Ribozymes: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York, NY [u.a.]
Humana Press
2012
|
| Schriftenreihe: | Methods in molecular biology
848 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIV, 480 S. Ill., graph. Darst. |
| ISBN: | 9781617795442 |
Internformat
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Datensatz im Suchindex
| _version_ | 1804149075198607360 |
|---|---|
| adam_text | Titel: Ribozymes
Autor: Hartig, Jörg Steffen
Jahr: 2012
Contents
Preface.............................................................. v
Contributors.......................................................... xi
1 Introduction.................................................. 1
Eric Westhof
2 Characterization of Hammerhead Ribozyme Reactions.................. 5
Anne Kaiweit, Rita Przybilski, Carsten Seehafer,
Marcos de laPena, and Christian Hammann
3 Mechanistic Analysis of the Hepatitis Delta Virus (HDV) Ribozyme:
Methods for RNA Preparation, Structure Mapping, Solvent Isotope
Effects, and Co-transcriptional Cleavage............................. 21
Durga M. Chadalavada, Andrea L. Cerrone-Szakal,
Jennifer L. Wilcox, Nathan A. Siegfried, and Philip C. Bevilacqua
4 Kinetic Characterization of Hairpin Ribozyme Variants.................. 41
Bettina Appel, Thomas Marschall, Anne Strahl,
and Sabine Müller
5 Characterization of RNase P RNA Activity........................... 61
Markus Gößrinjjer, Dominik Helmecke,
and Roland K. Hartmann
6 Group I Intron Ribozymes....................................... 73
Henrik Nielsen
7 Kinetic Characterization of Group II Intron Folding and Splicing.......... 91
Olga Fedorova
8 Mechanism and Distribution of ßlmS Ribozymes....................... 113
Phillip J. McCown, Wade C. Winkler, and Ronald R. Breaker
9 Structure-Based Search and In Vitro Analysis
of Self-Cleaving Ribozymes....................................... 131
Randi M. Jimenez and Andrej Luptdk
10 Discovery of RNA Motifs Using a Computational Pipeline
that Allows Insertions in Paired Regions and Filtering
of Candidate Sequences......................................... 145
Randi M. Jimenez, Ladislav Rampdfck, Broha Brejovd,
Tomdi Vinaf, and Andrej Luptdk
11 Crystallographic Analysis of Small Ribozymes and Riboswitches........... 159
Geoffrey M. Lippa, Joseph A. Liberman, Jermaine L. Jenkins,
Jolanta Krucinska, Mohammad Salim, and Joseph E. Wedekind
VII
viii Contents
12 Functional Dynamics of RNA Ribozymes Studied
by NMR Spectroscopy.......................................... 185
Boris Pürtiß, Janina Buck, Christian Richter,
and Harald Schwalbe
13 Deoxyribozyme-Based, Semisynthetic Access to Stable
Peptidyl-tRNAs Exemplified by tRNAVal Carrying a Macrolide
Antibiotic Resistance Peptide..................................... 201
Dagmar Graber, Krista Trappl, Jessica Steger,
Anna-Skrollan Geiermann, Lukas Rigger, Holger Moroder,
Norbert Polacek, and Ronald Micura
14 Probing Functions of the Ribosomal Peptidyl Transferase Center
by Nucleotide Analog Interference................................. 215
Matthias D. Er lâcher and Norbert Polacek
15 Single Molecule FRET Characterization of Large Ribozyme Folding........ 227
Lucia Cardo, Krishanthi S. Karunatilaka, David Rueda,
and Roland K.O. Sigel
16 Metal Ion-RNA Interactions Studied via Multinuclear NMR.............. 253
Daniela Donghi and Roland K.O. Sigel
17 Analysis of Catalytic RNA Structure and Function by Nucleotide
Analog Interference Mapping..................................... 275
Soumitra Basu, Mark J. Morris, and Catherine Pazsint
18 In Vitro Selection of Metal Ion-Selective DNAzymes................... 297
Hannah E. Ihms and Yi Lu
19 Selecting Allosteric Ribozymes.................................... 317
Nicolas Piganeau
20 Screening Effective Target Sites on mRNA:
A Ribozyme Library Approach.................................... 329
HoshangJ. Unwalla and John J. Rossi
21 A Computational Approach to Predict Suitable Target Sites
for trans-Acting Minimal Hammerhead Ribozymes..................... 337
Alberto Mercatanti, Caterina Lande, and Lorenzo Citti
22 Targeting mRNAs by Engineered Sequence-Specific
RNase P Ribozymes............................................ 357
Tong Bai, Naresh Sunkara, and Fenyong Liu
23 Target-Induced SOFA-HDV Ribozyme............................. 369
Michel V. Lévesque and Jean-Pierre Perreault
24 Ribozyme-Mediated Trans Insertion-Splicing into Target RNAs........... 385
P. Patrick Dotson II, Jonathan Hart, Christopher Noe,
and Stephen M. Testa
25 Developing Fluorogenic RNA-Cleaving DNAzymes
for Biosensing Applications....................................... 395
M. MonsurAli, Sergio D. Aguirre, Wendy W.K. Mok,
and TCingfu Li
Contents ix
26 Development of Trainable Deoxyribozyme-Based Game
Playing Automaton............................................. 419
Renjun Pet, Joanne Macdonald, and Milan N. Stojanovic
27 Rational Design and Tuning of Ribozyme-Based Devices................ 439
Joe C. Liang and Christina D. Smolke
28 In Vivo Screening of Ligand-Dependent Hammerhead Ribozymes......... 455
Athanasios Saragliadis, Benedikt Klauser, and Jörg S. Hartig
29 Flexizymes as a tRNA Acylation Tool Facilitating Genetic
Code Reprogramming.......................................... 465
Yuki Goto and Hiroaki Suga
Index........................................................... 479
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| indexdate | 2024-07-10T00:17:15Z |
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| isbn | 9781617795442 |
| language | English |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Ribozymes methods and protocols ed. by Jörg S. Hartig New York, NY [u.a.] Humana Press 2012 XIV, 480 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 848 Springer protocols Ribozym (DE-588)4356469-0 gnd rswk-swf Ribozym (DE-588)4356469-0 s DE-604 Hartig, Jörg Steffen 1974- Sonstige (DE-588)125002467 oth Methods in molecular biology 848 (DE-604)BV035362695 848 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024973590&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Ribozymes methods and protocols Methods in molecular biology Ribozym (DE-588)4356469-0 gnd |
| subject_GND | (DE-588)4356469-0 |
| title | Ribozymes methods and protocols |
| title_auth | Ribozymes methods and protocols |
| title_exact_search | Ribozymes methods and protocols |
| title_full | Ribozymes methods and protocols ed. by Jörg S. Hartig |
| title_fullStr | Ribozymes methods and protocols ed. by Jörg S. Hartig |
| title_full_unstemmed | Ribozymes methods and protocols ed. by Jörg S. Hartig |
| title_short | Ribozymes |
| title_sort | ribozymes methods and protocols |
| title_sub | methods and protocols |
| topic | Ribozym (DE-588)4356469-0 gnd |
| topic_facet | Ribozym |
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