The role of CCL17 in murine experimental colitis:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online-Zugang: | Volltext https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20120229-1096018-1-1 Inhaltsverzeichnis |
| Beschreibung: | XI, 90 Bl. graph. Darst. |
Internformat
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Datensatz im Suchindex
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|---|---|
| adam_text | IMAGE 1
TABLE OF CONTENTS
TABLE OF CONTENTS
TABLE OF CONTENTS I
INDEX OF FIGURES IV
INDEX OF TABLES VI
ABBREVIATIONS VII
1 INTRODUCTION 1
1.1 THE IMMUNE SYSTEM 1
1.1.1 THE INNATE IMMUNE SYSTEM 1
1.1.2 THE ADAPTIVE IMMUNE SYSTEM 2
1.2 GUT-ASSOCIATED LYMPHOID TISSUE (GALT) 6
1.3 CHRONIC INTESTINAL INFLAMMATION / INFLAMMATORY BOWEL DISEASES (IBD)
10
1.3.1.1 GENETIC FACTORS DRIVING IBD 10
1.3.1.2 ENVIRONMENTAL FACTORS DRIVING IBD 11
1.3.1.3 DYSREGULATION OF THE INTESTINAL IMMUNE RESPONSE IN IBD 12
1.3.1.4 CHEMOKINES IN IBD 16
1.4 CHEMOKINE (C-C MOTIF) LIGAND 17 (CCL17) 17
2 AIMS O F THE STUDY 20
3 MATERIAL AND METHODS 21
3.1 MATERIAL 21
3.1.1 EQUIPMENT 21
3.1.2 REAGENTS 23
3.1.3 KITS AND ENZYMES 25
3.1.4 ANTIBODIES FOR FLUORESCENT ACTIVATED CELL SORTING (FACS) AND
ENZYME LINKED
IMMUNOSORBENT ASSAY (ELISA) 25
3.1.5 MEDIA AND BUFFERS 27
3.1.5.1 MEDIA FOR CELL CULTURE 27
3.1.5.2 BUFFERS FOR ENZYME LINKED IMMUNOSORBENT ASSAY (ELISA) 27
I
HTTP://D-NB.INFO/1032532734
IMAGE 2
TABLE OF CONTENTS
3.1.5.3 BUFFER FOR MAGNETIC ACTIVATED CELL SORTING (MACS) 28
3.1.5.4 BUFFER FOR FLUORESCENT ACTIVATED CELL SORTING (FACS) 28
3.2 METHODS 29
3.2.1 MICE 29
3.2.2 COLITIS INDUCTION 30
3.2.3 ISOLATION O F COLON LAMINA PROPIA LEUKOCYTES (LPL) AND
INTRAEPITHELIAL
LEUKOCYTES (IEL) 30
3.2.4 GENERATION OF BONE MARROW DERIVED RECOMBINANT MOUSE (RM)GMCSF
CULTURED DENDRITIC CELLS (BMDCS) 30
3.2.5 ENZYME LINKED IMMUNOSORBENT ASSAY (EL1SA) 31
3.2.6 CELL ISOLATION AND MAGNETIC ACTIVATED CELL SORTING (MACS) 32
3.2.7 FLUORESCENCE ACTIVATED CELL SORTING (FACS) 33
3.2.8 IN VITRO STIMULATION AND COCULTURE 33
3.2.9 RNA ISOLATION AND REAL-TIME QUANTITATIVE PCR (QPCR) 34.
3.2.10 HISTOLOGY 34
3.2.11 PERITONEAL LAVAGE 35
3.2.12 STATISTICAL ANALYSIS 35
4 RESULTS 36
4.1 INDUCTION OF ACUTE AND CHRONIC INTESTINAL INFLAMMATION IN MICE 36
4.2 INDUCTION OF CCL17 DURING ACUTE AND CHRONIC INTESTINAL INFLAMMATION
37
4.3 REDUCED COLITIS INDUCTION IN MICE LACKING CELL 7 39
4.4 PROTECTION FROM SEVERE COLITIS IN LYMPHOPENIC MICE AFTER TRANSFER OF
CCR4~ ~ T
CELLS 45
4.5 ENHANCEMENT O F DC-CYTOKINE PRODUCTION BY CCL17 49
4.6 T CELL ACTIVATION BY WT AND CELL 7 ^ DCS 52
4.7 FOXP3-DEPENDENT LONGTERM PROTECTION OF RAGL 1 CELL 7 E/E MICE 56
5 DISCUSSION 64
5.1 COLITIS INDUCTION METHOD 65
II
IMAGE 3
TABLE OF CONTENTS
5.1.1 T CELL TRANSFER COLITIS 65
5.1.2 DEXTRAN SULFATE SODIUM (DSS) COLITIS 66
5.2 CCL17-DEPENDENT COLITIS ACTIVITY 67
5.2.1 CLINICAL AND HISTOLOGICAL ASSESSMENT OF COLITIS ACTIVITY 67
5.2.2 ROLE OF CYTOKINES AND T CELL DIFFERENTIATION 68
5.2.3 RECRUITMENT OF INFLAMMATORY CELLS 71
5.3 LONGTERM REGULATION OF INFLAMMATION IN THE ABSENCE OF CCL17 72
5.4 PROPOSED MODEL FOR THE ROLE OF CCL17 IN INTESTINAL INFLAMMATION 74
6 SUMMARY 76
7 REFERENCES 78
8 ACKNOWLEDGEMENTS 88
9 CURRICULUM VITAE 89
III
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| discipline | Biologie Medizin |
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| spelling | Heiseke, Alexander Frank Verfasser aut The role of CCL17 in murine experimental colitis Alexander Frank Heiseke 2012 XI, 90 Bl. graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Techn. Univ., Diss., 2012 Entzündung (DE-588)4014975-4 gnd rswk-swf Chemokine (DE-588)4429989-8 gnd rswk-swf Dickdarmentzündung (DE-588)4149633-4 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Dickdarmentzündung (DE-588)4149633-4 s Chemokine (DE-588)4429989-8 s Entzündung (DE-588)4014975-4 s b DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:91-diss-20120229-1096018-1-1 http://mediatum.ub.tum.de/node?id=1096018 Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20120229-1096018-1-1 Resolving-System DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025087617&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Heiseke, Alexander Frank The role of CCL17 in murine experimental colitis Entzündung (DE-588)4014975-4 gnd Chemokine (DE-588)4429989-8 gnd Dickdarmentzündung (DE-588)4149633-4 gnd |
| subject_GND | (DE-588)4014975-4 (DE-588)4429989-8 (DE-588)4149633-4 (DE-588)4113937-9 |
| title | The role of CCL17 in murine experimental colitis |
| title_auth | The role of CCL17 in murine experimental colitis |
| title_exact_search | The role of CCL17 in murine experimental colitis |
| title_full | The role of CCL17 in murine experimental colitis Alexander Frank Heiseke |
| title_fullStr | The role of CCL17 in murine experimental colitis Alexander Frank Heiseke |
| title_full_unstemmed | The role of CCL17 in murine experimental colitis Alexander Frank Heiseke |
| title_short | The role of CCL17 in murine experimental colitis |
| title_sort | the role of ccl17 in murine experimental colitis |
| topic | Entzündung (DE-588)4014975-4 gnd Chemokine (DE-588)4429989-8 gnd Dickdarmentzündung (DE-588)4149633-4 gnd |
| topic_facet | Entzündung Chemokine Dickdarmentzündung Hochschulschrift |
| url | http://mediatum.ub.tum.de/node?id=1096018 https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20120229-1096018-1-1 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025087617&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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