Tetrahymena thermophila:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2012
|
| Ausgabe: | 1. ed. |
| Schriftenreihe: | Methods in cell biology
109 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIII, 452 S., [4] Bl. Ill., graph. Darst. |
| ISBN: | 9780123859679 |
Internformat
MARC
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|---|---|---|---|
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| 003 | DE-604 | ||
| 005 | 20120611 | ||
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| 008 | 120604s2012 ad|| |||| 00||| eng d | ||
| 020 | |a 9780123859679 |9 978-0-12-385967-9 | ||
| 035 | |a (OCoLC)796265409 | ||
| 035 | |a (DE-599)BVBBV040234213 | ||
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| 245 | 1 | 0 | |a Tetrahymena thermophila |c ed. by Kathleen Collins |
| 250 | |a 1. ed. | ||
| 264 | 1 | |a Amsterdam [u.a.] |b Elsevier, Acad. Press |c 2012 | |
| 300 | |a XIII, 452 S., [4] Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in cell biology |v 109 | |
| 650 | 0 | 7 | |a Cytologie |0 (DE-588)4070177-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Tetrahymena thermophila |0 (DE-588)4274357-6 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
| 689 | 0 | 0 | |a Tetrahymena thermophila |0 (DE-588)4274357-6 |D s |
| 689 | 0 | 1 | |a Cytologie |0 (DE-588)4070177-3 |D s |
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| 700 | 1 | |a Collins, Kathleen |e Sonstige |0 (DE-588)1022649698 |4 oth | |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-025090555 | ||
Datensatz im Suchindex
| _version_ | 1804149223102349312 |
|---|---|
| adam_text | CONTENTS
Contributors
xi
Preface
xiii
PART I
—
Introduction
1.
Perspectives on the Ciliated Protozoan Tetrahymena thermophila
Kathleen Collins
I. Introduction
4
II. Historical Contributions
4
III. Compelling Opportunities
5
IV Chapter Logic
6
Acknowledgments
6
References
6
PART II
—
Systems Perspectives
2.
The Life and Times of Tetrahymena
Denis H. Lynn and F. Paul Doerder
I. Introduction
12
II. Brief History
12
III. Description
13
IV. Life Cycles and Breeding Systems
22
V Evolution of Tetrahymena
23
VI. Perspective
24
References
25
3.
Nuclear Dualism
Kathleen M. Karrer
I. Vegetative Cell Division
30
II. Sexual Reproduction
31
HI. Chromatin Structure
32
IV Molecular Events of MAC
Anlagen
Development
35
V. Nuclear Transport
44
References
45
Contents
4.
Whole
Genome
Studies of Tetrahymena
Robert S. Coyne, Nicholas
A. Stoverand
Wei
Miao
I. Introduction
54
II. To Make a Long Story Short: A Brief History of the
Tetrahymena MAC Genome Project
54
III. Examples of Use
55
IV. Near-Term Goals for Improvement of Genomic Resources
59
V Looking Further Ahead
68
VI. Concluding Remarks
74
Acknowledgments
75
References
75
5.
From Molecules to Morphology: Cellular Organization
of Tetrahymena thermophila
Dorota Wloga
and Joseph
Frankéi
I. Overview
84
II. Basal Bodies
86
III. Cilia
90
IV. Cortical Microtubular Arrays and Non-microtubular Fibers
96
V Epiplasm (Membrane Skeleton)
99
VI. Apical Crown, Apical Band, and Contractile Ring
101
VII.
Oral Apparatus
103
VFIÍ.
Cytoproct
108
IX. Contractile
Vacuole
109
X. Mitochondria
110
XI. Nuclear Structure and Nuclear-Cortical Interaction
115
XII.
Identification of Genes Involved in Cortical Organization
120
Acknowledgments
123
References
124
6.
Conservation and Innovation in Tetrahymena Membrane Traffic:
Proteins, Lipids, and Compartments
Alejandro D. Nusblat,
Lydia
J. Bright, and Aaron P. Turkewitz
I. Introduction
142
II. Recent Work on Membrane Traffic in Tetrahymena
146
III. Studieson
Membrane Lipids in Tetrahymena
154
IV. Conservation Versus Innovation
161
V. Using Expression Data to Elucidate Pathways
of Membrane Traffic
165
Acknowledgments
169
References
169
Contents
7.
Developmental progression of Tetrahymena through the cell cycle and conjugation
Eric Cole and Toshiro
Sugai
I. Introduction
178
II. Checkpoints and Developmental Contingencies
179
III. Vegetative Development (Asexual Reproduction by Cellular Fission)
180
IV. Alternatives to Cell Division
198
Y
Conjugal Development
198
VI. Developmental Disruptions
204
VII.
Overview of the Developmental Logic of Conjugation
225
Acknowledgments
229
References
229
PART III
—
Operating Principles
8.
Tetrahymena in the Laboratory: Strain Resources, Methods for Culture,
Maintenance, and Storage
Donna M. Cassidy-Hartley
1.
Introduction
240
II. Tetrahymena thermophila Strains
240
II I. Other Tetrahymena Species
245
IV. Cell Culture Media
246
V. Cell Culture
256
VI.
Long-Term
Storage
267
Acknowledgments
270
References
270
9.
Natural Populations and Inbred Strains of Tetrahymena
E Paul
Doerder and Clifford Brunk
I. Introduction/Background
278
II. The Species Problem
279
III. Tetrahymena Collections
280
IV Species of Tetrahymena
283
V Methods for Collecting and Identification of Tetrahymena
287
References
298
10.
Tetrahymena thermophila Genetics: Concepts and Applications
Eduardo
Orias
I. Introduction
302
II. Fundamental Concepts of Tetrahymena Genetics
302
III. Basic Genetic Operations
308
IV Genetic Mapping
315
V Isolating and Using Mutants: identifying the Mutant Gene
318
Acknowledgments
323
References
323
Contents
11. Transformation
and Strain
Engineering
of
Tetrahymena
Douglas L. Chalker
I. Introduction
328
II. Strategies for Manipulating the Genome of Tetrahymena Strains
329
III. Generating Gene Knockouts
330
IV Engineering Strains for Expression of Tagged
Alíeles
333
V. Selectable Markers and Expression Vectors
336
VI. The Use of Heterokaryon Strains for the Study of Essential Genes
337
VII.
Executing Biolistics Transformation and Electroporation
337
VIII.
Summary
343
Acknowledgments
343
References
344
12.
Biochemical Approaches Including the Design and Use of Strains Expressing
Epitope-Tagged Proteins
Mary T. Couvillion and Kathleen Collins
I. Introduction: General Considerations for Strain Construction
348
H. Whole-Cell Protein and Nucleic Acid Isolation,
Detection, and Quantification
350
III. Cell
Lysáte
Preparation and Affinity Purification
352
IV. Detection of Recovered Complexes
353
Acknowledgments
354
References
354
13.
Cytological Analysis of Tetrahymena thermophila
Mark Winey,
Alexander!
Stemm-Wolf, Thomas
H Giddings
Jr.,
and Chad G. Pearson
I. Introduction
358
II. Rationale
358
III. Light Microscopy
359
IV Electron Microscopy
368
V. Discussion
375
Acknowledgments
376
References
376
14.
Purification of Tetrahymena Cytoskeletal Proteins
Jerry E. Honts
I. Overview
379
II. Purification of Actin and Actin-Binding Proteins
380
III. Purification of Tubulin and Tubulin-Associated Proteins
382
IV. Purification of Proteins from the Cortical Cytoskeleton
382
V. Challenges and Opportunities
388
Acknowledgments
389
References
389
Contents
15.
Behavioral
Bioassays
and Their Uses in Tetrahymena
Todd M. Hennessey and Thomas J. Lampert
I. Introduction
393
II. Materials and Methods
396
III. Discussion and Summary
406
References
407
16.
Tetrahymena in the Classroom
Joshua J. Smith, Emily A. Wiley, and Donna M. Cassidy-Hanley
I. Introduction/Rationale
412
II. Materials and Methods
414
III. Discussion
427
Acknowledgments
429
References
429
Index
431
Volumes in Series
443
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| illustrated | Illustrated |
| indexdate | 2024-07-10T00:19:37Z |
| institution | BVB |
| isbn | 9780123859679 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-025090555 |
| oclc_num | 796265409 |
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| physical | XIII, 452 S., [4] Bl. Ill., graph. Darst. |
| publishDate | 2012 |
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| series | Methods in cell biology |
| series2 | Methods in cell biology |
| spelling | Tetrahymena thermophila ed. by Kathleen Collins 1. ed. Amsterdam [u.a.] Elsevier, Acad. Press 2012 XIII, 452 S., [4] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in cell biology 109 Cytologie (DE-588)4070177-3 gnd rswk-swf Tetrahymena thermophila (DE-588)4274357-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Tetrahymena thermophila (DE-588)4274357-6 s Cytologie (DE-588)4070177-3 s DE-604 Collins, Kathleen Sonstige (DE-588)1022649698 oth Methods in cell biology 109 (DE-604)BV002534878 109 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025090555&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Tetrahymena thermophila Methods in cell biology Cytologie (DE-588)4070177-3 gnd Tetrahymena thermophila (DE-588)4274357-6 gnd |
| subject_GND | (DE-588)4070177-3 (DE-588)4274357-6 (DE-588)4143413-4 |
| title | Tetrahymena thermophila |
| title_auth | Tetrahymena thermophila |
| title_exact_search | Tetrahymena thermophila |
| title_full | Tetrahymena thermophila ed. by Kathleen Collins |
| title_fullStr | Tetrahymena thermophila ed. by Kathleen Collins |
| title_full_unstemmed | Tetrahymena thermophila ed. by Kathleen Collins |
| title_short | Tetrahymena thermophila |
| title_sort | tetrahymena thermophila |
| topic | Cytologie (DE-588)4070177-3 gnd Tetrahymena thermophila (DE-588)4274357-6 gnd |
| topic_facet | Cytologie Tetrahymena thermophila Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025090555&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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