Molecular fluorescence: principles and applications
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Weinheim
Wiley-VCH
2012
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| Ausgabe: | 2. ed. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XXI, 569 S. Ill., graph. Darst. |
| ISBN: | 9783527328376 9783527328468 9783527650033 |
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| 100 | 1 | |a Valeur, Bernard |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Molecular fluorescence |b principles and applications |c Bernard Valeur and Mário Nuno Berberan-Santos |
| 250 | |a 2. ed. | ||
| 264 | 1 | |a Weinheim |b Wiley-VCH |c 2012 | |
| 300 | |a XXI, 569 S. |b Ill., graph. Darst. | ||
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| 700 | 1 | |a Berberan-Santos, Mário Nuno |e Verfasser |4 aut | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024997404&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
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| adam_text | IMAGE 1
CONTENTS
PREFACE TO THE FIRST EDITION XV PREFACE TO THE SECOND EDITION X V I I
ACKNOWLEDGMENTS X I X
PROLOGUE X X I
1 INTRODUCTION 1
1.1 WHAT IS LUMINESCENCE? 1 1.2 A BRIEF HISTORY O F FLUORESCENCE AND
PHOSPHORESCENCE 2 1.2.1 EARLY OBSERVATIONS 3 1.2.2 ON THE DISTINCTION
BETWEEN FLUORESCENCE AND
PHOSPHORESCENCE: DECAY TIME MEASUREMENTS 10 1.2.3 THE PERRIN-JABLONSKI
DIAGRAM 12 1.2.4 FLUORESCENCE POLARIZATION 14 1.2.5 RESONANCE ENERGY
TRANSFER 16 1.2.6 EARLY APPLICATIONS O F FLUORESCENCE 17 1.3
PHOTOLUMINESCENCE OF ORGANIC AND INORGANIC SPECIES:
FLUORESCENCE OR PHOSPHORESCENCE? 19 1.4 VARIOUS DE-EXCITATION PROCESSES
O F EXCITED MOLECULES 20 1.5 FLUORESCENT PROBES, INDICATORS, LABELS, AND
TRACERS 21 1.6 ULTIMATE TEMPORAL AND SPATIAL RESOLUTION: FEMTOSECONDS,
FEMTOLITERS, FEMTOMOLES, AND SINGLE-MOLECULE DETECTION 23 GENERAL
BIBLIOGRAPHY: MONOGRAPHS AND BOOKS 25
PART I PRINCIPLES 31
2 ABSORPTION O F ULTRAVIOLET, VISIBLE, AND NEAR-INFRARED RADIATION 33
2.1 ELECTRONIC TRANSITIONS 33 2.2 TRANSITION PROBABILITIES: THE
BEER-LAMBERT LAW, OSCILLATOR STRENGTH 39
2.3 SELECTION RULES 46 2.4 THE FRANCK-CONDON PRINCIPLE 47
HTTP://D-NB.INFO/1016558465
IMAGE 2
V I CONTENTS
2.5 MULTIPHOTON ABSORPTION AND HARMONIC GENERATION 49
BIBLIOGRAPHY 53
3 CHARACTERISTICS OF FLUORESCENCE EMISSION 53 3.1 RADIATIVE AND
NONRADIATIVE TRANSITIONS BETWEEN ELECTRONIC STATES 53 3.1.1 INTERNAL
CONVERSION 56 3.1.2 FLUORESCENCE 56
3.1.3 INTERSYSTEM CROSSING AND SUBSEQUENT PROCESSES 57 3.1.3.1
INTERSYSTEM CROSSING 58 3.1.3.2 PHOSPHORESCENCE VERSUS NONRADIATIVE
DE-EXCITATION 60 3.1.3.3 DELAYED FLUORESCENCE 60
3.1.3.4 TRIPLET-TRIPLET TRANSITIONS 63 3.2 LIFETIMES AND QUANTUM YIELDS
63 3.2.1 EXCITED-STATE LIFETIMES 63 3.2.2 QUANTUM YIELDS 64
3.2.3 EFFECT O F TEMPERATURE 66 3.3 EMISSION AND EXCITATION SPECTRA 67
3.3.1 STEADY-STATE FLUORESCENCE INTENSITY 67 3.3.2 EMISSION SPECTRA 68
3.3.3 EXCITATION SPECTRA 73 3.3.4 STOKES SHIFT 72 BIBLIOGRAPHY 74
4 STRUCTURAL EFFECTS ON FLUORESCENCE EMISSION 75 4.1 EFFECTS OF THE
MOLECULAR STRUCTURE O F ORGANIC MOLECULES ON THEIR FLUORESCENCE 75 4.1.1
EXTENT O F THE IT-ELECTRON SYSTEM: NATURE O F THE LOWEST-LYING
TRANSITION 75 4.1.2 SUBSTITUTED AROMATIC HYDROCARBONS 77 4.1.2.1
INTERNAL HEAVY ATOM EFFECT 77 4.1.2.2 ELECTRON-DONATING SUBSTITUENTS: -
O H , -OR, -NH 2 ,
-NHR, -NR 2 78 4.1.2.3 ELECTRON-WITHDRAWING SUBSTITUENTS: CARBONYL AND
NITRO COMPOUNDS 78 4.1.2.4 SULFONATES 79 4.1.3 HETEROCYCLIC COMPOUNDS 80
4.1.3.1 COMPOUNDS WITH HETERONITROGEN ATOMS 80 4.1.3.2 COUMARINS 81
4.1.3.3 XANTHENIC DYES 82 4.1.3.4 OXAZINES 84 4.1.3.5 CYANINES 85
4.1.3.6 BODIPY FLUOROPHORES 86 4.1.4 COMPOUNDS UNDERGOING PHOTOINDUCED
ICT AND
INTERNAL ROTATION 87
IMAGE 3
CONTENTS | V I I
4.2 FLUORESCENCE O F CONJUGATED POLYMERS (CPS) 92
4.3 LUMINESCENCE O F CARBON NANOSTRUCTURES: FULLERENES, NANOTUBES, AND
CARBON DOTS 93 4.4 LUMINESCENCE O F METAL COMPOUNDS, METAL COMPLEXES,
AND METAL CLUSTERS 96 4.5 LUMINESCENCE O F SEMICONDUCTOR NANOCRYSTALS
(QUANTUM DOTS AND
QUANTUM RODS) 103 BIBLIOGRAPHY 105
5 ENVIRONMENTAL EFFECTS ON FLUORESCENCE EMISSION 109 5.1 HOMOGENEOUS AND
INHOMOGENEOUS BAND BROADENING-RED-EDGE EFFECTS 109 5.2 GENERAL
CONSIDERATIONS ON SOLVENT EFFECTS 110 5.3 SOLVENT RELAXATION SUBSEQUENT
TO PHOTOINDUCED CHARGE
TRANSFER (PCT) 112 5.4 THEORY OF SOLVATOCHROMIC SHIFTS 117 5.5 EFFECTS O
F SPECIFIC INTERACTIONS 119 5.5.1 EFFECTS OF HYDROGEN BONDING ON
ABSORPTION AND FLUORESCENCE
SPECTRA 119
5.5.2 EXAMPLES O F EFFECTS O F SPECIFIC INTERACTIONS 120 5.5.3
POLARITY-INDUCED INVERSION O F N-7T* AND N-JT* STATES 123 5.6 EMPIRICAL
SCALES O F SOLVENT POLARITY 124 5.6.1 SCALES BASED ON SOLVATOCHROMIC
SHIFTS 124
5.6.1.1 SINGLE-PARAMETER APPROACH 124 5.6.1.2 MULTIPARAMETER APPROACH
126 5.6.2 SCALE BASED ON POLARITY-INDUCED CHANGES IN VIBRONIC BANDS (PY
SCALE) 129 5.7 VISCOSITY EFFECTS 129
5.7.1 WHAT IS VISCOSITY? SIGNIFICANCE AT A MICROSCOPIC LEVEL 129 5.7.2
VISCOSITY EFFECT ON THE FLUORESCENCE O F MOLECULES UNDERGOING INTERNAL
ROTATIONS 132 5.8 FLUORESCENCE IN SOLID MATRICES AT LOW TEMPERATURE 135
5.8.1 SHPOL SKII SPECTROSCOPY 136
5.8.2 MATRIX ISOLATION SPECTROSCOPY 137 5.8.3 SITE-SELECTION
SPECTROSCOPY 137 5.9 FLUORESCENCE IN GAS PHASE: SUPERSONIC JETS 137
BIBLIOGRAPHY 138
6 EFFECTS O F INTERMOLECULAR PHOTOPHYSICAL PROCESSES ON FLUORESCENCE
EMISSION 141 6.1 INTRODUCTION 141 6.2 OVERVIEW O F THE INTERMOLECULAR
DE-EXCITATION PROCESSES O F EXCITED
MOLECULES LEADING TO FLUORESCENCE QUENCHING 143 6.2.1 PHENOMENOLOGICAL
APPROACH 143
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V I I I CONTENTS
6.2.2 DYNAMIC QUENCHING 146
6.2.2.1 STERN-VOLMER KINETICS 146 6.2.2.2 TRANSIENT EFFECTS 148 6.2.3
STATIC QUENCHING 152 6.2.3.1 SPHERE O F EFFECTIVE QUENCHING 152
6.2.3.2 FORMATION OF A GROUND-STATE NONFLUORESCENT COMPLEX 153 6.2.4
SIMULTANEOUS DYNAMIC AND STATIC QUENCHING 154 6.2.5 QUENCHING O F
HETEROGENEOUSLY EMITTING SYSTEMS 158 6.3 PHOTOINDUCED ELECTRON TRANSFER
159 6.4 FORMATION OF EXCIMERS AND EXCIPLEXES 162
6.4.1 EXCIMERS 163 6.4.2 EXCIPLEXES 167 6.5 PHOTOINDUCED PROTON TRANSFER
168 6.5.1 GENERAL EQUATIONS FOR DEPROTONATION IN THE EXCITED STATE 170
6.5.2 DETERMINATION O F THE EXCITED-STATE PK* 172 6.5.2.1 PREDICTION BY
MEANS O F THE FORSTER CYCLE 172 6.5.2.2 STEADY-STATE MEASUREMENTS 173
6.5.2.3 TIME-RESOLVED EXPERIMENTS 174
6.5.3 PH DEPENDENCE O F ABSORPTION AND EMISSION SPECTRA 174 6.5.4
EQUATIONS FOR BASES UNDERGOING PROTONATION IN THE EXCITED STATE 178
BIBLIOGRAPHY 179
7 FLUORESCENCE POLARIZATION: EMISSION ANISOTROPY 181 7.1 POLARIZED LIGHT
AND PHOTOSELECTION O F ABSORBING MOLECULES 181 7.2 CHARACTERIZATION OF
THE POLARIZATION STATE OF FLUORESCENCE (POLARIZATION RATIO AND EMISSION
ANISOTROPY) 184
7.2.1 EXCITATION BY POLARIZED LIGHT 184 7.2.1.1 VERTICALLY POLARIZED
EXCITATION 184 7.2.1.2 HORIZONTALLY POLARIZED EXCITATION 186
7.2.2 EXCITATION BY NATURAL LIGHT 187 7.3 INSTANTANEOUS AND STEADY-STATE
ANISOTROPY 187 7.3.1 INSTANTANEOUS ANISOTROPY 187
7.3.2 STEADY-STATE ANISOTROPY 188 7.4 ADDITIVITY LAW OF ANISOTROPY 188
7.5 RELATION BETWEEN EMISSION ANISOTROPY AND ANGULAR DISTRIBUTION OF THE
EMISSION TRANSITION MOMENTS 190 7.6 CASE O F MOTIONLESS MOLECULES WITH
RANDOM ORIENTATION 191
7.6.1 PARALLEL ABSORPTION AND EMISSION TRANSITION MOMENTS 191 7.6.2
NONPARALLEL ABSORPTION AND EMISSION TRANSITION MOMENTS 192 7.6.3
MULTIPHOTON EXCITATION 196 7.7 EFFECT OF ROTATIONAL MOTION 199
7.7.1 FREE ROTATIONS 200 7.7.1.1 GENERAL EQUATIONS 200
IMAGE 5
CONTENTS | I X
7.7.1.2 ISOTROPIC ROTATIONS 201
7.7.1.3 ANISOTROPIC ROTATIONS 203 7.7.2 HINDERED ROTATIONS 206 7.8
APPLICATIONS 207 BIBLIOGRAPHY 210
8 EXCITATION ENERGY TRANSFER 213 8.1 INTRODUCTION 213 8.2 DISTINCTION
BETWEEN RADIATIVE AND NONRADIATIVE TRANSFER 218 8.3 RADIATIVE ENERGY
TRANSFER 219 8.4 NONRADIATIVE ENERGY TRANSFER 221
8.4.1 INTERACTIONS INVOLVED IN NONRADIATIVE ENERGY TRANSFER 221 8.4.2
THE THREE MAIN CLASSES OF COUPLING 224 8.4.3 FORSTER S FORMULATION O F
LONG-RANGE DIPOLE-DIPOLE TRANSFER (VERY WEAK COUPLING) 226
8.4.4 DEXTER S FORMULATION OF EXCHANGE ENERGY TRANSFER (VERY WEAK
COUPLING) 233 8.4.5 SELECTION RULES 233 8.5 DETERMINATION OF DISTANCES
AT A SUPRAMOLECULAR LEVEL USING
FRET 235 8.5.1 SINGLE DISTANCE BETWEEN THE DONOR AND THE ACCEPTOR 235
8.5.2 DISTRIBUTIONS O F DISTANCES IN DONOR-ACCEPTOR
PAIRS 239
8.5.3 SINGLE MOLECULE STUDIES 242 8.5.4 ON THE VALIDITY O F FORSTER S
THEORY FOR THE ESTIMATION OF DISTANCES 242 8.6 FRET IN ENSEMBLES O F
DONORS AND ACCEPTORS 243
8.6.1 FRET IN THREE DIMENSIONS: EFFECT O F VISCOSITY 243 8.6.2 EFFECTS
OF DIMENSIONALITY ON FRET 247 8.6.3 EFFECTS O F RESTRICTED GEOMETRIES ON
FRET 250 8.7 FRET BETWEEN LIKE MOLECULES: EXCITATION ENERGY MIGRATION IN
ASSEMBLIES O F CHROMOPHORES 250 8.7.1 FRET WITHIN A PAIR O F LIKE
CHROMOPHORES 251 8.7.2 FRET IN ASSEMBLIES O F LIKE CHROMOPHORES 251
8.7.3 LACK O F ENERGY TRANSFER UPON EXCITATION AT THE RED EDGE OF THE
ABSORPTION SPECTRUM (WEBER S RED-EDGE EFFECT) 252 8.8 OVERVIEW O F
QUALITATIVE AND QUANTITATIVE APPLICATIONS O F FRET 252 BIBLIOGRAPHY 258
PART II TECHNIQUES 263
9 STEADY-STATE SPECTROFLUOROMETRY 265 9.1 OPERATING PRINCIPLES O F A
SPECTROFLUOROMETER 265 9.2 CORRECTION OF EXCITATION SPECTRA 268
IMAGE 6
X | CONTENTS
9.3 CORRECTION OF EMISSION SPECTRA 268
9.4 MEASUREMENT O F FLUORESCENCE QUANTUM YIELDS 269 9.5 POSSIBLE
ARTIFACTS IN SPECTROFLUOROMETRY 271 9.5.1 INNER FILTER EFFECTS 271
9.5.1.1 EXCITATION INNER FILTER EFFECT 271
9.5.1.2 EMISSION INNER FILTER EFFECT (SELF-ABSORPTION) 272 9.5.1.3 INNER
FILTER EFFECTS DUE TO THE PRESENCE O F OTHER SUBSTANCES 274 9.5.2
AUTOFLUORESCENCE 274 9.5.3 POLARIZATION EFFECTS 275 9.5.4 EFFECT O F
OXYGEN 275 9.5.5 PHOTOBLEACHING EFFECT 276 9.6 MEASUREMENT O F
STEADY-STATE EMISSION ANISOTROPY:
POLARIZATION SPECTRA 277 9.6.1 PRINCIPLES OF MEASUREMENT 277 9.6.2
POSSIBLE ARTIFACTS 279 9.6.3 TESTS PRIOR TO FLUORESCENCE POLARIZATION
MEASUREMENTS 279 APPENDIX 9.A ELIMINATION O F POLARIZATION EFFECTS IN
THE MEASUREMENT OF
FLUORESCENCE INTENSITY 281 BIBLIOGRAPHY 283
10 TIME-RESOLVED FLUORESCENCE TECHNIQUES 285 10.1 BASIC EQUATIONS OF
PULSE AND PHASE-MODULATION FLUORIMETRIES 286 10.1.1 PULSE FLUORIMETRY
286 10.1.2 PHASE-MODULATION FLUORIMETRY 286
10.1.3 RELATIONSHIP BETWEEN HARMONIC RESPONSE AND 8-PULSE RESPONSE 287
10.1.4 GENERAL RELATIONS FOR SINGLE EXPONENTIAL AND MULTIEXPONENTIAL
DECAYS 290 10.2 PULSE FLUORIMETRY 292
10.2.1 LIGHT SOURCES 292 10.2.2 SINGLE-PHOTON TIMING TECHNIQUE (10
PS-500 US) 292 10.2.3 STREAK CAMERA (IPS-LONS) 294 10.2.4 FLUORESCENCE
UPCONVERSION (0.1-500PS) 295 10.2.5 OPTICAL KERR-GATING (0.1-500PS) 297
10.3 PHASE-MODULATION FLUORIMETRY 298
10.3.1 INTRODUCTION 298 10.3.2 PHASE FLUORIMETERS USING A CONTINUOUS
LIGHT SOURCE AND AN ELECTRO-OPTIC MODULATOR 300 10.3.3 PHASE
FLUORIMETERS USING THE HARMONIC CONTENT O F A PULSED
LASER 302
10.4 ARTIFACTS IN TIME-RESOLVED FLUORIMETRY 302 10.4.1 INNER FILTER
EFFECTS 302 10.4.2 DEPENDENCE O F THE INSTRUMENT RESPONSE ON
WAVELENGTH-COLOR EFFECT 304
IMAGE 7
CONTENTS | X I
10.4.3 POLARIZATION EFFECTS 304
10.4.4 EFFECTS O F LIGHT SCATTERING 304 10.5 DATA ANALYSIS 305 10.5.1
PULSE FLUORIMETRY 305
10.5.2 PHASE-MODULATION FLUORIMETRY 306 10.5.3 JUDGING THE QUALITY O F
THE FIT 306 10.5.4 GLOBAL ANALYSIS 307 10.5.5 FLUORESCENCE DECAYS WITH
UNDERLYING DISTRIBUTIONS O F DECAY
TIMES 308
10.6 LIFETIME STANDARDS 312 10.7 TIME-RESOLVED POLARIZATION MEASUREMENTS
314 10.7.1 GENERAL EQUATIONS FOR TIME-DEPENDENT ANISOTROPY AND POLARIZED
COMPONENTS 314
10.7.2 PULSE FLUORIMETRY 315 10.7.3 PHASE-MODULATION FLUORIMETRY 317
10.7.4 REFERENCE COMPOUNDS FOR TIME-RESOLVED FLUORESCENCE ANISOTROPY
MEASUREMENTS 318
10.8 TIME-RESOLVED FLUORESCENCE SPECTRA 318 10.9 LIFETIME-BASED
DECOMPOSITION O F SPECTRA 318 10.10 COMPARISON BETWEEN SINGLE-PHOTON
TIMING FLUORIMETRY AND PHASE-MODULATION FLUORIMETRY 322
BIBLIOGRAPHY 323
11 FLUORESCENCE MICROSCOPY 327 11.1 WIDE-FIELD (CONVENTIONAL), CONFOCAL,
AND TWO-PHOTON FLUORESCENCE MICROSCOPIES 328 11.1.1 WIDE-FIELD
(CONVENTIONAL) FLUORESCENCE
MICROSCOPY 328 11.1.2 CONFOCAL FLUORESCENCE MICROSCOPY 329 11.1.3
TWO-PHOTON EXCITATION FLUORESCENCE MICROSCOPY 331 11.1.4 FLUORESCENCE
POLARIZATION MEASUREMENTS IN MICROSCOPY 333 11.2 SUPER-RESOLUTION
(SUBDIFFRACTION) TECHNIQUES 333
11.2.1 SCANNING NEAR-FIELD OPTICAL MICROSCOPY (SNOM) 333 11.2.2
FAR-FIELD TECHNIQUES 337 11.3 FLUORESCENCE LIFETIME IMAGING MICROSCOPY
(FLIM) 340 11.3.1 TIME-DOMAIN FLIM 341
11.3.2 FREQUENCY-DOMAIN FLIM 342 11.4 APPLICATIONS 342 BIBLIOGRAPHY 346
12 FLUORESCENCE CORRELATION SPECTROSCOPY AND SINGLE-MOLECULE
FLUORESCENCE SPECTROSCOPY 349 12.1 FLUORESCENCE CORRELATION SPECTROSCOPY
(FCS) 349 12.1.1 CONCEPTUAL BASIS AND INSTRUMENTATION 350
12.1.2 DETERMINATION O F TRANSLATIONAL DIFFUSION COEFFICIENTS 355
IMAGE 8
X I I I CONTENTS
12.1.3 CHEMICAL KINETIC STUDIES 356
12.1.4 DETERMINATION OF ROTATIONAL DIFFUSION COEFFICIENTS 359 12.1.5
CROSS-CORRELATION METHODS 360 12.2 SINGLE-MOLECULE FLUORESCENCE
SPECTROSCOPY 360 12.2.1 GENERAL REMARKS 360
12.2.2 SINGLE-MOLECULE DETECTION IN FLOWING SOLUTIONS 361 12.2.3
SINGLE-MOLECULE DETECTION USING FLUORESCENCE MICROSCOPY TECHNIQUES 363
12.2.4 SINGLE-MOLECULE AND SINGLE-PARTICLE PHOTOPHYSICS 367 12.2.5
APPLICATIONS AND USEFULNESS O F SINGLE-MOLECULE
FLUORESCENCE 371
BIBLIOGRAPHY 372
PART III APPLICATIONS 377
13 EVALUATION OF LOCAL PHYSICAL PARAMETERS BY MEANS OF FLUORESCENT
PROBES 379 13.1 FLUORESCENT PROBES FOR POLARITY 379 13.1.1 EXAMPLES O F
PHOTOINDUCED CHARGE TRANSFER (PCT) PROBES FOR
POLARITY 380
13.1.2 PYRENE AND ITS DERIVATIVES 384 13.2 ESTIMATION OF
MICROVISCOSITY, FLUIDITY, AND MOLECULAR MOBILITY 384 13.2.1 VARIOUS
METHODS 385 13.2.2 USE OF MOLECULAR ROTORS 386
13.2.3 METHODS BASED ON INTERMOLECULAR QUENCHING OR INTERMOLECULAR
EXCIMER FORMATION 389 13.2.4 METHODS BASED ON INTRAMOLECULAR EXCIMER
FORMATION 390 13.2.5 FLUORESCENCE POLARIZATION METHOD 393
13.2.5.1 CHOICE O F PROBES 393 13.2.5.2 HOMOGENEOUS ISOTROPIC MEDIA 393
13.2.5.3 ORDERED SYSTEMS 395 13.2.5.4 PRACTICAL ASPECTS 395 13.2.6
CONCLUDING REMARKS 397 13.3 TEMPERATURE 398 13.4 PRESSURE 402
BIBLIOGRAPHY 404
14 CHEMICAL SENSING VIA FLUORESCENCE 409 14.1 INTRODUCTION 409 14.2
VARIOUS APPROACHES O F FLUORESCENCE SENSING 410 14.3 FLUORESCENT P H
INDICATORS 412
14.3.1 PRINCIPLES 412 14.3.2 THE MAIN FLUORESCENT PH INDICATORS 417
14.3.2.1 COUMARINS 417
IMAGE 9
CONTENTS X I I I
14.3.2.2 PYRANINE 417
14.3.2.3 FLUORESCEIN AND ITS DERIVATIVES 419 14.3.2.4 SNARF AND SNAFL
419 14.3.2.5 PH INDICATORS BASED ON PHOTOINDUCED ELECTRON TRANSFER (PET)
420 14.4 DESIGN PRINCIPLES O F FLUORESCENT MOLECULAR SENSORS BASED ON
ION OR
MOLECULE RECOGNITION 420 14.4.1 GENERAL ASPECTS 420 14.4.2 RECOGNITION
UNITS AND TOPOLOGY 422 14.4.3 PHOTOPHYSICAL SIGNAL TRANSDUCTION 424
14.4.3.1 PHOTOINDUCED ELECTRON TRANSFER (PET) 424 14.4.3.2 PHOTOINDUCED
CHARGE TRANSFER (PCT) 425 14.4.3.3 EXCIMER FORMATION OR DISAPPEARANCE
427 14.4.3.4 FORSTER RESONANCE ENERGY TRANSFER
(FRET) 427
14.5 FLUORESCENT MOLECULAR SENSORS O F METAL IONS 427 14.5.1 GENERAL
ASPECTS 427 14.5.2 FLUORESCENT PET CATION SENSORS 430 14.5.3 FLUORESCENT
PCT CATION SENSORS 430 14.5.4 EXCIMER-BASED CATION SENSORS 430
14.5.5 CATION SENSORS BASED ON FRET 430 14.5.6 HYDROXYQUINOLINE-BASED
CATION SENSORS 432 14.5.7 CONCLUDING REMARKS ON CATION SENSORS 435 14.6
FLUORESCENT MOLECULAR SENSORS O F ANIONS 436
14.6.1 ANION SENSORS BASED ON COLLISIONAL QUENCHING 437 14.6.2 ANION
SENSORS BASED ON FLUORESCENCE CHANGES UPON ANION BINDING 437 14.6.2.1
UREA AND THIOUREA GROUPS 438
14.6.2.2 PYRROLE GROUPS 439 14.6.2.3 POLYAZAALKANES 440 14.6.2.4
IMIDAZOLIUM GROUPS 443 14.6.2.5 ANION BINDING BY METAL ION COMPLEXES 443
14.6.3 ANION SENSORS BASED ON THE DISPLACEMENT O F A COMPETITIVE
FLUORESCENT ANIONIC MOLECULE 444 14.7 FLUORESCENT MOLECULAR SENSORS OF
NEUTRAL MOLECULES 445 14.7.1 CYCLODEXTRIN-BASED FLUORESCENT SENSORS 446
14.7.2 BORONIC ACID-BASED FLUORESCENT SENSORS 449
14.7.3 PORPHYRIN-BASED FLUORESCENT SENSORS 452 14.8 FLUORESCENCE SENSING
O F GASES 453 14.8.1 OXYGEN 453 14.8.2 CARBON DIOXIDE 456
14.8.3 NITRIC OXIDE 456 14.8.4 EXPLOSIVES 456 14.9 SENSING DEVICES 458
14.10 REMOTE SENSING BY FLUORESCENCE LIDAR 460
IMAGE 10
X I V | CONTENTS
14.10.1 VEGETATION MONITORING 461
14.10.2 MARINE MONITORING 462 14.10.3 HISTORIC MONUMENTS 462 APPENDIX
14.A. SPECTROPHOTOMETRIC AND SPECTROFLUOROMETRIC PH TITRATIONS 462
SINGLE-WAVELENGTH MEASUREMENTS 462
DUAL-WAVELENGTH MEASUREMENTS 463 APPENDIX 14.B. DETERMINATION OF THE
STOICHIOMETRY AND STABILITY CONSTANT OF METAL COMPLEXES FROM
SPECTROPHOTOMETRIC OR SPECTROFLUOROMETRIC TITRATIONS 465
DEFINITION OF THE EQUILIBRIUM CONSTANTS 465 PRELIMINARY REMARKS ON
TITRATIONS BY SPECTROPHOTOMETRY AND SPECTROFLUOROMETRY 467
FORMATION OF A 1:1 COMPLEX (SINGLE-WAVELENGTH MEASUREMENTS) 467
FORMATION O F A 1:1 COMPLEX (DUAL-WAVELENGTH MEASUREMENTS) 469 FORMATION
O F SUCCESSIVE COMPLEXES ML AND M 2 L 470 COOPERATIVITY 471
DETERMINATION O F THE STOICHIOMETRY O F A COMPLEX BY THE METHOD OF
CONTINUOUS VARIATIONS (JOB S METHOD) 471 BIBLIOGRAPHY 473
15 AUTOFLUORESCENCE AND FLUORESCENCE LABELING IN BIOLOGY AND MEDICINE
479 15.1 INTRODUCTION 479 15.2 NATURAL (INTRINSIC) CHROMOPHORES AND
FLUOROPHORES 480 15.2.1 AMINO ACIDS AND DERIVATIVES 481
15.2.2 COENZYMES 488 15.2.3 CHLOROPHYLLS 490 15.3 FLUORESCENT PROTEINS
(FPS) 491 15.4 FLUORESCENT SMALL MOLECULES 493 15.5 QUANTUM DOTS AND
OTHER LUMINESCENT NANOPARTICLES 497 15.6 CONCLUSION 501
BIBLIOGRAPHY 502
16 MISCELLANEOUS APPLICATIONS 507 16.1 FLUORESCENT WHITENING AGENTS 507
16.2 FLUORESCENT NONDESTRUCTIVE TESTING 508 16.3 FOOD SCIENCE 511 16.4
FORENSICS 513 16.5 COUNTERFEIT DETECTION 514
16.6 FLUORESCENCE IN ART 515 BIBLIOGRAPHY 518
APPENDIX: CHARACTERISTICS O F FLUORESCENT ORGANIC COMPOUNDS 521 EPILOGUE
551 INDEX 553
|
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| author | Valeur, Bernard Berberan-Santos, Mário Nuno |
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| building | Verbundindex |
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| ctrlnum | (OCoLC)794259100 (DE-599)DNB1016558465 |
| dewey-full | 543.08584 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 543 - Analytical chemistry |
| dewey-raw | 543.08584 |
| dewey-search | 543.08584 |
| dewey-sort | 3543.08584 |
| dewey-tens | 540 - Chemistry and allied sciences |
| discipline | Chemie / Pharmazie Physik Biologie Chemie |
| edition | 2. ed. |
| format | Book |
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| id | DE-604.BV040140453 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T00:17:47Z |
| institution | BVB |
| isbn | 9783527328376 9783527328468 9783527650033 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-024997404 |
| oclc_num | 794259100 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-91G DE-BY-TUM DE-188 DE-20 DE-703 DE-19 DE-BY-UBM DE-11 DE-634 DE-29T DE-29 DE-92 DE-861 |
| owner_facet | DE-355 DE-BY-UBR DE-91G DE-BY-TUM DE-188 DE-20 DE-703 DE-19 DE-BY-UBM DE-11 DE-634 DE-29T DE-29 DE-92 DE-861 |
| physical | XXI, 569 S. Ill., graph. Darst. |
| publishDate | 2012 |
| publishDateSearch | 2012 |
| publishDateSort | 2012 |
| publisher | Wiley-VCH |
| record_format | marc |
| spelling | Valeur, Bernard Verfasser aut Molecular fluorescence principles and applications Bernard Valeur and Mário Nuno Berberan-Santos 2. ed. Weinheim Wiley-VCH 2012 XXI, 569 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Fluoreszenzspektroskopie (DE-588)4017701-4 gnd rswk-swf Fluoreszenzspektroskopie (DE-588)4017701-4 s DE-604 Berberan-Santos, Mário Nuno Verfasser aut DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024997404&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Valeur, Bernard Berberan-Santos, Mário Nuno Molecular fluorescence principles and applications Fluoreszenzspektroskopie (DE-588)4017701-4 gnd |
| subject_GND | (DE-588)4017701-4 |
| title | Molecular fluorescence principles and applications |
| title_auth | Molecular fluorescence principles and applications |
| title_exact_search | Molecular fluorescence principles and applications |
| title_full | Molecular fluorescence principles and applications Bernard Valeur and Mário Nuno Berberan-Santos |
| title_fullStr | Molecular fluorescence principles and applications Bernard Valeur and Mário Nuno Berberan-Santos |
| title_full_unstemmed | Molecular fluorescence principles and applications Bernard Valeur and Mário Nuno Berberan-Santos |
| title_short | Molecular fluorescence |
| title_sort | molecular fluorescence principles and applications |
| title_sub | principles and applications |
| topic | Fluoreszenzspektroskopie (DE-588)4017701-4 gnd |
| topic_facet | Fluoreszenzspektroskopie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024997404&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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