Quantitative methods in proteomics:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Springer
2012
|
| Schriftenreihe: | Methods in molecular biology
893 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XV, 539 S. Ill., graph. Darst. |
| ISBN: | 9781617798849 |
Internformat
MARC
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| 245 | 1 | 0 | |a Quantitative methods in proteomics |c ed. by Katrin Marcus |
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Datensatz im Suchindex
| _version_ | 1804149085322608640 |
|---|---|
| adam_text | Titel: Quantitative methods in proteomics
Autor: Marcus, Katrin
Jahr: 2012
Contents
Preface.......................................................... v
Contributors...................................................... xi
Part I Planning a Proteomic Experiment
1 Important Issues in Planning a Proteomics Experiment: Statistical
Considerations of Quantitative Proteomic Data........................ 3
Katharina Podwojski, Christian Stephan, and Martin Eisenocker
Part II Quantitative Proteomics: Gel-Based Methods
2 The Whereabouts of 2D Gels in Quantitative Proteomics................ 25
Thierry Rabilloud
3 Proteome Analysis with Classical 2D-PAGE.......................... 37
Caroline May, Frederic Brosseron, Kathy Pfeiffer, Helmut E. Meyer,
and Katrin Marcus
4 Fast and Sensitive Coomassie Staining in Quantitative Proteomics.......... 47
Nadine Dyballa and Sabine Metzger
5 Silver Staining of 2D Electrophoresis Gels............................ 61
Thierry Rabilloud
6 Differential Proteome Analysis Using 2D-DIGE....................... 75
Caroline May, Frederic Brosseron, Piotr Chartowski, Helmut E. Meyer,
and Katrin Marcus
Part III Quantitative Proteomics: MS-Based Methods
7 Quantitative Mass Spectrometry-Based Proteomics: An Overview.......... 85
Miroslav Nikolov, Carla Schmidt, and Henning Urlaub
8 Robust Workflow for iTRAQ-Based Peptide and Protein Quantification..... 101
Florian Beck, Julia Maria Burkhart, Joerg Geiger, René Peiman Zahedi,
and Albert Sickmann
9 Relative Protein Quantification by MS/MS Using the
Tandem Mass Tag Technology.................................... 115
Loïc Dayon and Jean-Charles Sanchez
10 A Rapid Approach for Isobaric Peptide Termini Labeling................ 129
Christian J. Koehler, Magnus 0. Arntzen, Achim Treumann,
and Bernd Thiede
11 Isotope-Coded Protein Label..................................... 143
Josef Kellermann and Friedrich Lottspeich
VII
viii Contents
12 Hydroponic Isotope Labeling of Entire Plants and High-Performance
Mass Spectrometry for Quantitative Plant Proteomics................... 155
Laurence V. Bindschedler, DaviniaJ.S. Mills, and Rainer Cramer
13 In Vivo Quantitative Proteome Profiling: Planning and Evaluation of SILAC
Experiments.................................................. 175
Marieluise Kirchner and Matthias Setback
14 SILAC for the Study of Mammalian Cell Lines and Yeast Protein Complexes. . 201
Heike Piechura, Silke Oeljeklaus, and Bettina Warscheid
15 Post-digestion lsO Exchange/Labeling for Quantitative Shotgun Proteomics of
Membrane Proteins............................................. 223
Xiaoying Te, Brian T. Luke, Donald J. Johann Jr, King C. Chan,
DaRue A. Prieto, Akira Ono, Timothy D. Veenstra, andjosip Blonder
16 Application of Label-Free Proteomics for Differential Analysis
of Lung Carcinoma Cell Line A549................................ 241
Barbara Sitek, Daniel M. Waldera-Lupa, Gereon Poschmann,
Helmut E. Meyer, and Kai Stühler
17 Absolute Quantification of Proteins Using Standard Peptides
and Multiple Reaction Monitoring................................. 249
Carla Schmidt and Henning Urlaub
18 Absolute Multiplexed Protein Quantification Using QconCAT Technology. . . 267
Philip J. Brownridge, Victoria M. Harman, Deborah M. Simpson,
and Robert J. Beynon
19 A Practical Guide to the FLEXIQuant Method........................ 295
Sasha Singh, Marc Kirchner, Judith A. Steen, and Hanno Steen
20 Label-Free Protein Quantitation Using Weighted Spectral Counting........ 321
Christine Vogel and Edward M. Marcotte
Part IV Specific Methods - Detection and Quantification
op Post-Translational Modifications
21 Discovering the Phosphoproteome of the Hydrophobic Cytochrome c Oxidase
Membrane Protein Complex...................................... 345
Stefan Helling, Maik Hüttemann, Bernhard Kadenbach, Rabia Ramzan,
Sebastian Vogt, and Katrin Marcus
22 KiC Assay: A Quantitative Mass Spectrometry-Based Approach............ 359
Tadong Huang and Jay J. Thelen
23 Robust and High-Throughput Sample Preparation for (Semi-)Quantitative
Analysis of N-Glycosylation Profiles from Plasma Samples................ 371
L. Renee Ruhaak, Carolin Huhn, Carolien A.M. Koeleman,
André M. Deelder, and Manfred Wahrer
24 Quantitative Redox Proteomics: The NOxICAT Method................ 387
Claudia Lindemann and Lars I. Leichert
25 Quantitative Analysis of S-Nitrosylated Proteins....................... 405
Federico Torta and Angela Bachi
Contents ix
26 Analysis of Ubiquitinated Proteome by Quantitative Mass Spectrometry..... 417
Chan Hyun Na andjunmin Peng
27 Identification of Endogenous SUMOl Accepter Sites by Mass Spectrometry. . 431
He-Hsuan Hsiao, Erik Meulmeester, and Henning Urlaub
Part V Data Analysis and Software Tools
28 Search and Decoy: The Automatic Identification of Mass Spectra.......... 445
Martin Eisenacher, Michael Kohl, Michael Turewicz, Markus-Hermann Koch,
Julian Uszkoreit, and Christian Stephan
29 Software Tools for MS-Based Quantitative Proteomics: A Brief Overview .... 489
Simone Lemeer, Hannes Hahne, Fiona Pacht, and Bernhard Küster
30 îTRAQ Data Interpretation....................................... 501
Marc Vaudel, Julia Maria Burkhart, René Peiman Zahedi, Lennart Martens,
and Albert Sickmann
31 MSQuant: A Platform for Stable Isotope-Based Quantitative Proteomics..... 511
Joost W. Gouw andjeroen Krijgsveld
Index............................................................... 523
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| isbn | 9781617798849 |
| language | English |
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| physical | XV, 539 S. Ill., graph. Darst. |
| publishDate | 2012 |
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| publisher | Springer |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Quantitative methods in proteomics ed. by Katrin Marcus New York [u.a.] Springer 2012 XV, 539 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 893 Springer protocols Methode (DE-588)4038971-6 gnd rswk-swf Proteomanalyse (DE-588)4596545-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Proteomanalyse (DE-588)4596545-6 s Methode (DE-588)4038971-6 s b DE-604 Marcus, Katrin 1972- Sonstige (DE-588)173564089 oth Methods in molecular biology 893 (DE-604)BV035362695 893 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024980278&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Quantitative methods in proteomics Methods in molecular biology Methode (DE-588)4038971-6 gnd Proteomanalyse (DE-588)4596545-6 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4596545-6 (DE-588)4143413-4 |
| title | Quantitative methods in proteomics |
| title_auth | Quantitative methods in proteomics |
| title_exact_search | Quantitative methods in proteomics |
| title_full | Quantitative methods in proteomics ed. by Katrin Marcus |
| title_fullStr | Quantitative methods in proteomics ed. by Katrin Marcus |
| title_full_unstemmed | Quantitative methods in proteomics ed. by Katrin Marcus |
| title_short | Quantitative methods in proteomics |
| title_sort | quantitative methods in proteomics |
| topic | Methode (DE-588)4038971-6 gnd Proteomanalyse (DE-588)4596545-6 gnd |
| topic_facet | Methode Proteomanalyse Aufsatzsammlung |
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