Host-fungus interactions: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Humana Press
2012
|
| Schriftenreihe: | Methods in molecular biology
845 |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVI, 597 S. Ill., graph. Darst. |
| ISBN: | 9781617795381 |
Internformat
MARC
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| 245 | 1 | 0 | |a Host-fungus interactions |b methods and protocols |c ed. by Alexandra C. Brand ; Donna M. MacCallum |
| 264 | 1 | |a New York [u.a.] |b Humana Press |c 2012 | |
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Datensatz im Suchindex
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| adam_text | Host-Fungus Interactions
Methods and Protocols
Edited by
Alexandra C Brand
School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen,
Foresterhill, Aberdeen, UK
Donna M MacCallum
School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen,
Foresterhill, Aberdeen, UK
Humana Press
Contents
Preface v
Contributors xi
PART I GENE DISRUPTION
1 Gene Deletion in Candida albicans Wild-Type Strains
Using the S^477-Flipping Strategy 3
Christoph Sasse and Joachim Morschhauser
2 Mini-blaster-Mediated Targeted Gene Disruption and Marker
Complementation in Candida albicans 19
Shantanu Ganguly and Aaron P Mitchell
3 Rapid Detection of Aneuploidy Following the Generation
of Mutants in Candida albicans 41
Megan D Lenardon and Andre Nantel
4 Agrobacterium-Mediated Insertional Mutagenesis
in Histoplasma capsulatum 51
Olga Zemska and Chad A Rappleye
5 Targeted Gene Disruption in Cryptococcus neoformans Using
Double-Joint PCR with Split Dominant Selectable Markers 67
Min Su Kim, Seo-Young Kim, Kwang-Woo Jung,
and Yong-Sun Balm
6 Multiple Gene Deletion in Cryptococcus neoformans Using
the Crc-lox System 85
Lorina G Baker and Jennifer K Lodge
7 Gene Disruption in Aspergillus fumigatus Using a PCR-Based
Strategy and In Vivo Recombination in Yeast 99
Iran Malavazi and Gustavo Henrique Goldman
8 Targeted Gene Deletion in Aspergillus fumigatus Using
the Hygromycin-Resistance Split-Marker Approach 119
Fabrice N Gravelat, David S Askew, and Donald C Sheppard
9 Gene Disruption in Coccidioides Using Hygromycin
or Phleomycin Resistance Markers 131
Chiung-Yu Hung, Hua Zhang Wise, and Garry T Cole
PART II MODULATION OF GENE EXPRESSION: RNAI GENE KNOCKDOWN
10 RNAi-Based Gene Silencing Using a GFP Sentinel System
in Histoplasma capsulatum 151
Brian H, Yousejf and Chad A Rappleye
vii
viii Contents
11 RNA Interference in Cryptococcus neoformans 165
Michael L Skowyra and Tamara L Doering
12 Gene Knockdown in Paracoccidioides brasiliensis
Using Antisense RNA 187
Jodo F Menino, Agostinho J Almeida, and Fernando Rodrigues
PART III MODULATION OF GENE EXPRESSION: REGULATABLE PROMOTERS
13 Tetracycline-Inducible Gene Expression in Candida albicans 201
Michael Weyler and Joachim Morschhauser
14 Galactose-Inducible Promoters in Cryptococcus neoformans var grubii 211
Lorina G Baker and Jennifer K Lodge
15 Modular Gene Over-expression Strategies for Candida albicans 227
Vitor Cabral, Murielle Chauvel, Arnaud Firon, Melanie Legrand,
Audrey Nesseir, Sophie Bachellier-Bassi, Yogesh Chaudhari,
Carol A Munro, and Christophe d Enfert
PART IV HOST RESPONSES TO INFECTION IN VITRO
16 Interactions Between Macrophages and Cell Wall Oligosaccharides
of Candida albicans 247
Hector M Mora-Montes, Christopher McKenzie, Judith M Bain,
Leanne E Lewis, Lars P Erwig, and Neil A R Gow
17 Murine Bone Marrow-Derived Dendritic Cells and T-Cell
Activation by Candida albicans 261
Joanne Gibson, Neil A R Gow, and Simon Y C Wong
18 Phagocytosis and Intracellular Killing of Candida albicans
by Murine Polymorphonuclear Neutrophils 277
Alieke G Vonk, Mihai G Netea, and Bart Jan Kullberg
19 Human Oral Keratinocytes: A Model System to Analyze
Host-Pathogen Interactions 289
Torsten Wollert, Christiane Rollenhagen, George M Langford,
and Paula Sundstrom
20 Simple Assays for Measuring Innate Interactions with Fungi 303
Ann M Kerrigan, Maria da Gloria Teixeira de Sousa,
and Gordon D Brown
21 Binding and Uptake of Candida, albicans by Human
Monocyte-Derived Dendritic Cells 319
Annemiek B van Spriel and Alessandra Cambi
22 Immune Responses to Candida albicans in Models of In Vitro
Reconstituted Human Oral Epithelium 333
Jeanette Wagener, Daniela Mailander-Sanchez, and Martin Schaller
23 Analysis of Host-Cell Responses by Immunoblotting, ELISA,
and Real-Time PCR 345
David L Moyes and Julian R Naglik
Contents ix
24 In Vitro Model of Invasive Pulmonary Aspergillosis
in the Human Alveolus 361
Lea Gregson, William W Hope, and Susan J Howard
25 Biofilm Formation Studies in Microtiter Plate Format 369
Marta Riera, Emilia Moreno-Ruiz, Sophie Goyard,
Christophe d Enfert, and Guilhem Janbon
PART V FUNGAL RESPONSES DURING INFECTION
26 Transcript Profiling Using ESTs from Paracoccidioides brasiliensis
in Models of Infection 381
Alexandre Melo Bailao, Maristela Pereira,
Silvia Maria Salem-Izacc, Clayton Luiz Borges,
and Celia Maria de Almeida Soares
27 Laser Capture Microdissection of Candida albicans from Host Tissue 397
Caroline Westwater and David A Schofield
28 Isolation and Amplification of Fungal RNA for Microarray Analysis
from Host Samples 411
Anja Luttich, Sascha Brunke, and Bernhard Hube
PART VI HOST RESPONSES TO INFECTION IN VIVO
29 Cytokine Measurement Using Cytometric Bead Arrays 425
Luis Castillo and Donna M MacCallum
30 Transcript Profiling of the Murine Immune Response
to Invasive Aspergillosis 435
Zaneeta Dhesi, Susanne Herbst, and Darius Armstrong-James
PART VII NON-MAMMALIAN MODEL SYSTEMS
FOR HOST-FUNGAL INTERACTIONS
31 Caenorhabditis elegans A Nematode Infection Model
for Pathogenic Fungi 447
Maged Muhammed, Jeffrey J Coleman, and Eleftherios Mylonakis
32 Drosophila melanojjaster as a Model Organism for Invasive Aspergillosis 455
Michail S Lionakis and Dimitrios P Kontoyiannis
33 Galleria mellonella as a Model for Fungal Pathogenicity Testing 469
John Fallon, Judy Kelly, and Kevin Kavanagh
34 Embryonated Chicken Eggs as Alternative Infection Model
for Pathogenic Fungi 487
Use D Jacobsen, Katharina Grofie, and Bernhard Hube
PART VIII MAMMALIAN HOSTS AS INFECTION MODELS
35 Mouse Intravenous Challenge Models and Applications
Donna M MacCallum
X Contents
36 A Nebulized Intra-tracheal Rat Model of Invasive
Pulmonary Aspergillosis 511
Guillaume Desoubeaux and Jacques Chandenier
37 Invasive Models of Histoplasmosis 519
A George Smulian
38 Murine Model of Concurrent Oral and Vaginal
Candida albicans Colonisation 527
Durdana Rahman, Mukesh Mistry, Selvam Thavaraj,
Julian R Naglik, and Stephen J Challacombe
39 A Luciferase Reporter for Gene Expression Studies and Dynamic
Imaging of Superficial Candida albicans Infections 537
Donatella Pietrella, Brice Enjalbert, Ute Zeidler, Sadri Znaidi,
Anna Rachini, Anna Vecchiarelli, and Christophe d Enfert
40 Modeling of Fungal Biofilms Using a Rat Central Vein Catheter 547
Jeniel E Nett, Karen Marchillo, and David R Andes
41 Orogastrointestinal Model of Mucosal and Disseminated Candidiasis 557
Karl V Clemons and David A Stevens
42 A Nonlethal Murine Cutaneous Model of Invasive Aspergillosis 569
Ronen Ben-Ami and Dimitrios P Kontoyiannis
Index 583
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| series2 | Methods in molecular biology Springer protocols |
| spelling | Host-fungus interactions methods and protocols ed. by Alexandra C. Brand ; Donna M. MacCallum New York [u.a.] Humana Press 2012 XVI, 597 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 845 Springer protocols Brand, Alexandra C. Sonstige oth Methods in molecular biology 845 (DE-604)BV035362695 845 HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024973599&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Host-fungus interactions methods and protocols Methods in molecular biology |
| title | Host-fungus interactions methods and protocols |
| title_auth | Host-fungus interactions methods and protocols |
| title_exact_search | Host-fungus interactions methods and protocols |
| title_full | Host-fungus interactions methods and protocols ed. by Alexandra C. Brand ; Donna M. MacCallum |
| title_fullStr | Host-fungus interactions methods and protocols ed. by Alexandra C. Brand ; Donna M. MacCallum |
| title_full_unstemmed | Host-fungus interactions methods and protocols ed. by Alexandra C. Brand ; Donna M. MacCallum |
| title_short | Host-fungus interactions |
| title_sort | host fungus interactions methods and protocols |
| title_sub | methods and protocols |
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