Synthese (DE-588)4390988-7 gnd Molekularbiologische Methode (DE-588)7650063-9 gnd |
| topic_facet | RNS-Synthese Molekularbiologische Methode Aufsatzsammlung |
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