Amino acid absorption and homeostasis in mice lacking the intestinal peptide transporter PEPT1:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online-Zugang: | kostenfrei https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20120508-1081579-1-0 Inhaltsverzeichnis |
| Beschreibung: | Zsfassung in dt. Sprache |
| Beschreibung: | 125 S. Ill., graph. Darst. |
Internformat
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| 100 | 1 | |a Näßl, Anna-Maria |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Amino acid absorption and homeostasis in mice lacking the intestinal peptide transporter PEPT1 |c Anna-Maria Näßl |
| 264 | 1 | |c 2012 | |
| 300 | |a 125 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
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| 500 | |a Zsfassung in dt. Sprache | ||
| 502 | |a München, Techn. Univ., Diss., 2012 | ||
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Datensatz im Suchindex
| _version_ | 1804149220930748416 |
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| adam_text | IMAGE 1
TABLE OF CONTENT
SUMMARY 13
ZUSAMMENFASSUNG 15
1. INTRODUCTION 17
1.1. PEPTIDE TRANSPORT AND PEPTIDE TRANSPORTERS - A BRIEF HISTORY 17
1.2. THE PEPTIDE TRANSPORTER PEPT1 18
1.2.1. MODE OF TRANSPORT 18
1.2.2. PEPT1 GENE AND PROTEIN STRUCTURE 19
1.2.3. SUBSTRATES OF PEPT1 21
1.2.4. LOCALIZATION OF PEPT1 23
1.2.4.1. PEPT 1 EXPRESSION IN THE DIGESTIVE TRACT 23
1.2.4.2. EXPRESSION OF PEPT1 IN EXTRA-INTESTINAL TISSUE 24
1.2.5. REGULATION OF PEPT1 25
1.2.5.1. PEPT1 AND DIETARY FACTORS 25
1.2.5.2. HORMONAL CONTROL OF PEPT1 26
1.2.5.3. PEPT1 IN PATHOPHYSIOLOGICAL CONDITIONS 28
1.2.6. THE ROLE OF PEPT2 IN THE ORGANISM 28
1.3. PUTATIVE ROLE OF PEPT1 IN THE ORGANISM 29
1.4. AIM OF THE PROJECT 33
2. RESULTS 35
2.1. BASAL PHENOTYPIC CHARACTERIZATION OF PEPTF 1 MICE 35
2.1.1. BODY WEIGHT 35
2.1.2. HAPLOINSUFFICIENCY IN HETEROZYGOUS ANIMALS 36
2.1.3. CHARACTERIZATION OF TISSUE SAMPLES BY PROFILING TECHNIQUES 37
2.1.4. AMINO ACID TRANSPORT AND PEPTIDE TRANSPORTER EXPRESSION IN KIDNEY
TISSUE 40
2.1.5. BASAL METABOLIC PARAMETERS REVEAL ELEVATED GROSS ENERGY CONTENT
OF FECES IN MALE AND FEMALE PEPT1~ ~ ANIMALS 42
2.1.6. BASAL PLASMA GREHLIN, LEPTIN AND INSULIN LEVELS 43
HTTP://D-NB.INFO/1032616326
IMAGE 2
2.2. ANALYSIS OF SELECTED PARAMETERS IN BODY FLUIDS AND TISSUES IN BASAL
STATE 44
2.2.1. CLINICAL CHEMICAL PARAMETERS 44
2.2.2. BASAL AMINO ACID LEVELS IN PLASMA, URINE AND TISSUE IN MALE MICE
45
2.2.3. ENZYME AND UREA LEVELS IN LIVER 49
2.3. EFFECTS OF DIETARY PROTEIN INTAKE ON PHENOTYPIC CHANGES IN
PEPT1-DEFICIENT MICE 49
2.3.1. SUBTLE EFFECTS AFTER ADMINISTRATION OF A LOW-PROTEIN DIET 49
2.3.2. EFFECTS OF FEEDING A HIGH-PROTEIN DIET ON PEPTT 1 MICE 51
2.3.2.1. METABOLIC PARAMETERS 51
2.3.2.2. ENERGY CONTENT OF FECES IN PEPTT 1 MICE IS ELEVATED WHEN FED
A HP DIET 53
2.3.2.3. ENERGY ASSIMILATION DIFFERENCES 54
2.3.2.4. CLINICAL CHEMISTRY PLASMA AND URINE PARAMETERS IN ANIMALS FED A
HP DIET 54
2.3.2.5. HIGH-PROTEIN DIET ALTERS PLASMA AMINO ACID LEVELS 56
2.3.2.6. LIVER TISSUE AMINO ACID LEVELS AND ENZYME ACTIVITIES IN PEPTT 1
ANIMALS 60
2.3.2.7. DECREASED LEPTIN LEVELS IN PEPTT 1 ANIMALS 63
2.3.3. GENDER ASPECTS IN THE EFFECTS OF A HIGH-PROTEIN DIET 64
2.3.3.1. MODIFIED CLINICAL CHEMISTRY PLASMA PARAMETERS IN FEMALE PEPTT 1
MICE 65
2.3.3.2. ALTERATIONS IN PLASMA AND LIVER AMINO ACIDS OF FEMALE PEPTT 1
MICE ON A HIGH-PROTEIN DIET 65
2.3.3.3. ENZYME ACTIVITIES AND UREA CONCENTRATION IN LIVER TISSUE 68
2.3.4. EFFECTS OF A CARBOHYDRATE ADMINISTRATION 69
2.3.4.1. ADMINISTRATION OF A HIGH-PROTEIN DIET AND WATER ENRICHED WITH
GLUCOSE 69
2.3.4.2. BLOOD GLUCOSE AND INSULIN SECRETION AFTER AN ORAL GLUCOSE
TOLERANCE TEST 71
2.4. EFFECTS OF AN ACUTE PROTEIN ADMINISTRATION BY GAVAGE 72
2.4.1. CHANGES IN PLASMA AMINO ACIDS AFTER ADMINISTRATION OF A LOW DOSE
OF 15 N-LABELED PROTEIN 72
2.4.2. EFFECTS OF A HIGH INTRAGASTRIC PROTEIN LOAD ON PLASMA AND LIVER
AMINO ACID LEVELS IN PEPT1 +I+ AND PEPTT 1 ANIMALS 76
3. DISCUSSION 81
3.1. BASAL PHENOTYPIC CHARACTERIZATION 81
3.2. PEPT1 IN RESPONSE TO DIFFERENT PROTEIN CONTENTS 85
3.3. CONCLUSION 93
IMAGE 3
4. MATERIALS AND METHODS 97
4.1. CHEMICALS AND CONSUMABLES 97
4.2. LABORATORY ANIMALS 97
4.2.1. MOUSE HUSBANDRY 97
/
4.2.2. GENOTYPING 97
4.2.3. DATA SAMPLING FOR BODY WEIGHT 98
4.2.4. BOMB CALORIMETRY OF FECES SAMPLES 98
4.3. DIETARY INTERVENTION STUDIES 98
4.3.1. DIETARY INTERVENTION STUDY WITH LOW, MEDIUM AND HIGH PROTEIN
CONTENT FOR 5 DAYS IN MALE MICE 98
4.3.2. DIETARY INTERVENTION STUDY IN FEMALE MICE FOR 5 DAYS 99
4.3.3. DIETARY INTERVENTION STUDY FOR 18 DAYS 99
4.3.4. 24-HOUR DIETARY INTERVENTION STUDY 99
4.4. HIGH PROTEIN LOAD AND ADMINISTRATION OF LOW 15N-LABELED PROTEIN
LOAD BY GASTRIC GAVAGE 99
4.5. ANALYSIS OF SELECTED METABOLITES IN BODY FLUIDS AND TISSUE 100
4.5.1. CLINICAL CHEMICAL PARAMETERS IN PLASMA 100
4.5.2. AMINO ACID ANALYSIS IN PLASMA, URINE AND TISSUE 100
4.5.3. URINE PARAMETERS 100
4.5.4. DETERMINATION OF HORMONE LEVELS IN PLASMA 100
4.5.5. MEASUREMENT OF ENZYME ACTIVITES IN LIVER AND KIDNEY TISSUE 101
4.5.6. ORAL GLUCOSE TOLERANCE TEST (OGTT) AND INSULIN MEASUREMENT 101
4.6. CHARACTERIZATION OF TISSUE SAMPLES BY PROFILING TECHNIQUES 101
4.6.1. PROTEOME ANALYSIS OF INTESTINAL TISSUE BY 2D-PAGE AND
MALDI-TOF-MS 101
4.6.2. TRANSCRIPTOME ANALYSIS OF MUCOSAL SAMPLES BY CDNA MICROARRAYS 102
4.6.3. GENE EXPRESSION ANALYSIS BY QPCR 103
4.7. WESTERN BLOT ANALYSIS AND UPTAKE STUDIES IN BBMV AND EVERTED GUT
SACS 103
4.7.1. INTESTINAL BRUSH BORDER MEMBRANE VESICLES (BBMV) 103
4.7.2. PURITY OF BRUSH BORDER MEMBRANE VESICLES 103
4.7.3. PREPARATION OF BBMV FROM KIDNEY TISSUE AND UPTAKE STUDIES 104
4.7.4. WESTERN BLOT ANALYSIS 104
4.7.5. UPTAKE STUDIES BY THE EVERTED GUT SAC TECHNIQUE 104
IMAGE 4
4.8. TISSUE PREPARATION AND IMMMUNOFLUORESCENCE 105
4.9. DETECTION OF L)-GALACTOSIDASE ACTIVITY VIA X-GAL STAINING 105
4.10. STATISTICAL ANALYSIS 105
APPENDIX 107
REFERENCES 111
ERKLARUNG 120
CURRICULUM VITAE 122
DANKSAGUNG 125
|
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| spelling | Näßl, Anna-Maria Verfasser aut Amino acid absorption and homeostasis in mice lacking the intestinal peptide transporter PEPT1 Anna-Maria Näßl 2012 125 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. Sprache München, Techn. Univ., Diss., 2012 Peptidtransporter (DE-588)4759852-9 gnd rswk-swf Darm (DE-588)4011061-8 gnd rswk-swf Aminosäuren (DE-588)4142205-3 gnd rswk-swf Proteinstoffwechsel (DE-588)4014135-4 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Darm (DE-588)4011061-8 s Peptidtransporter (DE-588)4759852-9 s Aminosäuren (DE-588)4142205-3 s Proteinstoffwechsel (DE-588)4014135-4 s b DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:91-diss-20120508-1081579-1-0 http://mediatum.ub.tum.de/node?id=1081579 Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20120508-1081579-1-0 Resolving-System DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025089116&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Näßl, Anna-Maria Amino acid absorption and homeostasis in mice lacking the intestinal peptide transporter PEPT1 Peptidtransporter (DE-588)4759852-9 gnd Darm (DE-588)4011061-8 gnd Aminosäuren (DE-588)4142205-3 gnd Proteinstoffwechsel (DE-588)4014135-4 gnd |
| subject_GND | (DE-588)4759852-9 (DE-588)4011061-8 (DE-588)4142205-3 (DE-588)4014135-4 (DE-588)4113937-9 |
| title | Amino acid absorption and homeostasis in mice lacking the intestinal peptide transporter PEPT1 |
| title_auth | Amino acid absorption and homeostasis in mice lacking the intestinal peptide transporter PEPT1 |
| title_exact_search | Amino acid absorption and homeostasis in mice lacking the intestinal peptide transporter PEPT1 |
| title_full | Amino acid absorption and homeostasis in mice lacking the intestinal peptide transporter PEPT1 Anna-Maria Näßl |
| title_fullStr | Amino acid absorption and homeostasis in mice lacking the intestinal peptide transporter PEPT1 Anna-Maria Näßl |
| title_full_unstemmed | Amino acid absorption and homeostasis in mice lacking the intestinal peptide transporter PEPT1 Anna-Maria Näßl |
| title_short | Amino acid absorption and homeostasis in mice lacking the intestinal peptide transporter PEPT1 |
| title_sort | amino acid absorption and homeostasis in mice lacking the intestinal peptide transporter pept1 |
| topic | Peptidtransporter (DE-588)4759852-9 gnd Darm (DE-588)4011061-8 gnd Aminosäuren (DE-588)4142205-3 gnd Proteinstoffwechsel (DE-588)4014135-4 gnd |
| topic_facet | Peptidtransporter Darm Aminosäuren Proteinstoffwechsel Hochschulschrift |
| url | http://mediatum.ub.tum.de/node?id=1081579 https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20120508-1081579-1-0 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025089116&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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