Interaktionsmuster von Gliomzellen, Gliomendothelzellen und humanen mesenchymalen Stammzellen:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | German |
| Veröffentlicht: |
2012
|
| Schlagworte: | |
| Online-Zugang: | https://nbn-resolving.org/urn:nbn:de:bvb:19-139348 Volltext Inhaltsverzeichnis |
| Beschreibung: | 115 S. Ill., graph. Darst. |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV040099668 | ||
| 003 | DE-604 | ||
| 005 | 20120523 | ||
| 007 | t | ||
| 008 | 120413s2012 ad|| m||| 00||| ger d | ||
| 016 | 7 | |a 1020531398 |2 DE-101 | |
| 035 | |a (OCoLC)794529101 | ||
| 035 | |a (DE-599)DNB1020531398 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a ger | |
| 049 | |a DE-384 |a DE-473 |a DE-703 |a DE-1051 |a DE-824 |a DE-29 |a DE-12 |a DE-91 |a DE-19 |a DE-1049 |a DE-92 |a DE-739 |a DE-898 |a DE-355 |a DE-706 |a DE-20 |a DE-1102 | ||
| 100 | 1 | |a Korte, Benjamin |e Verfasser |0 (DE-588)1020531258 |4 aut | |
| 245 | 1 | 0 | |a Interaktionsmuster von Gliomzellen, Gliomendothelzellen und humanen mesenchymalen Stammzellen |c von Benjamin Korte |
| 264 | 1 | |c 2012 | |
| 300 | |a 115 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a München, Univ., Diss., 2012 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |o urn:nbn:de:bvb:19-139348 |
| 856 | 4 | |u https://nbn-resolving.org/urn:nbn:de:bvb:19-139348 |x Resolving-System | |
| 856 | 4 | 1 | |u http://edoc.ub.uni-muenchen.de/13934/ |x Verlag |z kostenfrei |3 Volltext |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024956245&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 912 | |a ebook | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-024956245 | ||
Datensatz im Suchindex
| _version_ | 1804149049412026368 |
|---|---|
| adam_text | 1
ABBILDUNGSVERZEICHNIS
......................................................................................................................................
6
2
ABKUERZUNGEN
.........................................................................................................................................................
7
3
EINLEITUNG
...............................................................................................................................................................
9
3.1
K
LASSIFIKATION
VON
H
IRNTUMOREN
............................................................................................................................
9
3.2
G
LIOBLASTOMA
MULTIFORME
.....................................................................................................................................
9
3.2.1
EPIDEMIOLOGIE
............................................................................................................................................
9
3.2.2
PATHOGENESE UND
HISTOLOGIE
...................................................................................................................
10
3.2.3
KLINIK
..........................................................................................................................................................
11
3.2.4
DIAGNOSTIK
.................................................................................................................................................
11
3.2.5
THERAPIE
....................................................................................................................................................
12
3.3
H
UMANE
MESENCHYMALE
S
TAMMZELLEN
..................................................................................................................
13
3.4
E
XPERIMENTELLE
T
HERAPIEOPTIONEN
.........................................................................................................................
14
3.5
G
RUNDLEGENDE
ZELLPHYSIOLOGISCHE
E
IGENSCHAFTEN
...............................................................................................
15
3.5.1
GAP
JUNCTIONS
............................................................................................................................................
16
3.5.2
ZELLFUSION
...................................................................................................................................................
17
4
FRAGESTELLUNG
....................................................................................................................................................18
5
MATERIAL
...............................................................................................................................................................19
5.1
Z
ELLVERSUCHE
......................................................................................................................................................
19
5.1.1
GERAETE
UND
LABORHILFEN
...........................................................................................................................
19
5.1.2
VERBRAUCHMATERIALIEN
............................................................................................................................
21
5.1.3
REAGENZIEN
...............................................................................................................................................
22
5.1.4
ZELLKULTUR
MEDIEN
UND
ZUSAETZE
..............................................................................................................
24
5.1.5
ANTIKOERPER
................................................................................................................................................
25
5.1.6
ZELLLINIEN
....................................................................................................................................................
25
5.2
T
IEREXPERIMENTE
..................................................................................................................................................27
5.2.1
GERAETE
.......................................................................................................................................................
27
5.2.2
VERBRAUCHSMATERIALIEN
...........................................................................................................................
27
5.2.3
MEDIKAMENTE
...........................................................................................................................................
28
5.2.4
VERSUCHSTIERE
...........................................................................................................................................
28
6
METHODEN
.............................................................................................................................................................29
6.1
Z
ELLBIOLOGISCHE
U
NTERSUCHUNGEN
......................................................................................................................
29
6.1.1
MEDIUMWAHL
............................................................................................................................................
29
6.1.2
KULTIVIEREN
VON
ZELLEN
.............................................................................................................................
31
6.1.3
BESTIMMUNG
DER
ZELLZAHL
........................................................................................................................
31
6.1.4
EINFRIEREN,
LAGERUNG
UND
AUFTAUEN
VON
ZELLEN
...................................................................................
32
6.1.5
GELATINEBESCHICHTUNG
VON
ZELLKULTURFLASCHEN
.....................................................................................
33
6.1.6
HERSTELLUNG
VON
STAMMZELLKONDITIONIERTEM
MEDIUM
........................................................................
33
6.1.7
ISOLATION
VON
GB-ECS,
BMMSCS
UND
GBMSCS
.......................................................................................
33
6.1.7.1
ISOLATION
VON
GB-ECS
...................................................................................................................................
33
6.1.7.2
ISOLATION
VON
HUMANEN
MESENCHYMALEN
STAMMZELLEN
..........................................................................
35
6.1.7.2.1
ISOLATION
VON
HUMANEN
MESENCHYMALEN
STAMMZELLEN
AUS
KNOCHENMARK....................................
35
6.1.7.2.2
ISOLATION
VON
HUMANEN
MESENCHYMALEN
STAMMZELLEN
AUS
TUMORRESEKTATEN
..............................
37
6.1.8
IN
VITRO
FAERBEMETHODEN
........................................................................................................................
38
6.1.8.1
CALCEIN
AM
ZYTOPIASMAFAERBUNG
..................................................................................................................
38
6.1.8.2
LIPOPHILIC
TRACER
MEMBRANFAERBUNG
.............................................................................................................
39
6.1.8.3
DEXTRAN-TETRAMETHYLRHODAMINE
ZYTOPLASMAFAERBUNG
..............................................................................
39
6.1.8.4
QUANTUM
DOTS
ZYTOPLASMAFAERBUNG
.............................................................................................................
40
6.1.8.5
ORGANELLE
LIGHTS
TRANSFIZIERUNG
...................................................................................................................
41
6.1.8.6
FIXIERUNG
UND
DAPI
KERNFAERBUNG
.................................................................................................................
42
6.1.9
MIKROSKOPIE
...........................................................................................................................................
42
6.1.9.1
DURCHLICHTMIKROSKOPIE
.................................................................................................................................
43
6.1.9.?
PHASENKONTRASTMIKROSKOPIE........................................................................................................................
43
6.1.9.3
DIC-MIKROSKOPIE
............................................................................................................................................
43
3
6.1.9.4
FLUORESZENZMIKROSKOPIE
................................................................................................................................
43
6.1.9.5
KOMBINATIONSMOEGLICHKEITEN
.........................................................................................................................
44
6.1.10
TUBE
FORMATION
ASSAY
.......................................................................................................................
44
6.1.10.1
DURCHFUEHRUNG
..................................................................................................................................................
44
6.1.10.2
QUANTIFIZIERUNG
..............................................................................................................................................
45
6.1.11
KO-KULTUR
..............................................................................................................................................
45
6.1.11.1
DURCHFUEHRUNG
MIT
VERSCHIEDENEN
FAERBETECHNIKEN
...................................................................................
45
6.1.11.2
QUANTIFIZIERUNG
..............................................................................................................................................
46
6.1.12
SPHAEROIDKULTUR
....................................................................................................................................
46
6.1.12.1
ANLEGEN
VON
SPHAEROIDEN
..............................................................................................................................47
6.1.12.1.1
SPHAEROIDE
AUF
AGAR-BESCHICHTETEN
BOEDEN
...........................................................................................
47
6.1.12.1.2
SPHAEROIDE
IM
HAENGENDEN
TROPFEN
........................................................................................................47
6.1.12.2
CALCEIN-STAMMZELLSPHAEROID
AUF
TUMORZELLRASEN
.......................................................................................
48
6.1.12.3
MIGRATIONSASSAY
MIT
SPHAEROIDEN
..................................................................................................................
48
6.1.12.3.1
DURCHFUEHRUNG
............................................................................................................................................48
6.1.12.3.2
QUANTIFIZIERUNG
........................................................................................................................................49
6.1.13
BLOCKIERUNG
DER
GAP
JUNCTION
KOMMUNIKATION
DURCH
GLYCYRRHETININSAEURE
(AGA)
....................
49
6.1.14
IMMUNZYTOCHEMIE
..............................................................................................................................
50
6.1.15
WESTERN
BLOT
........................................................................................................................................
51
6.1.15.1
VERWENDETE
LOESUNGEN
...................................................................................................................................
51
6.1.15.2
PROTEINISOLATION
..............................................................................................................................................
52
6.1.15.3
PROTEINBESTIMMUNG
NACH
BRADFORD
.............................................................................................................
52
6.1.15.4
CHEMILUMINESZENZ
.........................................................................................................................................
53
6.1.16
IMMUNZYTOCHEMIE
EINER
CO-KULTUR
..................................................................................................
54
6.2
T
IEREXPERIMENTELLE
U
NTERSUCHUNGEN
..................................................................................................................
54
6.2.1
ORTHOTOPE
TUMORIMPLANTATION
INTRAKRANIELL
.......................................................................................
56
6.2.2
MRT-KONTROLLE
..........................................................................................................................................
57
6.2.3
I.V.-GABE
VON
HMSC
.................................................................................................................................
57
6.2.4
I.P.-GABE
VON
GANCICLOVIR
.......................................................................................................................
58
6.2.5
PRAEPARATION
DER
HIRNRESEKTATE
..............................................................................................................
58
6.2.6
AUFARBEITUNG
DER
HIRNRESEKTATE
............................................................................................................
58
6.2.7
HE-FAERBUNG
DER
HIRNPRAEPARATE
.............................................................................................................
59
6.2.8
IMMUNHISTOCHEMIE
DER
HIRNPRAEPARATE
................................................................................................
59
7
ERGEBNISSE
............................................................................................................................................................
61
7.1
M
ODIFIZIERTE
,
NEUARTIGE
M
ETHODEN
.....................................................................................................................61
7.1.1
TUBE
FORMATION
ASSAY
MIT
ENDOTHELZELLEN
UND
HMSCS
......................................................................
61
7.1.2
SPHAEROIDE
AUS
TUMORZELLEN
UND
HMSCS
...............................................................................................
65
7.2
I
NTERAKTION
VON
G
LIOMZELLEN
UND
H
MSC
S
ALS
FUNKTIONELLES
S
YNZYTIUM
.............................................................
68
7.2.1
GAP
JUNCTION
VERMITTELTER
AUSTAUSCH
VON
CALCEIN
IN
CO-KULTUREN
VON
GLIOM
UND
STAMMZELLEN
68
7.2.2
GAP
JUNCTION
INHIBITION
MITTELS
GLYCYRRHETININSAEURE
(AGA)
...............................................................
71
7.2.3
CONNEXIN-NACHWEIS
MITTELS
IMMUNZYTOCHEMIE
...................................................................................
73
7.2.4
CONNEXIN-NACHWEIS
MITTELS
WESTERN
BLOT
............................................................................................
74
7.3
I
NTERAKTION
VON
G
LIOMZELLEN
UND
H
MSC
S
ALS
STRUKTURELLES
S
YNZYTIUM
.............................................................
76
7.3.1
FUSIONS-NACHWEIS
MITTELS
LIPOPHILIC
TRACERS........................................................................................
76
7.3.2
FUSIONS-NACHWEIS
MITTELS
DEXTRAN-RHODAMINE
..................................................................................
79
7.3.3
FUSIONS-NACHWEIS
MITTELS
QUANTUM
DOTS
............................................................................................
80
7.3.4
FUSIONS-NACHWEIS
MITTELS
ORGANELLE
LIGHTS
.........................................................................................
82
7.3.5
FUSIONS-NACHWEIS
MITTELS
IMMUNZYTOCHEMIE
5V40
............................................................................
82
7.4
T
IEREXPERIMENTELLE
U
NTERSUCHUNGEN
...................................................................................................................
84
7.4.1
VERLAUF
.......................................................................................................................................................
84
7.4.2
VERLAUFSKONTROLLE
MITTELS
MRT-SCAN
.....................................................................................................
84
7.4.3
IMMUNHISTOCHEMIE
DER
HIRNPRAEPARATE
................................................................................................
86
8
DISKUSSION
............................................................................................................................................................
87
8.1
H
MSC
S
TRAGEN
ZUR
N
EOANGIOGENESE
EINES
G
LIOMS
BEI
..........................................................................................
87
8.2
F
UNKTIONELLES
S
YNZYTIUM
AUS
G
LIOM
-
UND
S
TAMMZELLEN
......................................................................................88
8.3
S
TRUKTURELLES
S
YNZYTIUM
AUS
G
LIOM
-
UND
S
TAMMZELLEN
......................................................................................91
8.4
HMSCS
TRAGEN
ZUR
TUMORIGENITAET
BEI
.................................................................................................................
92
4
8.5
E
INSATZ
VON
GENETISCH
MODIFIZIERTEN
H
MSC
S
SINNVOLL
........................................................................................
94
8.6
D
ER
S
CHRITT
VON
IN
VITRO
ZU
IN
VIVO
.....................................................................................................................95
9
ZUSAMMENFASSUNG
............................................................................................................................................
96
10
LITERATURVERZEICHNIS
........................................................................................................................................
97
11
DANKSAGUNG
......................................................................................................................................................
114
5
|
| any_adam_object | 1 |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV040099668 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T00:16:51Z |
| institution | BVB |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-024956245 |
| oclc_num | 794529101 |
| open_access_boolean | 1 |
| owner | DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 |
| owner_facet | DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 |
| physical | 115 S. Ill., graph. Darst. |
| psigel | ebook |
| publishDate | 2012 |
| publishDateSearch | 2012 |
| publishDateSort | 2012 |
| record_format | marc |
| spelling | Korte, Benjamin Verfasser (DE-588)1020531258 aut Interaktionsmuster von Gliomzellen, Gliomendothelzellen und humanen mesenchymalen Stammzellen von Benjamin Korte 2012 115 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Univ., Diss., 2012 (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:19-139348 https://nbn-resolving.org/urn:nbn:de:bvb:19-139348 Resolving-System http://edoc.ub.uni-muenchen.de/13934/ Verlag kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024956245&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Korte, Benjamin Interaktionsmuster von Gliomzellen, Gliomendothelzellen und humanen mesenchymalen Stammzellen |
| subject_GND | (DE-588)4113937-9 |
| title | Interaktionsmuster von Gliomzellen, Gliomendothelzellen und humanen mesenchymalen Stammzellen |
| title_auth | Interaktionsmuster von Gliomzellen, Gliomendothelzellen und humanen mesenchymalen Stammzellen |
| title_exact_search | Interaktionsmuster von Gliomzellen, Gliomendothelzellen und humanen mesenchymalen Stammzellen |
| title_full | Interaktionsmuster von Gliomzellen, Gliomendothelzellen und humanen mesenchymalen Stammzellen von Benjamin Korte |
| title_fullStr | Interaktionsmuster von Gliomzellen, Gliomendothelzellen und humanen mesenchymalen Stammzellen von Benjamin Korte |
| title_full_unstemmed | Interaktionsmuster von Gliomzellen, Gliomendothelzellen und humanen mesenchymalen Stammzellen von Benjamin Korte |
| title_short | Interaktionsmuster von Gliomzellen, Gliomendothelzellen und humanen mesenchymalen Stammzellen |
| title_sort | interaktionsmuster von gliomzellen gliomendothelzellen und humanen mesenchymalen stammzellen |
| topic_facet | Hochschulschrift |
| url | https://nbn-resolving.org/urn:nbn:de:bvb:19-139348 http://edoc.ub.uni-muenchen.de/13934/ http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024956245&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT kortebenjamin interaktionsmustervongliomzellengliomendothelzellenundhumanenmesenchymalenstammzellen |