Odontogenesis: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Humana Press, Springer
2012
|
| Schriftenreihe: | Methods in molecular biology
887 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Klappentext |
| Beschreibung: | X, 194 S. Ill., graph. Darst. |
| ISBN: | 9781617798597 |
Internformat
MARC
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| 245 | 1 | 0 | |a Odontogenesis |b methods and protocols |c ed. by Chrissa Kioussi |
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Datensatz im Suchindex
| _version_ | 1804149164956712960 |
|---|---|
| adam_text | Contents
Preface
..................................................................
χ>
Contributors
..............................................................
¿χ
1
Histological Analysis of the Embryonic and Adult Tooth
.................... 1
Atsushi Ohazama
2
Determination of Gene Expression Patterns by Whole-Mount
In Situ Hybridization
............................................... 15
Sergiy KyryachenkOy KaUryna Kyrylkova, Mark
Leid,
and Chrissa Kiomsi
3
Determination of Gene Expression Patterns by In Situ
Hybridization in Sections
............................................ 23
Kateryna Kyrylkova, Sergiy
Kyryachenko,
Chrissa Kioussi, and Mark
Leid
4
Immunohistochemistry and Detection of Proliferating Cells by BrdU
........... 33
Sergiy Kyryachenko, Kateryna Kyrylkova, Mark
Leid,
and Chrissa Kioussi
5
Detection ofApoptosis by
TUNEL
Assay
................................ 41
Kateryna Kyrylkova, Sergiy Kyryachenko, Mark
Leid,
and Chrissa Kioussi
6
Use of si
RNA
in Dental Tissue-Derived Cell Cultures:
Integrin
Knockdown in
Fibroblasts
..................................... 49
Małgorzata
M.
Barczyk,
Donald
Gullberg, and
Anne Isine
Bolstad
7
Organ Cultures and Kidney-Capsule Grafting of Tooth Germs
................ 59
Keishi Otsu, Naoki
Fujiwar
a, and Hidemitsu Harada
8
Evaluation of Skull and Tooth Morphology and Mineralization
Using High-Resolution
Х
-Ray Tomography
.............................. 69
Brian K. Bay
9
Electron Microscopy
................................................ 81
Hans U.
Luder
and Margrit Amstad-Jossi
10
Deoxyoligonucleotide Microarrays for Gene Expression Profiling
in
Murine
Tooth Germs
............................................. 95
Harald Osmundsen,
Anne-Martbe Jevnaker, and Maria A.
Landin
1
1
Lineage Differentiation of Mesenchymal Stem Cells from Dental Pulp,
Apical Papilla, and Periodontal ligament
................................
Ill
Kentoro Akiyama, Cinder Chen, Stan Grontbos, and Songtao Shi
12
In Vivo Transplantation and Tooth Repair
............................... 123
Sbuhei Tsucbiya and MasakiJ. Honda
13
Methods to Validate Tooth-Supporting Regenerative Therapies
............... 135
Miguel Padial-Molina, Julie T. Marcbesan, Andrei D. Taut,
Qimingjin, William V. Giannobile, and Hector F.
Rios
14
Generation of a Bioengineered Tooth by Using a Three-Dimensional
Cell Manipulation Method (Organ Germ Method)
......................... 149
Masamitsu Oshima, Mibo Ogawa, Masato Tasukawa, and Takasbi
Tsuß
VU
viii Contents
15 In Vitro
Studies on
Odontogenic Tumors................................ 167
Javier
Catón,
ThimiosA. Mitsiadis, and Peter R. Morgan
16
Whole Mount Immunohistochemistry and In Situ Hybridization
of Larval and Adult Zebrafish Dental Tissues
............................. 179
Barbara Verstraeten, Ellen Sanders, and Ann Huysseune
Index
................................................................... 193
Methods in Molecular Biology
887
John M. Walker, Series Editor
Chrissa Kioussi Editor
Odontogenesis
Methods and Protocols
Only in recent times has the possibility of growing and implanting replacement teeth, made from
one s own cells, moved into the realm ot realistic possibilities; however, the molecular and cellular
mechanisms ot tooth development must be studied in a range of vertebrates, from zebrafish to
mice, so that evolutionarily conserved network kernels, which will define the cellular states ot ge¬
neric vertebrate tooth development, can be recogni/ed. In Odontogenesis: Methods and Protocols,
experts in the field examine techniques to approach this burgeoning field. This detailed volume
includes chapters on the detection of tooth development gene expression, both at the RXA and
protein level, current approaches to the manipulation of gene expression levels and subsequent
analysis ot tooth phenotypes, as well as chapters concerning current etforts to get living tooth
implants working without waiting tor a full understanding of the developmental pathways at the
molecular level. Written in the highly successful Methods in
Molecular
Biology series format,
chapters include introductions to their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols, and tips for trouble-shooting
and avoiding known pitfalls.
Practical and easy to use, Odontogenesis: Methods and Protocols aims to help researchers move
forward toward the ultimate goal of getting a bioengineered tooth into the patients mouth.
|
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| indexdate | 2024-07-10T00:18:41Z |
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| isbn | 9781617798597 |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Odontogenesis methods and protocols ed. by Chrissa Kioussi New York [u.a.] Humana Press, Springer 2012 X, 194 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 887 Analyse (DE-588)4122795-5 gnd rswk-swf Zahnentwicklung (DE-588)4079450-7 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Zahnentwicklung (DE-588)4079450-7 s Analyse (DE-588)4122795-5 s DE-604 Kioussi, Chrissa Sonstige oth Erscheint auch als Online-Ausgabe 978-1-61779-860-3 Methods in molecular biology 887 (DE-604)BV035362695 887 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025001597&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025001597&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Klappentext |
| spellingShingle | Odontogenesis methods and protocols Methods in molecular biology Analyse (DE-588)4122795-5 gnd Zahnentwicklung (DE-588)4079450-7 gnd |
| subject_GND | (DE-588)4122795-5 (DE-588)4079450-7 (DE-588)4143413-4 |
| title | Odontogenesis methods and protocols |
| title_auth | Odontogenesis methods and protocols |
| title_exact_search | Odontogenesis methods and protocols |
| title_full | Odontogenesis methods and protocols ed. by Chrissa Kioussi |
| title_fullStr | Odontogenesis methods and protocols ed. by Chrissa Kioussi |
| title_full_unstemmed | Odontogenesis methods and protocols ed. by Chrissa Kioussi |
| title_short | Odontogenesis |
| title_sort | odontogenesis methods and protocols |
| title_sub | methods and protocols |
| topic | Analyse (DE-588)4122795-5 gnd Zahnentwicklung (DE-588)4079450-7 gnd |
| topic_facet | Analyse Zahnentwicklung Aufsatzsammlung |
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