Human embryonic stem cells handbook:
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Humana Press, Springer
2012
|
| Schriftenreihe: | Methods in molecular biology
873 |
| Schlagworte: | |
| Online-Zugang: | Klappentext Inhaltsverzeichnis |
| Beschreibung: | XI, 313 S. Ill., graph. Darst. 24 cm |
| ISBN: | 1617797936 9781617797934 |
Internformat
MARC
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| 245 | 1 | 0 | |a Human embryonic stem cells handbook |c ed. by Kursad Turksen |
| 264 | 1 | |a New York [u.a.] |b Humana Press, Springer |c 2012 | |
| 300 | |a XI, 313 S. |b Ill., graph. Darst. |c 24 cm | ||
| 336 | |b txt |2 rdacontent | ||
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Datensatz im Suchindex
| _version_ | 1823056931260989440 |
|---|---|
| adam_text |
Contents
Preface
.
v
Contributors.
. ix
1
Establishment of New Lines of Human Embryonic Stem Cells:
Evolution of the Methodology
. 1
Ana Maria
Vraga,
Èrica
Sara
Souza de Araújo,
Raquel
Stabellini, Naja
Vergani,
and Lygia V.
Pereira
2
Human Embryonic Stem Cells Derived in Xeno-Free Conditions
. 13
Amparo
Galán
and Carlos
Simón
3
Procedures for Derivation and Characterisation of Human
Embryonic Stem Cells from
Odense,
Denmark
. 33
Linda Harkness and Moustapha Kassem
4
Principles for Derivation of Human Embryonic Stem Cells
. 53
Mikael
CO.
Englund, Catharina Ellerström,
Katarina Andersson, Karin Noaksson, and Johan Hyllner
5
Derivation and Maintenance of
Undifferentiated Human
Embryonic Stem
Cells
. 69
Guoliang
Meng
and Derrick
E.
Rancourt
6 Establishment
of hESC Lines
from the
Inner
Cell Mass
of Blastocyst-Stage Embryos
and Single Blastomeres
of 4-Cell Stage
Embryos
. 81
Ileana
Mateizel, Mieke
Geens, Hilde
Van de Veide,
and Karen
Sermon
7
Analysis
of LINE-
1 Expression
in Human Pluripotent Cells
. 113
Martin
Muñoz-Lopez,
Marta
Garcia-Cañadas,
Angela
Macia,
Santiago
Moreli,
and
Jose
L· Garcia-Perez
8
Characterization and Gene Expression Profiling of Five Human
Embryonic Stem Cell Lines Derived in Taiwan
. 127
Steven Shoei-Lung Li
9
Derivation of Human Embryonic Stem Cell Lines
from Poor Quality Embryos
. 151
Ronak Shetty and Maneesha S. Inamdar
10
Derivation, Expansion, and Characterization of Human Embryonic
Stem Cell Lines from Aneuploid Embryos
. 163
Juan-Carlos
Bianconi
and
Neta Lavon
11
Mutated Human Embryonic Stem Cells for the Study
of Human Genetic Disorders
. 179
Ahmt Ben-Tehudah, Mira Malcov,
Tsvia Frumkin,
and Dalit Ben-Tosef
VII
viii Contents
12
Single-Cell Enzymatic Dissociation of hESC Lines OXF1-OXF4
and Culture in Feeder-Free Conditions
. 209
Frances A. Brook
13
Protocol for Expansion of Undifferentiated Human Embryonic
and Pluripotent Stem Cells in Suspension
. 217
Hossein Baharvand,
Mebran Rezaei Larijani,
and Maryam Yousefi
14
Suspension Bioreactor Expansion of Undifferentiated Human
Embryonic Stem Cells
. 227
Roman Krawetz and Derrick E. Rancourt
15
Derivation, Propagation, and Characterization of Neuroprogenitors
from Pluripotent Stem Cells (hESCs and hiPSCs)
. 237
Khun-Hong Lie, Henry C.Y. Chung, andKuldip S. Sidhu
16
Comparison of Neural Differentiation Potential of Human
Pluripotent Stem Cell Lines Using a Quantitative Neural
Differentiation Protocol
. 247
Oezhong Tin, Tahereh Tavakoli, Wei-Qiang
Gao,
and Wu Ma
17
Array-Comparative Genomic Hybridization Characterization
of Human Pluripotent Stem Cells
. 261
Aaron M. Elliott,
Kristi
A. Hohenstein Elliott,
and
Anja Kammesheidt
18
Chromatin Immunoprecipitation-Based Analysis of Gene Regulatory
Networks Operative in Human Embryonic Stem Cells
. 269
Marc Jung and James Adjaye
19
Analysis of the Methylome of Human Embryonic Stem Cells
Employing Methylated
DNA Immunoprecipitation
Coupled
to Next-Generation Sequencing
. 281
Christina Grimm and James Adjaye
20
Stable Isotope Labelling with
Amino
Acids in Cell Culture
for Human Embryonic Stem Cell Proteomic Analysis
. 297
Linda Harkness, Tatyana A. Prokhorova, Moustapha Kassem,
and Blagoy Blagoev
Index
. 307
^Considerable advances have taken place since the initial isolation and characterization of human
ş
I^mbryonic stem
(HES)
cells; however, significant challenges remain before their potential
foii
I restoration and
ŕégenératipn
processes in patients can be realized/Understanding the diversity!
i! 'amongst
HES
cell
lineš
and realizing
thé
ability to isolate lines with robust differentiation potent
Ştiai
remain difficult.in the Human
EmbryoţiicStem
Се
/is
Handboofc, experts in the field provide
Ї
Sán
assortment of protocols that have been used by various laboratories around the world so as to
?
ţallow
both novices and experienced investigators to compare and contrast different approaches .|
po HES
cell isolation and characterization with the hope that, from these
prótocQkřresearcheretj
í;might
standardize approaches for
HES
cell biology. Written in the
ЈИе/иоЉ
»и
Afb/ecu/ar
βίο-;·;
pìogyaséries
format^ chapters include introductions to their
respective
topics
listó oü
¿sary materials and reagents, step-t
iv
troubleshooting and avoiding known pitfalls;
:||
eMthoritativeäridäccéssiblei1/'
'' '
^
--»—;-;;»;·
luable
ice for scientists pursuing this vital field and its enormous
potenţialii |
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| id | DE-604.BV040144712 |
| illustrated | Illustrated |
| indexdate | 2025-02-03T17:09:32Z |
| institution | BVB |
| isbn | 1617797936 9781617797934 |
| language | English |
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| physical | XI, 313 S. Ill., graph. Darst. 24 cm |
| publishDate | 2012 |
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| publisher | Humana Press, Springer |
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| series | Methods in molecular biology |
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| spelling | Human embryonic stem cells handbook ed. by Kursad Turksen New York [u.a.] Humana Press, Springer 2012 XI, 313 S. Ill., graph. Darst. 24 cm txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 873 Springer protocols Embryonale Stammzelle (DE-588)4699648-5 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf Tissue Engineering (DE-588)4646061-5 gnd rswk-swf Embryonic stem cells. Human embryo. (DE-588)4143413-4 Aufsatzsammlung gnd-content Embryonale Stammzelle (DE-588)4699648-5 s Labortechnik (DE-588)4123602-6 s DE-604 Tissue Engineering (DE-588)4646061-5 s b DE-604 Turksen, Kursad edt Erscheint auch als Online-Ausgabe 978-1-61779-794-1 Methods in molecular biology 873 (DE-604)BV035362695 873 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025001557&sequence=000005&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Klappentext Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025001557&sequence=000006&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Human embryonic stem cells handbook Methods in molecular biology Embryonale Stammzelle (DE-588)4699648-5 gnd Labortechnik (DE-588)4123602-6 gnd Tissue Engineering (DE-588)4646061-5 gnd |
| subject_GND | (DE-588)4699648-5 (DE-588)4123602-6 (DE-588)4646061-5 (DE-588)4143413-4 |
| title | Human embryonic stem cells handbook |
| title_auth | Human embryonic stem cells handbook |
| title_exact_search | Human embryonic stem cells handbook |
| title_full | Human embryonic stem cells handbook ed. by Kursad Turksen |
| title_fullStr | Human embryonic stem cells handbook ed. by Kursad Turksen |
| title_full_unstemmed | Human embryonic stem cells handbook ed. by Kursad Turksen |
| title_short | Human embryonic stem cells handbook |
| title_sort | human embryonic stem cells handbook |
| topic | Embryonale Stammzelle (DE-588)4699648-5 gnd Labortechnik (DE-588)4123602-6 gnd Tissue Engineering (DE-588)4646061-5 gnd |
| topic_facet | Embryonale Stammzelle Labortechnik Tissue Engineering Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025001557&sequence=000005&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025001557&sequence=000006&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
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