Leukocytic acetylcholine in chronic rejection of renal allografts:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Giessen
VVB Laufersweiler
2011
|
| Ausgabe: | 1. Aufl. |
| Schriftenreihe: | Edition scientifique
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | VIII, 79 S. Ill., graph. Darst. |
| ISBN: | 9783835958166 |
Internformat
MARC
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| 100 | 1 | |a Wilczynska, Joanna |d 1983- |e Verfasser |0 (DE-588)1016469853 |4 aut | |
| 245 | 1 | 0 | |a Leukocytic acetylcholine in chronic rejection of renal allografts |c by Joanna Wilczynska |
| 250 | |a 1. Aufl. | ||
| 264 | 1 | |a Giessen |b VVB Laufersweiler |c 2011 | |
| 300 | |a VIII, 79 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 0 | |a Edition scientifique | |
| 502 | |a Zugl.: Gießen, Univ., Diss., 2011 | ||
| 650 | 7 | |a acetylcholine |2 cabt | |
| 650 | 7 | |a neurotransmitters |2 cabt | |
| 650 | 7 | |a parasympathomimetics |2 cabt | |
| 650 | 7 | |a acetylcholinesterase |2 cabt | |
| 650 | 7 | |a leukocytes |2 cabt | |
| 650 | 7 | |a allografts |2 cabt | |
| 650 | 7 | |a kidneys |2 cabt | |
| 650 | 7 | |a blood vessels |2 cabt | |
| 650 | 7 | |a polymerase chain reaction |2 cabt | |
| 650 | 7 | |a glomerulus |2 cabt | |
| 650 | 0 | 7 | |a Nierentransplantation |0 (DE-588)4042284-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Acetylcholin |0 (DE-588)4141256-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Allogenes Transplantat |0 (DE-588)4141972-8 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Tiermodell |0 (DE-588)4140660-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Gefäßkrankheit |0 (DE-588)4071631-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Pathogenese |0 (DE-588)4115512-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Leukozyt |0 (DE-588)4074195-3 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Nierentransplantation |0 (DE-588)4042284-7 |D s |
| 689 | 0 | 1 | |a Allogenes Transplantat |0 (DE-588)4141972-8 |D s |
| 689 | 0 | 2 | |a Gefäßkrankheit |0 (DE-588)4071631-4 |D s |
| 689 | 0 | 3 | |a Pathogenese |0 (DE-588)4115512-9 |D s |
| 689 | 0 | 4 | |a Leukozyt |0 (DE-588)4074195-3 |D s |
| 689 | 0 | 5 | |a Acetylcholin |0 (DE-588)4141256-4 |D s |
| 689 | 0 | 6 | |a Tiermodell |0 (DE-588)4140660-6 |D s |
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Datensatz im Suchindex
| _version_ | 1804149228525584384 |
|---|---|
| adam_text | IMAGE 1
TABLES OF CONTENT
I TABLES OF CONTENT
1. INTRODUCTION 1
1.1 KIDNEY TRANSPLANTATION - GRAFT LOSS AND PATIENT SURVIVAL 1
1.2 ALLOGRAFT REJECTION 2
1.2.1 CHRONIC ALLOGRAFT VASCULOPATHY (CAV) 3
1.2.2 TRANSPLANT GLOMERULOPATHY (TG) 4
1.2.3 TUBULAR ATROPHY (TA) AND INTERSTITIAL FIBROSIS (IF) 5
1.3 EXPERIMENTAL MODELS FOR CHRONIC RENAL ALLOGRAFT REJECTION 5
1.4 FROM ACETYLCHOLINE (ACH) TO CHRONIC ALLOGRAFT VASCULOPATHY 6
1.4.1 ACH IN FATAL ACUTE REJECTION 6
1.4.2 ACH IN VASCULAR REMODELLING 7
1.4.3 ACH AND NICOTINE - LIGANDS FORNICOTINIC ACH RECEPTORS 7
1.4.4 ACH SYNTHESIS IN NON-NEURONAL CELLS 8
1.4.4.1 CHOLINE ACETYLTRANSFERASE (CHAT) GENE AND PROTEIN 9
1.4.4.1.1 CARNITINE ACETYLTRANSFERASE (CARAT) 11
1.4.4.2 HIGH AFFINITY CHOLINE TRANSPORTER (CHT1) AND CHOLINE TRANSPORT
11
1.4.5 ACH TRANSPORT AND RELEASE - VASCULAR ACETYLCHOLINE TRANSPORTER
(VACHT) AND
ORGANIC CATION TRANSPORTERS (OCTS) 12
1.4.6 ACH DEGRADATION - ACETYLCHOLINESTERASE
(ACHE)/BUTYRYLCHOLINESTERASE
(BCHE) 12
1.4.7 ACHE/BCHE INHIBITOR - RIVASTIGMINE 13
2. HYPOTHESES 14
3. MATERIALS AND METHODS 15
3.1 MATERIALS 15
3.1.1 REAGENTS AND CONSUMABLES 15
3.1.2 EQUIPMENT 18
3.1.3 BUFFERS AND SOLUTIONS 19
3.2 METHODS 24
3.2.1 TRANSPLANTATION 24
3.2.1.1 EXPERIMENTAL ANIMALS 24
3.2.1.2 RENAL TRANSPLANTATION AND TREATMENT WITH RIVASTIGMINE 24
3.2.1.3 RENAL FUNCTION 24
I
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/1014795737
DIGITALISIERT DURCH
IMAGE 2
TABLES OF CONTENT
3.2.1.4 PERFUSION OF THE RENAL VASCULATURE 25
3.2.1.5 PURIFICATION OF LEUKOCYTES 25
3.2.1.6 ORGAN FIXATION AND EMBEDDING 25
3.2.2 HISTOLOGY AND IMMUNOHISTOCHEMISTRY 26
3.2.2.1 SILANISING GLASS SLIDES 26
3.2.2.2 PREPARATION OF SECTIONS, DEWAXING AND REHYDRATION 26
3.2.2.3 HISTOLOGY 26
3.2.2.3.1 TAENZER-UNNA ACIDIC ORCEIN STAINING 26
3.2.2.3.2 HEIDENHEIN AZAN STAINING 27
3.2.2.3.3 GOMORI METHENAMINE SILVER STAINING 27
3.2.2.4 IMMUNOHISTOCHEMISTRY 27
3.2.2.4.1 SINGLE-STAINING 27
3.2.2.4.2 PEPTIDE COMPETITION ASSAY (PCA) 29
3.2.2.4.3 DOUBLE-STAINING 29
3.2.3 PROTEIN BIOCHEMISTRY 29
3.2.3.1 PROTEIN ASSAY AND SAMPLE PREPARATION 29
3.2.3.2 SDS-POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE) 30
3.2.3.3 TRANSFER 30
3.2.3.4 BLOCKING AND DETECTION OF ANTIGENS 31
3.2.3.5 DENSITOMETRICAL EVALUATION AND STATISTICS 31
3.2.4 DETECTION OF DNA 32
3.2.4.1 RNA ISOLATION 32
3.2.4.2 SYNTHESIS OF COMPLEMENTARY DNA (CDNA) 32
3.2.4.3 REAL-TIME QUANTITATIVE PCR (QPCR) 32
3.2.4.3.1 AGAROSEGEL ELECTROPHORESIS 33
3.2.4.3.2 DNA SEQUENCING 34
4. RESULTS 35
4.1 HISTOPATHOLOGY OF KIDNEYS TRANSPLANTED IN THE F344 TO LEW RAT STRAIN
COMBINATION 35
4.2 NUMBER OF INTRAVASCULAR GRAFT LEUKOCYTES 39
4.3 EXPRESSION OF CHT1, CHAT AND CARAT MRNA BY GRAFT BLOOD LEUKOCYTES 41
4.4 EXPRESSION OF CHT1 AND CHAT MRNA IN RENAL TISSUE 43
4.5 EXPRESSION OF CHT1 AND CHAT PROTEIN BY GRAFT BLOOD LEUKOCYTES 45
4.6 EXPRESSION OF CHT1 AND CHAT PROTEIN BY GRAFTS BLOOD MONOCYTES 45
N
IMAGE 3
TABLES OF CONTENT
4.7 ACH CONCENTRATION IN GRAFT BLOOD LEUKOCYTES 50
4.8 VACHT, OCT1, OCT2 AND OCT3 MRNA-EXPRESSION BY GRAFT BLOOD
LEUKOCYTES 50
4.9 RIVASTIGMINE-TREATMENTOFRENAL ALLOGRAFT RECIPIENTS 51
5. DISCUSSION 56
5.1 EXPERIMENTAL RENAL TRANSPLANTATION 56
5.2 LEUKOCYTE ACCUMULATION 57
5.3 THICKNESS OF ARTERIAL WALL DURING REVERSIBLE ACUTE REJECTION 57
5.4 CAN 58
5.5 ACH SYNTHESIS 58
5.6 LEUKOCYTIC ACH CONTENT 59
5.7 ACH RELEASE 60
5.8 ACH AND CAV 61
5.9 CONCLUSIONS 62
6. SUMMARY 63
7. ZUSAMMENFASSUNG 64
8. LITERATURE 65
9. DECLARATION 76
10. CURRICULUM VITAE 77
11. ACKNOWLEDGEMENTS 79
|
| any_adam_object | 1 |
| author | Wilczynska, Joanna 1983- |
| author_GND | (DE-588)1016469853 |
| author_facet | Wilczynska, Joanna 1983- |
| author_role | aut |
| author_sort | Wilczynska, Joanna 1983- |
| author_variant | j w jw |
| building | Verbundindex |
| bvnumber | BV040238097 |
| ctrlnum | (OCoLC)758479898 (DE-599)DNB1014795737 |
| dewey-full | 617.4610592 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 617 - Surgery & related medical specialties |
| dewey-raw | 617.4610592 |
| dewey-search | 617.4610592 |
| dewey-sort | 3617.4610592 |
| dewey-tens | 610 - Medicine and health |
| discipline | Medizin |
| edition | 1. Aufl. |
| format | Thesis Book |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV040238097 |
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| indexdate | 2024-07-10T00:19:42Z |
| institution | BVB |
| isbn | 9783835958166 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-025094343 |
| oclc_num | 758479898 |
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| owner_facet | DE-188 |
| physical | VIII, 79 S. Ill., graph. Darst. |
| publishDate | 2011 |
| publishDateSearch | 2011 |
| publishDateSort | 2011 |
| publisher | VVB Laufersweiler |
| record_format | marc |
| series2 | Edition scientifique |
| spelling | Wilczynska, Joanna 1983- Verfasser (DE-588)1016469853 aut Leukocytic acetylcholine in chronic rejection of renal allografts by Joanna Wilczynska 1. Aufl. Giessen VVB Laufersweiler 2011 VIII, 79 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Edition scientifique Zugl.: Gießen, Univ., Diss., 2011 acetylcholine cabt neurotransmitters cabt parasympathomimetics cabt acetylcholinesterase cabt leukocytes cabt allografts cabt kidneys cabt blood vessels cabt polymerase chain reaction cabt glomerulus cabt Nierentransplantation (DE-588)4042284-7 gnd rswk-swf Acetylcholin (DE-588)4141256-4 gnd rswk-swf Allogenes Transplantat (DE-588)4141972-8 gnd rswk-swf Tiermodell (DE-588)4140660-6 gnd rswk-swf Gefäßkrankheit (DE-588)4071631-4 gnd rswk-swf Pathogenese (DE-588)4115512-9 gnd rswk-swf Leukozyt (DE-588)4074195-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Nierentransplantation (DE-588)4042284-7 s Allogenes Transplantat (DE-588)4141972-8 s Gefäßkrankheit (DE-588)4071631-4 s Pathogenese (DE-588)4115512-9 s Leukozyt (DE-588)4074195-3 s Acetylcholin (DE-588)4141256-4 s Tiermodell (DE-588)4140660-6 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025094343&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Wilczynska, Joanna 1983- Leukocytic acetylcholine in chronic rejection of renal allografts acetylcholine cabt neurotransmitters cabt parasympathomimetics cabt acetylcholinesterase cabt leukocytes cabt allografts cabt kidneys cabt blood vessels cabt polymerase chain reaction cabt glomerulus cabt Nierentransplantation (DE-588)4042284-7 gnd Acetylcholin (DE-588)4141256-4 gnd Allogenes Transplantat (DE-588)4141972-8 gnd Tiermodell (DE-588)4140660-6 gnd Gefäßkrankheit (DE-588)4071631-4 gnd Pathogenese (DE-588)4115512-9 gnd Leukozyt (DE-588)4074195-3 gnd |
| subject_GND | (DE-588)4042284-7 (DE-588)4141256-4 (DE-588)4141972-8 (DE-588)4140660-6 (DE-588)4071631-4 (DE-588)4115512-9 (DE-588)4074195-3 (DE-588)4113937-9 |
| title | Leukocytic acetylcholine in chronic rejection of renal allografts |
| title_auth | Leukocytic acetylcholine in chronic rejection of renal allografts |
| title_exact_search | Leukocytic acetylcholine in chronic rejection of renal allografts |
| title_full | Leukocytic acetylcholine in chronic rejection of renal allografts by Joanna Wilczynska |
| title_fullStr | Leukocytic acetylcholine in chronic rejection of renal allografts by Joanna Wilczynska |
| title_full_unstemmed | Leukocytic acetylcholine in chronic rejection of renal allografts by Joanna Wilczynska |
| title_short | Leukocytic acetylcholine in chronic rejection of renal allografts |
| title_sort | leukocytic acetylcholine in chronic rejection of renal allografts |
| topic | acetylcholine cabt neurotransmitters cabt parasympathomimetics cabt acetylcholinesterase cabt leukocytes cabt allografts cabt kidneys cabt blood vessels cabt polymerase chain reaction cabt glomerulus cabt Nierentransplantation (DE-588)4042284-7 gnd Acetylcholin (DE-588)4141256-4 gnd Allogenes Transplantat (DE-588)4141972-8 gnd Tiermodell (DE-588)4140660-6 gnd Gefäßkrankheit (DE-588)4071631-4 gnd Pathogenese (DE-588)4115512-9 gnd Leukozyt (DE-588)4074195-3 gnd |
| topic_facet | acetylcholine neurotransmitters parasympathomimetics acetylcholinesterase leukocytes allografts kidneys blood vessels polymerase chain reaction glomerulus Nierentransplantation Acetylcholin Allogenes Transplantat Tiermodell Gefäßkrankheit Pathogenese Leukozyt Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025094343&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT wilczynskajoanna leukocyticacetylcholineinchronicrejectionofrenalallografts |