Persistence of H4N6, H5N1, and H6N8 avian influenza viruses, H1N1 human influenza virus, and two model viruses (NDV and ECBO) in various types of water, lake sediment, duck feces, and meat:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Gießen
VVB Laufersweiler
2011
|
| Ausgabe: | 1. ed. |
| Schriftenreihe: | Edition scientifique
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIV, 160 S. Ill., graph. Darst. 21 cm, 230 g |
| ISBN: | 9783835957428 |
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| 245 | 1 | 0 | |a Persistence of H4N6, H5N1, and H6N8 avian influenza viruses, H1N1 human influenza virus, and two model viruses (NDV and ECBO) in various types of water, lake sediment, duck feces, and meat |c eingereicht von Jawad Nazir. Aus dem Institut für Hygiene und Infektionskrankheiten der Tiere der Justus-Liebig-Universität Gießen und dem Institut für Umwelt- und Tierhygiene der Universität Hohenheim |
| 250 | |a 1. ed. | ||
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| 650 | 7 | |a ducks |2 cabt | |
| 650 | 7 | |a aviary birds |2 cabt | |
| 650 | 7 | |a influenza A |2 cabt | |
| 650 | 7 | |a avian influenza |2 cabt | |
| 650 | 7 | |a microbiology |2 cabt | |
| 650 | 7 | |a faeces |2 cabt | |
| 650 | 7 | |a techniques |2 cabt | |
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Datensatz im Suchindex
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| adam_text | IMAGE 1
TABLE OF CONTENTS I
TABLE OT CONTENTS
TABLE OF CONTENTS I
LIST OF FIGURES VI
LIST OF TABLES IX
ABBREVIATIONS XI
1 INTRODUCTION 1
2 REVIEW OF LITERATURE 3
2.1 INFLUENZA VIRUSES 3
2.1.1 TAXONOMY 3
2.1.2 MORPHOLOGY AND COMPOSITION 3
2.1.3 INFLUENZA A VIRUSES AND THEIR HOST RANGE 4
2.2 AVIAN INFLUENZA 5
2.2.1 LOW AND HIGH PATHOGENICITY AIV 5
2.2.2 ZOONOTIC POTENTIAL OF H5N1 HPAI VIRUSES 6
2.2.3 LABORATORY HOST SYSTEM FOR THE PROPAGATION OF AIV 8
2.2.4 MOLECULAR METHODS FOR THE DETECTION OF AIV 9
2.3 MODES OF TRANSMISSION 9
2.3.1 VIRUS SHEDDING AND TRANSMISSION 9
2.3.2 WATER BORNE TRANSMISSION 10
2.4 PERSISTENCE OF AIV IN AQUATIC HABITATS 10
2.4.1 PERSISTENCE IN WATER 10
2.4.2 EFFECT OF PH AND SALINITY 11
2.4.3 BIOTIC COMPONENTS 13
2.4.4 ABIOTIC RESERVOIRS 14
2.5 TENACITY OF AIV IN SEWAGE, EXCRETA, WASTE, AND POULTRY PRODUCTS 15
2.5.1 INACTIVATION IN SEWAGE 15
2.5.2 PERSISTENCE IN BIRD FECES AND MANURE 16
2.5.3 PERSISTENCE IN POULTRY WASTE AND BYPRODUCTS 17
2.5.4 TENACITY IN BIRD CARCASSES AND MEAT 18
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/1011525291
DIGITALISIERT DURCH
IMAGE 2
TABLE OF CONTENTS II
2.5.5 AIV IN POULTRY EGGS 20
2.6 AIV IN THE ENVIRONMENT 20
2.6.1 ROLE OF THE ENVIRONMENT IN VIRUS TRANSMISSION 20
2.6.2 VIRUS PERSISTENCE ON FOMITES 21
2.6.2.1 SURVIVAL OF AIV ON ENVIRONMENTAL SURFACES 21
2.6.2.2 SURVIVAL OF AIV ON FEATHERS AND DUST PARTICLES 22
2.6.3 PREVALENCE/PERSISTENCE OF AIV IN SEDIMENT 22
2.7 GERM CARRIER TECHNIQUE TO STUDY THE TENACITY OF VIRUSES 23
2.7.1 GERM CARRIER TESTS 23
2.7.2 CARRIER TESTS USED FOR INACTIVATION STUDIES OF INFLUENZA VIRUSES
24
2.7.3 FILTER-BASED CARRIER TESTS 25
3 MATERIALS AND METHODS 27
3.1 VIRUSES AND CELLS 27
3.1.1 VIRUSES 27
3.1.2 CELLSAND CHICKEN EMBRYOS 28
3.2 CHEMICALS, REAGENTS AND SOLUTIONS 28
3.2.1 CELL CULTURE 28
3.2.2 EMBRYO INOCULATION AND HA TEST 31
3.2.3 RRT-PCR 32
3.2.4 TENACITY TRIALS 34
3.3 OTHER MATERIALS AND INSTALMENTS 35
3.3.1 CELL CULTURE AND VIROLOGY 35
3.3.2 RRT-PCR 36
3.3.3 TENACITY TRIALS 36
3.4 VIRUS PROPAGATION 38
3.4.1 PROPAGATION IN SPF CHICKEN EMBRYOS 38
3.4.2 PROPAGATION IN CELL CULTURE 39
3.5 CELL CULTURE PROCEDURE AND PREPARATION OF 96-WELI PLATES 39
3.5.1 MDCK 39
3.5.2 MDBK AND VERO CELLS 39
IMAGE 3
TABLE OF CONTENTS III
3.6 VIRUS TITRATION 40
3.7 BACTERIAL COUNTS 41
3.8 HA TEST PROCEDURE 41
3.9 EXTRACTION OF VIRAL RNA 41
3.10 PCR ANALYSIS 42
3.10.1 RRT-PCR 42
3.10.2 QUANTITATIVE RRT-PCR (QRRT-PCT) 44
3.11 GERM CARRIER TECHNIQUE 44
3.11.1 EFFECT OF LOADING MEDIUM ON VIRUS ADSORPTION TO THE CARRIERS 45
3.11.2 EFFECT OF DRYING ON THE TITER OF FILTER BOUND VIRUS 45
3.12 TENACITY OF THE INFLUENZA AND MODEL VIRUSES IN VARIOUS TYPES OF
WATER.... 46
3.12.1 PERSISTENCE OF THE VIRUSES IN DW AND NS 46
3.12.2 PERSISTENCE OF THE VIRUSES IN SW 46
3.12.2.1 PERSISTENCE OF VIRUSES AFTER SUSPENDING IN THE SW 46
3.12.2.2 PERSISTENCE OF THE VIRUSES IN SW USING GERM CARRIER TECHNIQUE
47
3.13 TENACITY IN LAKE SEDIMENT 48
3.14 TENACITY IN DUCK FECES 50
3.15 TENACITY IN DUCK MEAT 50
3.16 ANALYSIS OF EXPERIMENTAL RESULTS 51
3.16.1 LINEAR REGRESSION ANALYSIS FOR THE SAMPLES STORED AT -10 C 52
3.16.2 CALCULATION OF VIRAL RNA PER ML OF THE SAMPLE 52
4 RESULTS 53
4.1 MICROBIOLOGICAL AND PHYSICOCHEMICAL CHARACTERISTICS OF THE SW USED
IN THE TENACITY TRIALS 53
4.2 TENACITY OF INFLUENZA AND MODEL VIRUSES IN VARIOUS TYPES OF WATER 53
4.2.1 TENACITY OF LPAI H4N6 VIRUS IN DW, NS, AND SW 54
4.2.2 TENACITY OF LPAI H5N1 VIRUS IN DW, NS, AND SW 57
4.2.3 TENACITY OF LPAI H6N8 VIRUS IN DW, NS, AND SW 60
4.2.4 TENACITY OF HUMAN INFLUENZA VIRUS H1N1 IN DW, NS, AND SW 63
4.2.5 TENACITY OF NDV IN DW, NS, AND SW 66
IMAGE 4
TABLE OF CONTENTS IV
4.2.6 TENACITY OF ECBO IN DW, NS, AND SW 69
4.3 COMPARISON OF T-90 VALUES OF THE VIRUSES IN WATER 72
4.4 PERSISTENCE OF VIRAL RNA IN THE INOCULATED WATER SAMPLES 75
4.5 SUITABILITY OF THE GERM CARRIER TECHNIQUE 78
4.6 TENACITY OF THE INFLUENZA AND MODEL VIRUSES IN VARIOUS SUBSTRATES
USING THE GERM CARRIER TECHNIQUE 81
4.6.1 TENACITY OF LPAI H4N6 VIRUS IN SW, SEDIMENT, DUCK FECES, AND MEAT
AT VARIOUS TEMPERATURES 81
4.6.2 TENACITY OF LPAI H5N1 VIRUS IN SW, SEDIMENT, DUCK FECES, AND MEAT
AT VARIOUS TEMPERATURES 85
4.6.3 TENACITY OF LPAI H6N8 VIRUS IN SW, SEDIMENT, DUCK FECES, AND MEAT
AT VARIOUS TEMPERATURES 88
4.6.4 TENACITY OF HUMAN INFLUENZA VIRUS H1N1 IN SW, SEDIMENT, DUCK
FECES, AND MEAT AT VARIOUS TEMPERATURES 91
4.6.5 TENACITY OF NDV IN SW, SEDIMENT, DUCK FECES, AND MEAT AT VARIOUS
TEMPERATURES 94
4.6.6 TENACITY OF ECBO IN SW, SEDIMENT, DUCK FECES, AND MEAT AT VARIOUS
TEMPERATURES 97
4.7 COMPARISON OF T-90 VALUES OF THE VIRUSES IN VARIOUS SUBSTRATES AS
CALCULATED USING THE GERM CARRIER TECHNIQUE 100
4.8 COMPARISON OF T-90 VALUES OF THE INFLUENZA AND MODEL VIRUSES
CALCULATED BY SUSPENDING THE VIRUS IN WATER AND USING THE GERM CARRIER
TECHNIQUE 103
5 DISCUSSION 110
5.1 TENACITY OF INFLUENZA AND MODEL VIRUSES IN WATER 110
5.2 PERSISTENCE OF VIRAL RNA IN THE AIV INOCULATED SW 113
5.3 APPROPRIATENESS OF THE GERM CARRIER TECHNIQUE 114
5.4 COMPARATIVE PERSISTENCE OF THE VIRUSES IN SW BY DIRECT SUSPENSION
AND USING THE GERM CARRIER TECHNIQUE 117
5.5 PERSISTENCE OF THE INFLUENZA AND MODEL VIRUSES IN LAKE SEDIMENT 118
5.6 PERSISTENCE OF INFLUENZA AND MODEL VIRUSES IN DUCK FECES AND FECAL
WASTE 120
IMAGE 5
TABLE OF CONTENTS V
5.7 PERSISTENCE OF THE INFLUENZA AND MODEL VIRUSES IN MEAT AND BIRD
CARCASSES 122
5.8 SIGNIFICANCE OF MODEL VIRUSES INCLUDED IN THE STUDY 124
5.9 CONCLUSIONS 126
6 SUMMARY 127
7 ZUSAMMENFASSUNG 130
8 LITERATURE CITED 134
9 APPENDIX 148
10 ACKNOWLEDGEMENTS 158
|
| any_adam_object | 1 |
| author | Nazir, Jawad |
| author_GND | (DE-588)141224940 |
| author_facet | Nazir, Jawad |
| author_role | aut |
| author_sort | Nazir, Jawad |
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| bvnumber | BV040239638 |
| ctrlnum | (OCoLC)725275514 (DE-599)DNB1011525291 |
| dewey-full | 571.9118176 571.98629256165 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects |
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| dewey-tens | 570 - Biology |
| discipline | Biologie |
| edition | 1. ed. |
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| isbn | 9783835957428 |
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| spelling | Nazir, Jawad Verfasser (DE-588)141224940 aut Persistence of H4N6, H5N1, and H6N8 avian influenza viruses, H1N1 human influenza virus, and two model viruses (NDV and ECBO) in various types of water, lake sediment, duck feces, and meat eingereicht von Jawad Nazir. Aus dem Institut für Hygiene und Infektionskrankheiten der Tiere der Justus-Liebig-Universität Gießen und dem Institut für Umwelt- und Tierhygiene der Universität Hohenheim 1. ed. Gießen VVB Laufersweiler 2011 XIV, 160 S. Ill., graph. Darst. 21 cm, 230 g txt rdacontent n rdamedia nc rdacarrier Edition scientifique Zugl.: Gießen, Univ., Diss., 2011 viruses cabt ducks cabt aviary birds cabt influenza A cabt avian influenza cabt microbiology cabt faeces cabt techniques cabt Tenazität (DE-588)4297524-4 gnd rswk-swf Wasservögel (DE-588)4064815-1 gnd rswk-swf Persistenz (DE-588)4136610-4 gnd rswk-swf Influenzaviren (DE-588)4254831-7 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Wasservögel (DE-588)4064815-1 s Influenzaviren (DE-588)4254831-7 s Tenazität (DE-588)4297524-4 s Persistenz (DE-588)4136610-4 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025095860&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Nazir, Jawad Persistence of H4N6, H5N1, and H6N8 avian influenza viruses, H1N1 human influenza virus, and two model viruses (NDV and ECBO) in various types of water, lake sediment, duck feces, and meat viruses cabt ducks cabt aviary birds cabt influenza A cabt avian influenza cabt microbiology cabt faeces cabt techniques cabt Tenazität (DE-588)4297524-4 gnd Wasservögel (DE-588)4064815-1 gnd Persistenz (DE-588)4136610-4 gnd Influenzaviren (DE-588)4254831-7 gnd |
| subject_GND | (DE-588)4297524-4 (DE-588)4064815-1 (DE-588)4136610-4 (DE-588)4254831-7 (DE-588)4113937-9 |
| title | Persistence of H4N6, H5N1, and H6N8 avian influenza viruses, H1N1 human influenza virus, and two model viruses (NDV and ECBO) in various types of water, lake sediment, duck feces, and meat |
| title_auth | Persistence of H4N6, H5N1, and H6N8 avian influenza viruses, H1N1 human influenza virus, and two model viruses (NDV and ECBO) in various types of water, lake sediment, duck feces, and meat |
| title_exact_search | Persistence of H4N6, H5N1, and H6N8 avian influenza viruses, H1N1 human influenza virus, and two model viruses (NDV and ECBO) in various types of water, lake sediment, duck feces, and meat |
| title_full | Persistence of H4N6, H5N1, and H6N8 avian influenza viruses, H1N1 human influenza virus, and two model viruses (NDV and ECBO) in various types of water, lake sediment, duck feces, and meat eingereicht von Jawad Nazir. Aus dem Institut für Hygiene und Infektionskrankheiten der Tiere der Justus-Liebig-Universität Gießen und dem Institut für Umwelt- und Tierhygiene der Universität Hohenheim |
| title_fullStr | Persistence of H4N6, H5N1, and H6N8 avian influenza viruses, H1N1 human influenza virus, and two model viruses (NDV and ECBO) in various types of water, lake sediment, duck feces, and meat eingereicht von Jawad Nazir. Aus dem Institut für Hygiene und Infektionskrankheiten der Tiere der Justus-Liebig-Universität Gießen und dem Institut für Umwelt- und Tierhygiene der Universität Hohenheim |
| title_full_unstemmed | Persistence of H4N6, H5N1, and H6N8 avian influenza viruses, H1N1 human influenza virus, and two model viruses (NDV and ECBO) in various types of water, lake sediment, duck feces, and meat eingereicht von Jawad Nazir. Aus dem Institut für Hygiene und Infektionskrankheiten der Tiere der Justus-Liebig-Universität Gießen und dem Institut für Umwelt- und Tierhygiene der Universität Hohenheim |
| title_short | Persistence of H4N6, H5N1, and H6N8 avian influenza viruses, H1N1 human influenza virus, and two model viruses (NDV and ECBO) in various types of water, lake sediment, duck feces, and meat |
| title_sort | persistence of h4n6 h5n1 and h6n8 avian influenza viruses h1n1 human influenza virus and two model viruses ndv and ecbo in various types of water lake sediment duck feces and meat |
| topic | viruses cabt ducks cabt aviary birds cabt influenza A cabt avian influenza cabt microbiology cabt faeces cabt techniques cabt Tenazität (DE-588)4297524-4 gnd Wasservögel (DE-588)4064815-1 gnd Persistenz (DE-588)4136610-4 gnd Influenzaviren (DE-588)4254831-7 gnd |
| topic_facet | viruses ducks aviary birds influenza A avian influenza microbiology faeces techniques Tenazität Wasservögel Persistenz Influenzaviren Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025095860&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT nazirjawad persistenceofh4n6h5n1andh6n8avianinfluenzavirusesh1n1humaninfluenzavirusandtwomodelvirusesndvandecboinvarioustypesofwaterlakesedimentduckfecesandmeat |